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Investigation of the mesospheric temperature over Fort Collins region

Science China(Technological Sciences) 2014年第8期57卷 1562-1567页

作者： FAN Xin WENG LiBin ZHANG JianBin FANG HanXian XIE YanQiong Institute of Meteorology and Ocean PLA University of Science and Technology sate Key Laboratory of Space Weather Chinese Academy of Sciences Unit 63655 of PLA

The nightly mean mesospheric temperature profiles between 80 and 107 km, observed by Na lidar, over Fort Collins, colorado （41°N, 105°W） from 1990 to 2010, are employed to research the temporal and spatial variations and mesopause. We find that the maximum mean temperature is in summer months above 95 kin, but reverse below 95 kin, and there is a cooler region below 185 K around 97 km in August. The largest seasonal variation is 39.2 K at 81 kin, and the minimum is 6.5 K at 96.5 km. The maximum standard derivation in spring and autumn months are larger than other seasons above 105 kin, but the temperatures in March, June and September are lower than the other months between 82 km and 100 km where winter is the largest season. Moreover, the seasonal variations of the temperature are about 36, 8 and 21 K at 85, 95 and 105 km, respectively, winter is colder and summer is warmer above 97.5 km, but reverse below 92 km. The mesopause height is around 102 km in winter, but 84 km in summer, and the mean speed of decreasing or increasing of the mesopause height is about 5 km/month in spring and autumn months which are about 90 km. The lasting time of the mesopause in winter is near 6 months, longer than other seasons, and the mesopause temperature is about 165 K in cool summer, and 185 K in warm winter.

关键词： Na lidar mesospheric temperature mesopause

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Agrobacterium Tumefaciens Based Transformation of Pelargonium x Hortrum cv. ‘Samba’ with Anti-1-aminocyclopropane-1-carboxylate Synthase cDNA to Regulate Ethylene Biosynthesis

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Engineering（科研） 2012年第10期4卷 4-7页

作者： Rajinder S. Ranu Jianguo Fan Sarada Krishnan Pradeep Agarwal. Amitva Mitra colorado sate university Laboratory of Plant Molecular Biology/Biotechnology Fort Collins CO USA 80523. Department of Plant Pathology The university of Nebraska Lincolin NE USA 68583-0722

Phytohormone, ethylene plays an important role in plant growth and development including fruit ripening and flower senescence. The synthesis of 1-aminocylo-propane-1- carboxylate (ACC), the immediate precursor of ethylene, from S-adenosyl-methionine is catalyzed by ACC synthase;and which is also a rate limiting step in the ethylene biosynthetic pathway. Therefore, it plays a key role in ethylene biosynthesis and the genes that code for ACC synthase are of special interest. Moreover, in zonal geraniums, ethylene bursts released from cuttings can have profound impact on the viability of explants for plant propagation. Biotechnological approach involving genetic modification that may reduce ethylene levels has potential for increasing the shelf-life of cuttings for plant propagation. These considerations have led us to clone several cDNA of ACC synthase genes from Pelargonium x hortorum cv. ?‘Sincerity’. To transform geranium cells with Agrobacterium tumefaciens an in vitro regeneration? system was developed using very young petiole explants. An Antisense construct of ACC synthase cDNA (PHSacc41) ligated into binary vector pAM696 was introduced into A. tumefaciens EHA 105 cells. Petiole explants were incubated with the Agrobacterium for 15 min and then co-cultivated for several days on MS medium containing 5 mM BAP and ?1 mM IAA in? the dark without the antibiotics. Selection for transformants was carried out in the presence of kanamycin and timentin. Transgenic plantlets generated were examined for inserted gene cassette by PCR and Southern blotting. Recovery of positive transformants that survived selection suggested that it is possible to transform and introduce genes via transformation in hybrid geraniums for genetic modification.

关键词： ACC Synthase Ethylene Biosynthesis Transformation Geranium Regeneration Agrobacterium Tumefaciens

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