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A nanobody-based blocking enzyme-linked immunosorbent assay for detecting antibodies against pseudorabies virus glycoprotein E

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Journal of Integrative Agriculture 2024年第4期23卷 1354-1368页

作者： Huanhuan Lü Pinpin Ji Siyu Liu Ziwei Zhang Lei Wang Yani Sun Baoyuan Liu Lizhen Wang Qin Zhao department of Preventive Veterinary Medicine College of Veterinary MedicineNorthwest A&F University/Shaanxi Scientific Observing and Experimental Station of Veterinary Pharmacology and Diagnostic TechnologyMinistry of Agriculture and Rural AffairsYangling 712100China

Pseudorabies(PR)is an acute infectious disease of pigs caused by the PR virus(PRV)and results in great economic losses to the pig industry *** glycoprotein E(gE)-based enzyme-linked immunosorbent assay(ELISA)has been used to distinguish gE-deleted vaccine-immunized pigs from wild-type virus-infected pigs to eradicate PR in some *** has the advantages of small size and easy genetic engineering and has been a promising diagnostic ***,there were few reports about developing nanobody-based ELISA for detecting anti-PRV-gE *** the present study,the recombinant PRV-gE was expressed with a bacterial system and used to immunize the Bactrian ***,two nanobodies against PRV-gE were screened from the immunized camel by phage display ***,two nanobody-HRP fusion proteins were expressed with HEK293T *** PRV-gE-Nb36-HRP fusion protein was selected as the probe for developing the blocking ELISA(bELISA)to detect anti-PRV-gE *** optimizing the conditions of bELISA,the amount of coated antigen was 200 ng per well,and dilutions of the fusion protein and tested pig sera were separately 1:320 and 1:*** cut-off value of bELISA was 24.20%,and the sensitivity and specificity were 96.43 and 92.63%,*** detecting 233 clinical pig sera with the developed bELISA and a commercial kit,the results showed that the coincidence rate of two assays was 93.99%.Additionallly,epitope mapping showed that PRV-gE-Nb36 recognized a conserved conformational epitope in different reference PRV ***,great stability and low-cost nanobody-based bELISA for detecting anti-PRV-gE antibodies were *** bELISA could be used for monitoring and eradicating PR.

关键词： nanobody nanobody-HRP blocking ELISA PRV-gE antibody

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Role of intestinal ultrasound in ulcerative colitis: A systematic review

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World Journal of Meta-Analysis 2024年第3期12卷 42-67页

作者： Partha Pal Mohammad Abdul Mateen Kanapuram Pooja Nandhakumar Rajadurai Rajesh Gupta Manu Tandan Nageshwar Reddy Duvvuru department of medical Gastroenterology Asian Institute of GastroenterologyHyderabad 500082India department of diagnostic Radiology and imaging Asian Institute of GastroenterologyHyderabad 500082India

BACKGROUND Intestinal ultrasound(IUS)is an emerging,non-invasive,and highly sensitive diagnostic tool in inflammatory bowel disease(IBD),including ulcerative colitis(UC).Despite its potential,its adoption in clinical practice is limited due to a lack of standardization and *** To perform a comprehensive scoping review based on a systematic literature review on IUS in UC to inform current *** Ninety-nine original articles about ultrasonography in UC were identified among 7608 citations searching PubMed and EMBASE databases for systematic *** IUS can be useful as an initial diagnostic strategy in patients with suspected IBD/*** UC,IUS can predict endoscopic response,histologic healing,and steroid responsiveness in acute severe *** can predict response to biologics/small molecules(as early as 2 wk).IUS correlates well with ileocolonoscopy,but IUS could miss rectal,jejunal,and upper GI lesions in suspected IBD and colon polyps or extra-intestinal manifestations in known *** is useful in special situations(children,pregnancy,and postoperative Crohn's disease).Inter-observer agreement is acceptable and trained physicians have comparable diagnostic ***-of-care ultrasound impacted management in 40%-60%of ***-held IUS has excellent agreement with conventional *** IUS is a non-invasive,highly sensitive tool in the diagnosis and monitoring of UC,offering excellent patient ***-of-care ultrasound by IBD physicians can significantly impact clinical decision-making.

关键词： Ulcerative colitis Intestinal ultrasound Inflammatory bowel disease Diagnosis Monitoring

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Clinical manifestations,diagnosis and long-term prognosis of adult autoimmune enteropathy:Experience from Peking Union medical College Hospital

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World Journal of Gastroenterology 2024年第19期30卷 2523-2537页

作者： Mu-Han Li Ge-Chong Ruan Wei-Xun Zhou Xiao-Qing Li Sheng-Yu Zhang Yang Chen Xiao-Yin Bai Hong Yang Yu-Jie Zhang Peng-Yu Zhao Ji Li Jing-Nan Li department of Gastroenterology Chinese Academy of Medical Sciences&Peking Union Medical College HospitalBeijing 100730China department of Pathology Chinese Academy of Medical Scinences&Peking Union Medical College HospitalBeijing 100730China department of Pathology Tianjin First Central HospitalNankai UniversityTianjin 300192China affairs Office Chinese Academy of Medical Sciences&Peking Union Medical College Hospital(West campus)Beijing 100032China

BACKGROUND Autoimmune enteropathy(AIE)is a rare disease whose diagnosis and long-term prognosis remain challenging,especially for adult AIE *** To improve overall understanding of this disease’s diagnosis and *** We retrospectively analyzed the clinical,endoscopic and histopathological characteristics and prognoses of 16 adult AIE patients in our tertiary medical center between 2011 and 2023,whose diagnosis was based on the 2007 diagnostic *** Diarrhea in AIE patients was characterized by secretory *** common endoscopic manifestations were edema,villous blunting and mucosal hyperemia in the duodenum and *** blunting(100%),deep crypt lymphocytic infiltration(67%),apoptotic bodies(50%),and mild intraepithelial lymphocytosis(69%)were observed in the duodenal ***,there were other remarkable abnormalities,including reduced or absent goblet cells(duodenum 94%,ileum 62%),reduced or absent Paneth cells(duodenum 94%,ileum 69%)and neutrophil infiltration(duodenum 100%,ileum 69%).Our patients also fulfilled the 2018 diagnostic criteria but did not match the 2022 diagnostic criteria due to undetectable anti-enterocyte *** patients received glucocorticoid therapy as the initial medication,of which 14/16 patients achieved a clinical response in 5(IQR:3-20)*** were administered to 9 patients with indications of steroid dependence(6/9),steroid refractory status(2/9),or intensified maintenance medication(1/9).During the median of 20.5 months of followup,2 patients died from multiple organ failure,and 1 was diagnosed with non-Hodgkin’s *** cumulative relapse-free survival rates were 62.5%,55.6%and 37.0%at 6 months,12 months and 48 months,*** Certain histopathological findings,including a decrease or disappearance of goblet and Paneth cells in intestinal biopsies,might be potential diagnostic criteria for adult *** long-term prognosis is still unsatisfac

关键词： Autoimmune enteropathy Clinical manifestations diagnostic criteria Pathological features Long-term prognosis

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Global research landscape of Peutz-Jeghers syndrome and successful endoscopic management of intestinal intussusception in patients with recurrent laparotomies

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World Journal of Gastrointestinal Surgery 2024年第8期16卷 2702-2718页

作者： Qi Sun Xiao-Ying Wang Guang-Jin Guo Lei Wang Li-Min Meng Yun-Fei Guo Tao Sun Shou-Bin Ning department of Gastroenterology Air Force Medical CenterBeijing 100142China College of Life Science Northwest UniversityXi'an 710069Shaanxi ProvinceChina Clinical medical Laboratory Air Force Medical CenterBeijing 100142China department of medical imaging Air Force Medical CenterBeijing 100142China department of Pathology Air Force Medical CenterBeijing 100142China

BACKGROUND Peutz-Jeghers syndrome(PJS)has brought significant physical,psychological and economic burdens on the patients and their families due to its early onset,diagnostic and therapeutic challenges and increased recurrence *** To explore the current research status and emerging hotspots of *** Studies on PJS published during 1994-2023 were gathered based on Web of Science Core ***,a case of PJS-induced intestinal intussusception,successfully treated with endoscopic methods despite three laparotomies,was *** bibliometric and visual analysis were conducted with VOSviewer,R and *** Altogether 1760 studies were identified,indicating a steady increase in the publication *** United States had the highest influence,whereas the University of Helsinki emerged as the leading institution,and Aaltonen LA from the University of Helsinki was the most prolific *** Research,Oncogene and Endoscopy were the top three journals based on *** burst direction analysis revealed that"cancer risk","management","surveillance"and"familial pancreatic cancer"were the potential hotspots for ***,"early detection","capsule endoscopy","clinical management","double-balloon endoscopy","familial pancreatic cancer"and"molecular genetic basis"were identified as the key clusters of co-cited *** polypectomy remained effective on resolving intestinal intussusception in patients who underwent three previous *** In the last three decades,global publications related to PJS show a steadily increasing trend in *** management is currently a research hotspot.

关键词： Peutz-Jeghers syndrome Endoscopy Management Cancer Microbiota Bibliometric

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Status of clinical applications of artificial intelligence in echocardiography

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中国医学影像技术 2024年第8期40卷 1121-1123页

作者： MA Chunyan department of Cardiovascular Ultrasound the First Hospital of China Medical UniversityShenyang 110002China Clinical medical Research Center of imaging in Liaoning Province Shenyang 110002China

Artificial intelligence(AI)technology has been increasingly used in medical field with its rapid *** is one of the best imaging methods for clinical diagnosis of heart diseases,and combining with AI could further improve its diagnostic *** the applications of AI in echocardiography remained at a relatively early stage,a variety of automated quantitative and analytical techniques were rapidly emerging and initially entered clinical *** status of clinical applications of AI in echocardiography were reviewed in this article.

关键词： echocardiography artificial intelligence

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Preoperative prediction of lymphovascular and perineural invasion in gastric cancer using spectral computed tomography imaging and machine learning

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World Journal of Gastroenterology 2024年第6期30卷 542-555页

作者： Hui-Ting Ge Jian-Wu Chen Li-Li Wang Tian-Xiu Zou Bin Zheng Yuan-Fen Liu Yun-Jing Xue Wei-Wen Lin department of Radiology Fujian Medical University Union HospitalFuzhou 350001Fujian ProvinceChina Fujian Key Laboratory of Intelligent imaging and Precision Radiotherapy for Tumors Fujian Medical UniversityFuzhou 350001Fujian ProvinceChina Digestive Hematological and Breast MalignanciesClinical Research Center for Radiology and Radiotherapy of Fujian ProvinceFuzhou 350001Fujian ProvinceChina department of Radiation Oncology Fujian Medical University Union HospitalFuzhou 350001Fujian ProvinceChina department of diagnostic Radiology Fujian Medical University Union HospitalFuzhou 350001Fujian ProvinceChina School of Electrical and Computer Engineering University of OklahomaOklahomaOK 73019United States

BACKGROUND Lymphovascular invasion(LVI)and perineural invasion(PNI)are important prognostic factors for gastric cancer(GC)that indicate an increased risk of metastasis and poor *** preoperative prediction of LVI/PNI status could help clinicians identify high-risk patients and guide treatment ***,prior models using conventional computed tomography(CT)images to predict LVI or PNI separately have had limited *** CT provides quantitative enhancement parameters that may better capture tumor *** hypothesized that a predictive model combining clinical and spectral CT parameters would accurately preoperatively predict LVI/PNI status in GC *** To develop and test a machine learning model that fuses spectral CT parameters and clinical indicators to predict LVI/PNI status *** This study used a retrospective dataset involving 257 GC patients(training cohort,n=172;validation cohort,n=85).First,several clinical indicators,including serum tumor markers,CT-TN stages and CT-detected extramural vein invasion(CT-EMVI),were extracted,as were quantitative spectral CT parameters from the delineated tumor ***,a two-step feature selection approach using correlation-based methods and information gain ranking inside a 10-fold cross-validation loop was utilized to select informative clinical and spectral CT parameters.A logistic regression(LR)-based nomogram model was subsequently constructed to predict LVI/PNI status,and its performance was evaluated using the area under the receiver operating characteristic curve(AUC).RESULTS In both the training and validation cohorts,CT T3-4 stage,CT-N positive status,and CT-EMVI positive status are more prevalent in the LVI/PNI-positive group and these differences are statistically significant(P<0.05).LR analysis of the training group showed preoperative CT-T stage,CT-EMVI,single-energy CT values of 70 keV of venous phase(VP-70 keV),and the ratio of standardized iodine co

关键词： Spectral computed tomography Gastric cancer Lymphovascular invasion Perineural invasion

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Contribution and Relevance of Transfontanellar Ultrasound in Newborns in Ouagadougou: Multicentre Study on 1000 Cases

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Open Journal of Radiology 2024年第2期14卷 64-73页

作者： Ouedraogo Pakisba Ali Ouedraogo Nina-Astrid Somé Milckisédek Judickael Dabire Stive Arnauld Tankoano Ida Aida Napon Aischa Madina Nikiema Zakari Diallo Ousseini Lougue Sorgho Leonie Claudine Cisse Rabiou department of medical imaging Ouahigouya Regional University Hospital Centre Ouahigouya University Ouahigouya Burkina Faso department of diagnostic and Interventional Radiology Bogodogo University Hospital Ouagadougou Burkina Faso department of medical imaging Souro Sanon University Hospital Bobo Dioulasso Burkina Faso medical imaging Service Yalgado Oudraogo University Hospital Ouagadougou Burkina Faso department of medical imaging Charles de Gaulle Pediatric University Hospital Ouagadougou Burkina Faso Radiodiagnostic and medical imaging Laboratory Health Sciences Training and Research Unit Joseph Ki-Zerbo University Ouagadougou Burkina Faso

Objectives: To demonstrate the contribution and relevance of ETFs through the study of 1000 examination reports carried out in the medical imaging departments of the OUAGADOUGOU CHU. Material and method: Analytical descriptive study with retrospective collection, extended from 1st January 2020 to 1st January 2022. Results: Of the 1000 transfontanellar ultrasound reports, the mean age of patients was 7.61 +/ 7.5 days, with extremes of zero and 28 days. Sex was specified in 989 cases. Males accounted for 54.49% and females for 45.51%. 555 transfontanellar ultrasound were performed in 2020. 441 in 2021 and 4 in 2022. 61.9% of transfontanellar ultrasound were performed at the Bogodogo University Hospital, 205 at Charles de Gaulle and 176 at Tengandogo. Indications for transfontanellar ultrasound were dominated by neonatal distress (65.8%), followed by convulsions (10.2%) and prematurity (9.1%). Transfontanellar ultrasound was normal in 570 cases (57%) and abnormal in 430 cases (43%). Abnormalities were dominated by haemorrhage and ischaemic lesions in 66.28% (285) and 21.63% (93) of cases respectively. In the group of normal transfontanellar ultrasound, neonatal distress represented 59.65% of indications and prematurity 10.7% of indications. As for abnormal transfontanellar ultrasound, neonatal suffering accounted for 73.95% of indications and convulsions for 12.56%. The average age ofpatients with an abnormal transfontanellar ultrasound was 8.74 days +/ 7.89 days. The indication for investigations was relevant in 42.2% of cases and irrelevant in 57.8%;of the transfontanellar ultrasound with relevant indications, 0.71 were normal and 99.29 abnormal;of the transfontanellar ultrasound with irrelevant indications, the transfontanellar ultrasound was normal in 98.1% and abnormal in 1.9%. Conclusion: Transfontanellar ultrasound is an important part of ultrasound in current practice. Haemorrhage, anoxic-ischaemic lesions and hydrocephalus are the most frequent pathologies fou

关键词： Transfontanellar Ultrasound Neonatal Distress Relevance

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Autoantibodies related to ataxia and other central nervous system manifestations of gluten enteropathy

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World Journal of Clinical Cases 2024年第12期12卷 2031-2039页

作者： Tsvetelina Velikova Georgi Vasilev Russka Shumnalieva Lyubomir Chervenkov Dimitrina Georgieva Miteva Milena Gulinac Stamatios Priftis Snezhina Lazova medical Faculty Sofia University"St.Kliment Ohridski"Sofia 1407Bulgaria Clinic of Neurology and department of Emergency Medicine UMHAT"Sv.Georgi"Plovdiv 4000Bulgaria department of Rheumatology Clinic of RheumatologyUniversity Hospital"St.Ivan Rilski"Medical University-SofiaSofia 1612Bulgaria department of diagnostic imaging Medical University PlovdivPlovdiv 4000Bulgaria department of Genetics Faculty of BiologySofia University"St.Kliment Ohridski"Sofia 1164Bulgaria department of General and Clinical Pathology Medical University of PlovdivPlovdiv 4002Bulgaria department of Healthcare Faculty of Public Health“Prof.Tsekomir VodenicharovMDDSc”Medical University of SofiaSofia 1407Bulgaria department of Pediatric University Hospital"N.I.Pirogov"Sofia 1606Bulgaria

Gluten ataxia and other central nervous system disorders could be linked to gluten enteropathy and related *** this narrative review,we focus on the various neuro-logical manifestations in patients with gluten sensitivity/celiac disease,immunological and autoimmune mechanisms of ataxia in connection to gluten sensitivity and the autoantibodies that could be used as a biomarker for diagnosing and *** focused on the anti-gliadin antibodies,antibodies to different isoforms of tissue transglutaminase(TG)(anti-TG2,3,and 6 antibodies),anti-glycine receptor antibodies,anti-glutamine acid decarboxylase antibodies,anti-deamidated gliadin peptides antibodies,*** studies found a higher prevalence of these antibodies in patients with gluten sensitivity and neurological dysfunction,presented as different neurological *** also discuss the role of a gluten-free diet on the clinical improvement of patients and also on imaging of these disorders.

关键词： Gluten ataxia Celiac disease Gluten enteropathy Autoantibodies Anti-gliadin antibodies Anti-bodies to tissue transglutaminase Anti-tissue transglutaminase antibodies Anti-transglutaminase 6 antibodies Anti-glycine receptor antibodies Anti-glutamine acid decarboxylase antibodies

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CT Thresholds for Spinal Bone Mineral Attenuation to Prioritize Eligible Older Women for Osteoporosis Screening

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Open Journal of Radiology 2024年第2期14卷 92-101页

作者： Seo Yeon K. Orite Bryn Higuchi Hyo-Chun Yoon Lana H. Gimber St. Elizabeths medical Center Brighton MA USA University of California Irvine CA USA Kaiser Permanente Division of Research Oakland CA USA department of diagnostic imaging Hawaii Permanente Medical Group Honolulu HI USA

Introduction: Computed tomography (CT) measurements of bone mineral attenuation may be a useful means to identify older women who should be prioritized for bone mineral density screening. Methods: We compared bone mineral attenuation as measured in the L1 vertebra of CT studies to the results of dual-energy x-ray absorptiometry (DEXA) studies to determine what CT attenuation thresholds might yield a reasonable combination of sensitivity and specificity for the detection of osteoporosis. The study was limited to women between the ages of 65 and 75 years who had a DEXA study and a CT that included the L1 or adjacent vertebra performed within 3 years of the DEXA study. Results: There were 1226 women in this study, of whom 452 (38%) had osteoporosis based on a T-score ≤ −2.5 by DEXA. There were 830 CT studies performed with contrast and 396 studies which were performed without contrast. There was a statistically significant difference in the mean HU of those studies performed without contrast compared to those with contrast (unenhanced mean 103 HU versus 125 HU, p < 0.001). Different CT attenuation thresholds provided the most appropriate combination of sensitivity and specificity for the detection of osteoporosis when comparing CT studies performed without or with IV contrast and when all the CT data were used in aggregate. Conclusion: Different thresholds appear necessary when using the mean CT vertebral attenuation to identify older women for preferential referral for DEXA studies.

关键词： Osteoporosis Bone Mineral Attenuation CT Threshold

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MRI Findings and diagnostic Significance of Soft Tissue Schwannomas in the Extremities

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Journal of Clinical and Nursing Research 2024年第8期8卷 31-36页

作者： Guoping Zhang Xiaoli Chen Center of diagnostic medical imaging Shaanxi Provincial People’s HospitalXi’an 710068Shaanxi ProvinceChina department of Neurology Shaanxi Provincial People’s HospitalXi’an 710068Shaanxi ProvinceChina

Objective:To analyze the MRI features and rare manifestations of schwannomas of the extremities to improve ***:Retrospective analysis of 23 cases of schwannomas in the extremities confirmed by surgical pathology,summarizing its signs,signal characteristics and MRI ***:Among the 23 cases,20 were single lesions,and 3 were multiple lesions,resulting in a total of 28 *** distribution of these lesions included 7 in the thigh,9 in the calves,4 in the armpits,3 in the hips,and 4 each in the upper arms and ***,there was one lesion located in the *** T1-weighted imaging(T1WI),the lesions showed iso-intensity or *** T2-weighted imaging(T2WI),they appeared slightly hyperintense or *** lesions exhibited multiple small vesicle-like higher signals,cystic changes,and fiber spacing on ***,one lesion showed a liquid-liquid ***“target sign”was observed in 18 lesions(64.3%)and“neuropathic signs”were present in six lesions(21.4%).Finally,it was noted that on MRI enhancement,the lesions demonstrated uniform or uneven *** lesions were uniformly or unevenly enhanced on MRI ***:The MRI findings of schwannoma in the extremities exhibit specific *** cystic changes on T2WI also display distinct *** of rare MRI findings,such as cystic changes,liquid-liquid plane and septum,should be emphasized to enhance the accuracy of pre-diagnosing schwannomas.

关键词： Schwannomas Extremities Magnetic resonance imaging

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