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Traction force with extracellular matrix mediated by cytoskeleton influences metastasis through SLC8A1 induced Wnt-b-catenin pathway in endometrial cancer

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Genes & Diseases 2024年第5期11卷 98-101页

作者： Xingchen Li Xin Xu Jianliu Wang Department of obstetrics and gynecology Peking University People’s HospitalBeijing 100044China Department of obstetrics and gynecology Beijing ChaoYang HospitalCapital Medical UniversityBeijing 100020China

Endometrial cancer(EC)is one of the most prominent malignancies in ***,despite these advancements in understanding EC pathogenesis,mortality rates have not *** force(TF)refers to endogenous mechanical forces exerted by cells.1 These forces act on the extracellular matrix and the extracellular tumor microenvironment through focal adhesions,thereby regulating cell proliferation and ***,the TF characteristics of endometrial cancer cells and their heterogeneity remain unclear.

关键词： endometrial metastasis endogenous

Identifying Comprehensive Genomic Alterations and Potential Neoantigens for Cervical Cancer Immunotherapy in a Cohort of Chinese Squamous Cell Carcinoma of the Cervix

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Biomedical and Environmental Sciences 2024年第6期37卷 565-580页

作者： Meng Wu Jialu Zhou Zhe Zhang Yuanguang Meng School of Medicine Nankai UniversityTianjin 300071China Department of obstetrics and gynecology The First Medical Center of Chinese PLA General HospitalBeijing 100853China Department of obstetrics and gynecology Chinese PLA Medical SchoolBeijing 100853China Department of obstetrics and gynecology The Seventh Medical Center of Chinese PLA General HospitalBeijing 100700China

Objective Genomic alterations and potential neoantigens for cervical cancer immunotherapy were identified in a cohort of Chinese patients with cervical squamous cell carcinoma(CSCC).Methods Whole-exome sequencing was used to identify genomic alterations and potential neoantigens for CSCC *** Sequencing was performed to analyze neoantigen *** Systematic bioinformatics analysis showed that C>T/G>A transitions/transversions were dominant in *** mutations were the most frequent types of somatic mutation in the coding sequence *** signature analysis detected signature 2,signature 6,and signature 7 in CSCC ***3CA,FBXW7,and BICRA were identified as potential driver genes,with BICRA as a newly reported *** variation profiling identified 4,960 potential neoantigens,of which 114 were listed in two neoantigen-related *** The present findings contribute to our understanding of the genomic characteristics of CSCC and provide a foundation for the development of new biotechnology methods for individualized immunotherapy in CSCC.

关键词： Cervical squamous cell carcinoma Genome alteration Neoantigens Immunotherapy

Efficacy and Safety of Sanjie Analgesic Capsule in Patients with Endometriosis-Associated Pain:A Multicenter,3:1 Randomized,Double-Blind,Placebo-Controlled Trial

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Chinese Journal of Integrative Medicine 2024年第9期30卷 780-787页

作者： LENG Jin-hua DUAN Hua GUAN Zheng ZHOU Ying-fang QU Hong XU Kai-hong ZHANG Shao-fen ZHANG Qin WANG Xin LIN Kai-qing LANG Jing-he Department of obstetrics and gynecology Peking Union Medical College HospitalBeijing100730China Department of gynecology Beijing Obstetrics and Gynecology HospitalCapital Medical UniversityBeijing100006China Department of gynecology Chinese PLA General HospitalBeijing100039China Department of gynecology Peking University First HospitalBeijing100034China Department of gynecology Beijing Chao-yang HospitalBeijing100020China Department of gynecology Womens Hospital School of Medicine Zhejiang UniversityHangzhouZhejiang Province310006China Department of gynecology Obstetrics and Gynecology Hospital of Fudan UniversityShanghai200090China Department of gynecology Hangzhou Hospital of Traditional Chinese MedicineHangzhou310007China Department of gynecology Affiliated Hospital of Liaoning University of Traditional Chinese MedicineShenyang110032China

Objective:To assess the efficacy and safety of Sanjie Analgesic Capsule(SAC)in Chinese patients with endometriosis-associated ***:This was a multicenter,randomized,double-blind,placebo-controlled trial conducted at 15 centers between November 2013 and July 2017 in *** 323 patients with endometriosis were randomized at a 3:1 ratio to the SAC group(241 cases)and placebo group(82 cases)by stratified block *** in the SAC or placebo groups were given SAC or placebo 1.6 g 3 times per day,orally,respectively since the first day of menstruation for 3 consecutive menstrual *** primary endpoint was clinical response to dysmenorrhea evaluated using a 10-point Visual Analogue Scale at 3 and 6 *** secondary endpoint was the pain score evaluated by VAS(chronic pelvic pain,defecation pain,and dyspareunia)at 3 and 6 months,and the pain recurrence rate at 6 *** events(AEs)were recorded during the ***:A total of 241 women were included in the SAC group,and 82 were in the placebo *** these women,217(90.0%)and 71(86.6%)completed the intervention,*** 3 months,overall response rate(ORR)was significantly higher in women administered SAC(80.1%)compared with those who received a placebo(30.5%,P<0.01).Six months after treatment,the ORR for dysmenorrhea was 62.7%in the SAC group and 31.7%in the placebo group(P<0.01).Chronic pelvic pain and defecation pain were significantly improved by SAC compared with placebo(both P<0.05).The incidence rates of total AEs events in the SAC and placebo groups were 6.6%and 9.8%,respectively,and no significant difference was shown between the two groups(P=0.339).Conclusion:SAC is well-tolerated and may improve dysmenorrhea in women with endometriosis-associated pain.

关键词： endometriosis pain Sanjie Analgesic Capsule randomized controlled trial Chinese medicine

LncRNA PCGEM1 facilitates cervical cancer progression via miR-642a-5p/KIF5B axis

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Oncology Research 2024年第7期32卷 1221-1229页

作者： YUANLIN LIU YAN LIU YAN WANG QIANG WANG YAN YAN DANDAN ZHANG HUIQIN LIU Department of obstetrics and gynecology Shanghai First Maternity and Infant HospitalSchool of MedicineTongji UniversityShanghai200092China Department of obstetrics and gynecology The Second People’s Hospital of Nantong CityNantong226002China Department of obstetrics and gynecology Affiliated Hospital of Nantong UniversityNantong226002China

At present,the role of many long non-coding RNAs(lncRNAs)as tumor suppressors in the formation and development of cervical cancer(CC)has been ***,lncRNA prostate cancer gene expression marker 1(PCGEM1),whose high expression not only aggravates ovarian cancer but also can induce tumorigenesis and endometrial cancer progression,has not been studied in *** objective of this study was to investigate the expression and the underlying role of PCGEM1 in *** relative expression of PCGEM1 in CC cells was detected by real-time *** the suppression of PCGEM1 expression by shRNA,the changes in the proliferation,migration,and invasion capacities were detected via CCK-8 assay,EdU assay,and colony formation assay wound healing *** assay and the changes in expressions of epithelial-to-mesenchymal transition(EMT)markers were determined by western blot and immunoﬂ*** interplay among PCGEM1,miR-642a-5p,and kinesin family member 5B(KIF5B)was conﬁrmed by bioinformatics analyses and luciferase reporter *** showed that PCGEM1 expressions were up-regulated within CC *** viabilities,migration,and invasion were remarkably reduced after the suppression of PCGEM1 expression by shRNA in Hela and SiHa cells.N-cadherin was silenced,but E-cadherin expression was elevated by ***,by sponging miR-642a-5p in CC,PCGEM1 was veriﬁed as a competitive endogenous RNA(ceRNA)that modulates KIF5B ***-642a-5p down-regulation partially rescued sh-PCGEM1’s inhibitory effects on cell proliferation,migration,invasion,and EMT *** conclusion,the PCGEM1/miR-642a-5p/KIF5B signaling axis might be a novel therapeutic target in *** study provides a research basis and new direction for targeted therapy of CC.

关键词： Cervical cancer PCGEM1 KIF5B MiR-642a-5p tumorigenesis

Exosomes from umbilical cord mesenchymal stromal cells promote the collagen production of fibroblasts from pelvic organ prolapse

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World Journal of Stem Cells 2024年第6期16卷 708-727页

作者： Lei-Mei Xu Xin-Xin Yu Ning Zhang Yi-Song Chen Department of gynecology Obstetrics and Gynecology Hospital of Fudan UniversityShanghai 200011China Department of gynecology Shanghai Key Laboratory of Female Reproductive Endocrine-related DiseasesShanghai 200011China

BACKGROUND Pelvic organ prolapse(POP)involves pelvic organ herniation into the vagina due to pelvic floor tissue laxity,and vaginal structure is an essential *** POP,the vaginal walls exhibit abnormal collagen distribution and decreased fibroblast levels and *** intricate etiology of POP and the prohibition of trans-vaginal meshes in pelvic reconstruction surgery present challenges in targeted therapy *** umbilical cord mesenchymal stromal cells(hucMSCs)present limitations,but their exosomes(hucMSC-Exo)are promising therapeutic tools for promoting fibroblast proliferation and extracellular matrix *** inflammation in POP group fibroblasts,stimulated primary fibroblast growth,and elevated collagen I(Col1)production in ***-throughput RNA-seq of fibroblasts treated with hucMSC-Exo and miRNA sequencing of hucMSC-Exo revealed that abundant exosomal miRNAs downregulated matrix metalloproteinase 11(MMP11)*** HucMSC-Exo normalized the growth and function of primary fibroblasts from patients with POP by promoting cell growth and Col1 expression in *** miRNAs in hucMSC-Exo targeted and downregulated MMP11 ***-Exo-based therapy may be ideal for safely and effectively treating POP.

关键词： Pelvic organ prolapse Exosomes Fibroblasts Human umbilical cord mesenchymal stromal cells Extracellular matrix Collagen I

Subsequent pregnancy outcomes and fertility rates in the case series that underwent bilateral hypogastric artery ligation(BHGAL)due to severe postpartum hemorrhage

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Asian pacific Journal of Reproduction 2024年第1期13卷 28-33页

作者： OKUTUCU Gulcan EVSEN Mehmet S PEKER Nurullah YAMAN TUNC Senem ICEN Mehmet S Department of obstetrics and gynecology Division of PerinatologyTurkish Ministry of Health Ankara City HospitalAnkaraTurkey Department of obstetrics and gynecology Dicle UniversitySchool of MedicineDiyarbakirTurkey

Objective:To evaluate the effect of bilateral hypogastric artery ligation(BHGAL)on women's pregnancy outcomes and fertility rates in cases with severe postpartum ***:Patients hospitalized in a tertiary care center between 2007 and 2018 were included in the *** records of cases were obtained from the hospital database,*** demographic data,morbidities or complications associated with BHGAL surgery,any additional haemostatic interventional or surgical procedures,preoperative admission and lowest postoperative haematocrit-hemoglobin values,blood products given,type of anaesthesia,intensive care unit(ICU)and length of hospital stay were *** cases without surgical sterilization,future fertility and subsequent pregnancy outcomes were assessed for at least two years after *** was obtained through face-to-face or telephone interviews with patients whose long-term fertility and subsequent pregnancy outcomes were ***:Sixty-eight patients with BHGAL surgery history were included in the study.26 Of 37 cases(70%)that did not undergo surgical sterilization had a desire for pregnancy after the *** of these cases(23%)were diagnosed with secondary *** fertility rate of these cases was 77%,and subsequent pregnancy was detected in 20 women.A total of 25 pregnancies,including first and second pregnancies,developed,and 15 of these pregnancies resulted in term delivery,six preterm births,one stillbirth,and three ***:The postoperative pregnancy rate was found to be higher than the rate of women with infertility problems,and this surgery does not seem to adversely affect pregnancy *** is a tertiary center experience and cohort studies with large patient series are needed.

关键词： Fertility Iliac artery Ligations Postpartum hemorrhage Pregnancy outcomes

Ultra-conservative noncoding RNA uc.243 confers chemo-resistance by facilitating the efflux of the chemotherapeutic drug in ovarian cancer

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BIOCELL 2024年第8期48卷 1265-1273页

作者： SHAN JIANG XIUFENG LIN YANFEI CHEN XINNING LI JIALI KANG Department of gynecology and obstetrics The First Affiliated Hospital of Jinan UniversityGuangzhou510180China Reproductive Medical Center Boai Hospital of Zhongshan Affiliated to Southern Medical UniversityZhongshan528400China Department of gynecology and obstetrics Boai Hospital of Zhongshan Affiliated to Southern Medical UniversityZhongshan528400China Department of gynecology and obstetrics Guangzhou First People’s HospitalGuangzhou Medical UniversityGuangzhou510180China

Background:Despite improvements in objective response rates to cisplatin-based combination chemotherapy,the majority of advanced ovarian cancer remains suboptimal,resulting in poor *** has been found that non-coding RNAs(ncRNAs)not only participate in the transmission of signals between various cells but also participate in tumor immunity and anti-tumor immune responses,thereby regulating tumor occurrence and ***,the function and detailed mechanism of ultraconserved RNA(ucRNA)in ovarian cancer chemoresistance is still ***:Western blotting assay,Quantitative real-time PCR analysis(qPCR),and Kaplan-Meier Plotter analysis were performed to analyze the expression and prognosis of uc.243 in ovarian *** assay and Annexin V assay were performed to analyze the function of uc.243 in cisplatin resistance in ovarian cancer *** pull-down and qPCR experiments were performed to explore the molecular mechanism of uc.243 enhancing cisplatin resistance in ovarian cancer ***:Herein,we found that uc.243 was remarkably upregulated and correlated with patient survival in chemoresistance ovarian cancer patients compared with chemo-sensitive ovarian *** experiment displayed that uc.243 induced cisplatin resistance on ovarian cancer cells by facilitating the efflux of cisplatin(CDDP);but inhibiting the expression of uc.243 significantly reverses this ***,uc.243 can inhibit the binding of RNA binding protein DGCR8 microprocessor complex subunit to pri-miR-155,thereby inhibiting the cleavage of pri-miR-155 and decrease in mature miR-155,subsequently upregulates the expression of ATP binding cassette subfamily B member(ABCB1,ABCC2).Conclusion:Our research findings indicate that uc.243 can induce chemotherapy resistance in ovarian cancer,suggesting that it may become a new prognostic biomarker for malignant ovarian cancer.

关键词： Ultra-conserved non-coding RNA uc.243 Drug efflux Drug transporters Chemoresistance Ovarian cancer

Knockdown of circular RNA (CircRNA)_001896 inhibits cervical cancer proliferation and stemness in vivo and in vitro

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BIOCELL 2024年第4期48卷 571-580页

作者： JIA SHAO CAN ZHANG YAONAN TANG AIQIN HE WEIPEI ZHU Department of obstetrics and gynecology The Second Affiliated Hospital of Soochow UniversitySuzhou215004China Department of gynecology Oncology Affiliated Tumor Hospital of Nantong UniversityNantong226361China

Objective:Previous studies indicated that aberrant circular RNA(circRNA)expression affects gene expression regulatory networks,leading to the aberrant activation of tumor pathways and promoting tumor cell ***,the expression,clinical signiﬁcance,and effects on cell propagation,invasion,and dissemination of circRNA_001896 in cervical cancer(CC)tissues remain ***:The Gene Expression Omnibus(GEO)datasets(GSE113696 and GSE102686)were used to examine differential circRNA expression in CC and adjacent *** expression of circRNA_001896 was detected in 72 CC patients usingﬂuorescence quantitative *** analysis with clinical pathological features was performed through COX multivariate and univariate *** effect of circRNA_001896 downregulation on CC cell propagation was examined using the cell counting kit-8(CCK-8)test,clonogenic,3D sphere formation,and in vivo tumorigenesis ***:Intersection of the GSE113696 and GSE102686 datasets revealed an increased expression of four circRNAs,including circRNA_001896,in CC *** quantitative PCR conﬁrmed circRNA_001896 as a circular *** expression of circRNA_001896 was considerably associated with lymph node metastasis,International Federation of Gynecologists and Obstetricians(FIGO)stage,tumor diameter,and survival period in CC *** hazards model(COX)univariate and multivariate analyses revealed that circRNA_001896 expressions are a distinct risk factor affecting CC patients’*** functional experiments showed that downregulating circRNA_001896 substantially suppressed CC cell growth,colony formation,and 3D sphere-forming *** vivo,tumorigenesis analysis in nude mice demonstrated that downregulating circRNA_001896 remarkably reduced the in vivo proliferation capacity of CC ***:CircRNA_001896 is highly expressed in CC tissues and is substantially related to lymph node metastasis,FIGO stage,tumor size,and surviva

关键词： Uterine cervical neoplasms RNA Circular Cell proliferation Cancer stem cells

Histological Features of Uterine Myometrial Dysfunction:Possible Involvement of Localized Inflammation

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Current Medical Science 2024年第3期44卷 633-641页

作者： Sheng-lan ZHU Hui-ting ZHANG Yuan-yuan DU Yi JIANG Shao-shuai WANG Wen-cheng DING Ling FENG National Clinical Research Center for obstetrics and gynecology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China Department of obstetrics and gynecology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China

Objective The latest perspective suggests that elevated levels of inflammation and cytokines are implicated in atonic postpartum ***(LPS)has been widely used to induce inflammation in animal ***,this study aimed to induce uterine inflammation using LPS to investigate whether local inflammation triggers dysfunction and atrophy in the myometrium,as well as the potential underlying molecular mechanisms *** In vivo,an animal model was established by intraperitoneal injection of 300μg/kg LPS in rats on gestational day ***-eosin(H&E)staining and Masson staining were employed to determine morphological changes in the rat uterine smooth ***-linked immunosorbent assay(ELISA)was used to detect inflammatory ***,tissue fluorescence,and Western blotting were conducted to assess the expression levels of the uterine contraction-related proteins Toll-like receptor 4(TLR4)and the nuclear factor kappa-B(NF-κB)signaling *** vitro,human uterine smooth muscle cells(HUtSMCs)were exposed to 2μg/mL LPS to further elucidate the involvement of the TLR4/NF-κB signaling pathway in LPS-mediated *** In this study,LPS induced uterine myometrial dysfunction in rats,leading to a disorganized arrangement,a significant increase in collagen fiber deposition,and widespread infiltration of inflammatory *** both in vivo animal models and in vitro HUtSMCs,LPS elevated IL-6,IL-1β,and TNF-αlevels while concurrently suppressing the expression of connexin 43(Cx43)and oxytocin receptor(OXTR).Mechanistically,the LPS-treated group exhibited TLR4 activation,and the phosphorylation levels of p65 and IκBαwere notably *** LPS triggered the TLR4/NF-κB signaling pathway,inducing an inflammatory response in the myometrium and leading to uterine myometrial dysfunction and uterine atony.

关键词： lipopolysaccharide inflammatory response TLR4 NF-κB myometrium