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Interindividual Age-Independent Differences in Human CX43 Impact Ventricular Arrhythmic Risk

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Research 2024年第3期6卷 417-432页

作者： laura García-Mendívil María Pérez-Zabalza Antoni Oliver-Gelabert JoséMaría Vallejo-Gil Javier Fañanás-Mastral Manuel Vázquez-Sancho Javier AndréBellido-Morales Alexánder Sebastián Vaca-núñez Carlos Ballester-Cuenca Emiliano Diez laura Ordovás Esther Pueyo Biomedical Signal Interpretation and Computational Simulation group(BSICoS) Aragón Institute of Engineering ResearchUniversity of ZaragozaZaragoza 50018Spain BSICoS Instituto de Investigación Sanitaria Aragón(IISA)Zaragoza 50018Spain Centro Universitario de la defensa(CUD) Zaragoza 50090Spain. department of Cardiovascular Surgery University Hospital Miguel ServetZaragoza 50009Spain. Institute of Experimental Medicine and Biology of Cuyo(IMBECU) CONICETMendoza 5500Argentina. Fundación Agencia Aragonesa para la Investigación y el desarrollo(ARAID) Zaragoza 50018Spain. Biomedical Research networking Center in Bioengineering Biomaterials and Nanomedicine(CIBER-BBN)Zaragoza 50018Spain.

connexin 43 (CX43) is one of the major components of gap junctions, the structures responsible for the intercellular communication and transmission of the electrical impulse in the left ventricle. There is limited information on the histological changes of CX43 with age and their effect on electrophysiology, especially in humans. Here, we analyzed left ventricular biopsies from living donors starting at midlife to characterize age-related CX43 remodeling. We assessed its quantity, degree of lateralization, and spatial heterogeneity together with fibrotic deposition. We observed no significant age-related remodeling of CX43. Only spatial heterogeneity increased slightly with age, and this increase was better explained by biological age than by chronological age. Importantly, we found that CX43 features varied considerably among individuals in our population with no relevant relationship to age or fibrosis content, in contrast to animal species. We used our experimental results to feed computational models of human ventricular electrophysiology and to assess the effects of interindividual differences in specific features of CX43 and fibrosis on conduction velocity, action potential duration, and arrhythmogenicity. We found that larger amounts of fibrosis were associated with the highest arrhythmic risk, with this risk being increased when fibrosis deposition was combined with a reduction in CX43 amount and/or with an increase in CX43 spatial heterogeneity. These mechanisms underlying high arrhythmic risk in some individuals were not associated with age in our study population. In conclusion, our data rule out CX43 remodeling as an age-related arrhythmic substrate in the population beyond midlife, but highlight its potential as a proarrhythmic factor at the individual level, especially when combined with increased fibrosis.

关键词： CX43 rhythm Independent

Sound waves and solutions:Point-of-care ultrasonography for acute kidney injury in cirrhosis

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World Journal of Critical Care Medicine 2024年第2期13卷 83-92页

作者： David Aguirre-Villarreal Mario Andrés de Jesús Leal-Villarreal Ignacio García-Juárez Eduardo R Argaiz Abhilash Koratala departamento de Gastroenterología Instituto Nacional de Ciencias Médicas y Nutrición Salvador ZubiránMexico City 14080Mexico Cardiología Instituto Nacional de Cardiología Ignacio ChávezMexico City 14080Mexico Unidad de Hepatología y Trasplante Departamento de GastroenterologíaInstituto Nacional de Ciencias Medicas y Nutricion Salvador ZubiranMexico City 14080Mexico departamento de nefrología y Metabolismo Mineral Instituto Nacional de Ciencias Médicas y Nutrición Salvador ZubiránMexico City 14080Mexico Tecnológico de Monterrey Escuela de Medicina y Ciencias de la SaludMexico City 64710Mexico department of nephrology Medical College of WisconsinMilwaukeeWI 53226United States

This article delves into the intricate challenges of acute kidney injury(AKI)in cirrhosis,a condition fraught with high morbidity and *** complexities arise from distinguishing between various causes of AKI,particularly hemodynamic AKI,in cirrhotic patients,who experience hemodynamic changes due to portal *** term"hepatocardiorenal syndrome"is introduced to encapsulate the intricate interplay among the liver,heart,and *** narrative emphasizes the often-overlooked aspect of cardiac function in AKI assessments in cirrhosis,unveiling the prevalence of cirrhotic cardiomyopathy marked by impaired diastolic *** conventional empiric approach involving volume expansion and vasopressors for hepatorenal syndrome is critically analyzed,highlighting potential risks and variable patient *** advocate for a nuanced algorithm for AKI evaluation in cirrhosis,prominently featuring point-of-care ultrasonography(POCUS).POCUS applications encompass assessing fluid tolerance,detecting venous congestion,and evaluating cardiac function.

关键词： Point-of-care ultrasonography Bedside ultrasound Cirrhosis congestion Acute kidney injury congestive nephropathy

Advanced strategies for marine antifouling based on nanomaterial-enhanced functional PDMS coatings

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nano materials Science 2024年第4期6卷 375-395页

作者： Xiaohui Shi Hao Wei Wenjun Zhou Paul E.D.Soto Rodriguez Cunguo Lin Lei Wang Zhijia Zhang School of materials Science and Chemical Engineering Harbin Engineering University Qingdao Innovation and development Center of Harbin Engineering University State Key laboratory for Marin Corrosion and Protection Luoyang Ship Material Research Institute (LSMRI) Instituto de Estudios Avanzados IUdeA Departamento de Física Universidad de La Laguna C/Astrofísico Francisco Sanchez School of Chemistry and Chemical Engineering Harbin Institute of Technology

Marine biofouling seriously affects human marine exploitation and transportation activities, to which marine antifouling(AF) coatings are considered to be the most cost-effective solution. Since the mid-20th century, human beings have dedicated their efforts on developing AF coatings with long cycle and high performance, leading to a large number of non-target organisms? distortion, death and marine environmental pollution. Polydimethylsiloxane(PDMS), is considered as one of the representative environment-friendly AF materials thanks to its non-toxic, hydrophobic, low surface energy and AF properties. However, PDMS AF coatings are prone to mechanical damage, weak adhesion strength to substrate, and poor static AF effect, which seriously restrict their use in the ocean. The rapid development of various nanomaterials provides an opportunity to enhance and improve the mechanical properties and antifouling properties of PDMS coating by embedding *** on our research background and the problems faced in our laboratory, this article presents an overview of the current progress in the fields of PDMS composite coatings enhanced by different nanomaterials, with the discussion focused on the advantages and main bottlenecks currently encountered in this field. Finally, we propose an outlook, hoping to provide fundamental guidance for the development of marine AF field.

关键词： nanomaterials Antifouling PDMS composites Environment-friendly Marine biofouling

A novel and Functional Artificial Bruch’s Membrane Made of Poly (ε-Caprolactone)/Gelatin for Retinal Pigment Epithelium Restoration

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Advances in Bioscience and Biotechnology 2024年第8期15卷 443-461页

作者： López Edith Silva-Bermudez Phaedra Gonzalez-Salinas Roberto Velasquillo Cristina Torres-Romero Abigail López-Colomé Ana María Pérez Armando Gina Prado-Prone Edgar Ramos-Martínez Iván Ramos-Martínez Quiroz-Mercado Hugo Hernández-Zimbrón Luis Fernando Instituto de Fisiologa Celular Universidad Nacional Autnoma de Mxico Ciudad de Mxico City Mxico Unidad de Ingeniería de Tejidos Terapia Celular y Medicina Regenerativa Instituto Nacional de Rehabilitación Luis Guiller-mo Ibarra Ibarra Ciudad de México México departamento de Investigacin Asociacin Para Evitar la Ceguera Ciudad de Mxico Mxico Subdireccin de Investigacin Tecnolgica Instituto Nacional de Rehabilitacin Luis Guillermo Ibarra Ibarra Ciudad de Mxico Mxico departamento de Biologa Celular y Tisular Facultad de Medicina Universidad Nacional Autnoma de Mxico Ciudad de Mxico Mxico Escuela de Ciencias Universidad Autnoma Benito Jurez de Oaxaca Oaxaca Mxico departamento de Medicina y Zootecnia de Cerdos Facultad de Medicina Veterinaria y Zootecnia Universidad Nacional utnoma de Mxico Mxico laboratorio de Investigacion Interdisciplinaria rea de Optometria Escuela Nacional de Estudios Superiores Unidad Len Universidad Nacional Autnoma de Mxico Len Guanajuato Mxico laboratorio nacional de ptica de la Visin-Centro de Investigaciones en ptica Len Guanajuato Mxico

Purpose: The primary aim of this paper is to design, create, and improve functional and artificial Bruch’s membranes (BM) using bioengineering techniques, which can be applied in the treatment of maculopathies by supporting the growth and maintenance of retinal pigment epithelium (RPE) cells, thereby potentially enabling subretinal implantation in patients. Methods: We fabricated by electrospinning ultrathin 3D nanofibrous membranes from Polycaprolactone (PCL), and different concentrations of gelatin (5%, 15% and 30%). ARPE-19 cells were seeded onto these artificial membranes. MTT assays were performed in order to evaluate ARPE-19 cell viability and cytotoxicity. IF assays were realized to observe the ARPE-19 cells onto each membrane. Ultrastructure of the modified Bruch’s membrane and ARPE-19 morphology after 25 days of culture were studied with transmission and scanning electron microscopy. To evaluate expression changes in markers of ARPE-19 (RPE65 and ZO-1) qRT-PCR assays were realized. Data from three independent experiments were pooled and expressed as the mean SD. A confidence level of P 0.05 was considered to be statistically significant. Results ARPE-19 cells grew on PCL/Gelatin membranes mainly in PCL/30% gelatin combination, which had not cytotoxic effect. RPE65/cytokeratin-18/ and actin-positive ARPE-19 cells formed a correctly orientated monolayer of polygonal cells with morphological polarity. The apical cell surfaces exhibited abundant protruding microfolds. Moreover, zones of polygonal border look as if ARPE-19 cells fused among, suggesting the presence of tight junctions. The expression of RPE65 and ZO-1 was unchanged. conclusions: PCL/30% Gelatine membranes may imitate the natural BM to such extent that they support RPE-cells and exhibited RPE-like morphology. The engineering of a human RPE monolayer with these artificial BM, emulating the in vivo retina, arouse their potential subretinal implantation in patients with wet age-related macular degene

关键词： Artificial Bruch’s Membrane Electrospinning ARPE-19 Cells Age-Related Macular degeneration

Phagocytosis: A Practical Approach for Evaluating neutrophil Function in Health and Disease

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Modern Research in Inflammation 2024年第2期13卷 9-28页

作者： Oscar Rojas-Espinosa Patricia Arce-Paredes Sergio Islas-Trujillo Luis Enrique Becerril-Villanueva María Dolores Ponce-Regalado departamento de Inmunologa Escuela Nacional de Ciencias Biolgicas Instituto Politcnico Nacional Ciudad de Mxico Mxico laboratorio de Psicoinmunologa Instituto Nacional de Psiquiatra Ramn de la Fuente Ciudad de Mxico Mxico departamento de Ciencias de la Salud Centro Universitario de los Altos Universidad de Guadalajara Jalisco Mxico

neutrophils, crucial players in the effector phase of the immune response, are recognized as important mediators of both innate and adaptive immune responses. Through the production of pro- and anti-inflammatory cytokines, they modulate the function of T and other lymphoid cells. Countless reports have highlighted the importance of these cells as efficient antimicrobial agents and annotated their involvement in the pathology of infectious and noninfectious diseases. The development of modern, sophisticated technologies has allowed the study of the functions of these cells in clinical settings. These advanced technologies include fluorescence-activated cell sorters, confocal microscopy, automated cell image analyzers, and live cell analysis instruments. Unfortunately, the cost of these modern instruments, maintenance, reagents, and the need for qualified technicians prohibit their use in low-income laboratories and universities in developing countries. With this in mind, we propose a series of basic tests that can be used in low-input clinical laboratories and universities to evaluate the function of neutrophils in health and disease. Our methodology allows us to assess in a practical and low-cost manner the functions of neutrophils in the phagocytic process, including opsonization, ingestion, ROI production (nBT reduction), myeloperoxidase content, phagosome-lysosome fusion, microbicidal activity, and nET production. Thus, under a disadvantageous ambiance, this may guide physicians in deciding whether a patient’s illness involves phagocytic defects without imposing a heavy financial *** Abstract[-rId13-]

关键词： Phagocytosis neutrophils Microbicidal Activity netosis

A review on the parasitic isopod genus notophryxus ***,1883(Crustacea:Isopoda),and first report of notophryxus globularis ***,1885 from lakshadweep Sea(Amini Island)

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Acta Oceanologica Sinica 2024年第8期43卷 142-153页

作者： Mukkattu nazar Suhaana Jaime Gómez-Gutiérrez Paravanparambil Rajakumar Jayachandran Punnakkal Hari Praved Sivasankaran Bijoy nandan department of Marine Biology Microbiology and BiochemistrySchool of Marine SciencesCochin University of Science and TechnologyKochi-Kerala 682016India departamento de Plancton y Ecología Marina Centro Interdisciplinario de Ciencias MarinasInstituto Politécnico NacionalLa Paz B.C.S.23096Mexico Applied Research Centre for Environment and Marine Studies King Fahd University of Petroleum and MineralsDhahran 31261Saudi Arabia

Isopod crustaceans of the family Dajidae are exclusively marine *** genus notophryxus ***,1883 currently includes nine nominal species,which are very rarely reported as the chances for encountering these specimens are *** comprehension of the taxonomy,biology,ecology,and life cycle of species belonging to the notophryxus genus is notably *** nominal species of notophryxus genus are reviewed here to obtain a comprehensive and integrative taxonomic understanding of this genus,in an attempt to address the *** paper also documents the rediscovery of an ectoparasitic isopod from the genus notophryxus in Indian waters,after 55 *** isopod specimens obtained from the outer reefs of Amini Island in the lakshadweep archipelago,Southeastern Arabian Sea,display morphological congruence to notophryxus globularis ***,*** only previous report on this species dates back to 1885 from ***'description of *** as an ectoparasite on the euphausiid Thysanoessa gregaria ***,1883 in the north *** discovery represents a range extension of *** from the north Pacific to the Arabian *** cryptoniscid larvae and three adult females of ***(with dwarf males)were found attached to four adult mysid specimens of Siriella aequiremis Hansen,*** present study provides an improved diagnosis of *** with the aid of light microscopy images and line drawings which was not given in the original ***,1885 *** research also provides a concise description of cryptoniscid larvae from the same sampling location and same host which is most likely to be *** larvae.

关键词： Dajiidae Mysid cryptoniscan larvae Southeastern Arabian Sea notophryxus

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Amyloid-beta pathology-induced nanoscale synaptic disruption:the case of the GABA_B-GIRK assembly

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neural Regeneration Research 2025年第5期20卷 1409-1410页

作者： Rafael Lujan Alejandro Martín-Belmonte Sergi Ferré Francisco Ciruela Synaptic Structure laboratory Instituto de Biomedicina de la UCLM(IB-UCLM)Departamento de Ciencias MédicasInstituto de Investigación en Salud de Castilla-La Mancha(IDISCAM)Facultad de MedicinaUniversidad Castilla-La ManchaCampus BiosanitarioC/AlmansaAlbaceteSpain Integrative neurobiology Section National Institute on Drug AbuseIntramural Research ProgramNational Institutes of HealthBaltimoreMDUSA Pharmacology Unit Department of Pathology and Experimental TherapeuticsSchool of Medicine and Health SciencesInstitute of NeurosciencesUniversity of BarcelonaNeuropharmacology&Pain GroupNeuroscience ProgramBellvitge Institute for Biomedical ResearchL’Hospitalet de LlobregatSpain

Alzheimer's disease (AD) is characterized by an imbalance between excitatory and inhibitory brain networks,leading to aberrant homeostatic synaptic *** has progressively been recognized as syna ptopathy and syna ptic dysfunction has been identified as a key component of its pathogenesis (Schirinzi et al.,2020).Syna ptic dysfunction is believed to precede synapse loss,a primary biological correlate of cognitive decline in AD,inevita bly associated with neuronal death.

关键词： Alzheimer pathology

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Exact solutions for magnetohydrodynamic nanofluids flow and heat transfer over a permeable axisymmetric radially stretching/shrinking sheet

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Chinese Physics B 2024年第2期33卷 108-114页

作者： U.S.Mahabaleshwar G.P.Vanitha L.M.Pérez Emad H.Aly I.Pop department of Studies in Mathematics Davangere UniversityShivagangothriDavangereIndia departamento de F´ısica FACIUniversidad de Tarapac´aCasilla 7DAricaChile department of Mathematics Faculty of EducationAin Shams UniversityRoxyCairoEgypt department of Mathematics Babes-Bolyai University400084 Cluj-NapocaRomania

We report on the magnetohydrodynamic impact on the axisymmetric flow of Al_(2)O_(3)/Cu nanoparticles suspended in H_(2)O past a stretched/shrinked *** the use of partial differential equations and the corresponding thermophysical characteristics of nanoparticles,the physical flow process is *** resultant nonlinear system of partial differential equations is converted into a system of ordinary differential equations using the suitable similarity *** transformed differential equations are solved *** of the magnetic parameter,solid volume fraction and stretching/shrinking parameter on momentum and temperature distribution have been analyzed and interpreted *** skin friction and nusselt number were also *** addition,existence of dual solution was deduced for the shrinking sheet and unique solution for the stretching ***,Al_(2)O_(3)/H_(2)O nanofluid flow has better thermal conductivity on comparing with Cu/H_(2)O ***,it was found that the first solutions of the stream are stable and physically realizable,whereas those of the second ones are unstable.

关键词： magnetohydrodynamic nanofluid stretching/shrinking sheet axisymmetric flow analytical solution suction/injection

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A Stable FE-FD Method for Anisotropic Parabolic Pdes with Moving Interfaces

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Communications on Applied Mathematics and Computation 2024年第2期6卷 992-1012页

作者： Baiying Dong Zhilin Li Juan Ruiz-Alvarez School of Civil and Hydraulic Engineering NingXia UniversityYinchuan 750021NingxiaChina School of Mathematics and Computer Science NingXia Normal UniversityGuyuan 756000NingxiaChina department of Mathematics North Carolina State UniversityRaleighNC 27695-8205USA departamento de Matematica Aplicada y Estadistica Universidad Politecnica de CartagenaCartagenaSpain

In this paper,a new finite element and finite difference(FE-FD)method has been developed for anisotropic parabolic interface problems with a known moving interface using Cartesian *** the spatial discretization,the standard P,FE discretization is applied so that the part of the coefficient matrix is symmetric positive definite,while near the interface,the maximum principle preserving immersed interface discretization is *** the time discretization,a modified Crank-nicolson discretization is employed so that the hybrid FE-FD is stable and second order *** terms are needed when the interface crosses grid *** moving interface is represented by the zero level set of a Lipschitz continuous *** experiments presented in this paper confirm second orderconvergence.

关键词： Anisotropic parabolic interface problem Hybrid finite element and finite difference(FE-FD)discretization Modified Crank nicolson scheme