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Characterization of two novel heat shock protein 70s and their transcriptional expression patterns in response to thermal stress in adult of Frankliniella occidentalis (Thysanoptera:Thripidae)

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Journal of Integrative Agriculture 2018年第5期17卷 1023-1031页

作者： QIN Jing GAO Peng ZHANG Xiao-xiang LU Ming-xing DU Yu-zhou School of Horticulture and plant protection & institute of applied entomology Yangzhou University

Heat shock protein 70（HSP70） is one of the most important members in the heat shock protein family, and plays important roles in the thermotolerance of insect. To explore the molecular mechanism of thermotolerance of Frankliniella occidentalis adults, the difference in the expression of HSP70s in F. occidentalis male or female adults under the thermal stress was studied under the laboratory conditions. Two full length c DNAs of HSP70s gene（Fohsc704 and Fohsc705） were cloned from F. occidentalis by using RT-PCR and RACE. The genomic sequence was demonstrated by genomic validation, and the position and size of the intron were analyzed by sequence analysis of c DNA. Real-time PCR was used to analyze the HSP70 expression patterns. The c DNA of Fohsc704 and Fohsc705 possessed 2 073 and 1 476 bp which encoded 690 and 491 amino acids（aa） with a calculated molecular weight of 75 and 54 k Da, respectively. Four introns in Fohsc704 and six introns in Fohsc705 protein were found. However, the HSP70 protein sequences in our study were ended with EKKN and GIFL, which were different from the reported Fo HSP70s. Various expression patterns of Fohsc704 and Fohsc705 were found in both genders of F. occidentalis under thermal stress. The expression of Fohsc704 and Fohsc705 reached to the highest level at –12 and –8°C in male adults, respectively, and Fohsc705 expressed the highest level at 33°C in female adults. In conclusion, HSP70s of F. occidentalis in our study are novel heat shock proteins. There were difference in expression patterns of the two hsc70s in genders of F. occidentalis, and the two HSP70s play important roles in the thermotolerance of F. occidentalis.

关键词： Frankliniella occidentalis HSP70 temperature gene cloning expression

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Minerals in edible insects:a review of content and potential for sustainable sourcing

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Food Science and Human Wellness 2024年第1期13卷 65-74页

作者： Mingxing Lu Chenxu Zhu Sergiy Smetana Ming Zhao Haibo Zhang Fang Zhang Yuzhou Du college of plant protection&institute of applied entomology Yangzhou UniversityYangzhou 225009China German institute of Food Technologies(DIL e.V.) Quakenbrück D-49610Germany Jiangsu plant protection and plant Quarantine Station Nanjing 210036China Joint International Research Laboratory of Agriculture and Agri-Product Safety Yangzhou UniversityYangzhou 225009China

In response to the rapid increase in world population and subsequent demands for food,edible insects represent an alternative food source for humans that is rich in proteins,amino acids and *** is a tradition in many countries including China and Thailand,and edible insects have attracted a lot of attention in Western World due to their suitable nutrient composition,high mineral content(e.g.,Fe,Zn,Ca,Mg)and potential use as a supplement in human *** this study,we surveyed mineral content in seven insect orders and 67 species of mass produced and wild-harvested edible *** total content of essential elements in edible insects was very high in Tenebrio molitor,Bombyx mori,and Zonocerus *** heavy metal content(summarized for eight species)was below the maximum limit allowed for safe *** supply of minerals derived from insect biomass is complicated due to the high variations of mineral content in insects and the potential of its change due to processing.

关键词： Edible insects Minerals Nutrition Food safety Sustainability

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Four new feather mite species of the genus Proctophyllodes Robin(Astigmata:Proctophyllodidae) from China

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动物分类学报 2014年第2期39卷 248-258页

作者： Zi-Ying Wang Jin-Jun Wang Xiao-Hui Su institute of entomology College of Plant ProtectionSouthwest University

Four new species of the feather mite genus Proctophyllodes are described from the birds of the order Passeriformes in China： P. flexuosa sp. nov. from Eophona migratoria Hartert （Chongqing）, P. brevis sp. nov. from Passer montanus （Linnaeus）（Anhui and Chongqing）, P. garrula sp. nov. from Garrulaxpoecilorhynchus Gould （Anhui）, P. canora sp. nov. from Garrulax canorus （Linnaeus）（Guizhou）.

关键词： Astigmata feather mites Proctophyllodes new species China.

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Comparative morphological study on the genus Leptura Linnaeus of China(Coleoptera: Cerambycidae: Lepturinae)

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Zoological Systematics 2014年第3期39卷 395-410页

作者： Jie-Qun Ren Gui-Qiang Huang Si-Yao He Li Chen Zhi-Ping Liu college of plant protection Southwest University institute of entomology

A comparative morphological research was done based on the morphological characters of 21 species from Leptura in this paper. The results indicated that 18 morphological characters, such as the ratio of the lower eye lobe to genae, the relative length of antennae segments, the shape of maxillary palpi apex, prothorax, elytra apex and elytral maculae can be used to identify species in the genus. Meanwhile, the color of elytra and the size of elytral maculae could not be used to identify species from Leptura. In addition, the shape of 7th abdominally sternal apex, the length of antennae and the ratio of the first hind tarsal segment to the second can be used to distinguish the male and the female.

关键词： Comparative morphology Cerambycidae Leptura China.

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Analysis of a late gene, orfl01 from Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus

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Insect Science 2005年第5期12卷 335-340页

作者： SHI-HENG AN LI-PING XING V. SHYAM KUMAR CHUAN-XI ZHANG institute of applied entomology Zhejiang University Hangzhou college of plant protection Henan Agricultural University Zhengzhou China

Helicoverpa armigera single nucleocapsid nucleopolyhedrovirns open reading frame 101 （ha101） is 762 nts in length and encodes a 254 amino acid peptide with predicted 29 kDa molecular weight. The homologues of ha101 were explored using BLASTP searching tool in the updated GenBank/EMBL and SWlSS-PROT databases. The results showed that the homologues of ha101 were present in all the completely sequenced lepidopteran nucleopolyhedrovirnses and granulovirnses, suggesting that ha101 might be a functional gene associated with their lepidopteran hosts. Sequence alignment of ha101 and its homologues revealed that 10 amino acids were completely conserved. RT-PCR analysis of ha101 manifested that the transcript of ha101 was first detected at 24 hpi and remained detectable at up to 122 hpi, suggesting that ha101 was transcribed during late stages of infection. Ha101 was expressed using Bac to Bac system in Tn5B-1-4 cells. The product of ha101 expressed in Tn5B-1-4 cells was approximately 29kDa, consistent with the predicted molecular weight, and the results were confirmed by western blot analysis. The subcellular localization indicated that ha101 was aggregated along nuclear envelope during the early stages of infection and spread out to the entire nucleus including virogenic stroma in late stages of infection, suggesting that ha101 may play a specific role in virion assembly process or virogenic stroma arrangement.

关键词： expression ha101 Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus sequence analysis RT-PCR subcellular localization

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Increased nitrogen fertilization inhibits the biocontrol activity promoted by the intercropping partner plant

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Insect Science 2021年第4期28卷 1179-1190页

作者： Leyun Wang Hongying Cui Xinyue Chang Mengmeng Zhu Zihua Zhao Department of entomology College of Plant ProtectionChina Agricultural UniversityBeijingChina institute of plant protection Ningxia Academy of Agricultural and Forestry SciencesYinchuanChina

The examination of the compatibility between agricultural practices and biocontrol activities is crucial for establishing an efficient,eco-friendly,and sustainable pest management *** this study,we examined the population dynamics of two specialist aphids,the English grain aphid(Sitobion avenae)on potted wheat and the pea aphid(Acyrthosiphon pisum)on potted alfalfa,as well as the biocontrol activity of a generalist predator,the harlequin ladybird beetle(Harmonia axyridis).We investigated their responses to the presence of the intercropping partner plant species(alfalfa and wheat,respectively)through plant volatiles or visual cues at three nitrogen fertilizer levels in a *** the absence of the predator,the English grain aphid population growth rate increased significantly with increasing nitrogen levels,whereas the pea aphid population increased significantly more slowly in response to high nitrogen *** English grain aphid and pea aphid population dynamics were unaffected by the presence of the intercropping ***,the presence of the intercropping partner enhanced the control of both aphid populations by the harlequin ladybird *** nitrogen fertilizer levels decreased the predation rates,which were otherwise increased by the intercropping *** beneficial effects of the intercropping partner were eventually non-existent at the highest nitrogen level *** results imply that the interaction between the presence of intercropping partner and the nitrogen fertilizer application affects the biocontrol activity of the natural enemies of insect ***,the compatibility between agricultural intensification and biocontrol strategies in integrated pest management programs need to be investigated.

关键词： Acyrthosiphon pisum biological control habitat Harmonia axyridis nitrogen fertilizer Sitobion avenae

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Two new record species or subspecies of the genus Leptura Linnaeus from China (Coleoptera: Cerambycidae: Lepturinae)

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Zoological Systematics 2014年第4期39卷 588-590页

作者： Jie-Qun Ren Li Chen college of plant protection Southwest UniversityInstitute of Entomology

Two species, Leptura subtilis Bates, 1884 and L. ochraceofasciata ochrotela Bates, 1873 are firstly recorded from China. Morphological descriptions, distributions and morphological figures are presented. Specimens exa... 详细信息

关键词： Coleoptera Cerambycidae new record China

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Characterization of T-complex polypeptide 1 (TCP-1) from the Chilo suppressalis HSP60 family and its expression in response to temperature stress

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Journal of Integrative Agriculture 2018年第5期17卷 1032-1039页

作者： YU Tong-ying LU Ming-xing CUI Ya-dong Biology and Food Engineering School Fuyang Normal University School of Horticulture and plant protection & institute of applied entomology Yangzhou University

Many proteins require assistance from molecular chaperones at various stages to attain correctly folded states and functional conformations during protein synthesis. In this study, the gene encoding T-complex polypeptide 1（TCP-1）, which belongs to the heat shock protein 60（HSP60） family, was isolated and characterized from the rice stem borer, Chilo suppressalis, by RACE and q PCR, respectively. The full-length c DNA of Tcp-1 was 2 144 bp and encoded a 1 635-bp ORF; the deduced translational product contained 545 amino acids with 5′-and 3′-UTRs and an isoelectric point of 5.29. Cluster analysis confirmed that the deduced amino acid sequence shared high identity（60–99%） with TCP-1 from other insects. To investigate Tcp-1 expression in response to abiotic stress, q PCR was used to analyze expression levels of Tcp-1 m RNA in C. suppressalis larvae exposed to temperatures ranging from –11 to 43°C. With respect to heat shock, Tcp-1 expression was higher than the control after a 2-h exposure to 30 and 36°C and declined at 39 and 43°C. Difference in Tcp-1 expression was observed at temperatures ranging from –11 to 27°C. q PCR analyses revealed that Tcp-1 expression was the highest in hindgut tissue as compared to heads, epidermis, fat body, foregut, midgut, and malpighian tubules. Our results indicated that Tcp-1 expression was differentially expressed in C. suppressalis tissues, and was impacted by temperature stress.

关键词： TCP-1 Chilo suppressalis expression analysis temperature stress insect tissues

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A microbiological assay for biotin determination in insects

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Insect Science 2021年第2期28卷 415-418页

作者： Fei-Rong Ren Bing Bai Ji-Sheng Hong Yan-Zhen Huang Jun-Bo Luan Liaoning Key Laboratory of Economic and applied entomology College of Plant ProtectionShenyang Agricultural UniversityShenyangChina college of plant protection Shenyang Agricultural UniversityShenyangChina

Dear Editor,As the coenzyme of multiple carboxylases,biotin plays key roles in intermediary metabolism and animal nutrition(Listed 1990;Douglas,2017).Therefore,the analytical determination of biotin levels in diverse materials is a critical aspect of B-vitamin *** assays are classic methods of measuring B-vitamin ***,the growth of B-vitamin-dependent microorganisms is proportional to the amount of the specific B-vitamin in the medium,and the growth can be measured by assessing the change in the medium's turbidity.

关键词： determination vitamin critical

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The temporal-spatial expression and functional analysis of three gustatory receptor genes in Solenopsis invicta using sweet and bitter compounds

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Insect Science 2024年第2期31卷 448-468页

作者： Muhammad Irfan Waris Yanyuan Lei Guojun Qi Ziying Guan Abdul Rashied Jie Chen Lihua Lyu institute of plant protection Guangdong Academy of Agricultural SciencesGuangdong Provincial Key Laboratory of High Technology for Plant ProtectionGuangzhouChina Department of entomology College of Plant ProtectionSouth China Agricultural UniversityGuangzhouChina

The insect gustatory system participates in identifying potential food sources and avoiding toxic *** this process,gustatory receptors(GRs)recognize feeding stimulant and deterrent ***,the GRs involved in recognizing stimulant and deterrent compounds in the red imported fire ant,Solenopsis invicta,remain ***,we conducted a study on the genes SinvGR1,SinvGR32b,and SinvGR28a to investigate the roles of GRs in detecting feeding stimulant and deterrent *** this current study,we found that sucrose and fructose are feeding stimulants and the bitter compound quinine is a feeding *** fire ant workers showed significant behavior changes to avoid the bitter taste in feeding stimulant *** transcription quantitative real-time polymerase chain reaction results from developmental stages showed that the SinvGR1,SinvGR32b,and SinvGR28a genes were highly expressed in fire ant ***-specific expression profiles indicated that SinvGR1,SinvGR32b,and SinvGR28a were specifically expressed in the antennae and foreleg tarsi of workers,whereas SinvGR32b gene transcripts were also highly accumulated in the male ***,the silencing of SinvGRI or SinvGR32b alone and the co-silencing of both genes disrupted worker stimulation and feeding on sucrose and *** results also showed that SinvGR28a is required for avoiding quinine,as workers with knockdown of the SinvGR28a gene failed to avoid and fed on *** study first identified stimulant and deterrent compounds of fire ant workers and then the GRs involved in the taste recognition of these *** study could provide potential target gustatory genes for the control of the fire ant.

关键词： behavioral responses feeding assays gustatory receptors RNA interference Solenopsis invicta temporal-spatial expression

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