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Acta pharmaceutica Sinica B 2024年第8期14卷 3783-3784页

作者： Wan Li Liwen Ren Xiangjin Zheng Jinyi Liu Jinhua Wang Tengfei Ji Guanhua Du The State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia MedicaChinese Academy of Medical Science and Peking Union Medical CollegeBeijing 100050China Laboratory of Drug Target Research and Drug Screen Institute of Materia MedicaChinese Academy of Medical Science and Peking Union Medical CollegeBeijing 100050China

The authors apologize for two inadvertent mistakes in Fig.5:the image of immunofluorescence of LC3B in the Vehicle group(Fig.5c)and the image of transmission electron microscope in the AKBA 10 mmol/L group(Fig.5f)were provided *** corrected Fig.5 is shown *** original data of these figures have been provided to the Editorial Office,and the corresponding authors or the Editorial Office can be contacted for original data access.

关键词： correction Office image

Molecular Pathway of Psoralidin-Induced Apoptosis in HepG2 Cell Line

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Chinese Journal of Integrative Medicine 2019年第10期25卷 757-762页

作者： YU Bin WANG An-hong ZHOU Kun CHAI Li-juan LIU Lu School of Integrative Medicine Tianjin University of Traditional Chinese Medicine Tianjin (300193) China Institute of Traditional Chinese Medicine Tianjin University of Traditional Chinese Medicine Tianjin (300193) China Department of pharmacy Gansu Provincial Hospital Lanzhou (731600) China Tianjin State Key Laboratory of Modern Chinese Medicine Tianjin (300193) China college of pharm acy Henan University Kaifeng Henan Province (475004) China

Objective: To test the role of psoralidin in human liver cancer HepG2 cells in vitro. Methods: Cell viability was assessed by methylthiazolyldiphenyl-tetrazolum bromide assay and apoptotic cells were labeled by annexin V then sorted by flow cytometry. Protein expressions of caspase-3, caspase-8, caspase-9, Bax, Bid, Bcl-2, Bcl-xL and p53 were examined by western blot while activity of caspase-3,-8 and -9 were also determined. Results: Psoralidin reduces cell viability greatly in a time dependent manner(64%, 40%, 21%, 12% at 2, 6, 24 and 48 h treatment with 64 μmol/L psoralidin respectively) and up-regulates activities of caspase-3,-8 and-9 in a concentration dependent manner(between 4 to 64 μmol/L). Psoralidin also increases the expression of pro-apoptosis genes Bax, Bid and p53 while decreases the expression of pro-survival genes Bcl-2 and Bcl-xL, both in a concentration dependent manner between 4 and 64 μmol/L(P<0.05 at 16 and 64 μmol/L). Caspase-3 inhibitor(Ac-DEVD-CHO at concentrations between 10 to 20 μmol/L), p53 inhibitor(pifithrin-α at 5 μmol/L) and cyclosporin A can attenuate the apoptotic effect of psoralidin. Conclusion: The cytotoxic role of psoralidin might work through both intrinsic and extrinsic apoptotic pathway.

关键词： psoralidin apoptosis HepG2 mitochondria p53 cyclosporin A

Mechanism of pachymic acid in the treatment of gastric cancer based on network pharmacology and experimental verification

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World Journal of Gastrointestinal Oncology 2024年第1期16卷 30-50页

作者： Yu-Hua Du Jian-Jun Zhao Xia Li Shi-Cong Huang Na Ning Guo-Qing Chen Yi Yang Yi Nan Ling Yuan college of pharmacy Ningxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina Ningxia Chinese Medicine Research Center Manufacturing LaboratoryYinchuan 750004Ningxia Hui Autonomous RegionChina college of Foundation Ningxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina Key Laboratory of Ningxia Minority Medicine Modernization Ministry of Education Ningxia Medical UniversityYinchuan 750004Ningxia Hui Autonomous RegionChina

BACKGROUND Pachymic acid(PA)is derived from Poria *** has a variety of pharmacological and inhibitory effects on various ***,the mechanism of action of PA in gastric cancer(GC)remains *** To investigate the mechanism of PA in treating GC via the combination of network pharmacology and experimental *** The GeneCards and OMIM databases were used to derive the GC targets,while the pharm Mapper database provided the PA *** the STRING database,a protein-protein interaction network was constructed and core targets were *** analyses of Gene Ontology,Kyoto Encyclopedia of Genes and Genomes(KEGG),and gene set enrichment analysis were conducted,and molecular docking and clinical correlation analyses were performed on the core ***,the network pharmacology findings were validated through in vitro cell assays,encompassing assessments of cell viability,apoptosis,cell cycle,cloning,and western blot *** According to network pharmacology analysis,the core targets were screened,and the PI3K/AKT signaling pathway is likely to be the mechanism by which PA effectively treats GC,according to KEGG enrichment *** experimental findings showed that PA could control PI3K/AKT signaling to prevent GC cell proliferation,induce apoptosis,and pause the cell *** Network pharmacology demonstrated that PA could treat GC by controlling a variety of signaling pathways and acting on a variety of *** has also been supported by in vitro cell studies,which serve as benchmarks for further research.

关键词： Pachymic acid Gastric cancer Network pharmacology Enrichment analysis Cell proliferation

Effects of the Syzygium aromaticum L. extract on antioxidation and inhibition of matrix metalloproteinase in human dermal fibroblast

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Asian Pacific Journal of Tropical Biomedicine 2019年第2期9卷 53-59页

作者： Da Eun Kim Yeon Sil Hwang Bo Yoon Chang Dae Sung Kim Hyoung Kwon Cho Sung Yeon Kim Institute of pharmaceutical Research and Development College of PharmacyWonkwang University Hanpoong pharm.CO. Ltd

Objective: To investigate cosmetic potential of Syzygium aromaticum L.(S. aromaticum L.) and to determine its antioxidant and anti-wrinkling effects. Methods: Using highperformance liquid chromatography, eugenol component was quantitated. The antioxidant activity of S. aromaticum L. was analyzed by 2,2-diphenyl-1-picrylhydrazyl radical scavenging and superoxide dismutase like activities. To determine cell viability, elastase and matrix metalloproteinase-1(MMP-1) activity, human dermal fibroblasts(HS68) were treated with S. aromaticum L. The inhibitory effect of S. aromaticum L. on tumor necrosis factor alpha induced MMPs expression in HS68 was analyzed by realtime-PCR. Results: The eugenol content was confirmed in S. aromaticum L. S. aromaticum L. was observed to have high 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and superoxide dismutase like activity. S. aromaticum L. had no cytotoxicity against the HS68 and dose-dependently increased elastase inhibition. Moreover, S. aromaticum L. significantly decreased MMP-1 content and inhibited gene levels of MMP-1, MMP-2, MMP-3 and MMP-9. Conclusions: The findings suggest that S. aromaticum L. has great potential as a cosmeceutical ingredient with antioxidant and antiwrinkling effects.

关键词： Syzygium aromaticum L Antioxidant Anti-aging Elastase Matrix metalloproteinase-1

Shuxuening Injection Inhibits Apoptosis and Reduces Myocardial Ischemia-Reperfusion Injury in Rats through PI3K/AKT Pathway

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Chinese Journal of Integrative Medicine 2024年第5期30卷 421-432页

作者： YUE Tong-tong CAO Ying-jie CAO Ya-xuan LI Wei-xia WANG Xiao-yan SI Chun-ying XIA Han ZHU Ming-jun TANG Jin-fa WANG He The First Clinical Medical college of Henan University of Chinese Medicine Zhengzhou 450046China Department of pharmacy the First Affiliated Hospital of Henan University of Chinese MedicineZhengzhou 451200China Department of Cardiovascular Diseases the First Affiliated Hospital of Henan University of Chinese MedicineZhengzhou 451200China

Objective: To investigate the main components and potential mechanism of Shuxuening Injection(SXNI) in the treatment of myocardial ischemia-reperfusion injury(MIRI) through network pharmacology and in vivo research. Methods: The Traditional Chinese Medicine Systems pharmacology(TCMSP) and pharm Mapper databases were used to extract and evaluate the effective components of Ginkgo biloba leaves, the main component of SXNI. The Online Mendelian Inheritance in Man(OMIM) and Gene Cards databases were searched for disease targets and obtain the drug target and disease target intersections. The active ingredient-target network was built using Cytoscape 3.9.1 software. The STRING database, Metascape online platform, and R language were used to obtain the key targets and signaling pathways of the anti-MIRI effects of SXNI. In order to verify the therapeutic effect of different concentrations of SXNI on MIRI in rats, 60 rats were first divided into 5 groups according to random number table method: the sham operation group, the model group, SXNI low-dose(3.68 mg/kg), medium-dose(7.35 mg/kg), and high-dose(14.7 mg/kg) groups, with 12 rats in each group. Then, another 60 rats were randomly divided into 5 groups: the sham operation group, the model group, SXNI group(14.7 mg/kg), SXNI+LY294002 group,and LY294002 group, with 12 rats in each group. The drug was then administered intraperitoneally at body weight for 14 days. The main biological processes were validated using in vivo testing. Evans blue/triphenyltetrazolium chloride(TTC) double staining, hematoxylin-eosin(HE) staining, terminal deoxynucleotidyl transferase d UTP nick end labeling(TUNEL) assay, enzyme-linked immunosorbent assay(ELISA), and Western blot analysis were used to investigate the efficacy and mechanism of SXNI in MIRI rats. Results: Eleven core targets and 30 Kyoto Encyclopedia of Genes and Genomes(KEGG) pathways were selected. Among these, the phosphoinositide 3-kinase(PI3K)/protein kinase B(AKT) pathway was

关键词： myocardial ischemia reperfusion injury PI3K/AKT signaling pathway Shuxuening Injection apoptosis Ginkgo biloba leaves

Corrigendum to“Targeted metabolomics reveals the aberrant energy status in diabetic peripheral neuropathy and the neuroprotective mechanism of traditional Chinese medicine JinMaiTong”[***.14(2024)225e243]

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Journal of pharmaceutical Analysis 2024年第4期14卷 622-622页

作者： Bingjia Zhao Qian Zhang Yiqian He Weifang Cao Wei Song Xiaochun Liang Department of Traditional Chinese Medicine Peking Union Medical College HospitalPeking Union Medical CollegeChinese Academy of Medical SciencesBeijing100730China Institute of Basic Medical Sciences Chinese Academy of Medical SciencesBeijing100005China Medical Research Center Peking Union Medical College HospitalPeking Union Medical CollegeChinese Academy of Medical SciencesBeijing100730China

Original statement in the caption of Fig.5:***(JMT)treatment restored the perturbed central carbohydrate metabolism in the circulation and sciatic nerve of diabetic peripheral neuropathy(DPN)*** line arrows indicate the direct metabolic reactions,and solid line arrows indicate the indirect reactions.*P<0.05 and**P<***:no significant ***:control;TCA:tricarboxylic acid cycle;ADP:adenosine diphosphate:ATP:adenosine triphosphate;GDP:guanosine diphosphate;GTP:guanosine triphosphate.

关键词： protective neuropathy metabolism

3D interlocked all-textile structured triboelectric pressure sensor for accurately measuring epidermal pulse waves in amphibious environments

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Nano Research 2024年第3期17卷 1923-1932页

作者： Shaobo Si Chenchen Sun Yufen Wu Jingjing Li Han Wang Yinggang Lin Jin Yang Zhong Lin Wang Department of Optoelectronic Engineering Key Laboratory of Optoelectronic Technology and Systems Ministry of EducationChongqing UniversityChongqing 400044China college of Physics and Electronic Engineering Chongqing Normal UniversityChongqing 401331China Beijing Institute of Nanoenergy and Nanosystems Chinese Academy of SciencesBeijing 100083China School of Materials Science and Engineering Georgia Institute of TechnologyAtlantaGA 30332USA

The performance degradation and even damage of the e-textiles caused by sweat,water,or submersion during all-weather health monitoring are the main reasons that e-textiles have not been commercialized and routinized so ***,we developed an amphibious,high-performance,air-permeable,and comfortable all-textile triboelectric sensor for continuous and precise measurement of epidermal pulse waves during full-day *** on the principle of preparing gas by acid-base neutralization reaction,a one-piece preparation process of amphibious conductive yarn(acy)with densely porous structures is *** innovative three-dimensional(3D)interlocking fabric knitted from acys(0.6 mm in diameter)and polytetrafluoroethylene yarns exhibit high sensitivity(0.433 V·kPa^(-1)),wide bandwidth(up to 10 Hz),and stability(>30,000 cycles).With these benefits,98.8%agreement was achieved between wrist pulse waves acquired by the sensor and a high-precision laser ***,the polytetrafluoroethylene yarn with good compression resilience provides sufficient mechanical support for the contact separation of the ***,the unique skeletonized design of the 3D interlocking structure can effectively relieve the water pressure on the sensor surface to obtain stable and accurate pulse waves(underwater depth of 5 cm).This achievement represents an important step in improving the practicality of e-textiles and early diagnosis of cardiovascular diseases.

关键词： triboelectric pressure sensor amphibious fabric interlocking fabric pulse wave measurement one-piece preparation method

Increase in neurokinin-1 receptor-mediated colonic motor response in a rat model of irritable bowel syndrome

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World Journal of Gastroenterology 2005年第2期11卷 237-241页

作者： Jun-Ho La Tae-Wan Kim Tae-Sik Sung Hyn-Ju Kim Jeom-Yong Kim Il-Suk Yang Department of Physiology College of Veterinary MedicineSeoul National UniversityRepublic of Korea Department of Physiology College of Veterinary MedicineKyungpuk National UniversityRepublic of Korea Institute of Bioscience and Biotechnology Daewoong Pharm Co.Ltd.Republic of Korea

AIM: Irritable bowel syndrome (IBS) is a functional bowel disorder. Its major symptom is bowel dysmotility, yet the mechanism of the symptom is poorly understood. Since the neurokinin-1 receptor (NK1R)-mediated signaling in the gut is important in the control of normal bowel motor function,we aimed to investigate whether the NK1R-mediated bowel motor function was altered in IBS, using a rat IBS model that was previously reported to show colonic dysmotility in response to restraint ***: IBS symptoms were produced in male SpragueDawley rats by inducing colitis with acetic acid. Rats were left to recover from colitis for 6 d, and used for experiments 7 d post-induction of colitis. Motor activities of distal colon were recorded in ***: The contractile sensitivity of isolated colon to a NK1R agonist [Sar9, Met(O2)11]-substance P (1-30 nmol/L)was higher in IBS rats than that in normal rats. After the enteric neurotransmission was blocked by tetrodotoxin (TTX, 1 μmol/L), the contractile sensitivity to the NK1R agonist was increased in normal colon but not in IBS rat colon. The NK1R agonist-induced contraction was not different between the two groups when the agonist was challenged to the TTX-treated colon or the isolated colonic myocytes. A nitric oxide synthase inhibitor Nω-nitro-L-arginine methyl ester (L-NAME, 100 μmol/L) augmented the NK1R agonist-induced contraction only in normal rat ***: These results suggest that the NK1R-meidated colonic motor response is increased in IBS rats, due to the decrease in the nitrergic inhibitory neural component.

关键词： Irritable bowel syndrome Neurokinin-1 receptor

Development of an eco-friendly and fast HPLC method for quantitative analysis of four nucleosides in Cordyceps and related products

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Chinese Journal of Natural Medicines 2021年第12期19卷 954-960页

作者： QIAN Zheng-Ming WU Zi HUANG Qi WANG Chuan-Xi TAN Guo-Yin LI Wen-Jia SUN Wan-Yang LV Gen-Pin GAO Hao Institute of Traditional Chinese Medicine and Natural Products College of PharmacyJinan UniversityGuangzhou 510632China Ruyuan HEC pharm Co. Ltd.Shaoguan 512700China

An eco-friendly and fast HPLC method was developed for the determination of adenosine, inosine, guanosine and uridine in Cordyceps and related products(fermented mycelia of Hirsutella sinensis and Paecilomyces hepiali). The sample was ultrasonically extracted using 0.5% phosphoric acid solutions for 2.5 min. Sample separation was performed on a Poroshell SB-Aq column(50 mm × 4.6 mm, 2.7 μm) using eco-friendly mobile phase consisting of formic acid and ammonium formate aqueous solution at a flow rate of 1.0 m L·min;. The detection wavelength was 260 nm. The developed HPLC method showed good linearity with correlation coefficients of 1.0000 in the test range. Good precision, repeatability and stability of this method were also observed(RSD ≤ 2.81%).The recovery ranged from 91.84%-105.19%(RSD ≤ 2.59%). Compared with reported methods, the current method did not use harmful organic solvent and took only 10.5 min. It obtained a high eco-score of 91 by the "Analytical Eco-Scale" tool. The developed method is eco-friendly and fast, which is suitable for the quality evaluation of Cordyceps and related products.

关键词： Adenosine Inosine Guanosine Uridine Cordyceps

Corrigendum to“Active components of Dengzhan Shengmai ameliorate cognitive impairment by facilitating hippocampal long-term potentiation via the NMDA receptor-mediated Ca2+/CaMKII pathway”[***.14(2024)435–438]

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Journal of pharmaceutical Analysis 2024年第6期14卷 933-933页

作者： Congyu Ma Ning Sheng Jun Wu Lulu Wang Jiandong Jiang Haibo Yu Jinlan Zhang State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing100050China Institute of Medicinal Biotechnology Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing100050China

The authors regret there was an unfortunate error in the reproduction of Fig.1I in the *** the original figure,the fluorescence picture of the positive control drug-ifenprodil was *** authors have corrected Fig.1I and provided the original fluorescence pictures of all groups(seven groups,n=5 for each group)to the editorial ***,the corrected Fig.1I is shown *** authors declare that this correction does not affect the description,interpretation,or the original conclusions of the article.

关键词： correction Active picture