检索结果-南通市图书馆

Bacteria colonization and gene expression related to immune function in colon mucosa is associated with growth in neonatal calves regardless of live yeast supplementation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Animal Science and Biotechnology 2024年第5期15卷 1980-1995页

作者： Koki Nishihara Clothilde Villot Lautaro Cangiano Le Luo Guan Michael Steele department of Animal biosciences Animal Science and NutritionUniversity of GuelphGuelphON N1G 1Y2Canada department of Agricultural Food and Nutritional ScienceUniversity of AlbertaEdmonton T6G 2P5Canada Lallemand SAS Blagnac F‑31702France department of Animal and Dairy Sciences University of Wisconsin-MadisonMadisonWIUSA Faculty of Land and Food Systems The University of British ColumbiaVancouverBC V6T 1Z4Canada Present Address:department of Animal biosciences University of GuelphGuelphON N1G 2W1Canada

Background As Holstein calves are susceptible to gastrointestinal disorders during the first week of life,understanding how intestinal immune function develops in neonatal calves is important to promote better intestinal *** probiotics in early life may contribute to host intestinal health by facilitating beneficial bacteria colonization and developing intestinal immune *** objective of this study was to characterize the impact of early life yeast supplementation and growth on colon mucosa-attached bacteria and host immune *** Twenty Holstein bull calves received no supplementation(CON)or Saccharomyces cerevisiae boulardii(SCB)from birth to 5 d of *** tissue biopsies were taken within 2 h of life(D0)before the first colostrum feeding and 3 h after the morning feeding at d 5 of age(D5)to analyze mucosa-attached bacteria and colon *** sequencing showed that there was no difference inαandβdiversity of mucosa-attached bacteria between day and treatment,but bacteria related to diarrhea were more abundant in the colon mucosa on D0 compared to *** addition,q PCR indicated that the absolute abundance of Escherichia coli(***)decreased in the colon mucosa on D5 compared to D0;however,that of Bifidobacterium,Lactobacillus,and Faecalibacterium prausnitzii,which could competitively exclude ***,increased in the colon mucosa on D5 compared to ***-sequencing showed that there were no differentially expressed genes between CON and SCB,but suggested that pathways related to viral infection such as“Interferon Signaling”were activated in the colon mucosa of D5 compared to *** Growth affected mucosa-attached bacteria and host immune function in the colon mucosa during the first 5 d of life in dairy calves independently of SCB *** early life,opportunistic pathogens may decrease due to intestinal environmental changes by beneficial bacteria and/or host immune *** activation

关键词： Colon biopsy Dairy calves RNA-sequencing Saccharomyces cerevisiae boulardii 16S rRNA amplicon-sequencing

维普期刊数据库

Telomeric DNA breaks in human induced pluripotent stem cells trigger ATR-mediated arrest and telomerase-independent telomere damage repair

在线全文

学校读者我要写书评

暂无评论

Journal of Molecular Cell Biology 2024年第3期16卷 46-60页

作者： Katrina N.Estep John W.Tobias Rafael J.Fernandez Brinley M.Beveridge F.Brad Johnson department of Pathology and Laboratory Medicine University of PennsylvaniaPhiladelphiaPA 19104USA Quantiative biosciences Merck&Co.Inc.West PointPA 19486USA Penn Genomic Analysis Core University of PennsylvaniaPhiladelphiaPA 19104USA

Although mechanisms of telomere protection are well-defined in differentiated cells,how stem cells sense and respond to telomere dysfunction,in particular telomeric double-strand breaks(DSBs),is poorly ***,we report the DNA damage signaling,cell cycle,and transcriptome changes in human induced pluripotent stem cells(iPSCs)in response to telomere-internal *** engineer human iPSCs with an inducible TRF1-FokI fusion protein to acutely induce DSBs at *** this model,we demonstrate that TRF1-FokI DSBs activate an ATR-dependent DNA damage response,which leads to p53-independent cell cycle arrest in *** CRISPR–Cas9 to cripple the catalytic domain of telomerase reverse transcriptase,we show that telomerase is largely dispensable for survival and lengthening of TRF1-FokI-cleaved telomeres,which instead are effectively repaired by robust homologous recombination(HR).In contrast to HR-based telomere maintenance in mouse embryonic stem cells,where HR causes ZSCAN4-dependent extension of telomeres beyond their initial lengths,HR-based repair of telomeric breaks is sufficient to maintain iPSC telomeres at a normal length,which is compatible with sustained survival of the cells over several days of TRF1-FokI *** findings suggest a previously unappreciated role for HR in telomere maintenance in telomerase-positive iPSCs and reveal distinct iPSC-specific responses to targeted telomeric DNA damage.

关键词： telomeres telomerase alternative lengthening of telomeres pluripotent stem cells DNA damage double-strand breaks

Lighting up aggregate emission of perylene diimide by leveraging polymerization-mediated through-space charge transfer and π-π stacking

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China Chemistry 2024年

作者： Suiying Ye Désirée Füglistaller Tian Tian Anjay Manian Sudhir Kumar Celine Nardo Andrew J.Christofferson Salvy P.Russo Chih-Jen Shih Jean-Christophe Leroux Yinyin Bao Institute of Pharmaceutical Sciences Department of Chemistry and Applied Biosciences ETH Zurich Vladimir-Prelog-Weg 3 Institute for Chemical and Bioengineering Department of Chemistry and Applied Biosciences ETH Zurich Vladimir-Prelog-Weg 1-5 ARC Centre of Excellence in Exciton Science School of Science RMIT University School of Science STEM College RMIT University

The molecular engineering of fluorescent organic/polymeric materials, specifically those emitting in the deep red to near-infrared spectrum, is vital for advancements in optoelectronics and biomedicine. Perylene diimide(PDI), a well-known fluorescent scaffold, offers high thermal and photophysical stability but suffers from fluorescence quenching in solid or aggregate states due to intense π-π interactions. To mitigate this, simple and versatile methods for strong PDI aggregate emission without extensive synthetic demands are highly desirable but still lacking. Here, we report a straightforward strategy to enhance the solid-state emission of PDI by introducing certain degree of through-space charge transfer(TSCT) via controlled radical polymerization,which can efficiently distort the typical face-to-face PDI stacking, enabling greatly enhanced deep red emission. This is achieved by growing electron-donating star-shape styrenic(co)polymers from a multidirectional electron-accepting PDI initiator. The incorporation of polycyclic aromatic monomers further shifted the emission into the near-infrared region, albeit with a reduced intensity. Overall, the emission of the PDI-based TSCT polymers can be systematically manipulated by leveraging the balance between PDI stacking and the TSCT degree, as confirmed by both experimental study and theoretical calculations. Our approach circumvents complex synthetic procedures, offering highly emissive materials with large Stokes shifts and showing broad potential for optoelectronic technology.

关键词：

Innovative COVID-19 Screening: RT-LAMP Assay for Spike and NSP1 Proteins

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Journal of Clinical and Nursing Research 2024年第6期8卷 256-265页

作者： Muhammad Yousaf Amber Khan department of biosciences COMSATS University IslamabadPark RdIslamabad Capital Territory 45550Pakistan department of biosciences Shaheed Zulfikar Ali Bhutto Institute of Science and Technology(SZABIST)KarachiPakistan

Coronavirus disease(COVID-19)is a serious respiratory disease that spreads through the coronavirus *** soon became a pandemic after its appearance in 2019 and demanded new techniques for its identification and *** to this situation,RT-LAMP appears to be a novel method for the identification of COVID-19 because of its vast applications,including cost-effectiveness and *** research highlights the use of RT-LAMP,a more sensitive test than RT-PCR,for the assessment of SARS-CoV-2,the severe acute respiratory *** identify the spike(S)and NSP1 protein using RT-LAMP,170 total samples of coronavirus-suspected patients were served in this *** certifications and bioethical considerations were taken into *** the sample was extracted from the patient's swabs,RNA was isolated,extracted,and *** response was then run on the RT-LAMP at the ideal temperature,and the outcomes could be observed with the unaided eye as they changed from pink to *** is a simple method of determining if the test is positive or *** this purpose,both RT-LAMP and RT-PCR tests are used during these *** linked with COVID-19 testing including S,nspl,and ORF are suited to coronavirus testing;they have 100%specificity and low sensitivity,but S has more specificity and sensitivity than nspl and ORF,*** of the 95 positive samples,89(93.68%)samples yielded favorable outcomes utilizing RT-LAMP,while 55 negative samples yielded 100%positive *** present research demonstrates that RT-LAMP is less sensitive yet more selective for coronavirus detection.

关键词： COVID-19 NSP1 Spike RT-LAMP

Roles of Mutant TP53 Gene in Cancer Development and Progression

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Proceedings of Anticancer Research 2024年第5期8卷 165-181页

作者： Muhammad Abubakar Baqaur Rehman department of biosciences COMSATS University IslamabadIslamabad Capital Territory 45550Pakistan

TP53 is a tumor suppressor gene that is mutated in most cancer types and has been extensively studied in cancer research.p53 plays a critical role in regulating the expression of target genes and is involved in key processes such as apoptosis,cell cycle regulation,and genomic stability,earning it the title“guardian of the genome.”Numerous studies have demonstrated p53’s influence on and regulation of autophagy,ferroptosis,the tumor microenvironment,and cell metabolism,all of which contribute to tumor *** in p53,specifically mutant p53(mutp53),not only impair its tumor-suppressing functions but also enhance oncogenic *** data indicate that mutp53 is strongly associated with poor prognosis and advanced cancers,making it an ideal target for the development of novel cancer *** review summarizes the post-translational modifications of p53,the mechanisms of mutp53 accumulation,and its gain-of-function,based on previous ***,this review discusses its impact on metabolic homeostasis,ferroptosis,genomic instability,the tumor microenvironment,and cancer stem cells,and highlights recent advancements in mutp53 research.

关键词： p53 Cancer Mutant p53(mutp53) Progression Treatment

Analysis of causes for revision in unicompartmental knee arthroplasty

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Clinical Cases 2024年第25期12卷 5642-5645页

作者： Sujit Kumar Tripathy Siddharth Satyakam Pradhan Shahnawaz Khan Hursch Patel department of Orthopaedics All India Institute of Medical SciencesBhubaneswar 751019OdishaIndia department of Orthopedics All India Institute of Medical SciencesBhubaneswar 751019India department of Medicine Kansas City University of Medicine and BiosciencesKansas CityMO 64106United States

The study by Zhao et al identifies the factors leading to the failure of unicompartmental knee arthroplasty(UKA)in their *** factors include substandard suturing of the wound,the presence of osteophytes and intra-articular loose bodies causing impingement,premature loosening of the tibial component,choosing unsuitable patients for the procedure,dislocation of the movable insert,and damage to the anterior cruciate ligament and medial collateral *** findings suggest that employing the correct surgical techniques and indications is essential for successful outcomes in the UKA.

关键词： Unicompartmental knee arthroplasty Arthroplasty Knee Failure Conversion TKA

Polyethylene glycol has immunoprotective effects on sciatic allografts, but behavioral recovery and graft tolerance require neurorrhaphy and axonal fusion

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第4期20卷 1192-1206页

作者： Tyler A.Smith Liwen Zhou Cameron L.Ghergherehchi Michelle Mikesh Cathy Z.Yang Haley O.Tucker JuliAnne Allgood Jared S.Bushman George D.Bittner department of Molecular biosciences University of Texas at AustinAustinTXUSA department of Neuroscience University of Texas at AustinAustinTXUSA Division of Pharmaceutical Sciences University of WyomingLaramieWYUSA

Behavioral recovery using(viable)peripheral nerve allografts to repair ablation-type(segmental-loss)peripheral nerve injuries is delayed or poor due to slow and inaccurate axonal ***,such peripheral nerve allografts undergo immunological rejection by the host immune *** contrast,peripheral nerve injuries repaired by polyethylene glycol fusion of peripheral nerve allografts exhibit excellent behavioral recovery within weeks,reduced immune responses,and many axons do not undergo Wallerian *** relative contribution of neurorrhaphy and polyethylene glycol-fusion of axons versus the effects of polyethylene glycol per se was unknown prior to this *** hypothesized that polyethylene glycol might have some immune-protective effects,but polyethylene glycol-fusion was necessary to prevent Wallerian degeneration and functional/behavioral *** examined how polyethylene glycol solutions per se affect functional and behavioral recovery and peripheral nerve allograft morphological and immunological responses in the absence of polyethylene glycol-induced axonal ***-type sciatic nerve injuries in outbred Sprague–Dawley rats were repaired according to a modified protocol using the same solutions as polyethylene glycol-fused peripheral nerve allografts,but peripheral nerve allografts were loose-sutured(loose-sutured polyethylene glycol)with an intentional gap of 1–2 mm to prevent fusion by polyethylene glycol of peripheral nerve allograft axons with host *** to negative control peripheral nerve allografts not treated by polyethylene glycol and in contrast to polyethylene glycol-fused peripheral nerve allografts,animals with loose-sutured polyethylene glycol peripheral nerve allografts exhibited Wallerian degeneration for all axons and myelin degeneration by 7 days postoperatively and did not recover sciatic-mediated behavioral functions by 42 days *** morphological signs of rejection,such as col

关键词： allograft rejection axotomy macrophage myelin nerve repair polyethylene glycol(PEG) sciatic nerve T cell transplantation Wallerian degeneration

维普期刊数据库

Exploring the Pivotal Association of AI in Cancer Stem Cells Detection and Treatment

在线全文

学校读者我要写书评

暂无评论

Proceedings of Anticancer Research 2024年第5期8卷 52-63页

作者： Muhammad Abubakar department of biosciences COMSATS University IslamabadPark RdIslamabad Capital Territory 45550Pakistan

Cancer stem cells(CSCs),or tumor-initiating cells(TICs),are cancerous cell subpopulations that remain while tumor cells propagate as a unique subset and exhibit multiple applications in several *** are responsible for cancer cell initiation,development,metastasis,proliferation,and recurrence due to their self-renewal and differentiation abilities in many kinds of *** intelligence(AI)has gained significant attention because of its vast applications in various fields including agriculture,healthcare,transportation,and robotics,particularly in detecting human diseases such as *** division and metastasis of cancerous cells are not easy to identify at early stages due to their uncontrolled *** has provided some real-time pictures of cancer progression and *** purpose of this review paper is to explore new investigations into the role of AI in cancer stem cell progression and metastasis and in regenerative *** describes the association of machine learning and AI with CSCs along with its numerous applications from cancer diagnosis to *** review has also provided key challenges and future directions of AI in cancer stem cell research diagnosis and therapeutic approach.

关键词： Artificial intelligence Cancer Cancer stem cells Health

New indices to balanceα-diversity against tree size inequality

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Forestry Research 2024年第1期35卷 229-237页

作者： Li Zhang Brady K.Quinn Cang Hui Meng Lian Johan Gielis Jie Gao Peijian Shi College of Ecology and Environment Bamboo Research InstituteNanjing Forestry University159 Longpan RoadNanjing 210037People’s Republic of China Fisheries and Oceans Canada Andrews Biological Station125 Marine Science DriveSt.AndrewsNB E5B 0E4Canada department of Mathematical Sciences Centre for Invasion BiologyStellenbosch UniversityMatieland 7602South Africa Mathematical and Physical biosciences African Institute for Mathematical SciencesCape Town 7945South Africa department of biosciences Engineering University of AntwerpB-2020 AntwerpBelgium College of Life Science Xinjiang Normal University102 Xinyi RoadUrumqi 830054People’s Republic of China

The number and composition of species in a community can be quantified withα-diversity indices,including species richness(R),Simpson’s index(D),and the Shannon-Wiener index(H΄).In forest communities,there are large variations in tree size among species and individu-als of the same species,which result in differences in eco-logical processes and ecosystem ***,tree size inequality(TSI)has been largely neglected in studies using the available diversity *** TSI in the diameter at breast height(DBH)data for each of 99920 m×20 m forest census quadrats was quantified using the Gini index(GI),a measure of the inequality of size *** generalized performance equation was used to describe the rotated and right-shifted Lorenz curve of the cumulative proportion of DBH and the cumulative proportion of number of trees per *** also examined the relationships ofα-diversity indices with the GI using correlation *** generalized performance equation effectively described the rotated and right-shifted Lorenz curve of DBH distributions,with most root-mean-square errors(990 out of 999 quadrats)being<*** were significant positive correlations between each of threeα-diversity indices(i.e.,R,D,and H’)and the ***,the total abundance of trees in each quadrat did not significantly influence the *** means that the TSI increased with increasing spe-cies ***,two new indices are proposed that can balanceα-diversity against the extent of TSI in the com-munity:(1−GI)×D,and(1−GI)×H’.These new indices were significantly correlated with the original D and H΄,and did not increase the extent of variation within each group of *** study presents a useful tool for quantifying both species diversity and the variation in tree sizes in forest communities,especially in the face of cumulative species loss under global climate change.

关键词： Diameter at breast height(DBH) Gini index Shannon-Wiener index Simpson’s index Quadrat Tree size

维普期刊数据库