检索结果-南通市图书馆

Qualitative Evaluation of the Capacity of LactobacillusStrains to degrade Mycotoxins developed and Accumulated by Strains of the Genus Alternaria

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Food and Nutrition Sciences 2013年第7期4卷 28-30页

作者： L. Pérez G. Girmé E. L. Arosemena M. A. Calvo Torras Grupo de Investigación en Microbiología Aplicada y Medio-Ambiental Departamento de Sanidad y de Anatomia Animales Fa- cultad de Veterinaria Universidad Autónoma de Barcelona Bellaterra Spain.

Mycotoxins are secondary metabolites produced and accumulated by mould. This study assessed the ability of 89 lactic acid bacteria, isolated from different sources, to degrade two mycotoxins by Alternaria: alternariol and altenuene. The mehodology employed was the thin layer chromatography. The results obtained allow us to demonstrate that a 16.85% of the strains (15/89 strains) are capable of decreasing the intensity of the band related at the alternariol and 14.60% (13/89 strains) is capable of decrease the intensity of the band that manifests the altenuane in Alufolium. All tests have been observed under wavelengths of 360 nm and 255 nm. The results obtained allow carrying out a selection of lactic acid strains capable of degrading or decreasing the concentration of mycotoxins selected for this.

关键词： Lactic Acid Bacteria Mycotoxins Alternaria Thin Layer Chromatography

Gene expression changes in spleens of the wildlife reservoir species,Eurasian wild boar(Sus scrofa),naturally infected with Brucella suis biovar 2

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2010年第11期37卷 725-736页

作者： Ruth C.Galindo Pilar M.Muoz María J.de Miguel Clara M.Marin Javier Labairu Miguel Revilla José M.Blasco Christian Gortazar José de la Fuente Instituto de Investigación en Recursos Cinegéticos IREC(CSIC-UCLM-JCCM) Unidad de sanidad Animal Centro de Investigación y Tecnología Agroalimentaria(CITA) ITG Ganadero Avda. Serapio Huici 2231610 VillabaNavarraSpain departamento de Patología Animal Facultad de VeterinariaUniversity of Zaragoza department of Veterinary Pathobiology Center for Veterinary Health SciencesOklahoma State University

Brucella suis is responsible for swine brucellosis worldwide. Of the five different B. suis biovars （by.）, bv. 2 appears restricted to Europe where it is frequently isolated from wild boar and hares, can infect pigs and can cause human brucellosis. In this study, the differ- ential gene expression profile was characterized in spleens of Eurasian wild boar naturally infected with B. suis bv. 2. Of the 20,201 genes analyzed in the microarray, 633 and 1,373 were significantly （fold change 〉 1.8; P 〈 0.01） upregulated and downregulated, respectively, in infected wild boar. The analysis was focused on genes that were over represented after conditional test for biological process gene ontology. Upregulated genes suggested that B. suis bv. 2 infection induced cell maturation, migration and/or proliferation in infected animals. The genes downregulated in infected wild boar impaired the activity of several important cellular metabolic pathways such as metabolism, cytoskeleton organization and biogenesis, immune response and lysosomal function and vesicle-mediated transport. In addition, the response to stress, sperm fertility, muscle development and apoptosis seemed to be also impaired in infected animals. These results suggested that B. suis bv. 2 may use strategies similar to other smooth brucellae to facilitate intracellular multiplication and the development of chronic infections. To our knowledge, this is the first report of the analysis of gene expression profile in hosts infected with B. suis bv. 2, which is important to understand the molecular mechanisms at the host-pathogen interface in the main reservoir species with possible implications in the zoonotic cycle of the pathogen.

关键词： Brucella suis boar genomics microarray systems biology

Neutralization of Omicron BA.1,BA.5.1.6,BQ.1.3 and XBB1.1 induced by heterologous vaccination Ad5-nCoV and mRNA-1273

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2023年第5期8卷 2085-2087页

作者： Jesús Hernández Freddy dehesa-Canseco Alma B.Vázquez-López Mónica Reséndiz-Sandoval Graciela Caire-Juvera Mario Solís-Hernández Olivia Valenzuela Bruno Gómez-Gil Verónica Mata-Haro Centro de Investigación en Alimentación y desarrollo A.C.HermosilloSonora83304Mexico Comisión México-Estados Unidos para la Prevención de la Fiebre Aftosa y otras Enfermedades Exóticas de los animales(CPA) SENASICASADERCiudad de México05010Mexico departamento de Ciencias Químico Biológicas Universidad de SonoraHermosilloSonora83000Mexico

dear Editor,The emergence of Omicron subvariants has required additional boosters to induce a highly neutralizing antibody response.1 However,the new Omicron subvariants BQ.1 and XBB,evade the humoral response induced by multiple mRNA vaccinations and infections.2,3 CansinoBio(Ad5-nCoV)is a single-dose adenovirus vector-based vaccine with high protection against severe disease4 but lower efficacy and antibody response than mRNA ***,hybrid immunity induced by infection plus vaccine or heterologous vaccines has produced a robust neutralizing antibody response.

关键词： vaccination vaccine immunity

Molecular Study of Rickettsiae in Serum Cattle

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Open Journal of Preventive Medicine 2018年第8期8卷 260-265页

作者： Lourdes Lledó Consuelo Giménez-Pardo Rufino álamo departamento de Biomedicina y Biotecnología Universidad Alcalá Alcalá de Henares Spain Consejería de sanidad y Bienestar Social de la Junta de Castilla y León Valladolid Spain

A molecular survey of 230 serum samples from cattle was studied by PCR-amplification of the citrate synthase gene gltA, the gene coding for protein 190 kDa—ompA—and the gene ompB. The study was carried out in the Junta of Castilla y León (northern Spain). The results suggest that the molecular study of the serum cattle would not make a good method in epidemiological studies on rickettsiae in this region. But it is necessary to continue and expand the work with more sensitive molecular methods.

关键词： Rickettsiae Epidemiology Molecular Assay

Epidemiological Aspects and Clinicopathological Findings in Cats Naturally Infected with Feline Leukemia Virus (FeLV) and/or Feline Immunodeficiency Virus (FIV)

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Open Journal of Veterinary Medicine 2012年第1期2卷 13-20页

作者： Victorio M. Collado Ana Domenech Guadalupe Miró Sonsoles Martin Elena Escolar Esperanza Gomez-Lucia departamento Medicina y Cirugía Animal Facultad de Veterinaria Universidad Complutense Madrid Spain departamento sanidad Animal Facultad de Veterinaria Universidad Complutense Madrid Spain

Infections produced by feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV), two of the most prevalent pathogens in cats, range from passing unnoticed to presenting a wide variety of clinical signs. Different epidemiological, clinical, hematological and virological parameters were analyzed in 78 FIV- and/or FeLV-infected cats. FeLV-infected (FeLV+) cats were considerably younger than FIV-infected (FIV+) cats, and in general were seen to have a more severe disease than FIV+ cats. Around one third of the cats presented anemia, and neutropenia was also frequently observed. Though a higher percentage of FIV+ than FeLV+ cats had altered leukocyte counts, FeLV+ cats had altered counts of both neutrophils and lymphocytes more frequently than FIV+, which usually presented only either altered neutrophils or lymphocyte counts. Virological markers were only detected in FeLV+cats, either as mono- or dual-infection, and correlated with the severity of the disease, but not in FIV+ cats. In conclusion, these results suggest that FeLV affects more blood cell types and provokes death of affected animals at a much earlier age than FIV and that the severity of the disease seemed to depend on the viral status of the cat.

关键词： FIV FeLV Epidemiology Hemogram CD4:CD8 Ratio

Preliminary study of natural reservoirs as sentinels of Anaplasma phagocytophilumand Ehrlichia chaffeensisin Soria, northern Spain

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Open Journal of Preventive Medicine 2013年第9期3卷 501-503页

作者： Lourdes Lledó Consuelo Giménez-Pardo José Luis Serrano Consejería de sanidad y Bienestar Social de la Junta de Castilla y León Soria Spain departamento de Biomedicina y Biotecnología Universidad Alcalá Alcalá de Henares Spain

The serum of foxes and red deer from the Province of Soria (northern Spain) was screened in indirect immunofluorescence assays to determine whether these animals could be used as sentinels of the tick-borne pathogens Anaplasma phagocytophilum and Ehrlichia chaffeensis. The results suggest that foxes and red deer would not make good sentinels in epidemiological studies on E. chaffeensis in this region, although red deer could be used as such for the study of A. phagocytophilum transmission.

关键词： Anaplasma phagocytophilum Ehrlichia chaffeensis Foxes Red deer Sentinels Spain

First Characterization of Escherichia coliStrains Isolated from Wildlife Griffon Vulture (Gyps fulvus) in the Southeast of Spain

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Open Journal of Veterinary Medicine 2014年第12期4卷 329-333页

作者： Azucena Mora Nieves Ortega Elena Garcia Susana Viso Mónica G. Candela Ghizlane Dahbi Francisco Cuello María Rosa Caro Laboratorio de Referencia de E. coli (LREC) Departamento de Microbiología y Parasitología Facultad de Veterinaria Universidad de Santiago de Compostela Lugo Spain departamento de sanidad Animal Facultad de Veterinaria Campus de Espinardo Universidad de Murcia Murcia Spain

The aim of the present study was to characterize a collection of Escherichia coli strains isolated from asymptomatic griffon vulture (Gyps fulvus) during a reintroduction program in the southeast of Spain, in order to establish if griffon vulture could play a role in the spread of resistant or potentially pathogenic E. coli strains. For this purpose, 14 E. coli strains obtained from 10 griffon vulture were studied to establish their serotypes, phylogroups, virulencegene profiles and antimicrobial resistances. High heterogeneity was observed within the 14 strains isolated which belonged to three phylogroups (A, B1 and D), 8 serogroups (O2, O21, O29, O60, 073, O78, O103 and O141) and 13 different serotypes. Out of 34 genes screened, we have detected eight virulence genes that are typical of extraintestinal pathogenic E. coli (ExPEC) (fimH, fimAvMT78, iroN, iucD, cvaC, iss, traT and tsh);however, none of the studied strains showed the ExPEC status. The 14 strains were also analyzed for the production of extended-spectrum beta-lactamases (ESBLs) and for antimicrobial resistances. None of the 14 strains were ESBL-producing E. coli, but high resistance-prevalences to ampicillin and cotrimoxazole were detected. To our knowledge, this is the first characterization of E. coli strains isolated from griffon vulture and although they did not show high virulencegene scores, they showed cotrimoxazole resistance.

关键词： Antibiotic Resistance APEC Escherichia coli ExPEC Griffon Vulture

Biological Control of Ceratitis capitata(Diptera: Tephritidae) in Argentina: Releases of Diachasmimorpha longicaudata(Hymenoptera: Braconidae) in Fruit-Producing Semi-Arid Areas of San Juan

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Natural Science 2014年第9期6卷 664-675页

作者： Lorena Suarez Fernando Murua Natalia Lara Jorge Escobar Gustavo Taret José Luis Rubio Guido Van Nieuwenhove Laura Bezdjian Pablo Schliserman Sergio Marcelo Ovruski Laboratorio de Investigaciones Ecoetológicas de Moscas de la Fruta y sus Enemigos Naturales (LIEMEN) División Control Biológico de Plagas Planta Piloto de Procesos Industriales Microbiológicos y Biotecnología (PROIMI)—CCT Tucumán—CONICET Tucumán Argentina Programa de Control y Erradicación de Mosca de los Frutos de San Juan (ProCEM)—Dirección de sanidad Vegetal Animal y Alimentos San Juan Argentina Programa de Control y Erradicación de Mosca de los Frutos de San Juan (ProCEM)—Dirección de sanidad Vegetal Animal y Alimentos San Juan Argentina IMCN-Diversidad de Invertebrados Departamento de Biología San Juan Argentina

The Mediterranean fruit fly (Medfly), Ceratitis capitata (Wiedemann) is one of the major pests of fruit crops in Argentina and it is a phytosanitary barrier to the export of fresh fruits. In the Province of San Juan, located in the central-eastern region of Argentina known as Cuyo, control strategies against Medfly in fruit-producing irrigated-valleys have been implemented by the National Fruit Fly Control and Eradication Program (ProCEM) jointly with the provincial government and the producers. This program uses an area-wide integrated pest management approach that includes the use of environment-friendly strategies to suppress or eradicate Medfly, such as the use of the sterile insect technique and the application of new-generation bait sprays, and more recently the release of the Indo-Pacific parasitoid Diachasmimorpha longicaudata (Ashmead). This exotic larval-prepupal endoparasitoid is being mass-reared on larvae of Vienna 8 TSL C. capitata strain at the BioPlanta San Juan facility. In this respect, the objective of this study was to assess the effectiveness of laboratory-reared D. longicaudata females in order to find and successfully parasitize Medfly larvae in different host fruit species once released under semi-arid environmental conditions in ecologically isolated fruit-growing valleys of San Juan. Ground releases were used to disperse parasitoids in 6 fruit-producing valleys. In total, 40,000 adult parasitoids were released at places with various Medfly host plants bearing fruits and in which no insecticides were regularly applied, such as backyards and small orchards. 119 D. longicaudata adults were recovered from 6 Medfly-infested fruit species that were collected in 5 release sites (Pocito, Zonda, Santa Lucía, Caucete, and Rivadavia). The highest number of parasitoids was recovered from fig, followed by grape, rose, orange, tangerine, and persimmon. Data provided from this study open up the possibility of implementing a parasitoid mass-release program

关键词： Environment-Friendly Strategies Medfly Parasitoid Release Irrigated Valleys

Acceptance of living liver donation among medical students: A multicenter stratified study from Spain

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2016年第25期22卷 5800-5813页

作者： Antonio Ríos Ana Isabel López-Navas Ana Isabel López-López Francisco Javier Gómez Jorge Iriarte Rafael Herruzo Gerardo Blanco Francisco Javier Llorca Angel Asunsolo Pilar Sánchez-Gallegos Pedro Ramón Gutiérrez Ana Fernández María Teresa de Jesús Laura Martínez-Alarcón Alberto Lana Lorena Fuentes Juan Ramón Hernández Julio Virseda José yelamos José Antonio Bondía Antonio Miguel Hernández Marco Antonio Ayala Pablo Ramírez Pascual Parrilla International Collaborative Donor Project ('Proyecto Colaborativo Internacional Donante' 30007 Casillas Murcia Spain department of Surgery Paediatrics Obstetrics and Gynaecology University of Murcia Avenida de las Fuerzas Armadas 30800 Espinardo Murcia Spain Transplant Unit Surgery Service IMIB - Virgen de la Arrixaca University Hospital Carretera Madrid-Cartagena s/n 30120 El Palmar Murcia Spain Regional Transplant Centre Consejeria de Sanidad y Consumo de la Region de Murcia 30008 Ronda de Levante Murcia Spain Hospital Clinico Universitario Virgen de la Arrixaca Departamento de Cirugia General y del Aparato Digestivo Unidad de Trasplantes 30007 Casillas Murcia Spain department of Psychology Universidad Catolica San Antonio (UCAM) Avenida de los Jeronimos 30107 Guadalupe Murcia Spain department of Urology San Juan University Hospital of Alicante 03010 Calle Pintor Baeza Alicante Spain Universidad de Granada Avenida del Hospicio s/n 18010 Granada Spain Universidad de Navarra Campus Universitario 31080 Pamplona Navarra Spain Universidad Autonoma de Madrid Ciudad Universitaria de cantoblanco 28049 Madrid Spain Servicio de Cirugia HBP y Trasplante Hepatico Complejo Hospitalario Universitario de Badajoz Hospital Infanta Cristina Avenida Elvas s/n 06006 Badajoz Spain Universidad de Cantabria Avenida los Castros s/n 39005 Santander Cantabria Spain departamento de Cirugia Ciencias Medicas y Sociales Facultad de Medicina y Ciencias de la Salud Universidad de Alcala Campus Cientifico-Tecnologico Plaza de San Diego s/n 28801 Alcala de Henares Madrid Spain Facultad de Medicina Universidad de Malaga 29071 Avenida de Cervantes Malaga Spain Servicio de Urologia (Complejo Hospitalario Universitario de Canarias CHUC) y Departamento de Cirugia (Universidad de La Laguna ULL) Calle Molinos de Agua s/n 38207 San Cristobal de La Laguna Santa Cruz de Tenerife Spain departamento de Ciencias Biomedicas Basicas Facultad de Ciencias Biomedicas Universidad Europea de

AIM: To analyze the attitude of Spanish medical students toward living liver donation (LLD) and to establish which factors have an influence on this ***: Study type: A sociological, interdisciplinary, multicenter and observational study. Study population: Medical students enrolled in Spain (n = 34000) in the university academic year 2010-2011. Sample size: A sample of 9598 students stratified by geographical area and academic year. Instrument used to measure attitude: A validated questionnaire (PCID-DVH RIOS) was self-administered and completed anonymously. Data collection procedure: Randomly selected medical schools. The questionnaire was applied to each academic year at compulsory sessions. Statistical analysis: Student´s t test, χ2 test and logistic regression ***: The completion rate was 95.7% (n = 9275). 89% (n = 8258) were in favor of related LLD, and 32% (n = 2937) supported unrelated LLD. The following variables were associated with having a more favorable attitude: (1) age (P = 0.008); (2) sex (P demic year (P y of needing a transplant oneself in the future (P de toward deceased donation (P de toward living kidney donation (P y member if one were needed (P y (P ying out activities of an altruistic nature; and (12) fear of the possible mutilation of the body after donation (P dents have a favorable attitude toward LLD.

关键词： Attitude Living liver donation Medical students Transplantation Organ donation Psychosocial variables Spain