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抑郁症患者药物治疗前后单核细胞中microRNA表达水平变化与抑郁症状的关系

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解放军医学杂志 2015年第2期40卷 128-132页

作者：张巧丽过伟张理义赵林范惠民钟爱芳牛威戴运华孙欣羊卢正斌陈升东宋红涛徐州医学院研究生学院精神卫生系江苏徐州221003 解放军102医院全军心理疾病防治中心江苏常州213003 第二军医大学临床医学院精神卫生系上海200433 GoPath laboratories LLC Illinois USA60637 江苏大学临床医学院精神卫生系江苏镇江212013

目的探讨抑郁症患者药物治疗前后单核细胞中micro RNA(mi RNA)的表达水平与抑郁症状的关系。方法纳入2012年8月-2013年10月解放军102医院收治的81例未经药物治疗、符合DSM-Ⅳ诊断标准的抑郁症患者作为病例组,纳入同期81例正常人作为对... 详细信息

目的探讨抑郁症患者药物治疗前后单核细胞中micro RNA(mi RNA)的表达水平与抑郁症状的关系。方法纳入2012年8月-2013年10月解放军102医院收治的81例未经药物治疗、符合DSM-Ⅳ诊断标准的抑郁症患者作为病例组,纳入同期81例正常人作为对照组。抽取3例患者和3例正常人,采用基因芯片筛查出26种mi RNA,然后以实时荧光定量PCR技术检测两组单核细胞中9种mi RNA(mi R-146b、mi R-1972、mi R-26b、mi R-29b、mi R-338、mi R-4485、mi R-4498、mi R-4743和mi R-874)的表达水平。选取病例组中20例患者,分别在用药前和用药(包括文拉法辛、舍曲林、米氮平等)6周后检测mi RNA表达水平,并采用汉密顿抑郁量表(HAMD)和临床疗效总评量表(CGI)对治疗前及治疗6周后的临床症状及疗效进行评估。结果与对照组比较,病例组单核细胞中5种mi RNA(mi R-26b、mi R-4743、mi R-4498、mi R-4485、mi R-1972)表达上调,差异有统计学意义(P<0.05)。抗抑郁药治疗前后,Δmi RNA-1972、Δmi RNA-4485、Δmi RNA-4498、Δmi RNA-4743与阻滞因子的改善程度呈正相关(P<0.05),Δmi RNA-26b与日夜变化因子的改善程度呈负相关(P<0.05)。Logistic回归分析显示,mi RNA-4485能解释阻滞因子变异量的28.8%(P<0.05)。结论抑郁症患者单核细胞中mi R-26b、mi R-4743、mi R-4498、mi R-4485和mi R-1972可能作为反映抑郁症转归的生物标记物。

关键词：抑郁症微RNAs 生物学标记

Scalable and automated CRISPR-based strain engineering using droplet microfluidics

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Microsystems & Nanoengineering 2022年第2期8卷 227-236页

作者： Kosuke Iwai Maren Wehrs Megan Garber Jess Sustarich Lauren Washburn Zachary Costello Peter W.Kim David Ando William R.Gaillard Nathan J.Hillson Paul D.Adams Aindrila Mukhopadhyay Hector Garcia Martin Anup K.Singh Technology Division DOE Joint BioEnergy InstituteEmeryvilleCA 94608USA Biotechnology and Bioengineering Department Sandia National LaboratoriesLivermoreCA 94550USA Biofuels and Bioproducts Division DOE Joint BioEnergy InstituteEmeryvilleCA 94608USA Biological Systems and Engineering Division Lawrence Berkeley National LaboratoryBerkeleyCA 94720USA Molecular Biophysics and Integrated Bioimaging Division Lawrence Berkeley National LaboratoryBerkeleyCA 94720USA Department of Bioengineering University of CaliforniaBerkeleyCA 94720USA BCAM Basque Center for Applied MathematicsBilbao 48009Spain

We present a droplet-based microfluidic system that enables CRISPR-based gene editing and high-throughput screening on a *** microfluidic device contains a 10x10 element array,and each element contains sets of electrodes for two electric field-actuated operations:electrowetting for merging droplets to mix reagents and electroporation for *** device can perform up to 100 genetic modification reactions in parallel,providing a scalable platform for generating the large number of engineered strains required for the combinatorial optimization of genetic pathways and predictable *** demonstrate the system's capabilities through the CRISPR-based engineering of two test cases:(1)disruption of the function of the enzyme galactokinase(galK)in£coli and(2)targeted engineering of the glutamine synthetase gene(glnA)and the blue-pigment synthetase gene(bpsA)to improve indigoidine production in£coli.

关键词： operations generating fluid

Efficacy and safety of tadalafil taken as needed for the treatment of erectile dysfunction in Asian men： results of an integrated analysis

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Asian Journal of Andrology 2009年第4期11卷 423-433页

作者： Ying-Lu Guo Vijay P Viswanathan Han-Sun Chiang Hyung Ki Choi Andrew Wai Chun Yip Wei Shen Vladimir Kopernicky Andrology Center Peking University First Hospital Peking University Beo＇ing 100009 China M. V. Hospital for Diabetes and Diabetes Research Centre Chennai 600013 India School of Medicine Fu Jen Catholic University Taipei 24205 Taiwan China Department of Urology Yongdong Severance Hospital Seoul 135720 Korea Department of Surgery Kwong Wah Hospital Hong Kong China Lilly USA LLC Indianapolis IN 46285 USA

We evaluated the efficacy and safety of as-needed tadalafil in a diverse clinical population （with varying patient demographics, disease severity, and comorbid medical conditions） of Asian men with erectile dysfunction （ED）. An integrated analysis of five double-blind, placebo-controlled trials （N = 1 046） was performed. Patients were randomly assigned to receive 10 mg tadalafil （N = 185）, 20 mg tadalafil （N = 510）, or placebo （N = 351）. Efficacy assessments included the International Index of Erectile Function （IIEF）, Sexual Encounter Profile （SEP） diary and Global Assessment Question （GAQ）. Patients receiving 10 mg or 20 mg tadalafil showed significant improvement from baseline-to-end point on the Erectile Function domain of the International Index of Erectile Function （IIEF-EF） domain score in all clinical sub-populations analyzed, compared with patients receiving placebo （P 〈 0.001）. The 10-mg and 20-mg tadalafil groups showed a mean success rate of 64.1% and 70.5% for sexual intercourse attempts （SEP3, successful intercourse）, respectively, compared with 33.4% in the placebo group （P 〈 0.001）, and 85.5% and 85.4% reported improved erections at end point GAQ, respectively, versus 43.5% in the placebo group （P 〈 0.001）. Tadalafil was well tolerated across all groups studied. Headache and back pain were the most frequently reported adverse events. Overall, tadalafil was effective and well tolerated across a diverse clinical spectrum of Asian men with ED.

关键词： Asia comorbidity erectile dysfunction phosphodiesterase inhibitors tadalafil

精神分裂症患者外周血microRNA表达水平在抗精神病药物治疗前后的变化

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解放军医学杂志 2014年第12期39卷 969-974页

作者：孙欣羊宋红涛赵林仲爱芳牛威张理义卢正斌戴运华解放军102医院全军心理疾病防治中心江苏常州213003 解放军102医院检验科江苏常州213003 解放军102医院康复科江苏常州213003 蚌埠医学院精神医学系安徽蚌埠233030 苏州广济医院精神科江苏苏州215008 GoPath laboratories llc 1351 Barclay Blvd Buffalo Grove IL 60089 USA233030 GoPath Diagnostic Laboratory Co. Ltd No.801 Changwuzhonglu Changzhou Jiangsu 213164 China

目的观察抗精神病药物治疗前后精神分裂症患者外周血部分micro RNA(mi RNA)表达水平的变化。方法选择2012年7月-2013年5月解放军102医院连续收治的61例精神分裂症患者(病例组)和62例正常对照(对照组),采用实时定量荧光PCR检测两组血浆中... 详细信息

目的观察抗精神病药物治疗前后精神分裂症患者外周血部分micro RNA(mi RNA)表达水平的变化。方法选择2012年7月-2013年5月解放军102医院连续收治的61例精神分裂症患者(病例组)和62例正常对照(对照组),采用实时定量荧光PCR检测两组血浆中9种mi RNA(mi R-181b、mi R-195、mi R-132、mi R-212、mi R-30e、mi R-346、mi R-34a、mi R-432、mi R-7)的相对表达水平。选其病例组中阳性与阴性症状量表(PANSS)总分>70分的25例患者,分别在用药前、用药(包括奥氮平、奎硫平、齐拉西酮和利培酮)3周和用药6周时重复检测mi RNA表达水平,并采用PANSS、大体评定量表(GAS)和临床疗效总评量表(CGI)对不同治疗阶段的临床症状及疗效进行评估。结果与对照组比较,病例组血浆mi R-181b、mi R-30e、mi R-346、mi R-34a和mi R-7表达水平显著增高(P0.05)。用药过程中,25例患者血浆mi R-132、mi R-195、mi R-30e和mi R-432表达水平与PANSS总分及GAS得分均呈显著相关(P<0.05)。结论 mi R-181b、mi R-132、mi R-30e和mi R-432可能作为预测精神分裂症疾病转归的联合生物标记物。

关键词：精神分裂症微RNAs 抗精神病药生物学标记

Genetics of canine behavior: A review

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World Journal of Medical Genetics 2014年第3期4卷 46-57页

作者： Amanda Rigterink Indiana Veterinary Behavior Services LLCWest LafayetteIN 47906United StatesKatherine HouptCollege of Veterinary MedicineDepartment of Clinical Sciences Cornell UniversityCVM Cornell UniversityIthacaNY 14853-6401United States

The past decade has seen rapid progress in the field of canid behavioral genetics. The recent advances are summarized in this review. The identification of the genes responsible for tameness in silver foxes is the culmination of a half century of behavioral testing and,more recently, genomic investigation. There is agreement that domestic dogs evolved from wolves, but when and from which population remains *** genetic differences between wolves and dogs identified include those for neurotransmitters and *** differences in behavior are well known, but only recently have the genetics underlying these differences been investigated. The genes responsible for flank sucking in Doberman Pinschers and for several other obsessive compulsive problems in other breeds have been identified. Aggression is the least desirable canine trait, and several laboratories have detected differences in neurotransmitters and their receptors between aggressive and non-aggressive dogs. In English Cocker Spaniels, the genes linked to aggressive behavior code for dopamine, serotonin, and glutamate receptors. A dopamine transporter gene has been associated with impulsive behavior in Malinois.

关键词： Dog Wolf Fox Canine aggression Dopamine Serotonin Canid

Exosomes secreted from cardiomyocytes suppress the sensitivity of tumor ferroptosis in ischemic heart failure

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Signal Transduction and Targeted Therapy 2023年第4期8卷 2007-2021页

作者： Ye Yuan Zhongting Mei Zhezhe Qu Guanghui Li Shuting Yu Yingqi Liu Kuiwu Liu Zhihua Shen Jiaying Pu Yanquan Wang Changhao Wang Zhiyong Sun Qian Liu Xiaochen Pang Ao Wang Zijing Ren Tong Wang Ying Liu Jinhuan Hong Jiajie Xie Xin Li Zhonghua Wang Weijie Du Baofeng Yang Department of Pharmacology(The State-Province Key laboratories of Biomedicine Pharmaceutics of China Key Laboratory of Cardiovascular ResearchMinistry of Education)College of PharmacyHarbin Medical UniversityHarbinChina Department of Pharmacy(The University Key Laboratory of Drug Research Heilongjiang Province)The Second Affiliated Hospital of Harbin Medical UniversityHarbinChina Research Unit of Noninfectious Chronic Diseases in Frigid Zone Chinese Academy of Medical Sciences2019RU070 HarbinChina Department of Clinical Pharmacology College of PharmacyHarbin Medical UniversityHarbinChina Department of Cardiology The First Affiliated Hospital of Harbin Medical UniversityHarbinChina Translational Medicine Research and Cooperation Center of Northern China Heilongjiang Academy of Medical SciencesHarbinChina

Heart failure(HF)patients in general have a higher risk of developing *** animal studies have indicated that cardiac remodeling and HF remarkably accelerate tumor progression,highlighting a cause-and-effect relationship between these two disease *** ferroptosis,a prevailing form of non-apoptotic cell death,has been considered a promising therapeutic strategy for human *** critically contribute to proximal and distant organ-organ communications and play crucial roles in regulating diseases in a paracrine ***,whether exosomes control the sensitivity of cancer to ferroptosis via regulating the cardiomyocyte-tumor cell crosstalk in ischemic HF has not yet been ***,we demonstrate that myocardial infarction(MI)decreased the sensitivity of cancer cells to the canonical ferroptosis activator erastin or imidazole ketone erastin in a mouse model of xenograft ***-MI plasma exosomes potently blunted the sensitivity of tumor cells to ferroptosis inducers both in vitro in mouse Lewis lung carcinoma cell line llc and osteosarcoma cell line K7M2 and in vivo with xenograft tumorigenesis *** expression of miR-22-3p in cardiomyocytes and plasma-exosomes was significantly upregulated in the failing hearts of mice with chronic MI and of HF patients as *** of tumor cells with the exosomes isolated from post-MI mouse plasma or overexpression of miR-22-3p alone abrogated erastin-induced ferroptotic cell death in ***-enriched miR-22-3p was packaged in exosomes and transferred into tumor *** of cardiomyocyte-specific miR-22-3p by AAV9 sponge increased the sensitivity of cancer cells to ***4,a pro-ferroptotic gene,was experimentally established as a target of miR-22-3p in tumor *** together,our findings uncovered for the first time that MI suppresses erastin-induced ferroptosis through releasing miR-22-3p-enriched exosomes derived from ***

关键词： Exosomes enriched prevailing

多功能光谱仪测量光致发光光谱在彩色宝石鉴定的应用

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多功能光谱仪测量光致发光光谱在彩色宝石鉴定的应用

2015中国珠宝首饰学术交流会

作者：刘严陆太进兰延 Liu Research laboratories llc 国土资源部珠宝玉石首饰管理中心北京珠宝研究所国土资源部珠宝玉石首饰管理中心深圳珠宝研究所

光致发光测量可以获得有色宝石和钻石的许多特殊的光谱特征,这些特殊的光致发光光谱特征可以用于快速准确鉴定彩色宝石,无色钻石和彩色钻石。专利的多功能光谱仪具有光致发光测量的放大功能和光谱处理功能,可以在室温下快速测量钻石和... 详细信息

光致发光测量可以获得有色宝石和钻石的许多特殊的光谱特征,这些特殊的光致发光光谱特征可以用于快速准确鉴定彩色宝石,无色钻石和彩色钻石。专利的多功能光谱仪具有光致发光测量的放大功能和光谱处理功能,可以在室温下快速测量钻石和彩色宝石的光致发光光谱。经高达一千倍的放大,多功能光谱仪可以测量非常微弱的彩色宝石的光致发光特征光谱峰。通过测量翡翠的光致发光光谱,可以快速准确鉴别B类翡翠。其它彩色宝石也可以用光致发光光谱鉴定,例如红宝石和尖晶石可以很容易地由其光致发光光谱加以分辨。

关键词：光致发光光谱光谱仪宝石 B类翡翠红宝石祖母绿尖晶石

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cnki会议

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PC/ABS合金用磷酸酯阻燃剂应用进展

塑料助剂 2006年第2期 18-22页

作者：唐煌 Sergei V.Levchik 美国旭瑞达有限公司上海代表处 Supresta llc 430 Saw Mill River Rd.ArdsleyNY10502

概述了国外用于PC/ABS合金的磷酸酯阻燃剂单磷酸酯、双磷酸酯和磷酸胺的现状与研究进展。近年来许多跨国公司在PC/ABS合金的阻燃剂上投入大量的研究工作。近期发表的关于PC/ABS合金阻燃剂的专利数目大大超过了任何一种聚合物阻燃剂的专... 详细信息

概述了国外用于PC/ABS合金的磷酸酯阻燃剂单磷酸酯、双磷酸酯和磷酸胺的现状与研究进展。近年来许多跨国公司在PC/ABS合金的阻燃剂上投入大量的研究工作。近期发表的关于PC/ABS合金阻燃剂的专利数目大大超过了任何一种聚合物阻燃剂的专利数量。由于聚碳酸酯是燃烧成炭率很高的聚合物,以固相作用为主的阻燃剂尤其是磷酸酯成为PC/ABS合金阻燃剂的最佳选择。

关键词：磷酸酯阻燃剂 PC/ABS合金