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Feasibility of confocal endomicroscopy in the diagnosis of pediatric gastrointestinal disorders

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World Journal of Gastroenterology 2009年第18期15卷 2214-2219页

作者： Krishnappa Venkatesh Marta Cohen Clair Evans Peter Delaney Steven Thomas Christopher Taylor Ashraf Abou-Taleb Ralf Kiesslich Mike Thomson centre for Pediatric Gastroenterology Sheffield Children's NHS Foundation TrustSheffieldS10 2THUnited Kingdom Department of Histopathology Sheffield Children's NHS Foundation TrustSheffieldS10 2THUnited Kingdom Optiscan PO Box 1066Mt WaverleyMDCVictoriaAustralia 3149Australia I.Med.Klink und Poliklinik Johannes Gutenberg University of MainzMainz 55131Germany

AIM： To evaluate the feasibility and utility of confocal laser endomicroscopy （CLE） in the description of normal gastrointestinal （GI） mucosa and in the diagnosis of GI disorders in children, in comparison to ***： Forty-four patients （19 female） median age 10.9 years （range 0.7-16.6 years） with suspected or known GI pathology underwent esophago-gastro- duodenoscopy （OGD）（n = 36） and/or ileocolonoscopy （IC）（n = 31） with CLE using sodium fluorescein and acriflavine as contrast agents. Histological sections were compared with same site confocal images by two experienced pediatric and GI histopathologists and endoscopists, ***： Duodenum and ileum were intubated in all but one patient undergoing OGD and IC. The median procedure time was 16.4 min （range 7-25 rain） for OGD and 27.9 min （range 15-45 min） for IC. A total of 4798 confocal images were compared with 153 biopsies from the upper GI tract from 36 procedures, and 4661 confocal images were compared with 188 biopsies from the ileocolon from 31 *** images were comparable to conventional histology both in normal and in pathological conditions such as esophagitis, Helicobacter pylori gastritis, celiac disease, inflammatory bowel disease, colonic heterotopia, and graft versus host ***： CLE offers the prospect of targeting biopsies to abnormal mucosa, thereby increasing diagnostic yield, reducing the number of biopsies, decreasing the burden on the histopathological services, and reducing costs.

关键词： Confocal laser endomicroscopy Histology Pediatric Gastrointestinal mucosa Gastrointestinaldisorders

Development of Bead-Based Suspension Array Technology for the Diagnosis of Hereditary Hearing Loss

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Development of Bead-Based Suspension Array Technology for th...

Metformin for Antipsychotic-Related Metabolic Syndrome in children： Fact or Fiction？

中华医学会第十五次全国医学遗传学学术会议暨中国医师协会医学遗传医师分会第一届全国学术会议暨2016年浙江省医学遗传学年会

作者： Liu Chang Zhang Liang Zhang Yan Ding Hongke Zhou Weiping Feng Defeng Zeng Yukun Liu Ling Yin Aihua Medical Genetic centre Guangdong Women and Children Hospital Maternal and children Metabolic-Genetic Key Laboratory Guangdong Women and Children Hospital

Objective Hearing loss is one of the most common birth defects and one of the most prevalent se nsorineural disorders. Given that hereditary hearing impairment exhibits incredible genetic hetero geneity, effective diagnostic methods are highly demanded by the clinical management. Method We developed a new diagnostic assay using the bead-based suspension array technolo gy(BBSAT), which can detect 20 point mutations in four genes of Asian prevalent hearing impair ment in parallel within 5 hours. More than 200 previously genotyped clinical samples were used t o determine the cut-off values and to validate the accuracy of new assay. Result In the BBSAT assay, suspended beads increase surface area to 3-D and more probes co ncentrated on beads. Besides, probes and targets mixed in the same mobile phase also generate better hybridization dynamics. With the multiplex PCR primer and probe design strategy, 20 point mutations in four genes of Asian prevalent hearing impairment can be simultaneously detected in a single assay. The results of BBSAT were completely in line with known genotypes, indicating th at the new diagnostic assay possesses excellent clinical sensitivity(100%) and specificity(100%) to identify genetic mutations of hearing loss. Conclusion The BBSAT is a rapid, accurate and cost-effective technology, suitable for molecular diagnosis of the hereditary hearing loss in large populations.

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Chinese Medical Journal 2018年第12期131卷 1490-1491页

作者： Ahmed Naguy Dalai EI-Sori AI-Manara CAP centre Kuwait Centre for Mental Health Shuwaikh State of Kuwait Paediatrics Department Cleveland Clinic Children's Cleveland Ohio USA

Use of atypical antipsychotics （AAPs） in child/adolescent psychiatry （CAP） population is rife to address a wide array of indications. Apart from the Food and Drug Administration approval in schizophrenia, bipolar mood disorders, irritability in autism spectrum disorder, and Tourette＇s syndrome, AAPs are commonly prescribed off-label for aggression in intellectual disability or attention-deficit/hyperactivity disorder, augmentation in depression and obsessive-compulsive disorder.

关键词： PLACEBO-CONTROLLED TRIAL WEIGHT-GAIN PEDIATRIC-PATIENTS DOUBLE-BLIND ADOLESCENTS MEDICATION INITIATION

The Association between Physical Education and Mental Health Indicators in Adolescents:A Cross-Sectional Study

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International Journal of Mental Health Promotion 2022年第5期24卷 783-793页

作者： Xiaoqing Hu Yan Tang School of Physical Education and Sports Training Shanghai University of SportShanghai200438China Shanghai Research centre for Physical Fitness and Health of children and Adolescents Shanghai University of SportShanghai200438China

Objective:To explore the associations between physical education attendance and mental health ***:Using data from the Global Student Health Survey,the frequency of physical education attendance,suicidality-related indicators,loneliness,bullying,and anxiety were all assessed using a standardized self-reported *** logistic regression was used to estimate the association between physical education attendance and mental health-related ***:The study included 276,169 participants from 71 coun-tries(47.3%males,aged 11–18 years old).After controlling for sex,age,food insecurity,close friends,physical activity,sedentary time,others’help,and parents’understanding,physical education attendance was not signiﬁ-cantly associated with suicidal attempts,suicidal ideation,and ***,compared with no physical edu-cation attendance,individuals attending physical education for 1,2,as well as 5 or more days had signiﬁcantly reduced odds/ratios of suicide;only 2 days of physical education attendance was associated with a lower odds ratio for suffering from *** 1 day of physical education was associated with not being bullied by ***:This study suggests that physical education attendance may not have an effective role in reducing mental health illnesses in children and *** studies are encouraged to corroborate or negate our research discoveries by using better and further improved study designs.

关键词： Physical education mental illness loneliness suicidality bullying behaviors

Vein of Galen aneurismal malformations-clinical characteristics,treatment and presentation: Three cases report

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World Journal of Clinical Cases 2019年第7期7卷 855-862页

作者： Peter Spazzapan Zoran Milosevic Tomaz Velnar Department of Neurosurgery University Medical Centre Ljubljana Department of Radiology University Medical Centre Ljubljana

BACKGROUND The vein of Galen aneurismal malformations(VGAM) are rare arteriovenous malformations of the embryonic choroid plexus. They represent about 30% of paediatric neurovascular disorders and show diverse characteristics. The VGAM is constituted by a midline dilated venous structure that receives blood from abnormal macroscopic or microscopic arteriovenous shunting vessels. Two types of VGAM exist, the choroidal and the mural. The treatment represents a challenge with the therapeutic objective to preserve the normal brain development without creating new neurological *** SUMMARY We present three cases of VGAM in the early postnatal period and their treatment. All patents were treated with the endovascular technique, which was successful. According to our experience, the endovascular technique is a safe and efficient mode of VGAM *** The objective of treatment aims to child's normal neurological development. A proper selection of patients and a thorough diagnostic workup is of vital importance. When the endovascular treatment is performed, the primary aim is not a complete VGAM exclusion at one time, which could produce a sudden reversal of blood flow with consequent venous infarction and ischemia. The aim is therefore to occlude as much of the VGAM as needed to relieve the congestive cardiac failure, to gain time and to create the conditions for a normal maturation of the neurovascular system. With the use of endovascular techniques, which represent not only the first choice of treatment but also the only safe therapeutic modality, the natural history of VGAM and their risks may be avoided safely.

关键词： Vein of Galen Aneurismal malformation children Treatment Case reports

A METHOD FOR ESTABLISHING AN ANIMAL MODEL OF LIKE-AUDITARY NEUROPATHY

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Journal of Otology 2014年第1期9卷 48-51页

作者： GUO Weiwei CHEN Peipei WEI Xiaoquan YANG Shiming Department of Otolaryngology-Head and Neck Surgery Institute of Oto-Neurobiology Centre Otolaryngology Chinese PLA General Hospital Department of Otolaryngology-Head and Neck Surgery Nanjing children's Hospital Nanjing Medical University

Objective: To establish an animal model of like-auditory neuropathy in neonatal rat. Methods The ani-mals were injected with phenylhydrazine hydrochloride or saline at 7-day of age. ABR and DPOAE were performed to assess the auditory function. The cochlea basilar membrane stretched preparation and cochlear frozen sections were prepared for immunohistochemical staining to examine the morphological change of hair cells and spiral ganglion cells (SGNs). Results At 7-day age the ABR waveI, III, V, latencies andI-III,I-V IWIs in the experimental group were significantly prolonged compared with those in the control group. The ABR thresholds were also elevated in the experimental group. We found there is no significant differ-ence in DPOAE in phenylhydrazine hydrochloride exposure group compare to control group. The cochlear hair cells showed no signs of loss in both group, but the total number of neurofilaments positive cells in SGNs were significantly reduced in the phenylhydrazine treated animals. Conclusion Our study suggests that phenylhydrazine hydrochloride can change the auditory function and induce peripheral nerve pathology by targeted mainly the SGNs in neonatal rat.

关键词： Animal model Auditory brainstem response Distortion-product otoacoustic emission auditory neuropathy

Targeted therapy for capillary-venous malformations

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Signal Transduction and Targeted Therapy 2024年第7期9卷 3024-3039页

作者： Lola Zerbib Sophia Ladraa Antoine Fraissenon Charles Bayard Marina Firpion Quitterie Venot Sanela Protic Clément Hoguin Amandine Thomas Sylvie Fraitag Jean-Paul Duong Sophie Kaltenbach Estelle Balducci Coline Lefevre Patrick Villarese Vahid Asnafi Christine Broissand Nicolas Goudin Ivan Nemazanyy Gwennhael Autret Bertrand Tavitian Christophe Legendre Nadia Arzouk Veronique Minard-Colin Caroline Chopinet Michael Dussiot Denise MAdams Tristan Mirault Laurent Guibaud Paul Isenring Guillaume Canaud UniversitéParis Cité ParisFrance INSERM U1151 Institut Necker-Enfants MaladesParisFrance Service d’Imagerie Pédiatrique Hôpital Femme-Mère-EnfantHCLBronFrance CREATIS UMR 5220 Villeurbanne 69100France Service de Radiologie Mère-Enfant Hôpital NordSaint EtienneFrance Service d’Anatomie pathologique Hôpital Necker-Enfants MaladesAP-HPParisFrance Laboratoire d’Oncohématologie Hôpital Necker-Enfants MaladesAP-HPParisFrance Pharmacie Hôpital Necker-Enfants MaladesAP-HPParisFrance Necker Bio-Image Analysis INSERM US24/CNRS UMS 3633ParisFrance Platform for Metabolic Analyses Structure Fédérative de Recherche NeckerINSERM US24/CNRS UMS 3633ParisFrance Plateforme Imageries du Vivant Universitéde ParisPARCCINSERMParisFrance Service de Néphrologie Transplantation AdultesHôpital Necker-Enfants MaladesAP-HPParisFrance Service de Transplantation Hôpital La PitiéSalpétrièreAP-HPParisFrance Department of Pediatric and Adolescent Oncology INSERM 1015Gustave RoussyUniversitéParis-SaclayVillejuifFrance Service de Physiologie&Explorations Fonctionnelles Cardiovasculaires CHU de LilleLille 59000France INSERM U1163 Laboratory of Cellular and Molecular Mechanisms of Hematological Disorders and Therapeutic ImplicationsLaboratoire d’Excellence GR-ExParisFrance Division of Oncology Comprehensive Vascular Anomalies ProgramChildren’s Hospital of PhiladelphiaPhiladelphiaPAUSA Service de Médecine Vasculaire hôpital Européen Georges-PompidouParisFrance Nephrology Research Group L’Hôtel-Dieu de Québec Research CenterDepartment of MedicineFaculty of MedicineLaval UniversityQuebecQC G1R2J6Canada Unitéde médecine translationnelle et thérapies ciblées Hôpital Necker-Enfants MaladesAP-HPParisFrance CNRS UMR8253 Institut Necker-Enfants MaladesParisFrance

Sporadic venous malformations are genetic conditions primarily caused by somatic gain-of-function mutation of PIK3CA or TEK,an endothelial transmembrane receptor signaling through *** malformations are associated with pain,bleedings,thrombosis,pulmonary embolism,esthetic deformities and,in severe cases,life-threatening *** authorized medical treatment exists for patients with venous ***,we created a genetic mouse model of PIK3CA-related capillary venous malformations that replicates patient *** showed that these malformations only partially signal through AKT *** compared the efficacy of different drugs,including rapamycin,a mTORC1 inhibitor,miransertib,an AKT inhibitor and alpelisib,a PI3Kαinhibitor at improving the lesions seen in the mouse *** demonstrated the effectiveness of alpelisib in preventing vascular malformations’occurrence,improving the already established ones,and prolonging *** these findings,we were authorized to treat 25 patients with alpelisib,including 7 children displaying PIK3CA(n=16)or TEK(n=9)-related capillary venous malformations resistant to usual therapies including sirolimus,debulking surgical procedures or percutaneous *** assessed the volume of vascular malformations using magnetic resonance imaging(MRI)for each *** demonstrated improvement in all 25 *** malformations previously considered intractable were reduced and clinical symptoms were *** showed a decrease of 33.4%and 27.8%in the median volume of PIK3CA and TEK malformations respectively,over 6 months on *** conclusion,this study supports PI3Kαinhibition as a promising therapeutic strategy in patients with PIK3CA or TEK-related capillary venous malformations.

关键词： PIK3CA malformations venous

Clinical effect of manual reduction of humeroradial joint in the treatment of typeⅠ-Ⅲfresh Monteggia fracture in children

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Chinese Journal of Traumatology 2020年第4期23卷 233-237页

作者： Yin-Qiang Cao Jia-Zhong Deng Yuan Zhang Xiao-Wei Yuan Tao Liu Jun Li Xiang Li Pan Gou Ming Li Xing Liu Department of Orthopedics Ministry of Education Key Laboratory of Child Development and DisordersNational Clinical Research Center for Child Health and DisordersChinaInternational Science and Technology Cooperation Base of Child Development and Critical DisordersChongqing Key Laboratory of PediatricsChildren's Hospital of Chongqing Medical UniversityChongqingChina Cangxi Social Insurance Hospital Guangyuan CitySichuan ProvinceChina

Purpose:To evaluate the efficacy of closed reduction on the humeroradial joint in the treatment of Bado typeⅠ,ⅡandⅢfresh Monteggia fractures in children and investigate the effect of clinical factors,including Bado classification,age and time of treatment on the success rate of closed ***:We retrospectively studied the data of children6 years)and time interval from injury to treatment(group A,day;group B,1-3 days;group C,>3 days).Results:Altogether 88 patients were employed in this study,including 58 males(65.9%)and 30 females(34.1%)aged from 1 to 10 *** were 29 cases(33.0%)of Bado typeⅠMonteggia fractures,16(18.2%)typeⅡand 43(48.7%)typeⅢ.Successful manual reduction was achieved in 79 children(89.8%)at the last *** failed 9 patients received open *** criteria showed 100%goodexcellent rate for all the *** success rate of manual reduction was 89.7%,87.5%and 90.7%in Bado typeⅠ,ⅡandⅢcases,respectively,revealing no significant differences among different Bado types(x2=0.131,p=0.937).Successful closed reduction was achieved in 13 toddlers(13/13,100%),38 preschool children(28/42,90.5%)and 28 school-age children(28/33,84.8%),suggesting no sig

关键词： children Monteggia fracture Manual reduction

Movement behaviors and their association with depressive symptoms in Brazilian adolescents:A cross-sectional study

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Journal of Sport and Health Science 2022年第2期11卷 252-259页

作者： Bruno Goncalves Galdino da Costa Jean-Philippe Chaput Marcus Vinicius Veber Lopes Luis Eduardo Argenta Malheiros Kelly Samara Silva Research centre in Physical Activity and Health Federal University of Santa CatarinaFlorianopolis88040-900Brazil Healthy Active Living and Obesity Research Group Children’s Hospital of Eastern Ontario Research InstituteOttawaON K1H 8L1Canada

Background:Physical activity,sleep,and sedentary behaviors compose 24-h movement behaviors and have been independently associated with depressive ***,it is not clear whether it is the movement behavior itself or other contextual factors that are related to depressive *** objective of the present study was to examine the associations between self-reported and accelerometer-measured movement behaviors and depressive symptoms in ***:Cross-sectional data from 610 adolescents(14-18 years old)were *** answered questions from the Center for Epidemiological Studies Depression scale and reported time spent watching videos,playing videogames,using social media,time spent in various physical activities,and daytime ***-worn accelerometers were used to measure sleep duration,sleep efficiency,sedentary time,and physical ***-effects logistic regressions were ***:Almost half of the adolescents(48%)were classified as being at high risk for depression(score≥20).No significant associations were found between depressive symptoms and accelerometer-measured movement behaviors,self-reported non-sport physical activity,watching videos,and playing ***,higher levels of self-reported total physical activity(odd ratio(OR)=0.92,95%confidence interval(95%CI):0.86-0.98)and volume of sports(OR=0.88,95%CI:0.79-0.97),in minutes,were associated with a lower risk of depression,while using social media for either 2.0-3.9 h/day(OR=1.77,95%CI:1.58-2.70)or>3.9 h/day(OR=1.67,95%CI:1.10-2.54),as well as higher levels of daytime sleepiness(OR=1.17,95%CI:1.12-1.22),were associated with a higher risk of ***:What adolescents do when they are active or sedentary may be more important than the time spent in the movement behaviors because it relates to depressive *** daytime sleepiness,promoting sports,and limiting social media use may benefit adolescents.

关键词： Accelerometry Depression Physical activity Sedentary behavior Sleep