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Quantitative trait loci analysis for root traits in synthetic hexaploid wheat under drought stress conditions

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Journal of Integrative Agriculture 2020年第8期19卷 1947-1960页

作者： lIU Rui-xuan WU Fang-kun YI Xin lIN Yu WANG Zhi-qiang lIU Shi-hang DENG Mei MA Jian WEI Yu-ming ZHENG You-liang lIU Ya-xi Triticeae Research Institute Sichuan Agricultural UniversityChengdu 611130P.R.China College of Environmental Sciences Sichuan Agricultural UniversityChengdu 611130P.R.China

Synthetic hexaploid wheat(SHW),possesses numerous genes for drought that can help breeding for drought-tolerant wheat *** evaluated 10 root traits at seedling stage in 111 F9 recombinant inbred lines derived from a F2 population of a SHW line(SHW-l1)and a common wheat line,under normal(NC)and polyethylene glycol-simulated drought stress conditions(DC).We mapped quantitative trait loci(QTls)for root traits using an enriched high-density genetic map containing 120370 single nucleotide polymorphisms(SNps),733 diversity arrays technology markers(dart)and 119 simple sequence repeats(SSRs).With four replicates per treatment,we identified 19 QTls for root traits under NC and DC,and 12 of them could be consistently detected with three or four *** novel QTls for root fresh weight and root diameter under NC explained 9 and 15.7%of the phenotypic variation respectively,and six novel QTls for root fresh weight,the ratio of root water loss,total root surface area,number of root tips,and number of root forks under DC explained 8.5–14%of the phenotypic *** seven of eight novel QTls could be consistently detected with more than three *** provide essential information for fine-mapping QTls related to drought tolerance that will facilitate breeding drought-tolerant wheat cultivars.

关键词： synthetic hexaploid wheat quantitative trait loci drought stress root traits

A High-throughput Genomic Tool： Diversity Array Technology Complementary for Rice Genotyping

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Journal of Integrative plant Biology 2006年第9期48卷 1069-1076页

作者： Yong Xie Kenneth McNally Cheng-Yun li Hei leung You-Yong Zhu National Center for Agro-biodiversity Yunnan Agricultural University Kunming 650201 China Genetic Resources Center International Rice Research Institute DAPO P.O. Box 7777 Metro Manila Philippines Entomology and plant pathology Division International Rice Research Institute DAPO P.O. Box 7777 Metro Manila Philippines

Diversity array technology （dart^TM） was a genotyping tool characterized gel-independent and high throughput. The main purpose of present study is to validate dart for rice （Oryza sativa l.）genotyping in a high throughput manner. Technically, the main objective was to generate a rice general purpose gene pool, and optimize this genomic tool in order to evaluate rice germplasm genetic diversity. To achieve this, firstly, a generalpurpose dart array was developed. Ten representatives from 24 varieties were hybridized with the general-purpose array to determine the informativeness of the clones printed on the array. The informative 1 152 clones were re-arrayed on a slide and used to fingerprint 17 of 24 germplasms. Hybridizing targets prepared from the germplasm to be assayed to the DNA array gave DNA fingerprints of germplasms. Raw data were normalized and transformed into binary data, which were then analyzed by using NTSYSpc （Numerical taxonomy system for cluster and ordination analysis, v. 2.02j） software package. The graphically displayed dendrogram derived from the array experimental data was matched with simple sequence repeats genotyping outline and varieties＇ pedigree deviation of the different varieties. Considering dart is a sequence-independent genotyping approach, it will be applied in studies of the genetic diversity and the gene mapping of diverse of organisms, especially for those crops with less-developed molecular markers.

关键词： amplified fragment length polymorphisms diversity array technology genome complexity reduction microarray rice genotyping

基于dart-Seq SNp的高密度遗传图谱的构建及野生型和栽培型西瓜杂交遗传群体偏分离基因组区域的鉴定

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中国瓜菜 2019年第8期32卷 172-173页

作者：任润生羊杏平(编译) Rumiana Ray 李苹芳徐锦华张曼刘广姚协丰 Andrzej Kilian 江苏省农业科学院蔬菜作物研究所·华东地区国家农业科技创新中心园艺作物遗传改良实验室南京210014 华东地区国家农业科技创新中心园艺作物遗传改良实验室南京210014 Division of plant and Crop Sciences School of BiosciencesUniversity of Not tinghamSutton Bonington Campus Loughborough LEI2 5RD UK dart p/l pO Box 7141 Yarralumla Canberra Australia

目的与意义:西瓜[Citrullus lanatus (Thunb.) *** Nakai]是重要的蔬菜作物,在全世界范围内都广泛种植.构建高密度的西瓜遗传图谱和不断利用新开发的标记构建饱和遗传图谱仍然是目前西瓜基因组学研究的重要内容之一.本项研究主要以西瓜... 详细信息

目的与意义:西瓜[Citrullus lanatus (Thunb.) *** Nakai]是重要的蔬菜作物,在全世界范围内都广泛种植.构建高密度的西瓜遗传图谱和不断利用新开发的标记构建饱和遗传图谱仍然是目前西瓜基因组学研究的重要内容之一.本项研究主要以西瓜自交系‘K3’和野生西瓜种‘pI189225’为亲本构建F2分离群体,利用以dartseq为基础的SNp标记构建西瓜高密度遗传连锁图谱.

关键词： SNp标记遗传图谱基因组学西瓜种高密度野生型遗传群体偏分离

localisation of loci Involved in Resistance to Diaporthe toxicaand pleiochaeta setosain White lupin (lupinus albusl.)

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Open Journal of Genetics 2014年第3期4卷 210-226页

作者： Rosy Raman Cina Vipin David J. luckett Raymond B. Cowley Gavin J. Ash John D. I. Harper Andrzej Kilian Harsh Raman dart pty ltd. (ACT) University of Canberra Canberra Australia Graham Centre for Agricultural Innovation (An Alliance between Charles Sturt University and NSW Department of primary Industries) Pugsley Place Wagga Wagga Agricultural Institute Wagga Wagga Australia NSW Department of Primary Industries Agricultural Institute Wagga Wagga Australia Graham Centre for Agricultural Innovation (An Alliance between Charles Sturt University and NSW Department of primary Industries) Pugsley Place Wagga Wagga Agricultural Institute Wagga Wagga Australia School of Agricultural & Wine Sciences Charles Sturt University Wagga Wagga Australia Graham Centre for Agricultural Innovation (An Alliance between Charles Sturt University and NSW Department of primary Industries) Pugsley Place Wagga Wagga Agricultural Institute Wagga Wagga Australia School of Agricultural & Wine Sciences Charles Sturt University Wagga Wagga Australia Graham Centre for Agricultural Innovation (An Alliance between Charles Sturt University and NSW Department of primary Industries) Pugsley Place Wagga Wagga Agricultural Institute WaggaWagga Australia NSW Department of Primary Industries Agricultural Institute Wagga Wagga Australia NSW Department of primary Industries Agricultural Institute Wagga Wagga Australia

l. albus is an annual grain-legume crop mainly grown for high-protein fodder worldwide but also to produce large seeds for human consumption as a snack-food. In order to make genetic gains in grain yield, assessment of the genetic variation in the germplasm and identification of loci associated with agronomic traits are essential. phomopsis blight (pB) and pleiochaeta root rot (pRR), caused by the fungal pathogens Diaporthe toxica and, pleiochaeta setosa respectively, are two major yield-limiting diseases of the l. albus crop. The extent of genetic diversity in 94 accessions of l. albus comprising: Australian and exotic cultivars, advanced breeding lines, and landraces originating from 26 different countries was determined utilizing pCR-based genic, and microarray-based Diversity Arrays Technology (dart™), markers. All accessions were evaluated for resistance to pB in two plant tissues (leaves and stems) using either sprayed or injected spore inoculum. A subset of 58 accessions was further evaluated for resistance to pRR by growing seedlings in spore-infested potting mix. The combined data of 724 (50 genic- and 674 dart) markers were used for cluster analysis. A subset of 324 markers with call rate ≥95% and predicted disease scores of different genotypes were used to identify marker loci accounting for phenotypic variation in pB and pRR resistance using linear regression analysis. Several markers showed significant association with pB or pRR resistance at p lts showed that favourable alleles for pB and pRR resistance are present in the diverse accessions investigated and they will provide valuable materials for lupin breeding.

关键词： l. albus Genetic Diversity phomopsis Blight pleiochaeta Root Rot dart linear Regression Analysis Genome-Wide Association Analysis

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六棱大麦抗赤霉病QTl的定位(英文)

麦类作物学报 2015年第3期35卷 324-333页

作者：张立异陈天青 MARCHAND S BElZIlE F 张爱民贵州省旱粮研究所贵州贵阳550006 Department of phytology Laval UniversityQuebec(QC)G1K 7P4Canada 植物细胞与染色体工程国家重点实验室/中国科学院遗传与发育生物学研究所北京100014

赤霉病是最严重的大麦病害之一。由于赤霉病抗性是受多基因控制的数量性状(QTl),并且一些表型性状也影响大麦赤霉病的抗病,如棱数、株高和抽穗期等,所以抗赤霉病大麦品种的选育十分困难。为了明确加拿大六棱大麦中赤霉病抗性以及相关性... 详细信息

赤霉病是最严重的大麦病害之一。由于赤霉病抗性是受多基因控制的数量性状(QTl),并且一些表型性状也影响大麦赤霉病的抗病,如棱数、株高和抽穗期等,所以抗赤霉病大麦品种的选育十分困难。为了明确加拿大六棱大麦中赤霉病抗性以及相关性状的QTls,本研究在4年中对93个家系的DH作图群体中赤霉病抗性、呕吐毒素(DON)含量、株高、抽穗期和成熟期等相关性状进行调查,并利用分子标记(444个dart和26个SSR标记)构建的连锁图谱对QTl开展复合区间作图。结果表明,本研究共检测到4个影响赤霉病的QTls,其中,2个主要的QTls定位在3H和7H染色体上,它们的加性效应为-3.44和-3.69,分别解释14.1%和17.5%的表型差异,总共解释31.6%的赤霉病抗性差异;另外2个QTls定位于7H染色体上,但二者同时也与DON含量显著相关。此外,在3H、5H和7H染色体上确定了5个影响株高的QTls,在2H、4H、5H和7H上确定了4个影响抽穗期的QTls。同时发现2个赤霉病抗性QTls和1个DON累积QTl与控制株高的QTls聚集重叠,1个赤霉病抗性QTl和抽穗期QTls重叠。这些与赤霉病抗性、株高及抽穗期等农艺性状紧密连锁的分子标记可进一步用于有效提高抗赤霉病大麦品种的选育效率。

关键词：大麦赤霉病呕吐霉素 dart标记 QTl 复合区间作图