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The Beneficial Effect of Trichoderma spp. in Seed Treatment of Four Maize (Zea mays L.) Genotypes

American Journal of Plant Sciences 2023年第6期14卷 625-637页

作者： José Luis Arispe-Vázquez Abiel Sánchez-Arizpe Daniel Alejandro Cadena-Zamudio Ma. Elizabeth Galindo-Cepeda David Heriberto Noriega-Cantú Oscar Guadalupe Barrón-Bravo Leslie Carnero-Avilés Juan Mayo-Hernández Susana Elizabeth Ramírez-Sánchez Adriana Antonio-Bautista Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias Iguala de la Independencia Guerrero México departamento de Parasitología Universidad Autónoma Agraria Antonio Narro Saltillo México Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias Altamira México Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias Delicias México Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias Tepatitlán de Morelos Jalisco México departamento de fitomejoramiento Universidad Autónoma Agraria Antonio Narro Saltillo México

Maize is the main crop for Mexicans;however, it is affected by species of fungi causing ear rot. This research aimed to evaluate the effect of T. asperellum T11, T. harzianum T1 4 y T. longibrachiatum T1 40 on some agronomic variables of four maize genotypes. The seeds of the genotypes H-515, Zapata 7, and H-507 were treated with a suspension of Trichoderma spp. to 1 × 108 spores mL-1, using a control (untreated seed), and Benomyl as chemical control. The planting was in Morelos, in a completely random block design with a factorial arrangement. The ear rot was natural. Data were obtained at the end of the crop cycle and processed in SAS 9.4®. H-515 genotype had the greatest effect on the treatment of maize seeds with Trichoderma spp. (5.562 kg);T. asperellum T11 was the strain that stood out with a mean yield of 50 ears in an area of 16 m2 of 4.904 kg, and control of 4.448 kg. Our results are an economic option for farmers to contemplate the use of Trichoderma and obtain its benefits.

关键词： Seeds Treatment Trichoderma asperellum Ear Rot Yield

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development of a Rapid and Efficient Liquid Chromatography Method for determination of Gibberellin A4 in Plant Tissue, with Solid Phase Extraction for Purification and Quantification

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American Journal of Plant Sciences 2014年第5期5卷 573-583页

作者： Julia Medrano Macías Rahim Foroughbakhch Pournavab Manuel Humberto Reyes-Valdés Adalberto Benavides-Mendoza departamento de fitomejoramiento Universidad Autónoma Agraria Antonio Narro Saltillo México departamento de Horticultura Universidad Autónoma Agraria Antonio Narro Saltillo México Facultad de Ciencias Biológicas Universidad Autónoma de Nuevo León San Nicolás de los Garza México

A new, rapid and efficient reverse phase Liquid Chromatography (RP-LC) method was developed for determination of Gibberellin A4 (GA4) in samples of flower stalk of Dasylirion cedrosanum and vegetative tissue of Epithelantha micromeris. Purification of GA4 was carried out by solid phase extraction (SPE), in Epithelantha micromeris. In the chromatography method was obtaining a retention time of 2.1 min, using Hypersil GOLD C-18 column (100 × 4.6 mm dim and size particle 5 μ), mobile phase 50/50 acetonitrile/water and a flow 1.0 ml/min. detection was carried out by a UV detector set at 205 nm, and a quantization limit of 0.4 mg/L. The obtained correlation coefficient was 0.995.

关键词： Dasyrilon cedrosanum Epithelantha micromeris Phytohormone Separation Plant Growth Regulators Reverse Phase Liquid Chromatography Solid Phase Extraction

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