检索结果-南通市图书馆

Interactions between myoblasts and macrophages under high glucose milieus result in inflammatory response and impaired insulin sensitivity

引用

World Journal of Diabetes 2024年第7期15卷 1589-1602页

作者： Wei Luo Yue Zhou Li-Ying Wang Lei Ai Department of sports and Health Sciences Nanjing Sport InstituteNanjing 210014Jiangsu ProvinceChina Department of Exercise Physiology Beijing Sport UniversityBeijing 100084China Department of sports Physiology Research Jiangsu Research Institute of Sports ScienceNanjing 210033Jiangsu ProvinceChina

BACKGROUND Skeletal muscle handles about 80% of insulin-stimulated glucose uptake and become the major organ occurring insulin resistance(IR).Many studies have confirmed the interactions between macrophages and skeletal muscle regulated the inflammation and regeneration of skeletal ***,despite of the decades of research,whether macrophages infiltration and polarization in skeletal muscle under high glucose(HG)milieus results in the development of IR is yet to be elucidated.C2C12 myoblasts are well-established and excellent model to study myogenic regulation and its responses to *** exploration of macrophages'role in myoblasts IR and the dynamics of their infiltration and polarization is *** To evaluate interactions between myoblasts and macrophages under HG,and its effects on inflammation and IR in skeletal *** We detected the polarization status of macrophages infiltrated to skeletal muscles of IR mice by hematoxylin and eosin and immunohistochemical ***,we developed an in vitro co-culture system to study the interactions between myoblasts and macrophages under HG *** effects of myoblasts on macrophages were explored through morphological observation,CCK-8 assay,Flow Cytometry,and enzyme-linked immunosorbent *** mediation of macrophages to myogenesis and insulin sensitivity were detected by morphological observation,CCK-8 assay,Immunofluorescence,and 2-NBDG *** The F4/80 and co-localization of F4/80 and CD86 increased,and the myofiber size decreased in IR group(P0.8).In McM+HG group,F4/80+CD86+CD206-cells,monocyte chemoattractant protein 1,TNFα,IL-1βand IL-6 were increased,and F4/80+CD206+CD86-cells and IL-10 were decreased compared with Mc+HG group and McM group(P0.8).Compered to M group,myotube area,myotube n

关键词： Macrophages phenotype Myoblasts Cross-talk Glucose toxicity Chronic inflammation Insulin sensitivity

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effects of different acupuncture methods combined with routine rehabilitation on gait of stroke patients

引用

World Journal of Clinical Cases 2020年第24期8卷 6282-6295页

作者： Yan-Tao Lou Xi-Cheng Zhen Jing-Jing Yang Yu-Fei Ma Department of Human sports Science Shenyang Sport UniversityShenyang 110102Liaoning ProvinceChina Department of Medical Nursing Jiyuan Vocational and Technical CollegeJiyuan 459000Henan ProvinceChina Department of Rehabilitation Medicine Liaoning Thrombus Treatment Center of Integrated Chinese and Western MedicineShenyang 110102Liaoning ProvinceChina

BACKGROUND Stroke refers to a group of cerebrovascular diseases associated with organic braininjury. It is characterized by the sudden and rapid onset of focal or diffusedysfunction. In recent years, in addition to routine treatment, Chinese medicineacupuncture has been administered to patients with hemiplegia, and it can beconsidered a new treatment for *** To investigate the effects of eye acupuncture needle retention and bodyacupuncture combined with routine rehabilitation on gait performance andplantar pressure in patients recovering from *** Thirty-two stroke patients who met the inclusion criteria were randomly dividedinto an experimental group and a control group, with 16 patients in each *** groups underwent routine rehabilitation. The experimental group wastreated by eye acupuncture needle retention, and the control group was treated bybody acupuncture. Before and after 4 wk of treatment, both groups underwentkinematic and plantar pressure synchronous tests to assess gait *** The step length, gait speed, step frequency, joint angles of the lower limbs, andground reaction force impulse in the anterior region of the affected foot in bothgroups significantly increased from before to after treatment (P center of mass displacement, peak pressure values, and impulse in the anterior region ofthe healthy foot and posterior regions of both the affected and healthy feetsignificantly decreased from before to after treatment (P center of gravity displacement, and peak pressure values andimpulse in the anterior and posterior regions of both the affected and healthy feet(P < 0.05).CONCLUSION Eye acupuncture needle retention and body acupuncture combined with routinerehabilitation can effectively improve the gait perform

关键词： Stroke Eye acupuncture Body acupuncture Gait performance Spasm Patients

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The lymphatic system:a therapeutic target for central nervous system disorders

引用

Neural Regeneration Research 2023年第6期18卷 1249-1256页

作者： Jia-Qi Xu Qian-Qi Liu Sheng-Yuan Huang Chun-Yue Duan Hong-Bin Lu Yong Cao Jian-Zhong Hu Department of Spine Surgery and Orthopaedics Xiangya HospitalCentral South UniversityChangshaHunan ProvinceChina Key Laboratory of Organ Injury Aging and Regenerative Medicine of Hunan ProvinceChangshaHunan ProvinceChina Mobile Health Ministry of Education-China Mobile Joint Laboratory ChangshaHunan ProvinceChina National Clinical Research center for Geriatric Disorders Xiangya HospitalCentral South UniversityChangshaHunan ProvinceChina Department of Cardiovascular Surgery The Second Xiangya HospitalCentral South UniversityChangshaHunan ProvinceChina Department of sports Medicine Xiangya HospitalCentral South UniversityChangshaHunan ProvinceChina

The lymphatic vasculature forms an organized network that covers the whole body and is involved in fluid homeostasis,metabolite clearance,and immune *** recent identification of functional lymphatic vessels in the meninges of the brain and the spinal cord has provided novel insights into *** emerge as major pathways for fluid *** abundance of immune cells in lymphatic vessels and meninges also suggests that lymphatic vessels are actively involved in *** lymphatic system,through its role in the clearance of neurotoxic proteins,autoimmune cell infiltration,and the transmission of pro-inflammatory signals,participates in the pathogenesis of a variety of neurological disorders,including neurodegenerative and neuroinflammatory diseases and traumatic *** endothelial growth factor C is the master regulator of lymphangiogenesis,a process that is critical for the maintenance of central nervous system *** this review,we summarize current knowledge and recent advances relating to the anatomical features and immunological functions of the lymphatic system of the central nervous system and highlight its potential as a therapeutic target for neurological disorders and central nervous system repair.

关键词： central nervous system central nervous system injury glymphatic system lymphatic vessels meninges neurodegenerative disorders neuroinflammatory diseases vascular endothelial growth factor C

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Selection of Reference Genes for Expression Analysis of Kumamoto and Portuguese Oysters and Their Hybrid

引用

Journal of Ocean University of China 2017年第6期16卷 1139-1147页

作者： YAN Lulu SU Jiaqi WANG Zhaoping YAN Xiwu YU Ruihai Fisheries College Ocean University of China South China Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Engineering Research center of Shellfish culture and Breeding of Liaoning province College of Fisheries and Life ScienceDalian Ocean University

Quantitative real-time polymerase chain reaction(q RT-PCR) is a rapid and reliable technique which has been widely used to quantifying gene transcripts(expression analysis). It is also employed for studying heterosis, hybridization breeding and hybrid tolerability of oysters, an ecologically and economically important taxonomic group. For these studies, selection of a suitable set of housekeeping genes as references is crucial for correct interpretation of q RT-PCR data. To identify suitable reference genes for oysters during low temperature and low salinity stresses, we analyzed twelve genes from the gill tissue of Crassostrea sikamea(SS), Crassostrea angulata(AA) and their hybrid(SA), which included three ribosomal genes, 28 S ribosomal protein S5(RPS5), ribosomal protein L35(RPL35), and 60 S ribosomal protein L29(RPL29); three structural genes, tubulin gamma(TUBγ), annexin A6 and A7(AA6 and AA7); three metabolic pathway genes, ornithine decarboxylase(OD), glyceraldehyde-3-phosphate dehydrogenase(GAPDH) and glutathione S-transferase P1(GSP); two transcription factors, elongation factor 1 alpha and beta(EF1α and EF1β); and one protein synthesis gene(ubiquitin(UBQ). Primers specific for these genes were successfully developed for the three groups of oysters. Three different algorithms, ge Norm, Norm Finder and Best Keeper, were used to evaluate the expression stability of these candidate genes. Best Keeper program was found to be the most reliable. Based on our analysis, we found that the expression of RPL35 and EF1α was stable under low salinity stress, and the expression of OD, GAPDH and EF1α was stable under low temperature stress in hybrid(SA) oyster; the expression of RPS5 and GAPDH was stable under low salinity stress, and the expression of RPS5, UBQ, GAPDH was stable under low temperature stress in SS oyster; the expression of RPS5, GAPDH, EF1β and AA7 was stable under low salinity stress, and the expression of RPL35, EF1α, GAPDH and EF1β was stable under low

关键词： Crassostrea sikamea Crassostrea angulata hybrid oyster reference gene quantitative real-time PCR

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Impact of seawater acidification on shell property of the Manila clam Ruditapes philippinarum grown within and without sediment

引用

Journal of Oceanology and Limnology 2020年第1期38卷 236-248页

作者： YUAN Hongmei XU Xian YANG Feng ZHAO Liqiang YAN Xiwu Engineering Research center of Shellfish culture and Breeding of Liaoning province Dalian Ocean UniversityDalian 116023China Ecological Monitoring and Evaluation Division Wuhan SINOECO Ecological Science&Technology Co.Ltd.Wuhan 430080China

Although the impact of ocean acidification on marine bivalves has been previously investigated under mainly controlled laboratory conditions,it is still unclear whether the impact of acidification on sediment-burrowing species differs between those within or without *** order to fill this gap in our knowledge,we compared shell properties of the infaunal Manila clam(Ruditapes philippinarum)exposed to three pH concentrations(7.4,7.7,and 8.0),within and without *** the first experiment(140 d),clams were exposed to seawater in an acidification system without sediment.A decrease in shell weight corresponding to the increase in dissolution rate was observed in the group ofpH 7.4,at which shell color disappeared or *** observations confirmed the changes of the external shell *** the second experiment(170 d),sediment was placed at the bottom of each exposure *** effects were found obvious in shell dissolution rate and shell color in the shell specimens exposed to overlying seawater but not found in the shell specimens exposed to *** the experimental period was longer in the second experiment,shell specimens in the first experiment were more seriously damaged than those in the second experiment under acidic seawater *** results,in relation to the defense function of the shell,show that marine bivalves in burrowing behavior are more adaptable to seawater acidification than those who do not burrow into sediment.

关键词： ocean acidification sediment Manila clam(Ruditapes philippinarum) shell properties

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Identification of Shell Color-Associated Genes Using Mantle Branch-Specific RNA Sequencing of Yellow-Colored Line of Pearl Oyster Pinctada fucata martensii

引用

Journal of Ocean University of China 2024年第4期23卷 1076-1086页

作者： PENG Haiming LIAO Yongshan YANG Chuangye MKUYE Robert DENG Yuewen YUE Chenyang Fisheries College Guangdong Ocean UniversityZhanjiang 524088China Pearl Research Institute Guangdong Ocean UniversityZhanjiang 524088China Guangdong Science and Innovation center for Pearl culture Zhanjiang 524088China Pearl Breeding and Processing Engineering Technology Research center of Guangdong province Zhanjiang 524088China Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy culture Zhanjiang 524088China

The yellow-colored line of pearl oyster Pinctada fucata martensii displays a yellow prismatic layer and a white nacreous layer that can be used as an ideal model for research on shell color ***-Raman spectroscopy and transcriptome analyses were performed to explore the potential molecular mechanism underlying the phenotype *** micro-Raman spectroscopy results indicate that the prismatic layer exhibits distinct characteristic peaks of carotenoids,while these peaks are not prominent in the nacreous *** the transcriptome comparison of the central zone of mantle and mantle edge tissue,which function in nacreous and prismatic layer formation,respectively,935 significantly differentially expressed genes(DEGs)were identified,with 385 genes upregulated and 227 genes downregulated(|log_(2)(Fold change)|>1 and false discovery rate<0.05)in the mantle edge *** these genes,some were associated with melanoma/melanogenesis,such as tyrosinase,zinc metalloprotease,glutathione S-transferase,and ATP-binding cassette sub-family;some were associated with the carotenoid-related pathway,including scavenger receptors,cytochrome P450 and lipoprotein *** associated with porphyrin metabolism,including porphobilinogen deaminase,and copper/zinc superoxide dismutase,and genes associated with shell matrix protein,including amorphous calcium carbonate binding protein,shematrin,PIF,and collagen,also exhibited significantly different *** is speculated that the different colours between prismatic layer and nacreous layer in the yellow-colored line of *** might be resulted from melanin,carotenoids and porphyrin metabolism,while genes related to shell structure and biomineralization might also affect *** results provide new insights to understand the mechanism of shell color formation in mollusca.

关键词： Pinctada fucata martensii shell coloration transcriptome

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Inbreeding Depression on Growth and Survival of Full-Sib Family of Manila Clam(Ruditapes philippinarum)

引用

Journal of Ocean University of China 2017年第1期16卷 145-150页

作者： HUO Zhongming LI Na ZHANG Xuekai LI Ying YAN Xiwu YANG Feng College of Fisheries and Life Science Dalian Ocean UniversityDalian 116023P.R.China Engineering Research center of Shellfish culture and Breeding in Liaoning province Dalian Ocean UniversityDalian 116023P.R.China

In present study, the inbreeding depression(ID) of growth and survival of Manila clam(Ruditapes philippinarum) was investigated at larval and juvenile stages. Nine inbred families(A_2, B_2, C_2, D_2, E_2, F_2, G_2, H_2 and I_2) were established by mating within nine full-sib families with expected inbreeding coefficient of 0.25. Inbred families showed significant differences in shell length and hatching rate of D-larvae(straight-hinged larvae). The larvae of the nine inbred families grew slower than those of control group(CG), and their ID value ranged from 0.81% ± 6.09% to 16.10% ± 1.49%. The ID value of larval survival rate varied between 27.47% ± 9.36% and 70.50% ± 13.66%. The ID was also detected for juvenile growth in A_2, B_2, C_2, and D_2, which ranged from 4.60 ± 2.21 to 17.71 ± 7.73. The A_2 family maintained the highest juvenile survival rate, whereas the other inbred families exhibited ID values varying between 62.79% ± 4.54% and 96.14% ± 0.87%. The linear relationship of estimated ID between growth and survival was negatively correlated(R =-0.434, P < 0.05). The results of this study suggested that the ID of growth was common at the larval stage but was less prevalent at juvenile stage. In contrast, the ID of survival increased from larval to juvenile stage. A better understanding of the effect of inbreeding may aid to selective breeding of Manila clam.

关键词： Ruditapes philippinarum growth survival inbreeding depression

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Bone marrow mesenchymal stem cells and exercise restore motor function following spinal cord injury by activating PI3K/AKT/mTOR pathway

引用

Neural Regeneration Research 2023年第5期18卷 1067-1075页

作者： Xin Sun Li-Yi Huang Hong-Xia Pan Li-Juan Li Lu Wang Gai-Qin Pei Yang Wang Qing Zhang Hong-Xin Cheng Cheng-Qi He Quan Wei Rehabilitation Medical center and Institute of Rehabilitation Medicine West China HospitalSichuan UniversityChengduSichuan ProvinceChina Key Laboratory of Rehabilitation Medicine in Sichuan province ChengduSichuan ProvinceChina

Although many therapeutic interventions have shown promise in treating spinal cord injury, focusing on a single aspect of repair cannot achieve successful and functional regeneration in patients following spinal cord injury. In this study, we applied a combinatorial approach for treating spinal cord injury involving neuroprotection and rehabilitation, exploiting cell transplantation and functional sensorimotor training to promote nerve regeneration and functional recovery. Here, we used a mouse model of thoracic contusive spinal cord injury to investigate whether the combination of bone marrow mesenchymal stem cell transplantation and exercise training has a synergistic effect on functional restoration. Locomotor function was evaluated by the Basso Mouse Scale, horizontal ladder test, and footprint analysis. Magnetic resonance imaging, histological examination, transmission electron microscopy observation, immunofluorescence staining, and western blotting were performed 8 weeks after spinal cord injury to further explore the potential mechanism behind the synergistic repair effect. In vivo, the combination of bone marrow mesenchymal stem cell transplantation and exercise showed a better therapeutic effect on motor function than the single treatments. Further investigations revealed that the combination of bone marrow mesenchymal stem cell transplantation and exercise markedly reduced fibrotic scar tissue, protected neurons, and promoted axon and myelin protection. Additionally, the synergistic effects of bone marrow mesenchymal stem cell transplantation and exercise on spinal cord injury recovery occurred via the PI3 K/AKT/mTOR pathway. In vitro, experimental evidence from the PC12 cell line and primary cortical neuron culture also demonstrated that blocking of the PI3 K/AKT/mTOR pathway would aggravate neuronal damage. Thus, bone marrow mesenchymal stem cell transplantation combined with exercise training can effectively restore motor function after spinal cord inj

关键词： axon growth bone marrow mesenchymal stem cell exercise training mTOR neuroprotection neurotrophin remyelination scar formation spinal cord injury synaptic plasticity

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The role of purinergic receptors in neural repair and regeneration after spinal cord injury

引用

Neural Regeneration Research 2023年第8期18卷 1684-1690页

作者： Rui-Dong Cheng Wen Ren Ben-Yan Luo Xiang-Ming Ye center for Rehabilitation Medicine Rehabilitation&Sports Medicine Research Institute of Zhejiang ProvinceDepartment of Rehabilitation MedicineZhejiang Provincial People’s Hospital(Affiliated People’s HospitalHangzhou Medical College)HangzhouZhejiang ProvinceChina Department of Family Medicine The First Affiliated HospitalZhejiang University School of MedicineHangzhouZhejiang ProvinceChina Department of Neurology&Brain Medical center The First Affiliated HospitalZhejiang University School of MedicineHangzhouZhejiang ProvinceChina

Spinal cord injury is a serious injury of the central nervous system that results in neurological *** pathophysiological mechanisms underlying spinal cord injury,as well as the mechanisms involved in neural repair and regeneration,are highly *** there have been many studies on these mechanisms,there is no effective intervention for such *** spinal cord injury,neural repair and regeneration is an important part of improving neurological function after injury,although the low regenerative ability of nerve cells and the difficulty in axonal and myelin regeneration after spinal cord injury hamper functional *** amounts of ATP and its metabolites are released after spinal cord injury and participate in various aspects of functional regulation by acting on purinergic receptors which are widely expressed in the spinal *** processes mediate intracellular and extracellular signalling pathways to improve neural repair and regeneration after spinal cord *** article reviews research on the mechanistic roles of purinergic receptors in spinal cord injury,highlighting the potential role of purinergic receptors as interventional targets for neural repair and regeneration after spinal cord injury.

关键词： glial cells glial scar inflammatory responses neural regeneration neural repair neural stem cells purinergic receptors spinal cord injury

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A review on anthropogenic geomorphology

引用

Journal of Geographical Sciences 2017年第1期27卷 109-128页

作者： LI Jialin YANG Lei PU Ruiliang LIU Yongchao Department of Geography & Spatial Information Technology Ningbo University Ningbo 315211 Zhejiang China Research center for Marine culture and Economy Zhejiang Province Ningbo 315211 Zhejiang China School of Geosciences University of South Florida Tampa. FL 33620 USA

With the continuous development of man＇s ability to reshape naturehuman activities have become the third geomorphologic agent in the modern geomorphological process. Man-made landform is a landform unit characterized by human activities and is a result of synergizing human and nature geomorphologic agents under the physical geographical background. This article provides an overview on the major progresses in research on anthropogenic geomorphology from aspects like the origin of anthropogenic geomorphologyman-made landform agents and classificationman-made landform evolution and its influencing mechanismmap presentation of man-made landformand environmental impact of man-made landforms. In additionin the articlethe future development of anthropogenic geomorphology is forecasted. It is pointed out that future studies on anthropogenic geomorphology should pay more attention to the following directions： construction of discipline system of anthropogenic geomorphologymaterial composition and morphological features of man-made landformsspatial expansion process and development laws of man-made landformsregional disparity and accumulative environmental effects of man-made landformsand environmental management on man-made landforms and comparative analyses of relevant international management policies.

关键词： anthropogenic geomorphology the third geomorphologic agent man-made landform evolution urban anthropogenic geomorphology man-made landform environmental management

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：