检索结果-南通市图书馆

A Simple Approach to Bioconjugation at Diverse Levels: Metal-Free Click Reactions of Activated Alkynes with Native Groups of Biotargets without Prefunctionalization

引用

Research 2018年第1期2018卷 172-183页

作者： Xianglong Hu Xueqian Zhao Benzhao He Zheng Zhao Zheng Zheng Pengfei Zhang Xiujuan Shi Ryan T.K.Kwok Jacky W.Y.Lam Anjun Qin Ben Zhong Tang Department of Chemistry Hong Kong Branch of Chinese National EngineeringResearch Center for Tissue Restoration and ReconstructionInstitute of Advanced StudyState Key Laboratory of Molecular NanoscienceDivision of Life Science and Diversion of Biomedical EngineeringTe Hong Kong University of Science and TechnologyClear Water BayKowloonHong KongChina Ministry of Education Key Laboratory of Laser Life Science&Institute of Laser Life Science College of BiophotonicsSouth China Normal UniversityGuangzhou 510631China HKUST-Shenzhen Research Institute Shenzhen 518057China NSFC Center for Luminescence from Molecular Aggregates SCUT-HKUST Joint Research InstituteState Key Laboratory of Luminescent Materials and DevicesSouth China University of TechnologyGuangzhou 510640China

Te efcient bioconjugation of functional groups/molecules to targeted matrix and bio-related species drives the great development of material science and biomedicine,while the dilemma of metal catalysis,uneasy premodifcation,and limited reaction efciency in traditional bioconjugation has restricted the booming development to some ***,we provide a strategy for metal-free click bioconjugation at diverse levels based on activated *** a proof-of-concept,the abundant native groups including amine,thiol,and hydroxyl groups can directly react with activated alkynes without any modifcation in the absence of metal *** this strategy,high-efcient modifcation and potential functionalization can be achieved for natural polysaccharide,biocompatible polyethylene glycol(PEG),synthetic polymers,cell penetrating peptide,protein,fast whole-cell mapping,and even quick diferentiation and staining of Gram-positive bacteria,***,current metal-free click bioconjugation strategy based on activated alkynes is promising for the development of quick fuorescence labeling and functional modifcation of many targets and can be widely applied towards the fabrication of complex biomaterials and future in vivo labeling and detection.

关键词： proof catalysis biomaterials

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nat Cell Biol:研究发现人肝细胞抵抗HCV感染的代谢过程

引用

现代生物医学进展 2016年第35期16卷 I0001-I0002页

作者： Gahl Levy, Naomi Habib, Maria Angela Guzzardi, Daniel Kitsberg, David Bomze, Elishai Ezra, Merav Cohen, Nir Friedman Yaakov Nahmias Gahl Levy, Daniel Kitsberg, David Bomze, Merav Cohen, Nir Friedman Yaakov Nahmias Naomi Habib Maria Angela Guzzardi Elishai Ezra Basak E Uygun Korkut Uygun Martin Trippler Joerg F Schlaak Oren Shibolet Ella H Sklan Joerg Timm Grass Center for Bioengineering Benin School of Computer Science and Engineering The Hebrew University of Jerusalem Jerusalem Israel. Silberman Institute of Life Sciences The Hebrew University of Jerusalem Jerusalem Israel. Klarman Cell Observatory Broad Institute of Harvard and MIT Cambridge Massachusetts USA. Institute of Clinical Physiology National Research Council (CNR) Pisa Italy. Faculty of Engineering Jerusalem College of Technology Jerusalem Israel. Center for Engineering in Medicine Massachusetts General Hospital Boston Massachusetts USA. Department of Gastroenterology and Hepatology University Hospital University Duisburg-Essen Essen Germany. Liver Unit Department of Gastroenterology Tel-Aviv Medical Center and Sackler Faculty of Medicine Tel Aviv Israel. Department of Clinical Microbiology and Immunology Sackler School of Medicine Tel Aviv University Tel Aviv Israel. Institute for Virology Medical Faculty University of D&#x000FC sseldorf D&#x000FC sseldorf Germany.

病毒感染是二十一世纪面临的主要医学挑战之一，从影响全球3％人口的丙肝病毒（HCV）流行病到最近爆发的西尼罗河病毒、寨卡病毒和埃博拉病毒感染。病毒缺乏进行自我复制所需的基础代谢机制。为了解决这个问题，它们劫持它们的宿主的代... 详细信息

病毒感染是二十一世纪面临的主要医学挑战之一，从影响全球3％人口的丙肝病毒（HCV）流行病到最近爆发的西尼罗河病毒、寨卡病毒和埃博拉病毒感染。病毒缺乏进行自我复制所需的基础代谢机制。为了解决这个问题，它们劫持它们的宿主的代谢机制以便完成它们的生命周期和进行增殖。然而，科学家们仍然并没有很好地理解病毒与它们感染的有机体之间在代谢上的相互作用。这主要是因为人基因与代谢过程之间存在复杂的相互作用。

关键词：病毒感染代谢过程 Cell 人肝细胞 Nat HCV 西尼罗河病毒代谢机制

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Impact Factor:1.90 Total flavonoids from Saussurea involucrata attenuate inflammation in lipopolysaccharide-stimulated RAW264.7 macrophages via modulating p65,c-Jun,and IRF3 signaling pathways

引用

Asian Pacific Journal of Tropical biomedicine 2021年第6期11卷 273-284页

作者： Li-Shan Yan Li Wang Brian Chi-Yan Cheng Yu Ding Jing Kong Qing Gao Wang Xiu-Qiong Fu Shuo-Feng Zhang Gan Luo Yi Zhang School of Chinese Materia Medica Beijing University of Chinese MedicineBeijingChina college of Professional and Continuing Education Hong Kong Polytechnic UniversityHong KongChina The First Affiliated Hospital Guangxi University of Chinese MedicineGuangxiChina Centre for Cancer and Inflammation Research School of Chinese MedicineHong Kong Baptist UniversityHong KongChina

Objective:To investigate the anti-inflammatory effects of the total flavonoids from Saussurea involucrata on lipopolysaccharides(LPS)-stimulated murine RAW264.7 macrophages and explore its underlying mechanism of ***:Total flavonoids from Saussurea involucrata were extracted using chromatographic column *** viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide *** production of nitric oxide was detected by Griess assay and the release of cytokines(IL-10 and TNF-α)and chemokines(MCP-1,MIP-1α,and CCL5/RANTES)was determined by ELISA to evaluate the anti-inflammatory activity of total flavonoids from Saussurea ***,nuclear translocation of p65,c-Jun,and IRF3 was detected by immunofluorescence microscopy and Western blotting analysis was performed to determine the expression of related ***:Total flavonoids extracted from Saussurea involucrata were 751.5 mg/g and the content of rutin was 506.5 mg/*** production of inflammatory mediators including nitric oxide,cytokines,and chemokines was effectively inhibited by total flavonoids from Saussurea ***,total flavonoids also suppressed the nuclear translocation of p65,c-Jun,and IRF3 in LPS-stimulated RAW264.7 *** LPS-induced expression of iNOS and COX-2 was remarkably reduced by treatment with total flavonoids from Saussurea ***,total flavonoids decreased the expression levels of p-IKKα/β,p-TBK1,p-p38,p-ERK,p-JNK,p-p65,p-c-Jun,and p-IRF3 in LPS-exposed RAW264.7 ***:Total flavonoids from Saussurea involucrata potentially inhibit the secretion of pro-inflammatory mediators,which may be related to inhibition of p65,c-Jun,and IRF3 signaling pathways in LPS-stimulated RAW264.7 cells.

关键词： Saussurea involucrata Cytokines Proinflammatory Macrophages Flavonoids

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Etoposide,dexamethasone,and pegaspargase with sandwiched radiotherapy in early-stage natural killer/T-cell lymphoma:A randomized phase III study

引用

The Innovation 2023年第3期4卷 70-78页

作者： Huijuan Zhong Shu Cheng Xi Zhang Bing Xu Jiayi Chen Xufeng Jiang Jie Xiong Yu Hu Guohui Cui Juying Wei Wenbin Qian Xiaobing Huang Ming Hou Feng Yan Xin Wang Yongping Song Jianda Hu Yuanhua Liu Xuejun Ma Fei Li Chongyang Wu Junmin Chen Li Yu Ou Bai Jingyan Xu Zunmin Zhu Li Liu Xin Zhou Li Huang Yin Tong Ting Niu Depei Wu Hao Zhang Chaofu Wang Binshen Ouyang Hongmei Yi Qi Song Gang Cai Biao Li Jia Liu Zhifeng Li Rong Xiao Luqun Wang Yujie Jiang Yanyan Liu Xiaoyun Zheng Pengpeng Xu Hengye Huang Li Wang Saijuan Chen Weili Zhao Shanghai Institute of Hematology State Key Laboratory of Medical GenomicsNational Research Center for Translational Medicine at ShanghaiRuijin Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200025China Department of Hematology Xinqiao HospitalChongqing 400037China Department of Hematology First Affiliated Hospital of Xiamen UniversityXiamenFujian 361009China Department of Radiation Oncology Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200025China Department of Nuclear Medicine Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200025China Institute of Hematology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei 430022China Department of Hematology First Affiliated Hospital of Zhejiang University School of MedicineHangzhouZhejiang 310003China Department of Hematology Second Affiliated Hospital of Zhejiang University School of MedicineHangzhouZhejiang 310009China Institute of Hematology Department of HematologySichuan Provincial People’s HospitalChengduSichuan 610072China Department of Hematology Shandong Provincial Key Laboratory of ImmunohematologyQilu HospitalShandong UniversityJinanShandong 250012China Department of Hematology Third Affiliated Hospital of Suzhou UniversityFirst People’s Hospital of ChangzhouChangzhouJiangsu 213004China Department of Hematology Shandong Provincial Hospital Affiliated to Shandong UniversityJinanShandong 250021China Department of Hematology Affiliated Cancer Hospital of Zhengzhou UniversityHenan Cancer HospitalZhengzhouHenan 450003China Fujian Institute of Hematology Fujian Medical University Union HospitalFuzhouFujian 350401China Department of Medical Oncology Jiangsu Cancer HospitalNanjingJiangsu 210009China Department of Medical Oncology Shanghai Cancer CenterFudan UniversityShanghai 200032China Department of Hematology First Affiliated Hospital of Nanchang UniversityNanchan

Methotrexate,etoposide,dexamethasone,and pegaspargase(MESA)with sandwiched radiotherapy is known to be effective for early-stage extranodal natural killer/T-cell lymphoma,nasal type(NKTCL).We explored the efficacy and safety of reduced-intensity,non-intravenous etoposide,dexamethasone,and pegaspargase(ESA)with sandwiched *** multicenter,randomized,phase III trial enrolled patients aged between 14 and 70 years with newly diagnosed early-stage nasal NKTCL from 27 centers in *** were randomly assigned(1:1)to receive ESA(pegaspargase 2,500 IU/m^(2)intramuscularly on day 1,etoposide 200 mg orally,and dexamethasone 40 mg orally on days 2–4)or MESA(methotrexate 1 g/m^(2)intravenously on day 1,etoposide 200 mg orally,and dexamethasone 40 mg orally on days 2–4,and pegaspargase 2,500 IU/m^(2)intramuscularly on day 5)regimen(four cycles),combined with sandwiched radiotherapy.

关键词： radiotherapy killer lymphoma

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nat Cell Biol:研究发现乳腺癌的新型细胞起源

引用

现代生物医学进展 2017年第8期17卷 I0002-I0002页

作者： Leander Blaas, Agneta B. Andersson, Marco Gerling, Alexandra Musch, Dorothee Bornhorst Rune Toftg #x000E5 rd Fabio Pucci, Hendrik A. Messal, E. Josue Ruiz Axel Behrens Iyadh Douagi Bradley Spencer-Dene, Richard Stone Gordon Stamp Richard Mitter Leena Bhaw Abdul K. Sesay Jos Jonkers Ilaria Malanchi Axel Behrens Center for Innovative Medicine (CIMED) Department of Biosciences and Nutrition Karolinska Institutet Novum 141 83 Huddinge Sweden Adult Stem Cell Laboratory The Francis Crick Institute 1 Midland Road London NW1 1AT UK Department of Medicine Center for Hematology and Regenerative Medicine Karolinska Institutet Novum 141 83 Huddinge Sweden Experimental Histopathology The Francis Crick Institute 1 Midland Road London NW1 1AT UK Bioinformatics and Biostatistics The Francis Crick Institute 1 Midland Road London NW1 1AT UK Advanced Sequencing Facility The Francis Crick Institute Mill Hill Laboratory The Ridgeway London NW7 1AA UK Division of Molecular Pathology and Cancer The Netherlands Cancer Institute 1066 CX Amsterdam The Netherlands Tumour-Stroma Interactions in Cancer Laboratory The Francis Crick Institute 1 Midland Road London NW1 1AT UK Faculty of Life Sciences & Medicine King&#x02019 s college London Guy&#x02019 s Campus London SE1 1UL UK

乳腺癌是女性常见的癌症类型之一，长期以来科学家们知道并不是所有的乳腺癌都是相似的，腔体肿瘤主要包含一些和乳腺管内层细胞非常相似的细胞类型，而其它肿瘤所包含的细胞则同乳腺上皮组织外层的细胞相似，研究者认为，这种多样性起... 详细信息

乳腺癌是女性常见的癌症类型之一，长期以来科学家们知道并不是所有的乳腺癌都是相似的，腔体肿瘤主要包含一些和乳腺管内层细胞非常相似的细胞类型，而其它肿瘤所包含的细胞则同乳腺上皮组织外层的细胞相似，研究者认为，这种多样性起源于不同干细胞或祖细胞的突变，

关键词：细胞起源乳腺癌 Cell Nat 细胞类型上皮组织种多样性相似

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：