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Impact of Phenanthrene on Denitrification Activity and Transcription of Related Functional Genes in Estuarine and Marine Sediments

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Journal of Ocean University of China 2020年第1期19卷 124-134页

作者： SUN Pengfei BAI Jie LI Kuiran ZHAO Yangguo TIAN Weijun BAI Xiaoyan TIAN Yanzhao college of Environmental science and Engineering Ocean University of ChinaQingdao 266100China The Key Laboratory of Marine Environmental science and Ecology Ministry of EducationOcean University of ChinaQingdao 266100China college of Marine life science Ocean University of ChinaQingdao 266003China

The effects of phenanthrene(Phe)on the denitrification activity and denitrifying genes(narG,nirS and nosZ)were evaluated by dose-response experiments in sediments of Dagu River Estuary(DRE)and Jiaozhou Bay(JZB).The results showed that potential denitrification activity(PDA),N2O,NO3−and NO2−reduction rates of both areas were inhibited with an increase of Phe *** PDA,N2O,NO3−and NO2−reduction rates of both areas was highest and lowest in the control(DRE:0.453,0.427,7.439 and 3.222mgNkg−1 h−1,JZB:0.592,0.555,8.470 and 3.793mgNkg−1 h−1)and highest Phe amended treatments(DRE:0.069,0.001,4.486,and 1.563 mgNkg−1 h−1;JZB:0.114,0.024,5.527 and 2.200 mgNkg−1 h−1).The inhibition rate of PDA was highest,follow by NO2−reduction and then NO3−***,with the increasing of Phe concentrations,total bacteria count and the abundance of denitrifying genes were *** N2O accumulation was promoted with the addition of Phe for both *** on the comparison of EC50 values,denitrifiers harboring three genes were more sensitive to Phe than PDA,and denitrifiers harboring nirS gene were more sensitive,followed by nosZ gene,and then narG ***,according to correlation analysis,the relative abundance of denitrifying genes was much more positively correlated with PDA,NO3−and NO2−reduction than total bacteria *** addition,the denitrification activity and total bacteria count in JZB were more inhibited than that of *** study is useful for understanding the impact of Phe pollution on denitrification in estuary and marine sediments,with profound implications for the management of aquatic ecosystems regarding eutrophication(N-removal)and greenhouse effect.

关键词： phenanthrene effect denitrification denitrifying genes estuarine sediment marine sediment

Remodeling tumor immunosuppressive microenvironment via a novel bioactive nanovaccines potentiates the efficacy of cancer immunotherapy

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Bioactive Materials 2022年第10期7卷 107-119页

作者： Xiaoxue Xie Yi Feng Hanxi Zhang Qingqing Su Ting Song Geng Yang Ningxi Li Xiaodan Wei Tingting Li Xiang Qin Shun Li Chunhui Wu Xiaojuan Zhang Guixue Wang Yiyao Liu Hong Yang Department of Biophysics School of Life Science and TechnologyUniversity of Electronic Science and Technology of ChinaChengdu610054SichuanPR China TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province Hospital of Chengdu University of Traditional Chinese MedicineNo.39 Shi-er-qiao RoadChengdu610072SichuanPR China Key Laboratory of Biorheological science and Technology Ministry of EducationState and Local Joint Engineering Laboratory for Vascular ImplantsBioengineering College of Chongqing UniversityChongqing400030PR China

The clinical outcomes of cancer nanovaccine have been largely impeded owing to the low antigen-specific T cell response rate and acquired resistance caused by the immunosuppressive tumor microenvironment(TME).Here,we reported a tumor acidity-responsive nanovaccine to remodel the immunosuppressive TME and expand the recruitment of tumor infiltrating lymphocytes(TILs)using hybrid micelles(HM),which encapsulated colony stimulating factor 1 receptor(CSF1-R)inhibitor BLZ-945 and indoleamine 2,3-dioxygenase(IDO)inhibitor NLG-919 in its core and displayed a model antigen ovalbumin(OVA)on its surface(denoted as BN@HM-OVA).The bioactive nanovaccine is coated with a polyethylene glycol(PEG)shell for extending nanoparticle *** shell can be shed in response to the weakly acidic tumor *** decrease in size and the increase in positive charge may cause the deep tumor penetration of *** demonstrated that the bioactive nanovaccine dramatically enhance antigen presentation by dendritic cells(DCs)and drugs transportation into M1-like tumor-associated macrophages(TAMs)and tumor cells via size reduction and increasing positive charge caused by the weakly acidic *** bioactive nanovaccine could remodel the immunosuppressive TME into an effector T cells favorable environment,leading to tumor growth inhibition in prophylactic and therapeutic E.G7-OVA tumor ***,combining the bioactive nanovaccine with simultaneous anti-PD-1 antibody treatment leads to a long-term tumor inhibition,based on the optimal timing and sequence of PD-1 blockade against T cell *** research provides a new strategy for the development of efficient cancer immunotherapy.

关键词： Bioactive nanovaccine Cancer immunotherapy IDO inhibitor TAMs TME

Functional Insights of Plant GSK3-like Kinases： Multi-Taskers in Diverse Cellular Signal Transduction Pathways

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Molecular Plant 2015年第4期8卷 552-565页

作者： Ji-Hyun Youn Tae-Wuk Kim Department of life science College of Natural Sciences Hanyang University Seou1133-791 Korea Naturat science Institute Hanyang University Seou1133-791 Korea

The physiological importance of GSK3-like kinases in plants emerged when the functional role of plant GSK3-like kinases represented by BIN2 was first elucidated in the brassinosteroid （BR）-regulated signal transduction pathway. While early studies focused more on understanding how GSK3-like kinases regulate BR signaling, recent studies have implicated many novel substrates of GSK3-like kinases that are involved in a variety of cellular processes as well as BR signaling. Plant GSK3-like kinases play diverse roles in physiological and developmental processes such as cell growth, root and stomatal cell development, flower development, xylem differentiation, light response, and stress responses. Here, we review the progress made in recent years in understanding the versatile functions of plant GSK3-like kinases. Based on the relationship between GSK3-like kinases and their newly identified substrates, we discuss the physiological and biochemical relevance of various cellular signaling mediated by GSK3-like kinases in plants.

关键词： GSK3-like kinases brassinosteroids signal transduction pathway Arabidopsis rice

Overexpression of a Broccoli Defensin Gene BoDFN Enhances Downy Mildew Resistance

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Journal of Integrative Agriculture 2012年第7期11卷 1137-1144页

作者： JIANG Ming HE Cai-ming MIAO Li-xiang ZHANG Yu-chao college of life science Taizhou University Linhai 317000 P.R.China Institute of Horticulture Zhejiang Academy of Agricultural Science Hangzhou 310021 P.R. China

Plant defensins are small, basic cysteine-rich peptides that play important roles in disease resistance. A gene, designated BoDFN, was isolated from Brassica oleracea var. italica. Gene sequence has been submitted to NCBI with an accession no. of HQ436486. Complete coding sequence of BoDFN is 243 bp in length encoding 80 amino acids. Sequence comparison results showed that BoDFN shared high homology with those of crucifer plants and there were only few DNA base differences. RT-PCR results indicated an increase of gene expression in Hyaloperonospora parasitica infected leaves and revealed a significant increase at 24 and 36 h of treatment. A recombinant plasmid, named pBI121-BoDFN, was constructed and introduced into Agrobacterium tumefacien LBA4404. PCR screening for 65 regenerated plantlets, 17 positive plantlets were obtained, PCR screening results revealed that 17 out of 65 regenerated plantlets were positive. Disease resistant identification results indicated that all positive plants showed an increase in resistance to H. parasitica.

关键词： broccoli downy mildew defensin overexpression

Directed Evolution of Insoluble Arabidopsis thaliana Zeta Class Glutathione S-Transferase Mutants for Higher Solubility in Escherichia coli

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Journal of Genetics and Genomics 2013年第6期40卷 319-322页

作者： Yicun Wang Feng Zhang Haiwei Chen Xiwen Chen Defu Chen Laboratory of Molecular Genetics College of Life SciencesNankai University college of life sciences Chifeng CollegeChifeng 024000China

Expression of recombinant protein in Escherichia coli （***） is generally considered as one of the ideal systems to produce proteins for industrial ***,the majority of proteins usually fail to fold into their native ... 详细信息

关键词： Directed Evolution of Insoluble Arabidopsis thaliana Zeta Class Glutathione S-Transferase Mutants for Higher Solubility in Escherichia coli coli

Clay mineralogy of an Eocene fluvial-lacustrine sequence in Xining Basin, Northwest China, and its paleoclimatic implications

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science China Earth sciences 2019年第3期62卷 571-584页

作者： Bin HU Chunxia ZHANG Haibin WU Qingzhen HAO Zhengtang GUO Key Laboratory of Cenozoic Geology and Environment Institute of Geology and Geophysics Chinese Academy of Sciences Institution of Earth science Chinese Academy of Science college of Earth science and Engineering Shandong University of Science and Technology CAS Center for Excellence in life and Paleoenvironment University of Chinese Academy of sciences

The Eocene was marked by significant cooling during which the global climate was transformed from greenhouse to icehouse conditions. Notable coeval events were the India-Asia collision and the retreat of the Paratethys Sea in Asia. The Eocene section of the long and continuous sedimentary succession of the Xining Basin in Northwest China is characterized by red mudstones with intercalated gypsum and muddy-gypsiferous layers. In this study, we conducted a semi-quantitative analysis of the mineralogy of bulk samples and the clay fraction using X-ray diffraction, with the aim of characterizing the Eocene climatic evolution of the northeastern margin of the Tibetan Plateau and inland Asia. We used a new pretreatment method to address the problem of extracting sufficient clay particles from the gypsum and gypsiferous layers. The bulk mineralogy is dominated by quartz, feldspar, calcite, gypsum and dolomite; and the clay mineralogy is dominated by illite, chlorite, and smectite(including irregular mixed-layer illite-smectite(I/S)). The variations of the clay mineral assemblages indicate the occurrence of alternations between warm humid conditions and hot dry conditions, with relatively high humidity during ～52–50,～41.5–39 and ～35–34 Ma. Comparison of the results with the timing of Tibetan Plateau uplift, transgressions and regressions of the Paratethys Sea, and the marine oxygen isotope record suggest that the Eocene climatic evolution of the study region was driven fundamentally by global climate change.

关键词： Eocene Xining Basin Clay minerals Paleoclimate

COP9 signalosome subunit PfCsnE regulates secondary metabolism and conidial formation in Pestalotiopsis fici

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science China(life sciences) 2017年第6期60卷 656-664页

作者： Yanjing Zheng Xiuna Wang Xiaoling Zhang Wei Li Gang Liu Shihua Wang Xiufeng Yan Huixi Zou Wen-Bing Yin Zhejiang Provincial(Wenzhou) Key Lab for Water Environment and Marine Biological Resources Protection College of Life and Environmental Science Wenzhou University State Key Laboratory of Mycology Institute of Microbiology Chinese Academy of Sciences School of life sciences Fujian Agriculture and Forestry University college of life sciences University of Chinese Academy of Sciences

The COP9 signalosome(CSN) is a highly conserved multiprotein complex in all eukaryotes and involved in regulation of organism development. In filamentous fungi, several lines of evidence indicate that fungal development and secondary metabolism(SM) are mediated by the fifth subunit of CSN, called CsnE. Here we uncover a connection with CsnE and conidial formation as well as SM regulation in the plant endophytic fungus Pestalotiopsis fici. A homology search of the P. fici genome with CsnE, involved in sexual development and SM in Aspergillus nidulans, identified PfCsnE. Deletion of PfcsnE resulted in a mutant that stopped conidial production, but the conidia are recovered in a PfcsnE complemented strain. This indicates that PfCsnE is required for the formation of conidia. Secondary metabolite analysis demonstrated that the ΔPfcsnE strain produced more chloroisosulochrin, less ficiolide A production in comparison to wild type(WT). Transcriptome analysis of WT andΔPfcsnE strains indicated that PfcsnE impacts the expression levels of 8.37% of 14,797 annotated genes. Specifically, nine biosynthetic gene clusters(BGCs) were up-regulated and three BGCs were down-regulated by PfCsnE. Our results suggest that PfCsnE plays major roles in SM regulation and conidial development in P. fici.

关键词：次生代谢产物分生孢子拟盘多毛孢调控亚基复合体生物合成基因簇信号

Improved Ant Colony Optimization and Machine Learning Based Ensemble Intrusion Detection Model

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Intelligent Automation & Soft Computing 2023年第4期36卷 849-864页

作者： S.Vanitha P.Balasubramanie Anna University Chennai600025India Department of Computer science and Engineering Kongu Engineering CollegeErode638060India

Internet of things(IOT)possess cultural,commercial and social effect in life in the *** nodes which are participating in IOT network are basi-cally attracted by the cyber-attack *** and identiﬁcation of anomalies in IoT infrastructure is a growing problem in the IoT *** Learning Based Ensemble Intrusion Detection(MLEID)method is applied in order to resolve the drawback by minimizing malicious actions in related botnet attacks on Message Queue Telemetry Transport(MQTT)and Hyper-Text Transfer Proto-col(HTTP)*** proposed work has two signiﬁcant contributions which are a selection of features and detection of *** features are chosen from Improved Ant Colony Optimization(IACO)in the feature selection,and then the detection of attacks is carried out based on a combination of their possible *** IACO approach is focused on deﬁning the attacker’s important features against HTTP and *** the IACO algorithm,the constant factor is calculated against HTTP and MQTT based on the mean function for each *** detection,the performance of several machine learning models are Distance Deci-sion Tree(DDT),Adaptive Neuro-Fuzzy Inference System(ANFIS)and Mahala-nobis Distance Support Vector Machine(MDSVM)were compared with predicting accurate attacks on the IoT *** outcomes of these classiﬁers are combined into the ensemble *** proposed MLEID strategy has effec-tively established malicious *** UNSW-NB15 dataset is used to test the MLEID technique using data from simulated IoT ***,the pro-posed MLEID technique has a greater detection rate and an inferior rate of false-positive compared to other conventional techniques.

关键词： Network intrusion detection system(NIDS) internet of things(IOT) ensemble learning statisticalﬂow features botnet ensemble technique improved ant colony optimization(IACO) feature selection

Effect of Alexandrium tamarense on three bloom-forming algae

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Chinese Journal of Oceanology and Limnology 2010年第4期28卷 940-944页

作者：尹娟谢瑾杨维东李宏业刘洁生 college of life sciences and Technology Jinan University

We investigated the allelopathic properties of Alexandrium tamarense(Laboar) Balech on the growth of Prorocentrum donghaiense Lu,Chattonella marina(Subrahmanyan) Hara et Chihara and Heterosigma akashiwo(Hada) Hada in a laboratory *** examined the growth of ***,***,*** and *** in co-cultures and the effect of filtrates from *** cultures in various growth phases,on the three harmful algal bloom(HAB)-forming *** co-cultures with ***,both *** and *** were dramatically suppressed at high cell densities;in contrast,the growth of *** varied in different inoculative ratios of *** and *** the ratio was 1:1(***:***),growth of *** was inhibited considerably,while the growth of *** was almost the same as that of the control when the ratio was 9:*** growth difference of ***,*** and *** when co-cultured with *** indicated that the allelopathic effect may be one of the important factors in algal competition and phytoplankton succession involving *** addition,the filtrate from *** culture had negative impacts on these three HAB algae,and such inhibition varied with different growth phases of *** in parallel with reported values of PSP toxin content in Alexandrium *** implied that PSP toxin was possibly involved in allelopathy of ***,the rapid decomposition and inactivation of PSP toxin above pH7 weakened this *** studies on the allelochemicals responsible for the allelopathy of *** need to be carried out in future.

关键词：塔玛亚历山大藻藻类东海原甲藻赤潮异弯藻体育发展游艇码头生长阶段化感作用