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Preparation of Anti-Idiotypic Antibody against Avian Influenza Virus Subtype H9

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Cellular & Molecular Immunology 2005年第2期2卷 155-157页

作者： BaoquanLi JunPeng ZhongxiangNiu XunheYin FaxiaoLiu collegeofanimalscienceandtechnology ShandongAgriculturalUniversityTai＇anChina collegeofanimalscienceandtechnology ShandongAgricultural／／Correspondingto：Dr.ZhongxiangNiuCollegeofAnimalScienceandTechnologyShandongAgriculturalUniversityTai＇an271018China.

To generate monoclonal anti-idiotypic antibodies(mAb2)against avian influenza virus subtype H9(H9 AⅣ), BALB/c mice were immunized with purified chicken anti-H9-AⅣ IgG and the splenocytes of immunized mice were fused with myeloma cells *** cells were screened by indirect enzyme-linked immunosorbent assays with both chicken and rabbit anti-H9-AⅣ IgG as coating *** hybridoma cell clone secreting monoclonal antibody against idiotypes shared by both chicken and rabbit anti-H9-AⅣ IgG was *** demonstrated the mAb2 was able to inhibit the binding of hemagglutinin to anti-H9-AⅣ IgG and to induce chickens to generate hemagglutination inhibition antibodies,indicating this anti-species-sharing-idiotypic antibody bore the internal image of hemagglutinin on avian influenza *** & Molecular Immunology.2005;2(2): 155-157.

关键词： avian influenza virus anti-idiotypic antibody idiotype

Single nucleotide polymorphism analysis in chicken growth hormone gene and its associations with growth and carcass traits

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Chinese Science Bulletin 2003年第15期48卷 1561-1564页

作者： YANBingxue DENGXuemei FEIJing HUXiaoxiang WUChangxin LINing StateKeyLaboratoriesofAgrobiotechnology collegeofanimalscienceandtechnology ChinaAgriculturalUniversityBeijing100094China

In this experiment, F2 chicken derived from Broilers crossing to Silky are used to study the effect of growth hormone gene on growth and carcass traits. The partial gene is amplified by two pairs of primers, and single nucleotide polymorphism (SNPs) is detected by the technique of PCR-RFLP (restriction fragment length polymorphism), and then confirmed by DNA sequencing. The mutations are found in intron 3 and intron 4 respectively, and can be clarified by digestion withEcoR V andMsp I. The results of least square analysis indicate that the gene has significant association with some carcass traits, such as breast muscle weight, breast muscle rate, abdominal fat rate, and has no association with other growth and carcass traits, such as live weight, carcass weight, eviscerated yield with giblet, eviscerated yield, leg muscle weight, heart weight, liver weight, abdominal fat weight, chest angle width, head and neck weight, shank and claw weight, wing weight, muscular stomach weight, glandular stomach weight, ovary or testicular weight, shank girth, small intestine length, 1-week body weight, 6-week body weight, 12-week body weight, etc. These results demonstrate that GH gene could be a genetic locus or linked to a major gene significantly affecting the growth and carcass traits in chicken.

关键词：核苷酸多态现象激素鸡养殖技术

Quantitative traits correlative analysis and growth comparison among different populations of bay scallop,Argopecten irradians

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Acta Oceanologica Sinica 2004年第3期23卷 533-540页

作者： LIUBaozhong LIANGYubo LIUXiaolin DONGBo XIANGJianhai InstituteofOceanology ChineseAcademyofSciencesQingdao266071China NationalMarineEnvironmentMonitoringCenter Dalian116023China collegeofanimalscienceandtechnology NorthwestSci-TechUniversityofAgricultureandForestryYangling712100China

The shell traits and weight traits are measured in cultured populations of bay scallop, Argopecten irradians. The results of regression analysis show that the regression relationships for all the traits are significant (P0.05). The multiple regression equation is obtained to estimate live body weight and tissue weight. The above traits except anterior and posterior auricle length are used for the growth and production comparison among three cultured populations, Duncan's new multiple range procedure analysis shows that all the traits in the Lingshuiqiao (LSQ) population are much more significant than those of the other two populations (P0.05). The results indicate that the LSQ population has a higher growth rate and is expected to be more productive than the other two populations.

关键词： Argopecten irradians quantitative traits correlative analysis growth comparison

Studies on Pharmacokinetics of Porcine Somatotropin Liposomes

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Chemical Research in Chinese Universities 2005年第2期21卷 187-190页

作者： XUChuan-lai JICheng HAOKai LIXiang-qian YAOHui-yuan SchoolofFoodScienceandTechnology SouthernYangtzeUniversityWuxi214036P.R.China collegeofanimalscienceandtechnology ChinaAgriculturalUniversityBeijing100094P.R.China

This paper focuses on the pharmacokinetics in fattening pigs as well as the change of serum resulting from the use of porcine somatotropin(PST) and its liposomes. A slow-release model was determined to be relevant to following an examination of all dynamic parameters. The results indicate that the slow-release effect of PST liposomes was significant, with an extended release time of over 7 d.

关键词： Porcine somatotropin(PST) liposomes Pharamacokinetics Slow-release

Effect of Hydropqinone on Ruminal Urease in the Sheep and its Inhibition Kinetics in Vitro

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Journal of Northeast Agricultural University(English Edition) 2000年第2期7卷 101-108页

作者： ZHANGYong－g SHAN－An－shan collegeofanimalscienceandtechnology NortheastAgricultureUniversityHarbinHeilongjiang150030 CollegeofA

Effect of hydropuinone (HQ) on rumen urease acivity was studied. Hydroquinone at concentration of 0.01 mg·L -1 ,1 mg·L -1 and 10 mg·L -1 inhibited urease of intact rumen microbes in vitro by 25%, 34%, 55% and 64% respectively. In the present of low concentration of βmercaptoethanol,rumen urease could be solubilized and partially purified. The Km for the enzyme was 2×10 -3 mol·L -1 with Vmax of 319.144 μmoles/mg/*** kinetics of inhibition with partially purified rumen urease was *** result showed that the inhibuitory effect was not eliminated by increasing urea concentration indicating a noncompetitive in nature with inhibition constant 1.2×10 -5 mol·L -1 .Hydropuinone at a concentration that produced 64% urease inhibition did not affect ruminal total dehydrogenase, proteolytic enzyme( P >0.05)but increased cellulase activity by 28%( P <0.05)in *** results demonstrated that hydropuinone was a specific inhibutor of rumen urease and could delay urea hydrolysis effectively without negative *** inhibitor appeared to offer the potential to improve nitrogen utilization by ruminants fed diets containing urea.

关键词： Hydropuinone ruminal urease sheep kinetics

Effects of Ooplasmic Transfer on Rabbit Oocyte Fertilization and Early Embryonic Development

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Agricultural Sciences in China 2005年第7期4卷 542-549页

作者： LIJun-feng SONGYan-hua LIHai-feng WANGXian-zhong ZHANGJia-hua ChongqingKeyLabofForage＆Herbivore CollegeofAnimalScienceandTechnologySouthwestAgriculturalUniversityChongqing400716P.R.China

In order to evaluate the effects of ooplasm on oocyte fertilization and early embryonic development and to study themitochondrial DNA (mtDNA) heterogeneity of early embryos, microinjection was first performed to transfer a smallamount (5 to 7%) of donor ooplasm into recipient oocytes, then the eggs were fertilized with rabbit sperm throughintracytoplasmic sperm injection (ICSI). In group 1 (homogeneous ooplasmic transfer), both the donor and recipient rabbitoocytes were at metaphase II (MII). In group 2 (heterogeneous ooplasmic transfer), the donor was mouse MII oocyte andthe recipient was rabbit MII oocyte. In the control group, only ICSI was done on rabbit oocyte without ooplasmic *** significant difference (P>0.05) was observed in blastocyst development rates between group 1 (13.0%, 3/23) and thecontrol group (16.7%, 4/24), but significant difference (P<0.05) was examined in blastocyst development rate betweengroup 2 (0, 0/27) and the control group. Blastomeres cleaved unequally and embryonic fragments increased after ooplasmictransfer and ICSI. In early embryos, in group 2, donor mouse mtDNA was detected in 2-cell embryos (3/3), 4-cell embryos(3/4), 8-cell embryos (4/4), and morulae (2/2). The mtDNA fingerprinting analysis showed that mouse mtDNA detected inheterogeneous embryos of different developmental stages had exactly the same sequence as that of the donor mousemtDNA, thus indicating that homogenous ooplasmic transfer had no significant influence on rabbit oocyte fertilizationand early embryonic development, and that heterogeneous ooplasmic transfer did cause notable reduction in blastocystdevelopment rate. Heterogeneous mtDNA sequence in early embryos did not mutate. Compared with the control group,the embryonic quality declined after ooplasmic transfer operation in the present experiment.

关键词： Ooplasmic transfer ICSI Embryonic development mtDNA heterogeneity Fingerprinting

Histological Study on in vitro Co-cultivation of the Myocardium Tissue and Cells with Mouse Embryonic Fibroblasts

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Journal of Northeast Agricultural University(English Edition) 2004年第2期11卷 143-147页

作者： ZHANGGui-xue LIUYan HUPeng-fei collegeofanimalscienceandtechnology NortheastAgriculturalUniversityHarbinHeilongjiang150030PRC

The histological observation was experimentally conducted on in vitro cultured mouse embryonic myocardium cells and myocardiumoid cell mass. The mouse embryo tissue were cultured and regular pulsatile myocardiumoid tissue could be found. During in vitro culture, the myofilament bundles in the cell were gradually increasing and strongly connectted each other with embryonic age and there were loose muscle fibers initially and intercalated discs were close to each other. The lose myofilament bundles were developed in muscle fibers with age and the distance between intercalated discs was enlarged. There were myofilamentoid structure in inactive cells and filament peripherily.

关键词： mouse myocardium embryonic fibroblast histology in vitro co-culture

Study on Isolation, Passage, Cryopreservation and Histology of Mouse Fibroblasts

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Journal of Northeast Agricultural University(English Edition) 2004年第1期11卷 30-36页

作者： ZHANGGui-xue LIUYan HUPeng-fei collegeofanimalscienceandtechnology NortheastAgriculturalUniversityHarbinHeilongjiang150030PRC

The embryonic ages were determined for the best preparation of mouse fibroblasts. Four methods were adapted to verify cryopreservation of mouse fibroblasts. The results showed that embryonic cryopreserving method was best one with 0.86 of thawing viability. The embryos from 13-14 d pregnant mouse were superior to 11-12 d and 15-16 d in isolating, growing, laying and living. The first 6 generations were better than following ones in the same aspects above. Cell laying time became longer and vailable time became shorter after the sixth generation. With culture time increasing, fibroblast nuclear size became larger, fibrous filament appeared among fibroblasts, and macrocyst vesicle with floccule appeared in the cells. Cyst vesicle structure with pyknotic granule appeared in 24 h cultured fibroblasts and macrocyst vesicle also appeared in passaging fibroblasts.

关键词： fibroblast isolation passage cryopreservation histology

Oral Insulin Stimulates Intestinal Department and Enzyme Maturation in Newborn Pigs

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Agricultural Sciences in China 2004年第9期3卷 707-715页

作者： WANGTian XURuo-jun HUOYong-jiu collegeofanimalscienceandtechnology NanjingAgriculturalUniversityNanjing210095P.R.China DepartmentofZoology TheUniversityofHongKongP.R.China

The effects of oral insulin on intestinal tissue growth and brush border enzyme activitiesin newborn pigs were examined in this study. Newborn unsuckled pigs were bottle-fed for3 days with artificial milk(M), milk supplemented with 60mIUmL-1 of insulin(IM) orhydrolyzed milk(HM). Compared with newborn unsuckled pigs, piglets bottle-fed for 3 daysall gained in intestinal weight and length significantly despite a mild loss in bodyweight during the experimental period. The activities of lactase and alkaline phosphatase(AKP) in the small intestinal mucosa declined markedly in pigs fed with M, but theactivity of maltase increased significantly during the experimental period. Dietaryprotein pre-hydrolysis had no significant effect on intestinal tissue mass or length,but it moderated the decline of intestinal lactase and AKP activities. Dietarysupplementation of insulin significantly increased mucosal protein content and brushborder activities of lactase, maltase, AKP and aminopeptidase(AP)in the small *** effect of insulin treatment was particularly obvious at the distal region of the smallintestine. These results demonstrate that oral insulin can stimulate intestinal digestiveenzyme activities in newborn pigs. The finding supports the hypothesis that milk-borneinsulin plays a role in regulating postnatal gut development in the suckling young.

关键词： Insulin Intestine Enzyme Pig