检索结果-南通市图书馆

Journal of Genetics and Genomics 2024年第8期51卷 836-843页

作者： Shibo Hou Jingheng Chen Ruobing Feng Xuejiao Xu Nan Liang Jackson Champer Center for Bioinformatics Center for Life SciencesSchool of Life SciencesPeking UniversityBeijing 100871China

CRISPR homing gene drives have considerable potential for managing populations of medically and agriculturally significant *** operate by Cas9 cleavage followed by homology-directed repair,copying the drive allele to the wild-type chromosome and thus increasing in frequency and spreading throughout a ***,resistance alleles formed by end-joining repair pose a significant *** address this,we create a homing drive targeting the essential hairy gene in Drosophila *** resistance alleles are recessive lethal,while drive carriers have a recoded“rescue”version of *** drive inheritance rate is moderate,and multigenerational cage studies show drive spread to 96%–97%of the ***,the drive does not reach 100%due to the formation of functional resistance alleles despite using four *** alleles have a large deletion but likely utilize an alternate start ***,revised designs targeting more essential regions of a gene may be necessary to avoid such functional *** of the rescue element’s native 3'UTR with a homolog from another species increases drive inheritance by 13%–24%.This was possibly because of reduced homology between the rescue element and surrounding genomic DNA,which could also be an important design consideration for rescue gene drives.

关键词： Genedrive Homing drive Rescueelement Population modification Multiplexed gRNAs Resistance alleles Cage study

The influence of wing morphology upon intraspecific divergence in birds: A global study of subspecies richness

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Avian Research 2024年第2期15卷 196-201页

作者： Haiying Fan Weibin Guo Buge Lin Zhiqing Hu Changcao Wang Shaobin Li school of life sciences Jinggangshan UniversityJi’An343009China State Key Laboratory of Biocatalysis and Enzyme Engineering School of Life SciencesHubei UniversityWuhan430062China College of life Science Yangtze UniversityJingzhou434023China

Since the time of Darwin,subspecies have been recognized as an initial stage in the evolution of ***,the impact of dispersal on subspecies richness in birds globally has received little attention,despite dispersal being a key factor in determining the rate of evolution and playing a significant role in evolutionary ***,it is important to conduct a comprehensive study to address this *** this study,we aimed to assess the association between subspecies richness(measured by the number of subspecies)and dispersal ability(measured by the hand-wing index)on a global scale,using a dataset of over 7000 bird *** results revealed a negative correlation between dispersal ability and the number of subspecies,without any quadratic *** wing is a crucial phenotypic trait for birds,and the concept of subspecies is important in speciation theory and conservation ***,our findings not only enhance our understanding of the association between avian morphological traits and evolutionary divergence but also have implications for the conservation of avian species diversity.

关键词： Dispersal Gene flow Hand-wing index Subspecies richness

维普期刊数据库

Implications of mosquito metabolism on vector competence

在线全文

学校读者我要写书评

暂无评论

Insect Science 2024年第3期31卷 674-682页

作者： Li Gao Wenxu Yang Jingwen Wang State Key Laboratory of Genetic Engineering School of Life SciencesFudan UniversityShanghaiChina Ministry of Education Key Laboratory of Contemporary Anthropology School of Life SciencesFudan UniversityShanghaiChina

Mosquito-borne diseases(MBDs)annually kill nearly half a million *** to the lack of effective vaccines and drugs on most MBDs,disease prevention relies primarily on controlling *** huge efforts having been put into mosquito control,eco-friendly and sustainable mosquito-control strategies are still lacking and urgently *** mosquito-transmitted pathogens have lost the capacity of de novo nutrition biosynthesis,and rely on their vertebrate and invertebrate hosts for sustenance during the long-term obligate parasitism ***,a better understanding of the metabolic interactions between mosquitoes and pathogens will contribute to the discovery of novel metabolic targets or regulators that lead to reduced mosquito populations or vector *** review summarizes the current knowledge about the effects of mosquito metabolism on the transmission of multiple *** also discuss that research in this area remains to be explored to develop multiple biological prevention and control strategies for MBDs.

关键词： flaviviruses mosquito metabolism Plasmodium vector competence

Exploring lysosomal biology: current approaches and methods

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Biophysics Reports 2024年第2期10卷 111-120页

作者： Qiuyuan Yin Chonglin Yang State Key Laboratory of Conservation and Utilization of Bio-resources in Yunnan and Center for life sciences School of Life Sciences Yunnan University Southwest United Graduate school

Lysosomes are the degradation centers and signaling hubs in the cell. Lysosomes undergo adaptation to maintain cell homeostasis in response to a wide variety of cues. Dysfunction of lysosomes leads to aging and severe diseases including lysosomal storage diseases(LSDs), neurodegenerative disorders,and cancer. To understand the complexity of lysosome biology, many research approaches and tools have been developed to investigate lysosomal functions and regulatory mechanisms in diverse experimental systems. This review summarizes the current approaches and tools adopted for studying lysosomes, and aims to provide a methodological overview of lysosomal research and related fields.

关键词： Lysosome Method Cultured cell C. elegans Mice

Females adopt sexual catalepsy to facilitate mating

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

current Zoology 2024年第2期70卷 174-181页

作者： Jihe Liu Keke Liu Yu Tang Weihua Wang Xiang Xu Jianhui Liang Yonghong Xiao Mark A.Elgar College of life sciences Jinggangshan UniversityJi'anJiangxi 343009China Pharmaceutical Center Tsinghua UniversityBeijing 100084China College of life sciences Hunan Normal UniversityChangshaHunan 410081China school of Pharmaceutical sciences Peking UniversityBeijing 100083China school of Biosciences University of MelbourneMelbourneVictoria 3010Australia school of life sciences and Technology Tongii UniversityShanghai 200092China

Theory predicts that males and females of dioecious species typically engage in an evolutionary sexual conflict over the frequency and choice of mating *** sexual cannibalism,a particularly dramatic illustration of this conflict,is widespread in certain animal taxa including ***,females of some funnel weaving spiders that are generally aggressive to conspecifics enter a cataleptic state after male courtship,ensuring the males can mate without risk of *** this study,we demonstrated that the physical posture and duration,metabo-lites,and central neurotransmitters of females of Aterigena aculeata in sexual catalepsy closely resemble females in thanatosis but are distinct from those in anesthesia,indicating that the courted females feign death to eliminate the risk of potentially aggressive responses and thereby allow preferred males to *** the taxonomically widespread thanatosis,which generally represents a deceptive visual signal that acts against the interest of the receivers,sexual catalepsy of females in the funnel weaving spiders may deliver a sexual-receptive signal to the courting males and thereby benefit both the signal senders and ***,sexual catalepsy in *** may not reflect a conflict butrather a confluence of interest betweenthe sexes.

关键词： female catalepsy funnel weaving spiders mate choice mating strategy sexual behavior.

Eurasian otters prefer to prey on religious released nonnative fish on the Qinghai-Tibetan Plateau

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

current Zoology 2024年第4期70卷 472-479页

作者： Qiaoyun Wang Xuesong Han Zichen Wang Kaidan Zheng Zhengyi Dong Peng Zhang Pengfei Fan Lu Zhang school of life sciences Sun Yat-Sen UniversityGuangzhou 510275China State Key Laboratory of Biocontrol School of Life SciencesSun Yat-sen UniversityGuangzhou 510275China school of life sciences Peking UniversityBeijing 100871China Shan Shui Conservation Center Beijing100871China school of Ecology Sun Yat-sen UniversityShenzhenGuangdong 518107China

Religious wildlife release is prevalent worldwide,especially in Asia *** is one of the anthropogenic pathways to cause biological *** fish release is common on the Qinghai-Tibetan Plateau,yet few studies have assessed the influences of religious fish release on local ***,a city on the Qinghai-Tibetan Plateau,we interviewedlocal people,conducted fish trap surveys in local rivers,and exam-ined the diet of Eurasian otters Lutra lutra using a fecal DNA metabarcoding *** found that fish release started at least in 1980-1990s in *** residents released fish in large amounts and released fish were usually exotic commercial fish purchased from *** such long-term and intensive fish release activities,released fish were few in local *** the other hand,Eurasian otters mainly prey on fish and released fish accounted for~20%of relative read abundance of prey DNA in otters'diet,indicating their high preference on released *** study suggested that religious fish release may provide additional food resources for otters,whereas otters,as a top predator in local rivers,may deplete non-native fish once they were released and,therefore,reduce the probability of colonization of released fish,although further studies are required to assess otters'*** study revealed otters'diet in Yushu,providing basic information for local otter management and ***,it represents a case showing that native predators prey on religious released animals,implying a probable direction forcontrolling invasive species through nativepredator conservation.

关键词： biological invasion Buddhism Eurasian otter prey composition religious wildlife release scat DNA.

Phenotype-Directed DNA Nanomachine for in Situ Analysis of Stem Cell-like Subpopulation in Breast Cancer

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

CCS Chemistry 2024年第4期6卷 1034-1046页

作者： Ya Cao Huiru Mao Lingjun Sha Qi Liu Bing Han Jing Zhao Genxi Li Center for Molecular Recognition and Biosensing Shanghai Engineering Research Center of Organ RepairSchool of Life SciencesShanghai UniversityShanghai 200444 State Key Laboratory of Analytical Chemistry for life Science School of Life SciencesNanjing UniversityNanjing 210023

The cancer stem cell hypothesis provides a basis for prediction of the recurrence and risk of metastasis in breast ***,the unique expression pattern of stemness markers and the presence of nonstem-like cancer cells with varied phenotypes have brought great challenges to the characterization of breast cancer stem *** address these challenges,a phenotype-directed DNA nanomachine has been designed for high-accuracy labeling and in situ analysis of the stem cell-like subpopulation in breast *** key for the design is to use cell surfaceanchored inputs to activate the nanomachine,which undergoes different branch migration pathways such that the signal strand can only be brought onto the cancer cells having the stem cell-like *** sensitive determination and single-step isolation of the stem cell-like subpopulation were achieved by incorporating functional groups into the signal strand such that the nanomachine was successfully applied in a tumor-bearing mouse ***,the approach provides for a substantial improvement in capability for the analysis of the breast cancer stem cell-like subpopulation,and it is expected that the new approach will advance the use of DNA nanomachines in cancer-related studies.

关键词： breast cancer DNA nanomachine stem cell-like subpopulation electrochemical determination single-step isolation phenotypic analysis

Assessment of four different dietary amino acid profiles recommended for ISA Brown layer hens: A validation study

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Animal Nutrition 2024年第2期17卷 438-446页

作者： Mehdi Toghyani Shemil Macelline Juliano C.de Paula Dorigam Peter V.Chrystal Peter H.Selle Sonia Y.Liu school of life and Environmental sciences Faculty of Science The University of Sydney Poultry Research Foundation The University of Sydney Evonik Operations GmbH

The current feeding study was designed to validate the two dietary essential amino acid profiles(EAAP)established based on linear broken-line(LBL) and quadratic broken-line(QBL) models, in a previous study, against Evonik(AMINOHen) and breeder recommendations for ISA Brown layers for peak production(PP, 20 to 44 weeks of age), and post peak production(post PP, 44 to 75 weeks of age). The EAAP based on LBL models on average had 19.5% and 26.0% lower digestible AA(Lys, Met + Cys, Thr, Trp, Ile and Val), than the EAAP based on QBL models for PP and post PP, respectively. The EAAP based on AMINOHen and breeder recommendation had lower digestible AA than QBL, and higher EAAP than LBL models for both production phases. At 20 weeks of age, 224 ISA Brown layer hens were weighed and randomly allocated to individual battery cages. Each of the four diets was replicated 8 times with 7 birds per replicate. Egg production was recorded daily, and egg weights were measured at the end of each week. Feed consumption was measured at the end of each period. The egg production rate was not significantly affected by the diets and remained at around 98.0%(PP) and 95.0%(post PP)(P > 0.05). Birds fed diets based on LBL recommendation consistently laid smaller eggs, resulting in a lower egg mass(59.8 vs. 62.0 g egg/hen per day during PP, and 60.3 vs. 63.0 g egg/hen per day during post PP; P 0.05). The highest feed conversion ratio(FCR) during PP(P = 0.067) and post PP(P < 0.05) was recorded for the birds offered diets based on LBL recommendation. In conclusion, all four EAAP tested in this study support an above average egg production rate. However, the EAAP based on LBL models may potentially decrease the input feed cost per kilogram of eggs but are not set to optimise FCR and maximise egg mass.

关键词： Ideal amino acid profile Regression model Egg production Egg mass

Arbuscular mycorrhizae status of host plant affects offspring’s phenotype and transcriptome expression in a low-phosphorus environment

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Journal of Plant Ecology 2024年第2期17卷 173-187页

作者： Shijun Liu Jing Xu Hong Huang Jianjun Tang Xin Chen Key Laboratory of Plant Biotechnology of Liaoning Province School of Life SciencesLiaoning Normal UniversityDalian 116081China College of life sciences Zhejiang UniversityHangzhou 310058China The Institute of Environmental Resources and Soil Fertilizers Zhejiang Academy of Agricultural SciencesHangzhou 310021China

Although the effects of arbuscular mycorrhizal fungi(AMF)on host plants have been well documented,whether the effects of AMF on parental generations affect offspring performance is not fully *** conducted a common garden experiment to determine whether AMF status of host plants(Medicago truncatula)affects phenotype and transcriptome expression of their *** from four type parental treatments(low-phosphorus(P)soil without AMF,low-P soil with AMF,high-P soil without AMF and high-P soil with AMF)were grown under low-P(LPS)and normal-P soil(OHS)*** fowering pattern of LP offspring was similar to their parents,such that plants with AMF fowered earlier than those without AMF under OHS condition but were opposite under LPS *** transcriptome differential analysis showed that some differential transcripts(45 for parental plants growing under low-P condition and 3 for parental plants growing under high-P condition)expression patterns between offspring were comparable,and that only affected by parental AMF status regardless of the P environment that offspring was ***(146 for parental plants growing under low-P condition and 2 for parental plants growing under high-P condition),however,were affected both by the parental AMF status and the offspring P *** addition,the number of differential transcripts between offspring whose parental plants grew under high-P condition was far less than under low-P *** results indicate that AMF may not only affect the current generation of host plants but also affect the offspring especially when their parents have experienced a stressful environment.

关键词： arbuscular mycorrhizal fungi low-phosphorus environment fowering pattern transgenerational effects transcriptome differential analysis

Picroside Ⅱ promotes HSC apoptosis and inhibits the cholestatic liver fibrosis in Mdr2^(−/−)mice by polarizing M1 macrophages and balancing immune responses

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Natural Medicines 2024年第7期22卷 582-598页

作者： JIA Kexin MA Zhi ZHANG Yinhao XIE Kaihong LI Jianan WU Jianzhi QU Jiaorong LI Fanghong LI Xiaojiaoyang school of life sciences Beijing University of Chinese MedicineBeijing 100029China school of Chinese Materia Medica Beijing University of Chinese MedicineBeijing 100029China

Liver fibrosis is characterized by chronic inflammatory responses and progressive fibrous scar *** play a central role in the pathogenesis of hepatic fibrosis by reconstructing the immune *** Ⅱ(PIC Ⅱ),extracted from Picrorhizae Rhizoma,has demonstrated therapeutic potential for various liver ***,the mechanisms by which macrophage polarization initiates immune cascades and contributes to the development of liver fibrosis,and whether this process can be influenced by PIC Ⅱ,remain *** the current study,RNA sequencing and multiple molecular approaches were utilized to explore the underlying mechanisms of PIC Ⅱ against liver fibrosis in multidrug-resistance protein 2 knockout(Mdr2^(−/−))*** findings indicate that PIC Ⅱ activates M1-polarized macrophages to recruit natural killer cells(NK cells),potentially via the CXCL16-CXCR6 ***,PIC Ⅱ promotes the apoptosis of activated hepatic stellate cells(aHSCs)and enhances the cytotoxic effects of NK cells,while also reducing the formation of neutrophil extracellular traps(NETs).Notably,the anti-hepatic fibrosis effects associated with PIC Ⅱ were largely reversed by macrophage depletion in Mdr2^(−/−)***,our research suggests that PIC Ⅱ is a potential candidate for halting the progression of liver fibrosis.

关键词： Liver fibrosis PicrosideⅡ M1 macrophage Hepatic stellate cell Natural killer cell Neutrophil

维普期刊数据库