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A viral movement protein targets host catalases for 26S proteasome-mediated degradation to facilitate viral infection and aphid transmission in wheat

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Molecular Plant 2024年第4期17卷 614-630页

作者： Shuyuan Tian Qingting Song Wenmei Zhou Jingke Wang Yanbin Wang Wei An Yunfeng Wu Lei Zhao State Key Laboratory for Crop Stress Resistance and High-Efficiency Production College of Plant ProtectionNorthwest A&F UniversityYanglingShaanxi 712100China Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau Ministry of Agriculture and Rural AffairsCollege of Plant ProtectionNorthwest A&F UniversityYanglingShaanxi 712100China Key Laboratory of Plant Protection Resources and Pest Management Ministry of EducationCollege of Plant ProtectionNorthwest A&F UniversityYanglingShaanxi 712100China

The infection of host plants by many different viruses causes reactive oxygen species(Ros)accumulation and yellowing symptoms,but the mechanisms through which plant viruses counteract RoS-mediated immunity to facilitate infection and symptom development have not been fully *** plant viruses are transmitted by insect vectors in the field,but the molecular mechanisms underlying virus-host-insect interactions are *** this study,we investigated the interactions among wheat,barley yellow dwarf virus(BYdV),and its aphid vector and found that the BYdV movement protein(MP)interacts with both wheat catalases(CATs)and the 26S proteasomeubiquitin receptor non-ATPase regulatorysubunit2homolog(PSMd2)to facilitate the 26S proteasome-mediateddegradation of CATs,promotingviral infection,disease symptom development,and aphid *** of the BYdV MP gene in wheat enhanced the degradation of CATs,which leading to increased accumulation of ROS and thereby enhanced viral ***,transgenic wheat lines overexpressing BYdV MP showed significantly reduced proliferation of wingless aphids and an increased number of winged *** with this observation,silencing of CAT genes also enhanced viral accumulation and reduced the proliferation of wingless aphids but increased the occurrence of winged *** contrast,transgenic wheat plants overexpressing TaCAT1 exhibited the opposite changes and showed increases in grain size and weight upon infection with *** assays demonstrated that BYdV MP interacts with PSMd2 and promotes 26S proteasome-mediated degradation of TaCAT1 likely in a ubiquitination-independent ***,our study reveals a molecular mechanism by which a plant virus manipulates the Ros production system of host plants to facilitate viral infection and transmission,shedding new light on the sophisticated interactions among viruses,host plants,and insect vectors.

关键词： babarley yellow dwarf virus movement protein reactive oxygen species aphid catalase 26S proteasomeubiquitin receptor PSMd2

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一湾浅浅的海水 POLONIO旗舰店

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室内设计与装修 2024年第11期 36-39页

作者： [西班牙]raúl Sánchez Luis Asin(摄影) coco(编译) raúl Sánchez

Polonio是一个与“夏天”和“海滩”这些关键词紧密相连的新的服装品牌,几年前诞生于卡达克斯--加泰罗尼亚布拉瓦海岸的一个田园诗般的村庄,靠近萨尔瓦多达利的永久居住地。在卡达克斯发展了几年后,该品牌决定在伊比沙岛的中心步行区和... 详细信息

Polonio是一个与“夏天”和“海滩”这些关键词紧密相连的新的服装品牌,几年前诞生于卡达克斯--加泰罗尼亚布拉瓦海岸的一个田园诗般的村庄,靠近萨尔瓦多达利的永久居住地。在卡达克斯发展了几年后,该品牌决定在伊比沙岛的中心步行区和商业区开设一个新的门店。新店面积仅为60m^(2),分为上下3层:底层空间设有面向街道的通高空间,通高空间之后则是半下沉式的区域,夹层可通过商店后面的螺旋楼梯进入。

关键词：螺旋楼梯底层空间加泰罗尼亚服装品牌商业区门店旗舰店下沉式

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金闪闪的生活玫瑰金公寓

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室内设计与装修 2024年第9期 46-50页

作者： [西班牙]raúl Sánchez JoséHevia(摄影) coco(编译) raúl Sánchez

玫瑰金公寓位于巴塞罗那,夹在En#sanche derecho宽敞的内部庭院和Enric Granados街之间,面积为85m^(2)。原平面很方正,标准的公寓户型,只在入口处有一个很小的L形的入户庭院。设计师希望能充分利用这个入户庭院,将它与公寓最里面的阳台... 详细信息

玫瑰金公寓位于巴塞罗那,夹在En#sanche derecho宽敞的内部庭院和Enric Granados街之间,面积为85m^(2)。原平面很方正,标准的公寓户型,只在入口处有一个很小的L形的入户庭院。设计师希望能充分利用这个入户庭院,将它与公寓最里面的阳台贯通,使空气对流。重新布局后的公寓,通道位于中心位置,连通阳台和入户庭院。从入户庭院开始,穿过两侧的卧室,到达宽敞的公共空间,包括一侧的客厅和另一侧的餐厅厨房。

关键词：空气对流巴塞罗那玫瑰金公寓公共空间庭院

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Assessment of Nitrates and Nitrites in Borehole Water from the Southern and the Northern Region of Côte d’Ivoire (West Africa)

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Open Journal of Modern Hydrology 2024年第2期14卷 87-105页

作者： Jean Stéphane Claon Kouassi Kouakou Serge Sérikipré Laurent Seka M’Bassidjé Arsène N’Guettia Kossonou Roland Traoré Aïcha djamanallico Joseph Kouadio Kouakou Luc Analytical Sciences and Public Health department University of Félix Houphou&#235t-Boigny Abidjan C&#244te d’Ivoire Water Quality Control and Analysis Laboratory (LACQUE) Office National de l’Eau Potable (ONEP) Abidjan C&#244te d’Ivoire Biology and Health Laboratory University of Félix Houphou&#235t-Boigny Abidjan C&#244te d’Ivoire department of Medical and fundamental Biochemistry Institut Pasteur of C&#244te d’Ivoire Abidjan C&#244te d’Ivoire Laboratory of Environmental Sciences (LSE) University of Nangui Abrogoua Abidjan C&#244te d’Ivoire

This study aimed to evaluate the quality of water from village boreholes by measuring physicochemical parameters such as nitrates, nitrites, and total organic carbon (TOC). forty-five (45) village pumps from the Southern (Basse Côte) and the Northern (Korhogo) region of Cte d’Ivoire (west Africa) were sampled. Physicochemical parameters such as temperature, pH, conductivity at 25˚C, and turbidity were determined in situ, while nitrite and nitrate were analyzed according to ISO 10304-1 (2007) standard and total organic carbon (TOC) by Nf EN 1484 (1997) standard. The results showed that the borehole waters of the Basse Côte and Korhogo analyzed are acidic, with an average temperature of 27.51˚C ± 0.16˚C and 29.95˚C ± 0.51˚C respectively for the Basse Côte and Korhogo regions. The borehole waters of the Basse Côtedo not contain nitrites, while those of Korhogo have average nitrite contents of 0.32 mg/l. The average nitrate rate in the waters of the Basse Côte and Korhogo are 12.08 ± 2.11 mg/l and 11.03 ± 3.18 mg/l respectively. The average TOC concentration of the waters of the Basse Côte is 1.28 ± 0.32 mg/l and that of Korhogo is 0.56 ± 0.09 mg/L. The study showed that the borehole waters of the Basse Côte and Korhogo have average temperatures between 27.4˚C and 29.95˚C with a slightly acidic pH value and acceptable salinity. The TOC concentrations obtained at the different sampling points were all below the french standard (2 mg/L) except for certains pumps of the Basse Côte. The water samples from the Basse Côte were devoid of nitrite. On the other hand, those from Korhogo revealed the presence of nitrite. Also, the borehole waters of the regions of the Basse Côte and Korhogo contain relatively high nitrate contents, presumably due to anthropometric activity. Overall, our study on the quality of drinking water showed that the waters analyzed are in compliance with international standards and safe for consumption.

关键词： drinking Water pH Turbidity TOC Nitrates Nitrites Health Effect

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Solar wind ion charge state distributions and compound cross sections for solar wind charge exchange X-ray emission

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Earth and Planetary Physics 2024年第1期8卷 105-118页

作者： dimitra Koutroumpa LATMOS-OVSQ CNRSUVSQ Paris-SaclaySorbonne Université11 Boulevard d’Alembert78280GuyancourtFrance

Solar Wind Charge eXchange X-ray(SWCX) emission in the heliosphere and Ea rth’s exosphere is a hard to avoid signal in soft Xray obse rvations of astrophysical *** the other hand,the X-ray imaging possibilities offered by the SWCX process has led to an increasing number of future dedicated space missions for investigating the solar wind-terrestrial inte ractions and magnetospheric *** both cases,accurate modelling of the SWCX emission is key to correctly interpret its signal,and remove it from obse rvations,when *** this paper,we compile solar wind abundance measurements from ACE for different solar wind types,and atomic data from literature,including charge exchange cross-sections and emission probabilities,used fo r calculating the compound cross-section a for the SWCX X-ray *** calculate a values for charge-exchange with H and He,relevant to soft X-ray energy bands(0.1-2.0 keV)for various solar wind types and solar cycle conditions.

关键词： solar wind charge exchange X-rays magnetosphere heliosphere

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Leveraging the potential of big genomic and phenotypic data for genome-wide association mapping in wheat

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The Crop Journal 2024年第3期12卷 803-813页

作者： Moritz Lell Yusheng Zhao Jochen C.Reif Leibniz Institute of Plant Genetics and Crop Plant Research(IPK) Corrensstraße 3D-06466 SeelandGermany

Genome-wide association mapping studies(GWAS)based on Big data are a potential approach to improve marker-assisted selection in plant *** number of available phenotypic and genomic data sets in which medium-sized populations of several hundred individuals have been studied is rapidly *** these data and using them in GWAS could increase both the power of QTL discovery and the accuracy of estimation of underlying genetic effects,but is hindered by data heterogeneity and lack of *** this study,we used genomic and phenotypic data sets,focusing on Central European winter wheat populations evaluated for heading *** explored strategies for integrating these data and subsequently the resulting potential for *** interoperability between data sets was greatly aided by some overlapping genotypes and a linear relationship between the different phenotyping protocols,resulting in high quality integrated phenotypic *** this context,genomic prediction proved to be a suitable tool to study relevance of interactions between genotypes and experimental series,which was low in our *** to expectations,fewer associations between markers and traits were found in the larger combined data than in the individual experimental ***,the predictive power based on the marker-trait associations of the integrated data set was higher across data ***,the results show that the integration of medium-sized to Big data is an approach to increase the power to detect QTL in *** results encourage further efforts to standardize and share data in the plant breeding community.

关键词： Big data Genome-wide association study data integration Genomic prediction Wheat

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dietary saccharin sodium supplementation improves the production performance of dairy goats without residue in milk in summer

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ANIMAL NUTRITION 2024年第3期18卷 166-176页

作者： Zhang, Xiongfei Lv, Jirong Hui, Jingtao Wu, Ao Zhao, Lichao feng, Linyu deng, Lu Yu, Miao Liu, feng Yao, Junhu Lei, Xinjian Northwest A&f Univ Coll Anim Sci & Technol Yangling 712100 Shaanxi Peoples R China Northwest A&f Univ Key Lab Livestock Biol Yangling 712100 Shaanxi Peoples R China dadHank Biotechnol Corp Sichuan Prov Anim Feeding Regulat Ctr Chengdu 611130 Sichuan Peoples R China

The purpose of this study was to investigate the effects of dietary saccharin sodium supplementation on production performance, serum biochemical indicators, and rumen fermentation of dairy goats in summer. Twelve Guanzhong dairy goats with similar body weight, days in milk, and milk yield were randomly divided into two dietary treatments: (1) CON: basal diet; (2) SS: basal diet + 150 mg/kg saccharin sodium on the basis of dry matter. The experiment lasted 35 d, including 7 d for adaptation and 28 d for dietary treatments, sampling and data collection. Each dairy goat was housed individually in a clean separate pen with ad libitum access to diet and water. The goats fed SS diet had increased dry matter intake (dMI; P = 0.037), 4% fat corrected milk yield (P = 0.049), energy corrected milk yield (P = 0.037), milk protein yield (P = 0.031), and total solids yield (P = 0.036). Serum activity of aspartate aminotransferase (P = 0.047) and concentrations of 70-kda heat shock protein (P = 0.090), malondialdehyde (P = 0.092), and total protein (P = 0.057) were lower in goats fed SS diet than those fed CON diet. Supplementation of saccharin sodium tended to increase activity of glutathione peroxidase in serum (P = 0.079). The concentrations of rumen total volatile fatty acid (P = 0.042) and butyrate (P = 0.038) were increased by saccharin sodium supplementation. dietary supplementation of saccharin sodium increased the relative abundance of Lachnobacterium (P = 0.022), Pseudoramibacter (P = 0.022), Shuttleworthia (P = 0.025), and Syntrophococcus (P = 0.037), but reduced the relative abundance of Prevotella_1 (P = 0.037) and Lachnospiraceae_UCG_008 (P = 0.037) in rumen. Saccharin sodium was observed in feces and urine of goats fed diet supplemented with saccharin sodium, but saccharin sodium was undetectable in the milk of goats receiving SS diet. In conclusion, administration of saccharin sodium was effective in increasing fat and energy corrected milk yield by increasing dM

关键词： dairy goat Heat stress Production performance Rumen fermentation Rumen microorganisms Saccharin sodium HEAT-STRESS PHYSIOLOGICAL-RESPONSES RUMINAL fERMENTATION GROWTH-PERfORMANCE RUMEN fERMENTATION fEEdING-BEHAVIOR OXIdATIVE STATUS CLIMATE-CHANGE CALVES AdAPTATION

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Effect of sodium nitroprusside on the microrheological properties of red blood cells in di®erent media

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Journal of Innovative Optical Health Sciences 2024年第5期17卷 23-31页

作者： Petr Ermolinskiy Matvey Maksimov Andrei Lugovtsov Alexey Muravyov Irina Tikhomirova Alexander Priezzhev faculty of Physics M.V.Lomonosov Moscow State University Moscow 119991Russia faculty of Physical Education K.d.Ushinsky Yaroslavl State Pedagogical University Yaroslavl 150000 Russia faculty of Natural Sciences K.d.Ushinsky Yaroslavl State Pedagogical University Yaroslavl 150000 Russia

Red blood cell(RBC)aggregation as well as their deformation signi¯cantly affects blood *** processes depend on various factors,one of which is concentration of the nitric oxide,one of the main signaling molecule in the *** purpose of this study was to investigate the effect of nitric oxide on the microrheological properties of red blood cells(RBCs)in RBC samples of various media after the addition of nitric oxide donor sodium nitroprusside in *** properties were measured using laser aggregometer and ektacytometer based on diffuse light scattering and diffraction of laser light on a suspension of RBCs,*** study found that heparin-stabilized blood showed increased RBC aggregation and deformation with sodium nitroprusside concentrations of 100,and 200M,while EdTA-stabilized blood showed slightly decreased aggregation and unchanged *** washed RBCs in dextran solution,the addition of sodium nitroprusside(in the concentrations of 100,and 200M)resulted in decreased aggregation and increased ***-ndings aid in our understanding of nitric oxide's effect on RBC microrheological properties.

关键词： Sodium nitroprusside nitric oxide red blood cells RBC aggregation diffuse light scattering laser diffractometer

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Single‑cell sequencing reveals the reproductive variations between primiparous and multiparous Hu ewes

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Journal of Animal Science and Biotechnology 2024年第2期15卷 614-631页

作者： Ting Ge Yifan Wen Bo Li Xiaoyu Huang Shaohua Jiang Enping Zhang Key Laboratory of Animal Genetics Breeding and Reproduction of Shaanxi ProvinceCollege of Animal Science and TechnologyNorthwest A&F UniversityYangling 712100China

Background In the modern sheep production systems,the reproductive performance of ewes determines the economic profitability of *** the genetic mechanisms underlying differences in the litter size is important for the selection and breeding of highly prolific *** sheep,a high-quality Chinese sheep breed,is known for its high fecundity and is often used as a model to study prolificacy *** the current study,animals were divided into two groups according to their delivery rates in three consecutive lambing seasons(namely,the high and low reproductive groups with≥3 lambs and one lamb per season,n=3,respectively).The ewes were slaughtered within 12 h of estrus,and unilateral ovarian tissues were collected and analyzed by 10×Genomics single-cell RNA *** A total of 5 types of somatic cells were identified and corresponding expression profiles were mapped in the ovaries of each ***,the differences in the ovary somatic cell expression profiles between the high and low reproductive groups were mainly clustered in the granulosa ***,four granulosa cell subtypes were *** analysis revealed that the abundance of JPH1 expression and the reduction of LOC101112291 expression could lead to different evolutionary directions of the granulosa ***,the expression levels of fTH1 and fTL in mural granulosa cells of the highly reproductive group were significantly *** genes inhibit necroptosis and ferroptosis of mural granulosa cells,which helps prevent follicular *** This study provides insights into the molecular mechanisms underlying the high fecundity of Hu *** differences in gene expression profiles,particularly in the granulosa cells,suggest that these cells play a critical role in female *** findings also highlight the importance of genes such as JPH1,LOC101112291,fTH1,and fTL in regulating granulosa cell function and follicular development.

关键词： Granulosa cells Hu sheep Lambing number Ovarian somatic cells Single-cell RNA sequencing

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Arginine promotes seed energy metabolism,increasing wheat seed germination at low temperature

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The Crop Journal 2024年第4期12卷 1185-1195页

作者： Jiayu Li Zhiyuan Li Yangyang Tang Jianke Xiao Vinay Nangia Yang Liu College of Agronomy Northwest A&F UniversityYangling 712100ShaanxiChina International Center for Agricultural Research in the dry Areas Rabat 999055Morocco

Low temperatures during germination inhibit seed growth,lead to small and weak seedlings,and significantly reduce the wheat *** the adverse effects of low temperature on wheat seed germination is highly important for achieving high and stable wheat *** this study,Tongmai 6(insensitive)and Zhengmai 113(sensitive),which have different low-temperature sensitivities during germination were treated with low temperature during *** transcriptome,metabolome and physiological data revealed that low temperature decreased the germination rate,downregulated the expression of a large number of genes involved in regulating glycometabolism,and inhibited carbon,nitrogen(especially amino acids)and energy metabolism in the *** content increased at low temperature,and its increase in the low-temperature-tolerant variety was significantly greater than that in the sensitive *** priming experiment showed that treatment with an appropriate concentration of arginine improved the seed germination *** conversion of starch to soluble sugar significantly increased under exogenous arginine conditions,the content of key metabolites in energy metabolism increased,and the utilization of ATP in the seeds *** together,arginine priming increased seed germination at low temperature by relieving inhibition of seed carbon and nitrogen metabolism and improving seed energy metabolism.

关键词： Low temperature Seed germination Arginine Energy metabolism Wheat

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