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Immobilization of organophosphorus hydrolase enzyme by covalent attachment on modified cellulose microfibers using different chemical activation strategies:Characterization and stability studies

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Chinese Journal of Chemical Engineering 2019年第1期27卷 191-199页

作者： Meisam Sharifi Seyed-Mortaza Robatjazi Minoo Sadri Jafar Mohammadian Mosaabadi department of bioscience and biotechnology Malek Ashtar University of Technology

The plant cellulose powder was activated by two different methods using 1,4-butanediol diglycidyl ether(BTDE)and 1,1′-Carbonyldiimidazole(CDI) as the chemical coupling *** hydrolase(OPH) from Flavobacterium ATCC 27551 was immobilized on any of activated support through covalent *** optimal conditions of affecting parameters on enzyme immobilization in both methods were found, and it was demonstrated that the highest activity yields of immobilized OPH onto epoxy and CDI treated cellulose were 68.32%and 73.51%, *** surface treatment of cellulose via covalent coupling with BTDE and CDI agents was proved by FTIR *** kinetic constants of the free and immobilized enzymes were determined, and it was showed that both immobilization techniques moderately increased the Kmvalue of the free *** improvements in storage and thermal stability were investigated and depicted that the half-life of immobilized OPH over the surface of epoxy modified cellulose had a better growth compared to the free and immobilized enzymes onto CDI treated ***, the pH stability of the immobilized preparations was enhanced relative to the free counterpart and revealed that all enzyme samples would have the same optimum pH value for stability at ***, the immobilized OPH onto epoxy and CDI activated cellulose retained about 59% and 68% of their initial activity after ten turns of batch operation, *** results demonstrated the high performance of OPH enzyme in immobilized state onto an inexpensive support with the potential of industrial applications.

关键词： Organophosphorus hydrolase Cellulose powder Activation Immobilization Stability

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BCI induces apoptosis via generation of reactive oxygen species and activation of intrinsic mitochondrial pathway in H1299 lung cancer cells

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Science China(Life Sciences) 2018年第10期61卷 1243-1253页

作者： Jong-Woon Shin Sae-Bom Kwon Yesol Bak Sang-Ku Lee Do-Young Yoon department of bioscience and biotechnology Konkuk University Targeted Medicine Research Center Korea Research Institute of Bioscience and Biotechnology (KRIBB)

The compound(E)-2-benzylidene-3-(cyclohexylamino)-2,3-dihydro-1 H-inden-1-one(BCI) is known as an inhibitor of dual specific phosphatase 1/6 and mitogen-activated protein kinase. However, its precise anti-lung cancer mechanism remains unknown. In this study, the effects of BCI on the viability of non-small cell lung cancer cell lines NCI-H1299, A549, and NCI-H460 were evaluated. We confirmed that BCI significantly inhibited the viability of p53(-) NCI-H1299 cells as compared to NCI-H460 and A549 cells, which express wild-type p53. Furthermore, BCI treatment increased the level of cellular reactive oxygen species and pre-treatment of cells with N-acetylcysteine markedly attenuated BCI-mediated apoptosis of NCI-H1299 cells. BCI induced cellular morphological changes, inhibited viability, and produced reactive oxygen species in NCI-H1299 cells in a dose-dependent manner. BCI induced processing of caspase-9, caspase-3, and poly ADP-ribose polymerase as well as the release of cytochrome c from the mitochondria into the cytosol. In addition, BCI downregulated Bcl-2 expression and enhanced Bax expression in a dose-dependent manner in NCI-H1299 cells. However, BCI failed to modulate the expression of the death receptor and extrinsic factor caspase-8 and Bid, a linker between the intrinsic and extrinsic apoptotic pathways in NCI-H1299 cells. Thus, BCI induces apoptosis via generation of reactive oxygen species and activation of the intrinsic pathway in NCI-H1299 cells.

关键词： non-small cell lung cancer apoptosis BCI cytotoxicity anticancer ROS

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Remediation of polycyclic aromatic hydrocarbon-contaminated soils using microbes and nanoparticles: A review

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Pedosphere 2023年第1期33卷 93-104页

作者： Ritu PANWAR Jyoti MATHUR department of bioscience and biotechnology Banasthali VidyapithRajasthan 304022India

Polycyclic aromatic hydrocarbon(PAH)pollution is a global concern because of their toxicity to environment and ecosystem,which induces adverse effects on plants,animals,and *** are mainly released from natural and anthropogenic activities,such as incomplete fuel combustion,leakages in oil pipelines,and the extensive use of pesticides;PAH contaminants include petroleum hydrocarbons(PHCs),halogenated hydrocarbons,chlorophenols,and *** using microorganisms such as bacteria and fungi is developing as an effective and promising technique to remediate PAHs from the *** to their remediation efficiency,this method is environmentally friendly and represents a novel approach for reducing the risk of PAHs to human health and the *** may be applied in bioremediation,and will not only induce fewer toxic effects but also reduce the effective overall time and costs *** article provides a summary of the application of nanomaterials,bacteria,and fungi to PAH remediation;we review and briefly discuss the structure,properties,sources,fate,toxicity,and remediation approaches involved in the remediation of PAH-polluted sites.

关键词： bioremediation microorganisms pesticides persistent organic pollutants petroleum hydrocarbons

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Cloning, expression and biocharacterization of OfCht5, the chitinase from the insect Ostrinia furnacalis

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Insect Science 2013年第2期20卷 147-157页

作者： Qingyue Wu Tian Liu Qing Yang department of bioscience and biotechnology Dalian University of Technology Dafian China

Chitinase catalyzes β,4-glycosidic linkages in chitin and has attracted re- search interest due to it being a potential pesticide target and an enzymatic tool for preparation of N-acetyl-β D-glucosamine. An individual insect contains multiple genes encoding chitinases, which vary in domain architectures, expression patterns, physiological roles and biochemical properties. Herein, Of ChtS, the glycoside hydrolase family 18 chiti- nase from the widespread lepidopteran pest Ostrinia furnacalis, was cloned, expressed in the yeast Pichia pastoris and biochemically characterized in an attempt to facilitate both pest control and biomaterial preparation. Complementary DNA sequence analysis indicated that Of CHT5 consisted of an open reading frame of 1 665-bp nucleotides. Phy- logenic analysis suggested Of Cht5 belongs to the Group I insect chitinases. Expression of Of Cht5 in Pichia pastoris resulted in highest specific activity after 120 h of induction with methanol. Through two steps of purification, consisting of ammonium sulfate pre- cipitation and metal chelating chromatography, about 7 mg of the recombinant Of Cht5 was purified to homogeneity from 1 L culture supernatant. Of Cht5 effectively converted colloidal chitin into chitobiose, but had relatively low activity toward a-chitin. When chi- tooligosaccharides [（GlcNAc）n, n = 3-6] were used as substrates, Of Cht5 was observed to possess the highest catalytic efficiency parameter toward （GlcNAc）4 and predominan- tely hydrolyzed the second glycosidic bond from the non-reducing end. Together with fl-N-acetyl-D-hexosaminidase OfHexl, Of Cht5 achieved its highest efficiency in chitin degradation that yielded N-acetyl-β D-glucosamine, a valuable pharmacological reagent and food supplement, within a molar concentration ratio of Of Cht5 versus Of Hexl in the range of 9 ： 1-15 ： 1. This work provides an alternative to existing preparation of chitinase for pesticides and other applications.

关键词： chitin chitinase glycoside hydrolase N-acetyl-β-D-glucosamine Ostriniafurnacalis pest control

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Curcumin-induced cell death depends on the level of autophagic flux in A172 and U87MG human glioblastoma cells

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Chinese Journal of Natural Medicines 2020年第2期18卷 114-122页

作者： Lee Jong-Eun Yoon Sung Sik Lee Jae-Wook Moon Eun-Yi department of bioscience and biotechnology Sejong UniversitySeoul 05006Republic of Korea

Glioblastoma is the deadliest neoplasm with the worst 5-year survival rate among all human *** promotes autophagic cell death or blocks the induction of apoptosis in eukaryotic ***,we investigated whether varying levels of autophagic flux in glioblastoma lead to different efficacies of curcumin treatment using U87 MG and A172 human glioblastoma *** number of LC3 puncta,the number of cells with LC3 puncta and the level of LC3 Ⅱ,Atg5 and Atg7 protein were higher in U87 MG cells compared with A172 *** the cells were incubated with curcumin for 24 or 48 h,the percentage of cell death was higher in A172 cells compared with U87 MG *** the level of LC3 was lower,that of curcumin-induced LC3 was higher,in A172 cells than in U87 MG *** relative increases in cell death and LC3-mediated autophagy were greater under serum starvation in A172 cells compared with U87 MG ***-induced A172 cell death was reduced by serum *** both types of cells were transfected with LC3-GFP,the percentage of cell death was higher in A172 cells than that in U87 MG *** together,the data demonstrate that curcumin-mediated tumor cell death is regulated by the basal level of autophagic flux in different glioblastoma *** suggests that prior to the use of various curcumin therapeutics,the level of basal or induced autophagic flux should be carefully examined in tumor cells for the best efficacy.

关键词： Glioblastoma A172 U87MG Autophagic flux Curcumin

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Identification of the Regulator of G-Protein Signaling Protein Responsive to Plant Hormones and Abiotic Stresses in Brassica napus

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Journal of Integrative Agriculture 2014年第12期13卷 2634-2644页

作者： CHEN Yun ZHU Xia ZHU Xiao-bin YU Yi-fan GE Hui-min GAO Yong LIANG Jian-sheng College of bioscience and biotechnology Yangzhou University

Regulator of G protein signaling proteins （RGS） accelerate the rate of GTP hydrolysis by Gαproteins, thus acting as negative regulators of G-protein signaling. Studies on Arabidopsis and soybean have proven that RGS proteins are physiologically important in plants and contribute to the signaling pathways regulated by different stimuli. Brassica napus is an important agriculturally relevant plant, the wildly planted oilseed rape in the world, which possesses an identiifed Gα, Gβand Gγsubunits. In the present study, we identiifed and characterized a Brassica napus RGS gene, BnRGS1, which contained an open reading frame of 1 380 bp encoding a putative 52.6 kDa polypeptide of 459 amino acids, within seven putative transmembrane domains in the N-terminal and RGS box in the C-terminal. BnRGS1 is located on the membrane in onion epidermal cells and tobacco leaves, and interacts with BnGA1 in the mating-based split-ubiquitin system. The expression levels of BnRGS1 were quite different in different tissues and developmental stages, and induced by abscisic acid （ABA） and indole-3-acetic acid （IAA）. The effects of gibberellin （GA3） and brassinolide （BR） on the expression of BnRGS1 were irregular under the concentrations tested. Moreover, the transcript level of BnRGS1 was also induced by polyethylene glycol （PEG）, whereas remained little changed by 200 mmol L-1 NaCl. These results suggested that the BnRGS1 may be involved in B. napus response to plant hormone signaling and abiotic stresses.

关键词： BnRGS1 expression plant hormone abiotic stress

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Effects of maternal stress induced by predator odors during gestation on behavioral and physiological responses of offspring in Brandt’s vole(Lasiopodomys brandtii)

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Integrative Zoology 2018年第6期13卷 723-734页

作者： Chen GU Wenjia WANG Xiaoqian DING Shengmei YANG Aiqin WANG Baofa YIN Wanhong WEI department of Animal Behavior College of Bioscience and BiotechnologyYangzhou UniversityYangzhouChina

Maternal effects play a crucial role in regulating populations of small mammals and anti-predator behaviors of *** present study investigated the effects of maternal stress induced by exposure to predator odors during gestation on the behavioral and physiological responses of offspring in Brandt’s vole(Lasiopodomys brandtii).Effects included changes in growth,behaviors,hormone levels in serum,and fosB/ΔfosB mRNA and FosB/ΔFosB protein expression in the *** results showed that when pregnant voles were exposed to cat and rabbit urine odors for 18 days(1 h/day),the weight of the female offspring decreased;however,the thymus and ovary indices increased,compared with the control *** offspring of voles exposed to cat odor spent more time exploring and foraging,and showed a decrease in corticosterone(CORT)compared with voles in the control *** fosB/ΔfosB mRNA and FosB/ΔFosB protein expressions were downregulated in the hypothalamus of male offspring in the cat odor-exposed group compared to that in offspring in the control ***,the mRNA levels of fosB/ΔfosB in the hypothalamus of female offspring did not decrease in response to cat urine *** findings suggested that maternal stress induced by predator odors during gestation restrained the growth of female offspring and increased the duration of exploring and foraging behaviors of male offspring by regulating the HPA axis,which,in turn,reduced the sensitivity of offspring to unfamiliar environments and increased their probability of becoming prey.

关键词： behaviors Brandt’s voles cat urine odor FosB/ΔFosB maternal effect

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Chlorogenic Acid Maintains Glucose Homeostasis through Modulating the Expression of SGLT-1,GLUT-2,and PLG in Different Intestinal Segments of Sprague-Dawley Rats Fed a High-Fat Diet

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Biomedical and Environmental Sciences 2015年第12期28卷 894-903页

作者： PENG Bing Jie ZHU Qi ZHONG Ying Li XU Shi Hao WANG Zheng College of bioscience and biotechnology Hunan Agricultural University

Objective To reveal the effects and related mechanisms of chlorogenic acid(CGA)on intestinal glucose *** Forty male Sprague-Dawley rats were randomly and equally divided into four groups:normal chow(NC),high-fat diet(HFD),HFD with low-dose CGA(20 mg/kg,HFD-LC),and HFD with high-dose CGA(90 mg/kg,HFD-HC).The oral glucose tolerance test was performed,and fast serum insulin(FSI)was detected using an enzyme-linked immunosorbent *** m RNA expression levels of glucose transporters(Sglt-1 and Glut-2)and proglucagon(Plg)in different intestinal segments(the duodenum,jejunum,ileum,and colon)were analyzed using quantitative real-time polymerase chain ***-1 protein and the morphology of epithelial cells in the duodenum and jejunum was localized by using *** At both doses,CGA ameliorated the HFD-induced body weight gain,maintained FSI,and increased postprandial 30-min glucagon-like peptide 1 ***-dose CGA inhibited the HFD-induced elevation in Sglt-1 *** CGA doses normalized the HFD-induced downregulation of Glut-2 and elevated the expression of Plg in all four intestinal *** An HFD can cause a glucose metabolism disorder in the rat intestine and affect body glucose *** can modify intestinal glucose metabolism by regulating the expression of intestinal glucose transporters and Plg,thereby controlling the levels of blood glucose and insulin to maintain glucose homeostasis.

关键词： Chlorogenic acid High-fat diet Intestine Glucose homeostasis SGLT-1 GLUT-2 PLG GLP-1

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Therapeutic targeting of cellular prion protein: toward the development of dual mechanism anti-prion compounds

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Neural Regeneration Research 2025年第4期20卷 1009-1014页

作者： Antonio Masone Chiara Zucchelli Enrico Caruso Giovanna Musco Roberto Chiesa Laboratory of Prion Neurobiology Department of NeuroscienceIstituto di Ricerche Farmacologiche Mario Negri IRCCSMilanItaly Biomolecular NMR Unit Division of Genetics and Cell BiologyIRCCS Ospedale San RaffaeleMilanItaly department of biotechnology and Life Sciences University of InsubriaVareseItaly

PrPSc,a misfolded,aggregation-prone isoform of the cellular prion protein(PrPC),is the infectious prion agent responsible for fatal neurodegenerative diseases of humans and other *** adopt different pathogenic conformations(prion strains),which can be resistant to potential drugs,or acquire drug resistance,posing challenges for the development of effective *** PrPCis the obligate precursor of any prion strain and serves as the mediator of prion neurotoxicity,it represents an attractive therapeutic target fo r prion *** this minireview,we briefly outline the approaches to target PrPCand discuss our recent identification of Zn(Ⅱ)-Bn PyP,a PrPC-targeting porphyrin with an unprecedented bimodal mechanism of *** argue that in-depth understanding of the molecular mechanism by which Zn(Ⅱ)-Bn PyP targets PrPCmay lead toward the development of a new class of dual mechanism anti-prion compounds.

关键词： anti-prion drug anti-PrPC antibody antisense oligonucleotide neurodegeneration pharmacological chaperone porphyrin prion disease PrPC degrader PrPC shedding zinc finger repressor

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Rapid progress of DNA replication studies in Archaea,the third domain of life

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Science China(Life Sciences) 2012年第5期55卷 386-403页

作者： ISHINO Yoshizumi ISHINO Sonoko department of bioscience and biotechnology Graduate School of Bioresource and Bioenvironmental Sciences Kyushu University

Archaea,the third domain of life,are interesting organisms to study from the aspects of molecular and evolutionary *** cells have a unicellular ultrastructure without a nucleus,resembling bacterial cells,but the proteins involved in genetic information processing pathways,including DNA replication,transcription,and translation,share strong similarities with those of ***,archaea provide useful model systems to understand the more complex mechanisms of genetic information processing in eukaryotic ***,the hyperthermophilic archaea provide very stable proteins,which are especially useful for the isolation of replisomal multicomplexes,to analyze their structures and *** review focuses on the history,current status,and future directions of archaeal DNA replication studies.

关键词： Archaea DNA replication DNA polymerase replication fork replisome hyperthermophile

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