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A brief review of novel nucleic acid test biosensors and their application prospects for salmonids viral diseases detection

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Journal of Semiconductors 2023年第2期44卷 49-56页

作者： Xiaofei Liu Songyin Qiu Haiping Fang Lin Mei Hongli Jing Chunyan Feng Shaoqiang Wu Xiangmei Lin Institute of Animal inspection and quarantine Chinese Academy of Inspection and QuarantineBeijing 100176China Research Center of GACC for international inspection and quarantine Standards and Technical Regulations Beijing 100013China

Viral diseases represent one of the major threats for salmonids *** detection and identification of viral pathogens is the main prerequisite prior to undertaking effective prevention and control ***,sensitive,efficient and portable detection method is highly essential for fish viral diseases *** strategies are highly prevalent and fulfill the expanding demands of on-site detection with fast response,cost-effectiveness,high sensitivity,and *** the development of material science,the nucleic acid biosensors fabricated by semiconductor have shown great potential in rapid and early detection or screening for diseases at salmonids *** paper reviews the current detection development of salmonids viral *** present limitations and challenges of salmonids virus diseases surveillance and early detection are *** nucleic acid semiconductor biosensors are briefly *** perspective and potential application of biosensors in the on-site detection of salmonids diseases are discussed.

关键词： salmonids virus detection nucleic acid test biosensors semiconductor

Synthesis, Characterization and Crystal Structure of (+)-N-[(3S)-3-(4- fluorophenyl)heptanoyl]bornane-10,2-sultam

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Chinese Journal of Structural Chemistry 2012年第9期31卷 1247-1250页

作者：韩勰汪淼李菲徐媛原曹秀芳 College of Science Huazhong Agricultural University inspection and quarantine Technology Center Hainan Entry-exit Inspection and Quarantine Bureau

The chiral molecule （＋）-N-[（3S）-3-（4-fluorophenyl）heptanoyl]bornane-10,2-sultam （C23H32FNO3S, Mr = 421.56）, a fluorine-containing derivative of camphorsultam, was conveniently obtained and crystallized in the orthorhombic space group P212121 with a = 7.9044（11）, b = 11.6680（16）, c = 24.899（3） , V = 2296.4（5） 3, Z = 4, Dc = 1.219 Mg/m3, λ = 0.71073 , μ（MoKα） = 0.172 mm-1 and F（000） = 904. X-ray diffraction analysis reveals that the six-membered ring of sultam shows a boat conformation （Fig. 1）. The planes constructed by （（C6）, （C7）, （C8）, （C9）） and （（C4）, （C5）, （C6）, （C9）） form a dihedral angle of 70.3（3）°. The plane of （C1）-（C2）-（C3） forms dihedral angles to the aforementioned planes of 85.9（4） and 89.5（4）°, respectively. The molecules are linked via C-H···O/F interactions.

关键词： (+)-N- [(3S)-3-(4-fluorophenyl)heptanoyl] bornane-10,2-sultam,synthesis crystal structure

Maternal and fetal death associated with acute pancreatitis during pregnancy:A case report

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World Journal of Clinical Cases 2025年第10期13卷 53-58页

作者： Jin Li Qing-Yan Zhang Mei-Hong Zhang Shan-Yun Jiang department of Anesthesiology Kaihua County People’s HospitalQuzhou 324300Zhejiang ProvinceChina Center for Clinical inspection and quarantine Kaihua County Center for Clinical Inspection and QuarantineQuzhou 324300Zhejiang ProvinceChina

BACKGROUND Acute pancreatitis in pregnancy is a rare but serious condition that can lead to high maternal mortality and fetal *** of pregnancy complicated by severe acute pancreatitis,particularly with subsequent respiratory and cardiac arrest,are rarely *** SUMMARY We present the case of a 35-year-old woman,at 36+5 weeks of gestation,who presented with paroxysmal epigastric pain accompanied by low back pain,nausea,and *** to the clinical symptoms,B-ultrasound imaging and biochemical indicators,the patient was diagnosed with acute pancreatitis and initially managed ***,3 hours after admission,the patient experienced respiratory and cardiac arrest,and the fetus *** this case,the adverse outcomes occurred due to the lack of aggressive fluid resuscitation and an active surgical *** Implementing aggressive fluid resuscitation to sustain tissue perfusion,alongside the proactive evaluation of pharmacological agents that suppress gastric acid secretion and inhibit pancreatic enzyme activity,may be beneficial in mitigating the risk of a severely adverse *** management of acute pancreatitis during pregnancy requires careful timing of surgical intervention,a thorough evaluation of the risks and benefits regarding the continuation or termination of pregnancy,and a focus on safeguarding both maternal and fetal health.

关键词： Severe acute pancreatitis Pregnancy Cardiac arrest Fetal death Case report

Advanced biosensing technologies for monitoring of agriculture pests and diseases:A review

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Journal of Semiconductors 2023年第2期44卷 57-65页

作者： Jiayao He Ke Chen Xubin Pan Junfeng Zhai Xiangmei Lin Chinese Academy of inspection and quarantine Beijing 100176China

The threat posed to crop production by pests and diseases is one of the key factors that could reduce global food *** detection is of critical importance to make accurate predictions,optimize control strategies and prevent crop *** technological advancements highlight the opportunity to revolutionize monitoring of pests and *** methodologies offer potential solutions for real-time and automated monitoring,which allow advancements in early and accurate detection and thus support sustainable crop ***,advanced biosensing technologies for pests and diseases monitoring,including image-based technologies,electronic noses,and wearable sensing methods are ***,challenges and future perspectives for widespread adoption of these technologies are ***,we believe it is necessary to integrate technologies through interdisciplinary cooperation for further exploration,which may provide unlimited possibilities for innovations and applications of agriculture monitoring.

关键词： precision agriculture biosensors crops disease and pest management

Antimicrobial Susceptibility and Characterization of Outer Membrane Proteins of Aeromonas hydrophila Isolated in China

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Journal of Integrative Agriculture 2014年第4期13卷 911-917页

作者： GUO Peng WANG Na LIU Yong-jie LU Cheng-ping College of Veterinary Medicine Nanjing Agricultural University Institute of Animal quarantine Chinese Academy of Inspection and Quarantine

Aeromonas hydrophila isolates from clinical cases （n=43） were tested against 8 antimicrobial agents and typed by outer membrane protein （OMP） pattern by using sodium dodecyl sulfate gel electrophoresis. All isolates were resistant to ampicillin （MICs, ≥16 μg mL-1） and sulfamonomethoxine （MICs≥64 μg mLl）, but susceptible to norfloxacin （MICs,≤0.5 μg mL-1）. There was a high incidence of resistance to erythromycin （90.70%） and tylosin （93.02%）, while a low incidences of resistance to ciprofloxacin （2.33%）, enrofloxacin （2.33%） and florfenicol （4.65%）. Six different outer membrane protein patterns were found among 34 isolates by analyzing proteins in the range of 22 to 50 kDa, other than 9 isolates with their respective profiles. The strains with the similar OMP profiles had similar resistances. Compared with the other strains from the same OMP patterns, NB-1, *** and MR-1 had lacked the proteins in the range of 30 to 45 kDa and their resistance to florfenicol substantially increased. It is speculated that the outer membrane protein changes might correlate with decreased susceptibility to florfenicol in the three strains. Some strains which showed completely identical OMP types had a little difference in their resistance to fluoroquinolones, indicating that there might be other factors that were involved in the antimicrobial resistance of A. hydrophila.

关键词： Aeromonas hydrophila antimicrobial resistance outer membrane proteins

Qualitative tool for designing natural protected areas and ecological restoration projects

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Ecosystem health and Sustainability 2019年第1期5卷 181-184,I0018页

作者： Xubin Pan Institute of Plant quarantine Chinese Academy of Inspection and QuarantineBeijingChina

The rotational symmetry connection between the Species-Area Relationship(SAR)and Endemics-Area Relationship(EAR)was discovered in the frame of Species-Area *** this paper,this paired connection was applied into the impact of variation of number of species and area and dynamic change of species-abundance distribution on the curve shape of SAR and EAR,which can be integrated into the assessment of Natural Protected Areas and Ecological Restoration *** results indicate that the underestimate or overestimate of total number of species can lead to the underestimate or overestimate of extinction rate,while the reduction or expansion of area can lead to the increase or decrease in extinction *** addition,the species-abundance distribution change of community causes the shape tuning of SAR and EAR,which leads to inconsistent change of extinction ***,this SAT frame can be used as a qualitative tool in conservation and restoration management.

关键词： Rotational symmetry extinction rate speciesabundance distribution Pan effect disturbance

Determination of Rotenone Residues in Foodstuffs by Solid-Phase Extraction (SPE) and Liquid Chromatography/Tandem Mass Spectrometry (LC-MS/MS)

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Agricultural Sciences in China 2010年第9期9卷 1299-1308页

作者： XU Dun-ming ZHOU Yu LIN Li-yi ZHANG Zhi-gang ZHANG Jin LU Sheng-yu YANG Fang HUANG Peng-ying Xiamen Entry-Exit inspection and quarantine Bureau Xiamen 361026 P.R. China Fujian Entry-Exit inspection and quarantine Bureau Fuzhou 350001 P.R.China

We developed a novel approach to determine rotenone residues in foodstuffs, by integrating solid-phase extraction （SPE） and liquid chromatography/tandem mass spectrometry （LC-MS/MS） technologies, to achieve high sensitivity and selectivity. In our method, the solvent extraction with n-hexane-dichloromethane （50：50, v/v） and cleanup with florisil SPE cartridges using ethyl acetate-ethyl ether （25：75, v/v） as eluents provided adequate recovery of rotenone. The detection of rotenone was then carried out by LC-MS/MS using acetonitrile-water with the 0.1% formic acid （w/v） as the mobile phase. The multiple reaction monitoring （MRM） scheme employed in the approach involved the transitions of the precursor ion to three selected product ions, in which one pair for quantification was m/z 395.3 〉 213.2 and the other two pairs for identification were m/z 395.3 〉 192.2 and 395.3 〉 367.0. The limits of quantification （LOQs） of the method ranged from 0.001 to 0.005 mg kg-1 depending on the matrix. Intra- and inter-day precisions （relative standard deviations, RSDs） for rotenone were less than 7.1 and 14.8%, respectively. Results from repetitive analysis suggested good reproducibility of the method for rotenone residue detection. The recoveries at three concentrations （LOQ, 10LOQ and 100LOQ） ranged from 79.3-118.3% in cabbage, potato, onion, carrot, apple, orange, banana, lichee, tea, and Shiitake mushroom. The proposed procedure was then applied to the analysis of 129 real samples collected from Xiamen, Fujian Province, China. The existence of rotenone was found in two tea products with concentrations of 0.012 and 0.016 mg kg-1, respectively. The method has great potential for routine analysis of monitoring rotenone residue in foodstuffs.

关键词： rotenone LC-MS/MS foodstuff residue SPE

The Prokaryotic Expression and Bioactivity of the Recombinant Red Fire Ant Venom Allergen Sol i 4

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Agricultural Sciences in China 2009年第2期8卷 182-187页

作者： HAN Xue-qing LIN Xiang-mei CHEN Hong-jun ZHANG Yong-guo YE Gui-sheng WU Shao-qiang LI Jian CHEN Nai-zhong CHEN Yan ZHU Shui-fang Chinese Academy of inspection and quarantine Beijing 100029 P.R.China

The sting of red imported fire ant （RIFA） could cause serious allergic response in fraction of people. These allergic reactions are mainly caused by its venom, especially venom allergen Sol i 1-4. To produce large amount of RIFA venom allergen Sol i 4 for diagnosis of RIFA allergy and allergen-specific immunotherapy, the gene encoding this protein was amplified and cloned into the prokaryotic expression vector pET43, la. The recombinant plasmid was used to transform competent cells and the recombinant proteins were expressed in E. coll. SDS-PAGE and Western blotting analysis indicated that high-level expression of Sol i 4 protein was successfully achieved. Allergenic activity analysis of the recombinant allergen Sol i 4 was then performed on rabbit. The result showed that the recombinant protein obtained had significant allergenic activity. It indicated that the recombinant allergen Sol i 4 of RIFA venom was successfully expressed in E. coli, which provided foundation for further developing therapeutic and diagnosis reagents of RIFA allergy.

关键词： red imported fire ant recombinant allergen Sol i 4 expression activity

On-chip solid phase extraction coupled with electrophoresis using modified magnetic microspheres as stationary phase

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Science China Chemistry 2009年第12期52卷 2287-2294页

作者： LI Hong LI HaiFang CHEN ZhiFeng LIN JinMing department of Chemistry Tsinghua UniversityBeijing 100084China Chinese Academy of inspection and quarantine Beijing 100123China

A poly(dimethylsiloxane)(PDMS)/glass hybrid microchip for on-line solid phase extraction (SPE) and electrophoresis separation has been developed and evaluated. The SPE microchannel was crossed to the electrophoresis microchannel. All the microfluidic channels were etched on the glass substrate. The magnetic microspheres were coated with hydroxyl-terminated poly-dimethylsiloxane (PDMS-OH) serving as extraction phase,which could be conveniently immobilized into the sample pretreatment channel by magnetic field. The PDMS-OH microspheres were mobilized into and out of the pretreatment channel by injection flow. The 0.1 μmol/L solution of fluorescence isothiocyanate (FITC)-labeled phenylalanine (Phe) was electrically injected into the SPE channel and extracted onto the PDMS-OH microspheres bed. The enriched FITC-labeled Phe was electrically eluted by 9 mmol/L sodium acetate containing 10% acetonitrile and electrically driven into the electrophoresis channel and then separated. The preconcentration factor could reach 87.5 after sufficient extraction. A linear preconcentration curve was obtained with the initial FITC-labeled Phe concentration ranging from 6 nmol/L to 300 nmol/L (R2=0.9922) with 200 s loading time. The detection limit (S/N=3) for the FITC-labeled Phe was 3 nmol/L.

关键词： microfluidic device solid phase extraction magnetic microspheres hydroxyl-terminated poly-dimethylsiloxane