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Journal of Environmental sciences 2019年第3期31卷 229-237页

作者： Wenjuan Han Guiying Luo Bin Luo Chenchen Yu Hai Wang Jie Chang Ying Ge college of life sciences Zhejiang University college of Chemistry and life sciences Zhejiang Normal University college of life sciences Shaoxing University

Wastewater with relatively high nitrogen concentrations is a major source of nitrous oxide(N_2O) and methane(CH_4) emissions and exerts multiple stresses on the *** have shown that plant diversity plays an important role in ecosystem ***, the effects of plant species diversity on CH_4 and N_2O emissions under high ammonium(NH_4^+-N) loading rates remain unclear. In this study, a microcosm experiment simulating vertical constructed wetlands supplied with high NH_4^+-N water levels was established. The treatments included four species richness levels(1, 2, 3, 4) and 15 species compositions. There was no significant relationship between species richness and N_2O emissions. However, N_2O emissions were significantly reduced by specific plant species composition. Notably, the communities with the presence of Rumex japonicus L. reduced N_2O emissions by 62% compared to communities without this species. This reduction in N_2O emissions may have been a result of decreased N concentrations and increased plant biomass. CH_4 emissions did not respond to plant species richness or species ***, plant species identity surpassed species richness in lowering N_2O emissions from constructed wetlands with high NH_4^+-N water. The results also suggest that communities with R. japonicus could achieve higher N removal and lower greenhouse gas emissions than other wetland species.

关键词： Plant species richness Species identity Methane Nitrous oxide Nitrogen removal Ecosystem functioning

Cholesterol metabolism: physiological versus pathological aspects in intracerebral hemorrhage

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Neural Regeneration Research 2025年第4期20卷 1015-1030页

作者： Ruoyu Huang Qiuyu Pang Lexin Zheng Jiaxi Lin Hanxi Li Lingbo Wan Tao Wang Department of Forensic Science School of Basic Medicine and Biological SciencesSuzhou Medicine College of Soochow UniversitySuzhouJiangsu ProvinceChina

Cholesterol is an important component of plasma membranes and participates in many basic life functions,such as the maintenance of cell membrane stability,the synthesis of steroid hormones,and *** plays a key role in the establishment and maintenance of the central nervous *** brain contains 20%of the whole body’s cholesterol,80%of which is located within myelin.A huge number of processes(e.g.,the sterol regulatory element-binding protein pathway and liver X receptor pathway)participate in the regulation of cholesterol metabolism in the brain via mechanisms that include cholesterol biosynthesis,intracellular transport,and *** brain injuries or diseases involving crosstalk among the processes above can affect normal cholesterol metabolism to induce detrimental ***,we hypothesized that cholesterol-related molecules and pathways can serve as therapeutic targets for central nervous system *** hemorrhage is the most severe hemorrhagic stroke subtype,with high mortality and *** cholesterol levels are associated with the risk of intracerebral ***,secondary pathological changes after intracerebral hemorrhage are associated with cholesterol metabolism dysregulation,such as neuroinflammation,demyelination,and multiple types of programmed cell *** cholesterol accumulation in the brain has been found after intracerebral *** this paper,we review normal cholesterol metabolism in the central nervous system,the mechanisms known to participate in the disturbance of cholesterol metabolism after intracerebral hemorrhage,and the links between cholesterol metabolism and cell *** also review several possible and constructive therapeutic targets identified based on cholesterol metabolism to provide cholesterol-based perspectives and a reference for those interested in the treatment of intracerebral hemorrhage.

关键词： cell death cholesterol metabolism intracerebral hemorrhage myelination therapeutic target

Identification of Reference Genes for Normalizing Quantitative Real-Time PCR in Urechis unicinctus

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Journal of Ocean University of China 2018年第3期17卷 614-622页

作者： BAI Yajiao ZHOU Di WEI Maokai XIE Yueyang GAO Beibei QIN Zhenkui ZHANG Zhifeng Key Laboratory of marine Genetics and Breeding of Ministry of Education College of Marine Life SciencesOcean University of China

The reverse transcription quantitative real-time PCR(RT-qPCR) has become one of the most important techniques of studying gene expression. A set of valid reference genes are essential for the accurate normalization of data. In this study, five candidate genes were analyzed with ge Norm, Norm Finder, Best Keeper and ?Ct methods to identify the genes stably expressed in echiuran Urechis unicinctus, an important commercial marine benthic worm, under abiotic(sulfide stress) and normal(adult tissues, embryos and larvae at different development stages) conditions. The comprehensive results indicated that the expression of TBP was the most stable at sulfide stress and in developmental process, while the expression of EF-1-α was the most stable at sulfide stress and in various tissues. TBP and EF-1-α were recommended as a suitable reference gene combination to accurately normalize the expression of target genes at sulfide stress; and EF-1-α, TBP and TUB were considered as a potential reference gene combination for normalizing the expression of target genes in different tissues. No suitable gene combination was obtained among these five candidate genes for normalizing the expression of target genes for developmental process of U. unicinctus. Our results provided a valuable support for quantifying gene expression using RT-qPCR in U. unicinctus.

关键词： reference gene RT-qPCR sulfide stress tissue developmental process Urechis unicinctus

Molecular Characterization, Expression Profiles, and Immunostimulation Responses of TRAF6 and TAK1 in Japanese Flounder(Paralichthys olivaceus)

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Journal of Ocean University of China 2019年第1期18卷 165-176页

作者： ZHAO Haitao CHEN Yan WANG Zhigang ZHANG Quanqi YU Haiyang college of marine life sciences Ocean University of China Key Laboratory of Marine Genetics and BreedingMinistry of Education

Tumor necrosis factor receptor-associated factor 6(TRAF6) and transforming growth factor-β-activated kinase 1(TAK1) are two important adaptor molecules in Toll-like receptor(TLR) signaling pathway. In this study, TRAF6(PoTRAF6) and TAK1(PoTAK1) were cloned and characterized in Japanese flounder(Paralichthys olivaceus). The full-length cDNA sequence of Po TRAF6 is 1953 bp, with an open reading frame(ORF) of 1713 bp encoding a putative protein of 570 amino acids. PoTRAF6 contains one really interesting new gene(RING) domain, two zinc fingers, one coiled-coil region, and one meprin and TRAF homology(MATH) domain, which shows a high similarity to TRAF6 s in other species. The full-length PoTAK1 cDNA sequence is 2086 bp, with an ORF of 1728 bp that encodes a putative protein of 575 amino acids. PoTAK1 contains a conserved serine/threonine protein kinase catalytic domain and a coiled-coil region. The promoter regions of PoTRAF6 and PoTAK1 were also analyzed to predict several potential transcription factor-binding sites. In addition, the expression patterns of these two genes were examined in developmental stages, different tissues, and challenged samples. PoTRAF6 and PoTAK1 were expressed during the whole developmental stages, and the highest expressions were in intestine and heart, respectively. In challenged embryonic cells with LPS, CpG ODN, and poly I:C, the expressions of PoTRAF6 and PoTAK1 were both up-regulated significantly. These results suggest that PoTRAF6 and Po TAK1 play crucial roles in immune responses and may be involved in the developmental process of Japanese flounder.

关键词： TRAF6 TAK1 Paralichthys olivaceus cloning expression promoter analysis immunostimulation

Variations of antioxidant enzyme activity and malondialdehyde content in nemertean Cephalothrix hongkongiensis after exposure to heavy metals

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Chinese Journal of Oceanology and Limnology 2010年第4期28卷 917-923页

作者：吴海一赵夕旦孙世春 marine Culture Research Laboratory Ocean University of China college of marine life sciences Ocean University of China

The antioxidant enzyme activity and malondialdehyde(MDA) content of Cephalothrix hongkongiensis were studied to assess variations in the biochemical/physiological parameters of nemerteans under heavy metal *** were exposed to copper,zinc and cadmium solutions at different concentrations,and the activity of three antioxidant enzymes,catalase(CAT),superoxide dismutase(SOD),and glutathione peroxidase(GPX),and MDA content were *** results show that the activity of each enzyme changed immediately after exposure to heavy *** was invariably inhibited throughout the experimental period,while the SOD activity was significantly elevated by exposure to Cu^(2+) for 48h,but then *** was inhibited by Zn^(2+) during the first 12h of exposure,but activated when exposed for longer *** Cd^(2+) stress,SOD activity decreased within *** activity varied greatly,being significantly increased by both Cu^(2+) and Zn^(2+),but significantly inhibited by Cd^(2+) in the first 12-24h after *** content increased on Cu^(2+) exposure,but normally decreased on Zn^(2+) *** content followed an increase-decrease-increase pattern under Cd^(2+) *** conclusion,the antioxidant system of this nemertean is sensitive to heavy metals,and its CAT activity may be a potential biomarker for monitoring heavy metal levels in the environment.

关键词： Cephalothrix hongkongiensis catalase superoxide dismutase glutathione peroxidase malondialdehyde heavy metal

Cytogenetic analysis in two scallops (Bivalvia:Pectinidae) by PRINS and PI banding

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Acta Oceanologica Sinica 2007年第5期26卷 153-158页

作者： ZHANG Lingling BAO Zhenmin WANG Jue WANG Shi HUANG Xiaoting HU Xiaoli HU Jingjie Laboratory of marine Genetics and Breeding College of Marine Life Sciences Ocean University of China Qingdao 266003 China

Cytogenetic analysis was performed for the bay scallop （Argopecten irradians Lamarck 1819） and the Japanese scallop （Patinopecten yessoensis Jay 1857 ） by primed in situ labeling （PRINS） and propidium iodide （PI） banding techniques. The PRINS analysis revealed that major rRNA genes were clustered in two loci on the telomeric regions of the short arms on two acrocentric chromosome pairs in A. irradians and on two submetacentric pairs in P. yessoensis. The histone H3 gene sites differed in number and location between these two species. The C-band-like patterns revealed by PI staining varied considerably between these two species. A. irradians displayed terminal bands at long arms on all chromosomes, centromeric bands on some pairs and interstitial bands on five pairs. P. yessoensis exhibited only centromeric bands on all chromosomes. These results would contribute to the better understanding of karyotype evolution in A. irradians and P. yessoensis.

关键词： primed in situ labeling PI banding Argopecten irradians Patinopecten yessoensis

MiR-430 Can Affect the Mesoderm Formation and Metamorphosis of Paralichthys olivaceus by Targeting lefty Gene

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Journal of Ocean University of China 2020年第2期19卷 409-416页

作者： LI Rui WANG Zhenwei SUN Minmin PENG Meiting QI Jie WANG Zhigang Key Laboratory of marine Genetics and Breeding Ministry of Education College of Marine Life SciencesOcean University of China

MiR-430 is a non-coding small RNA that participates in early embryonic development by acting on the Nodal signaling *** functions in Paralichthys olivaceus(***),however,remain *** this study,through biological information technology,we hypothesized lefty was the target gene of *** is a divergent member of the transforming growth factor-β(TGF-β)superfamily,and is involved in regulating left-right patterning as an antagonist of the Nodal signaling *** study the relationship between miR-430 and lefty gene,miR-430 and fluorescent expression vector were transfected into embryo *** results showed that miR-430 has important effects on early embryonic *** can inhibit the expressions of lefty and other genes related to Nodal signaling ***,the expression of miR-430 was involved in the metamorphosis of *** study clarifies the function of miR-430 in ***,which will be helpful for further research on the interactions between miR-430 and the Nodal signaling pathway.

关键词： miR-430 lefty Nodal signal pathway Paralichthys olivaceus

Development and utility of EST-SSR markers in Ulva prolifera of the South Yellow Sea

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Acta Oceanologica Sinica 2014年第10期33卷 105-113页

作者： ZHANG Lei WANG Guoliang LIU Cui CHI Shan LIU Tao college of marine life sciences Ocean University of China Beijing Institute of Genomics Chinese Academy of Sciences

Ulva species can grow rapidly in nutrient-rich habitats causing green tides and marine fouling. A more complete understanding of the reasons behind these outbreaks is urgently required. Accordingly, this study attempts to use microsatellite markers based expressed sequence tag（EST） to analyze the genetic variation of several Ulva prolifera populations in the South Yellow Sea of China. Two hundred and thirty-eight SSRs were identified from 8 179 unique ESTs（6 203 newly sequenced and 1 976 downloaded from NCBI database） and 37 primer pairs were successfully designed according to the ESTs; 11 pairs were selected to detect the genetic diversity and relationship of 69 attached U. prolifera samples and 13 free-floating samples collected from coastal and off-coast areas of the South Yellow Sea. The results of cross-species transferability showed that six of the 11 EST-SSR primers could give good amplification in other five Ulva species and the average allele number was 4.67. Genetic variation analysis indicated that all 82 U. prolifera samples were clearly divided and most samples collected from the same site clustered together as a group in the dendrogram tree produced by unweighted pair-group mean analysis（UPGMA） method and the cluster results showed some consistency with the geographical origins. In addition, 13 free-floating samples（except HT-001-2） were grouped as a single clade separated from the attached samples.

关键词： Ulva prolifera expressed sequence tag(EST) microsatellite genetic diversity seaweeds simple sequence repeat(SSR)

Genetic diversity and specific markers in four scallop species, Patinopecten yessoensis, Argopecten irradians, Chlamys nobilis and ***

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Acta Oceanologica Sinica 2005年第4期24卷 107-113页

作者： CHEN Shengping BAO Zhenmin PAN Jie HU Jingjie college of marine life sciences Ocean University of China Qingdao 266003 China college of life sciences Sun Yat-sen University Guangzhou 510275 China

The AFLP （amplified fragrnent length polymorphism）technique was used to analyse the genetic diversity in four scallop species, Patinopecten yessoensis, A rgopecten irradians, Chlamys nobilis and C. farreri. The genetic similarity indexes of these four species are 0.8415, 0.7863, 0.7190 and 0.6731, while Shannon diversity indexes are 43.52, 58.87, 80.16 and 92.83, respectively. As analyzed, the genetic diversities in two native species, i.e., C, farreri and C. nobilis, are higher than those in other two introduced species, A. irradians and P. yessoensis. The results also showed that C. nobilis and C, farreri shared the most common loci. The genetic distance indicated that C. nobilis and C. farreri are closely related. Moreover, out of 510 AFLP markers, 21 specific bands are found to distinguish the four species scallops and these markers may be applied to the specific germplasm characterization and molecular assistant classification in scallops.

关键词： scallops AFLP markers genetic diversity

Studies　on　morphocytology　of　Planktoniella　and　correction　of　nomenclature　of　Valdiviella　formosa　(Sc

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Acta Oceanologica Sinica 1997年第2期16卷 249-258页

作者： Qian, Shuben Wang, Wei college of marine life sciences Ocean University of Qingdao Qingdao 266003 China college of marine life sciences Ocean University of Qingdao China

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关键词： morphocytology and Studies Planktoniella correction