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World Journal of Stem Cells 2019年第9期11卷 604-617页

作者： Oleh Andrukhov Christian Behm Alice Blufstein Xiaohui Rausch-Fan Division of Conservative dentistry and Periodontology University Clinic of Dentistry Medical University of Vienna

Mesenchymal stem cells(MSCs)are considered as an attractive tool for tissue regeneration and possess a strong immunomodulatory *** tissuederived MSCs can be isolated from different sources,such as the dental pulp,periodontal ligament,deciduous teeth,apical papilla,dental follicles and *** to numerous in vitro studies,the effect of dental MSCs on immune cells might depend on several factors,such as the experimental setting,MSC tissue source and type of immune cell *** studies have shown that the immunomodulatory activity of dental MSCs is strongly upregulated by activated immune *** exert mostly immunosuppressive effects,leading to the dampening of immune cell ***,the reciprocal interaction between dental MSCs and immune cells represents an elegant mechanism that potentially contributes to tissue homeostasis and inflammatory disease *** the immunomodulatory potential of dental MSCs has been extensively investigated in vitro,its role in vivo remains obscure.A few studies have reported that the MSCs isolated from inflamed dental tissues have a compromised immunomodulatory ***,the expression of some immunomodulatory proteins is enhanced in periodontal disease and even shows some correlation with disease ***-based immunomodulation may play an essential role in the regeneration of different dental ***,immunomodulation-based strategies may be a very promising tool in regenerative dentistry.

关键词： Mesenchymal stem cells Dental tissue Immunomodulation Peripheral blood mononuclear cells Oral diseases Tissue regeneration

Erbium-doped yttrium aluminum garnet laser and advanced plateletrich fibrin+in periodontal diseases:Two case reports and review of the literature

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World Journal of clinical Cases 2022年第33期10卷 12337-12344页

作者： Kai-Seng Tan dentistry Ritz Digital Dental ClinicNew Taipei 238Taiwan

BACKGROUND The goal of periodontal disease treatment is to completely remove bacteria and promote wound *** erbium-doped yttrium aluminum garnet(Er:YAG)laser is commonly used to treat periodontal *** platelet-rich fibrin+(A-PRF+)secrets growth factors that accelerates soft-and hard-tissue regeneration and wound *** I present 2 cases of patients with oral diseases treated with a combination of Er:YAG laser and A-PRF+.CASE SUMMARY Case 1 was a female with pocket depth bone loss over 8 mm and infection of tooth 31 and 41,and severe advanced periodontitis with grade III *** 2 was a male with tooth 22 root end apical swelling and infection and alveolar bony *** outcomes were recorded at 6 and 36 *** case 1,the Er:YAG laser was used to perform open flap debridement(100 mJ/pulse,15 Hz)and remove calculus and granulation tissue(50 mJ/pulse,30 Hz).In case 2 the laser was used to create a semilunar full thickness flap incision(80 mJ/pulse,20 Hz)and eliminate the pathogen(100 mJ/pulse,15 Hz).In both patients,A-PRF+mixed with bone was used to fill bone defects,and A-PRF+autologous membranes were used to cover tension-free primary *** was no recurrent infection at 36 mo,and tissue regeneration and would healing *** Debridement with an Er:YAG laser followed by treatment with A-PRF+is effective for the treatment periodontal diseases with bone defects.

关键词： Erbium-doped yttrium aluminum garnet laser Advanced platelet-rich fibrin+ Periodontology Tissue regeneration and healing Wound healing Case report

Human dental enamel： A masterpiece investigated natural nanotechnology by TEM and t-EBSD

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Nano Research 2018年第7期11卷 3911-3921页

作者： Anjela Koblischka-Veneva Michael R. Koblischka Jorg Schmauch Matthias Hannig Experimental Physics Saafland University P. O. Box 151150 66041 Saarbrucken Germany clinic of Operative dentistry Periodontology and Preventive Dentistry Saarland University Building 73 66421 Homburg/Saar Germany

The micro- and nanostructures of human dental enamel were investigated by transmission electron microscopy （TEM） and electron backscatter diffraction （EBSD）. For this purpose, TEM slices were prepared from teeth by focused ion-beam milling. These slices enabled TEM and transmission-EBSD （t-EBSD） investigations to be performed, while standard EBSD on bulk tooth samples resulted only in Kikuchi patterns. On the TEM slices, t-EBSD enabled automated mapping. The TEM images and the EBSD data dearly elucidated the arrangement of the hydroxyapatite crystals on the nanometer scale. Information regarding the crystallographic orientation of the apatite grains enabled the deduction of novel nanotechnological building principles of the enamel structure based on a chain-like arrangement of the crystallites.

关键词： enamel microstructure transmission electron microscopy (TEM) electron backscatter diffraction (EBSD) transmission-EBSD(t-EBSD)

Efficacy of xylitol and fluoride mouthrinses on salivary mutans streptococci

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Asian Pacific Journal of Tropical Biomedicine 2011年第6期1卷 488-490页

作者： Malee Arunakul Boonyanit Thaweboon Sroisiri Thaweboon Yuwadee Asvanund Kesinee Charoenchaikorn Department of Pedriatic dentistry Faculty of DentistryMahidol University Department of Oral Microbiology Faculty of DentistryMahidol University Private clinic

Objective:To evaluate the level of salivary Mutatis streptococci(MS) after rinsing with xylitol, fluoride,and a combination of xylitol and fluoride solutions,compared with distilled water. Methods:Eighty healthy 8-9 years old subjects with high level of MS(> 10~5 CFU/mL) were equally divided into 4 *** rinsed their moutlis for 1 min with 10 mL of 0.05%(w/v) sodium fluoride(NaF),12.5%(w/v) xylitol or 0.05%(w/v) NoF + 12.5%(w/v) xylitol 3 times daily over 10 *** water rinsed group served as a ***-stimulated whole saliva samples were collected at baseline,5 weeks,and 10 weeks after rinsing to determine the level of salivary MS by culturing on Mitis Salivarius Bacitracin *** statistical significance was calculated by Kruskol Wallis,Mann Whitney U,and Wilcoxon signed-rank tests at a significant level of P< ***:Significant reductions in MS count were observed in subjects using 0.05%NaF + 12.5%xylitol over other groups within 5 weeks and after 10 weeks and 12.5%xylitol alone after 10 weeks compared with ***:The present study provides evidence for the inhibitory effect of xylitol,used in combination with fluoride,delivered in the form of mouthrinse, on salivary MS in the group of schoolchildren.

关键词： Xylitol Fluoride Mouthrinse Mutans streptococci Dental caries Salivary

Silk-based nerve guidance conduits with macroscopic holes modulate the vascularization of regenerating rat sciatic nerve

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Neural Regeneration Research 2025年第6期20卷 1789-1800页

作者： Carina Hromada Patrick Heimel Markus Kerbl LászlóGál Sylvia Nürnberger Barbara Schaedl James Ferguson Nicole Swiadek Xavier Monforte Johannes C.Heinzel Antal Nógrádi Andreas H.Teuschl-Woller David Hercher Department Life Science Engineering University of Applied Sciences Technikum WienViennaAustria Austrian Cluster for Tissue Regeneration ViennaAustria Ludwig Boltzmann Institute for Traumatology The Research Center in Cooperation with AUVAViennaAustria University clinic of dentistry Medical University of ViennaViennaAustria Department of Plastic Reconstructive and Aesthetic SurgeryLandesklinikum Wiener Neustadt2700 Wiener NeustadtAustria Department of Anatomy Histology and EmbryologyAlbert Szent-Györgyi Medical SchoolUniversity of SzegedSzegedHungary Medical University of Vienna Department of Orthopedics and Trauma SurgeryDevision of Trauma SurgeryViennaAustria Department of Hand- PlasticReconstructive and Burn SurgeryBG Unfallklinik TuebingenUniversity of TuebingenTuebingenGermany

Peripheral nerve injuries induce a severe motor and sensory deficit. Since the availability of autologous nerve transplants for nerve repair is very limited, alternative treatment strategies are sought, including the use of tubular nerve guidance conduits(tNGCs). However, the use of tNGCs results in poor functional recovery and central necrosis of the regenerating tissue, which limits their application to short nerve lesion defects(typically shorter than 3 cm). Given the importance of vascularization in nerve regeneration, we hypothesized that enabling the growth of blood vessels from the surrounding tissue into the regenerating nerve within the tNGC would help eliminate necrotic processes and lead to improved regeneration. In this study, we reported the application of macroscopic holes into the tubular walls of silk-based tNGCs and compared the various features of these improved silk^(+) tNGCs with the tubes without holes(silk^(–) tNGCs) and autologous nerve transplants in an 8-mm sciatic nerve defect in rats. Using a combination of micro-computed tomography and histological analyses, we were able to prove that the use of silk^(+) tNGCs induced the growth of blood vessels from the adjacent tissue to the intraluminal neovascular formation. A significantly higher number of blood vessels in the silk^(+) group was found compared with autologous nerve transplants and silk^(–), accompanied by improved axon regeneration at the distal coaptation point compared with the silk^(–) tNGCs at 7 weeks postoperatively. In the 15-mm(critical size) sciatic nerve defect model, we again observed a distinct ingrowth of blood vessels through the tubular walls of silk^(+) tNGCs, but without improved functional recovery at 12 weeks postoperatively. Our data proves that macroporous tNGCs increase the vascular supply of regenerating nerves and facilitate improved axonal regeneration in a short-defect model but not in a critical-size defect model. This study suggests that further optimizatio

关键词： axon regeneration blood vessel functional recovery macroporous nerve lesion peripheral nerve repair sciatic nerve silk-based nerve guidance conduit vascularization

Effect of anti-biofilm glass–ionomer cement on Streptococcus mutans biofilms

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International Journal of Oral Science 2016年第2期8卷 76-83页

作者： Su-Ping Wang Yang Ge Xue-Dong Zhou Hockin HK Xu Michael D Weir Ke-Ke Zhang Hao-Hao Wang Matthias Hannig Stefan Rupf Qian Li Lei Cheng State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University Department of Operative dentistry and Endodontics West China Hospital of Stomatology Sichuan University Biomaterials and Tissue Engineering Division Department of Endodontics Prosthodontics and Operative Dentistry University of Maryland Dental School clinic of Operative dentistry Periodontology and Preventive Dentistry Saarland University

Dental restorative materials with antimicrobial properties can inhibit bacterial colonization, which may result in a reduction of caries at tooth-filling interaction zones. This study aimed to develop antibacterial glass-ionomer cements （GIC） containing a quaternary ammonium monomer （dimethylaminododecyl methacrylate, DMADDM）, and to investigate their effect on material performance and antibacterial properties. Different mass fractions （0, 1.1% and 2.2%） of DMADDM were incorporated into the GIC. The flexure strength, surface charge density, surface roughness and fluoride release were tested. A Streptococcus mutans biofilm model was used. Exopolysaccharides （EPS） staining was used to analyze the inhibitory effect of DMADDM on the biofilm matrix. In addition, biofilm metabolic activity, lactic acid metabolism and the expression of glucosyltransferase genes g/fB, gtfC and gtfD were measured. GIC containing 1.1% and 2.2% DMADDM had flexural strengths matching those of the commercial control （P〉0.1）. DMADDM was able to increase the surface charge density but reduced surface roughness （P〈0.05）. The incorporation of 1.1% and 2.2% DMADDM elevated the release of fluoride by the GIC in the first 2 days （P〈0.05）. The novel DMADDM-modified GIC significantly reduced biofilm metabolic activity （P〈 0.05） and decreased lactic acid production （P〈 0.05）. The quantitative polymerase chain reaction （qPCR） results showed that the expression of gtfB, g/fC and gtfD decreased when mass fractions of DMADDM increased （P〈0.05）. EPS staining showed that both the bacteria and EPS in biofilm decreased in the DMADDM groups. The incorporation of DMADDM could modify the properties of GIC to influence the development of S. mutans biofilms. In this study, we investigated the interface properties of antibacterial materials for the first time. GIC containing DMADDM can improve material performance and antibacterial properties and may contribute to the better management of secondary caries.

关键词： antibacterial properties dimethylaminododecyl methacrylate glass-ionomer cement material performance Streptococcusmutans biofilms

Isolation and characterisation of human gingival margin-derived STRO-1/MACS^+ and MACS^-cell populations

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International Journal of Oral Science 2015年第2期7卷 80-88页

作者： Karim M Fawzy El-Sayed Sebastian Paris Christian Graetz Neemat Kassem Mohamed Mekhemar Hendrick Ungefroren Fred Fndrich Christof Drfer clinic for Conservative dentistry and Periodontology School of Dental MedicineChristian Albrechts University Oral Medicine and Periodontology Department Faculty of Oral and Dental MedicineCairo University Department of Operative and Preventive dentistry Charite-Universitatsmedizin Berlin Department of clinical Pathology Faculty of MedicineCairo University clinic for Applied Cellular Therapy Christian Albrechts University

Recently,gingival margin-derived stem/progenitor cells isolated via STRO-1/magnetic activated cell sorting（MACS） showed remarkable periodontal regenerative potential in *** a second-stage investigation,the present study＇s aim was to perform in vitro characterisation and comparison of the stem/progenitor cell characteristics of sorted STRO-1-positive（MACS~＋） and STRO-1-negative（MACS^-） cell populations from the human free gingival *** were isolated from the free gingiva using a minimally invasive technique and were magnetically sorted using anti-STRO-1 ***,the MACS~＋ and MACS^- cell fractions were characterized by flow cytometry for expression of CD14,CD34,CD45,CD73,CD90,CD105,CD146/MUC18 and ***-forming unit（CFU） and multilineage differentiation potential were assayed for both cell *** marker expression was examined using real-time polymerase chain reaction（PCR）.MACS~＋ and MACS- cell fractions showed plastic ***~＋ cells,in contrast to MACS- cells,showed all of the predefined mesenchymal stem/progenitor cell characteristics and a significantly higher number of CFUs（P〈0.01）.More than 95%of MACS~＋ cells expressed CD105,CD90 and CD73;lacked the haematopoietic markers CD45,CD34 and CD14,and expressed STRO-1 and CD146/***- cells showed a different surface marker expression profile,with almost no expression of CD14 or STRO-1,and more than 95%of these cells expressed CD73,CD90 and CD146/MUC18,as well as the haematopoietic markers CD34 and CD45 and ***~＋ cells could be differentiated along osteoblastic,adipocytic and chondroblastic *** contrast,MACS- cells demonstrated slight osteogenic *** MACS~＋ cells showed significantly higher expression of collagen I（P〈0.05） and collagen III（P〈0.01）,whereas MACS^- cells demonstrated higher expression of osteonectin（P〈0.05;MannWhitney）.The present study is the first to compare gingival MACS~＋ and MACS- cell populations demo

关键词： gingival progenitor populations margin periodontal cytometry collagen isolation dental sorting

In vitro effects of Salvia officinalis *** oil on Candida albicans

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Asian Pacific Journal of Tropical Biomedicine 2013年第5期3卷 376-380页

作者： Tularat Sookto Theerathavaj Srithavaj Sroisiri Thaweboon Boonyanit Thaweboon Binit Shrestha Maxillofacial Prosthetic clinic Department of ProsthodonticsFaculty of DentistryMahidol University Department of Oral Microbiology Faculty of DentistryMahidol University

Objective:To determine the anticandidal activities of Salvia officinalis L.(***)essential oil against Candida albicans(***)and the inhibitory effects on the adhesion of *** to polymethyl methacrylate(PMMA)resin ***:Disc diffusion method was first used to test the anticandidal activities of the *** *** oil against the reference strain(ATCC 90028)and 2 clinical strains of *** the minimal inhibitory concentration(MIC)and minimal lethal concentration(MLX)were determined by modified membrane *** adhesion of *** to PMMA resin surface was assessed after immersion with *** *** oil at various concentrations of 1XMIC.0.5XMIC and 0.25XMIC at room temperature for30 ***-way ANOVA was used to compare the Candida cell adhesion with the pretreatment agents and Tukey's test was used for multiple ***:*** *** oil exhibited anticandidal activity against all strains of *** with inhibition zone ranging from 40.5 mm to 19.5 *** MIC and MLC of the oil were determined as 2.780 g/L against all test *** to the effects on *** adhesion to PMMA resin surface,it was found that immersion in the essential oil at concentrations of 1XMIC(2.780 g/L).0.5XMIC(1.390 g/L)and0.25XMIC(0.695 g/L)for 30 min significantly reduced the adhesion of all 3 test strains to PMMA resin surface in a dose dependent manner(P<0.05).Conclusions:*** *** oil exhibited anticandidal activities against *** and had inhibitory effects on the adhesion of the cells to PMMA resin *** further testing and development,*** essential oil may be used as an antifungal denture cleanser to prevent candidal adhesion and thus reduce the risk of candida-associated denture stomatitis.

关键词： Salvia officinalis L. Candida albicans Essential oil PMMA resin Adhesion

Goldenhar syndrome: current perspectives

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World Journal of Pediatrics 2017年第5期13卷 405-415页

作者： Katarzyna Bogusiak Aleksandra Puch Piotr Arkuszewski Craniomaxillofacial and Oncological Surgery clinic Medical University of LodzLodzPoland Department of dentistry Medical University of LodzLodzPoland

Background:Progress in medical branches that has taken place since the first child with Goldenhare syndrome (GS) had been described in 1952 by Maurice Goldenhar,facilitated better understanding of this congenital *** also gave new perspectives and the opportunity to achieve satisfactory treatment results,mainly due to development of surgical *** sources:Based on the literature and own experience,we discussed the phenotype of presentation of GS,ethiopathogenesis,genetic counselling and treatment with particular emphasis on surgery correction of hemifacial ***:The spectrum of GS abnormalities ranges from mild to severe ones and include patients with barely noticeable facial asymmetry to very pronounced facial defect with more or less severe abnormalities of internal organs and/ or *** is characterized most commonly by impaired development of eyes,ears,lips,tongue,palate,mandible,maxifla,zygomatic and orbital structures and deformations of the teeth *** is multifactorial and dependent on genetic and environmental factors but there are still many unknowns about the syndrome which should be ***:Patients with GS due to a large variety of abnormalities and different severity of symptoms pose a challenge for *** of this necessitate an individual approach to each single patient and involvement a team of specialists in treatment *** is a complex,long-lasting,multidisciplinary process and should be divided into stages,according to patient's age,as well as the extent and severity of observed *** and pediatricians are involved in care of these patients from the onset.

关键词： abnormalities Goldenhar syndrome oculoauriculovertebral syndrome

Hepatitis C virus-associated pruritus: Etiopathogenesis and therapeutic strategies

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World Journal of Gastroenterology 2017年第5期23卷 743-750页

作者： Youssef Alhmada Denis Selimovic Fadi Murad Sarah-Lilly Hassan Youssef Haikel Mossaad Megahed Matthias Hannig Mohamed Hassan Department of Pathology University of Mississippi Medical Center Graduate School of Dental Medicine Depart-ment of Restorative DentistryDivision of Oral Health ScienceHokkaido University clinic of Operative dentistry Periodontology and Preventive DentistrySaarland University Department of Surgery Tulane University School of Medicine clinic of Dermatology University Hospital of Aachen Institut National de la Santéet de la RecherchéMédicale Department of Operative dentistry and Endodontics Dental FacultyUniversity of Strasbourg

In addition to its contributing role in the development of chronic liver diseases, chronic hepatitis C virus (HCV) infection is associated with extrahepatic manifestations, particularly, cutaneous-based disorders including those with pruritus as a symptom. Pruritus is frequently associated with the development of chronic liver diseases such as cholestasis and chronic viral infection, and the accumulation of bile acids in patients’ sera and tissues as a consequence of liver damage is considered the main cause of pruritus. In addition to their role in dietary lipid absorption, bile acids can trigger the activation of specific receptors, such as the G protein-coupled bile acid receptor (GPBA/ TGR5). These types of receptors are known to play a crucial role in the modulation of the systemic actions of bile acids. TGR5 expression in primary sensory neurons triggers the activation of the transient receptor potential vanilloid 1 (TRPV1) leading to the induction of pruritus by an unknown mechanism. Although the pathologic phenomenon of pruritus is common, there is no uniformly effective therapy available. Understanding the mechanisms regulating the occurrence of pruritus together with the conduction of large-scale clinical and evidence-based studies, may help to create a standard treatment protocol. This review focuses on the etiopathogenesis and treatment strategies of pruritus associated with chronic HCV infection.

关键词： Hepatitis C virus Pruritus Cholestasis Autotoxin Lysophosphatidic acid PI3 kinase