检索结果-南通市图书馆

Protein kinase clk/STY is differentially regulated during erythroleukemia cell differentiation: a bias toward the skipped splice variant characterizes postcommitment stages

引用

Cell Research 2005年第7期15卷 495-503页

作者： Ana GARCíA-SACRISTáN■,María J. FERNáNdeZ-NESTOSA,Pablo HERNáNdeZ,Jorge B.SCHVARTZMAN,Dora B. KRIMER department of Cell and developmental Biology Centro de Investigaciones Biológicas consejo superior de investigacionescientíficas Ramiro de Maeztu 9 Madrid 28040 Spain Instiute of Molecular Medicine 1649-028 Lisbon Medical School Lisbon Portugal department of Cell and developmental Biology Centro de Investigaciones Biológicas consejo superior de investigacionescientíficas Ramiro de Maeztu 9 Madrid 28040 Spain department of Cell and developmental Biology Centro de Investigaciones Biológicas consejo superior de investigacionescientíficas Ramiro de Maeztu 9 Madrid 28040 Spain department of Cell and developmental Biology Centro de Investigaciones Biológicas consejo superior de investigacionescientíficas Ramiro de Maeztu 9 Madrid 28040 Spain department of Cell and developmental Biology Centro de Investigaciones Biológicas consejo superior de investigacionescientíficas Ramiro de Maeztu 9 Madrid 28040 Spain

Clk/STY is a LAMMER protein kinase capable to phosphorylate serine/arginine-rich (SR) proteins that modulate pre-mRNA splicing. Clk/STY alternative splicing generates transcripts encoding a full-length kinase and a truncated catalyti-cally inactive protein. Here we showed that clk/STY, as well as other members of the family (e.g. clk2, clk3 and clk4),are up-regulated during HMBA-induced erythroleukemia cell differentiation. mRNAs coding for the full-length and thetruncated forms were responsible for the overall increased expression. In clk/STY, however, a switch was observed forthe ratio of the two alternative spliced products. In undifferentiated cells the full-length transcript was more abundantwhereas the transcript encoding for the truncated form predominated at latter stages of differentiation. Surprisingly,overexpression of clk/STY did not alter the splicing switch upon differentiation in MEL cells. These results suggest thatclk/STY might contribute to control erythroid differentiation by a mechanism that implicates a balance between thesetwo isoforms.

关键词： clk/STY LAMMER kinase alternative splicing erythroleukemia cells.

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：