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Journal of Integrative Agriculture 2013年第11期12卷 1993-2001页

作者： WANG Zhen-yu GAO Xiao-guang ZHANG Ji-hong ZHANG De-quan MA Chang-wei Institute of Agro-Products Processing science and Technology Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-ProductsProcessingMinistry of Agriculture college of food science and nutritional engineering China Agricultural University Department of food science Cornell University

Fatty acid composition of neutral lipids （NLs）, phospholipids （PLs） and free fatty acids （FFAs） from intramuscular fat （IMF）, lipid oxidation and lipase activity in muscle Semimembranosus （SM） and msucle Biceps femoris （BF） of dry-cured Xuanwei ham during the 90-d salting stages were analysed. The salt content increased from 0.34 to 3.52%in BF and from 0.10 to 5.42%in SM during the 90 d salting stage, respectively. PLs of IMF in both BF and SM decreased 54.70%（P〈0.001） and 34.64%（P〈0.05）, furthermore, the saturated fatty acids （SFA）, monounsaturated fatty acids （MUFA） and polyunsaturated fatty acids （PUFA） of PLs in both muscles were hydrolysed almost isochronously. FFAs were increased from 0.46 g 100 g-1 lipids to 2.92 g 100 g-1 lipids in BF at the end of salting, which was lower than SM （from 1.29 g 100 g-1 lipids to 9.70 g 100 g-1 lipids）. The activities of acid lipase, neutral lipase and acid phospholipase all remained active in the 90 d. The thiobarbituric acid reactive substances （TBARS） was slowly increased to 1.34 mg kg-1 muscle in BF and to 2.44 mg kg-1 muscle in SM during the salting stage. In conclusion, the controlled salting process prompted the hydrolysis of PLs of IMF notably and increased the lipid oxidation of muscles within some limits.

关键词： Xuanwei ham intramuscular fat lipid oxidation fatty acid composition salting

Purification and Characterization of Three Alkaline Endopolygalacturonases from a Newly Isolated Bacillus gibsonii

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过程工程学报 2008年第4期8卷 768-773页

作者：李祖明金波张洪勋白志辉薛文通李鸿玉 Teacher's college of Beijing Union university Research Center for Eco-environmental sciences Chinese Academy of SciencesBeijing100085China college of food science&nutritional engineering China Agricultural UniversityBeijing100083China School of Earth and Environmental science University of Adelaide Research Center for Eco-environmental sciences Chinese Academy of Sciences School of Earth and Environmental science University of AdelaideAdelaide college of food science&nutritional engineering China Agricultural University

A newly isolated Bacillus gibsonii,designated as S-2(CGMCC1215),was cultivated for production of alkaline pectinases utilizing sugar beet pulp as growth *** of three alkaline endopolygalacturonases(endoPGs)from the crude pectinases extract was carried out by ultra-filtration,ammonium sulphate fractionation and ion-exchange chromatography,and their enzyme activities *** three purified alkaline endoPGs,designated as S-I,S-II,and S-III,had a molecular weight about38kDa as determined by *** Km value and optimal temperature for optimal enzyme activities of S-I,S-II and S-III were1.2mg/mL and60℃,0.9mg/mL and55℃,1.1mg/mL and60℃,*** best performances were given at an optimal pH10.5,and sodium polygalacturonate was found to be the best *** isoelectric points of S-I,S-II and S-III were5.4,7.4,and8.2,*** of Tween-80and Tween-20and metal ions such as Mg2+and Ca2+stimulated the activity of S-I,S-II and S-III,whereas S-III was inhibited by Ca2+,and Mn2+and Zn2+ions inhibited the activity of the three enzymes.

关键词：净化方法细菌隔离方法果胶酶

Changes in fruit pigment accumulation,chloroplast development,and transcriptome analysis in the CRISPR/Cas9-mediated knockout of Stay-green 1(slsgr1)mutant

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food Quality and Safety 2022年第1期6卷 3-12页

作者： Liqun Ma Ni Zeng Ke Cheng Jinyan Li Keru Wang Chunjiao Zhang Hongliang Zhu college of food science and nutritional engineering China Agricultural UniversityBeijingChina Supervision Inspection&Testing Center of Agricultural Products QualityMinistry of Agriculture and Rural AffairsBeijingChina

The green-flesh(gf)mutant of the tomato fruit ripen to a muddy brown color and has been demonstrated previously to be a loss-of-function ***,we provide more evidence to support this view that S/SGR1 is involved in color change in ripening tomato *** out SISGR1 expression using a clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9 genome editing strategy showed obviously a muddy brown color with significantly higher chlorophyll and carotenoid content compared with wild-type(WT)*** further verify the role of SiSGR1 in fruit color change,we performed transcriptome deep sequencing(RNA-seq)analysis,where a total of 354 differentially expressed genes(124/230 downregulated/upregulated)were identified between WT and ***,the expression of numerous genes associated with photosynthesis and chloroplast function changed significantly when S/SGR1 was knocked *** together,these results indicate that S/SGR1 is involved in color change in ripening fruit via chlorophyll degradation and carotenoid biosynthesis.

关键词： Tomato pigmentaccumulation transcriptome analysis

A mango biological fingerprint anti-counterfeiting method based on Fuzzy C-means clustering

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food Innovation and Advances 2023年第1期2卷 21-27页

作者： Chaoyu Shen Yiqin Zhang Luyao Chen Adele Lu Jia Jiankang Cao Weibo Jiang college of food science and nutritional engineering China Agricultural UniversityBeijing 100083PR China

The anti-counterfeiting of agricultural products plays an important role in protecting the rights and interests of consumers and maintaining the healthy development of the food *** anti-counterfeiting technology mainly relies on anti-counterfeiting features of packaging or labeling,which has the risk of being copied and *** fingerprint anti-counterfeiting is a method of anti-counterfeiting that takes the biological fingerprint of agricultural products as the anti-counterfeiting *** paper aims to take the distribution of lenticels on the surface of mango as a biological fingerprint,and propose a mango biological fingerprint anti-counterfeiting *** the mango ripens,the peel color of mango will change significantly,which will affect the accuracy of anti-counterfeiting *** this paper,the images of ripe mangoes are classified by Fuzzy C-means clustering,and appropriate image enhancement technology is used to highlight the *** results show that the mango biological fingerprint anti-counterfeiting method based on Fuzzy C-means clustering has good accuracy and robustness,and effectively reduces the impact of peel color change on anti-counterfeiting identification during mango *** results support that it is feasible to use the lenticels distribution of mango as a biological *** this paper,a computer vision anti-counterfeiting method based on lenticels distribution is proposed.

关键词： computer Fuzzy method

Troglitazone Induced Apoptosis via PPARγ Activated POX-induced ROS Formation in HT29 Cells

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Biomedical and Environmental sciences 2011年第4期24卷 391-399页

作者： WANG Jing LV XiaoWen SHI JiePing HU XiaoSong DU YuGuo State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China Feed Research Institute Chinese Academy of Agricultural SciencesBeijing 100081China 3.College of Food Science and Nutritional EngineeringChina Agricultural UniversityBeijing 100083China college of food science and nutritional engineering China Agricultural University Beijing 100083 China

Objective In order to investigate the potential mechanisms in troglitazone-induced apoptosis in HT29 cells,the effects of PPARγ and POX-induced ROS were *** [3-（4,5）-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide （MTT） assay,Annexin V and PI staining using FACS,plasmid transfection,ROS formation detected by DCFH staining,RNA interference,RT-PCR RT-QPCR,and Western blotting analyses were employed to investigate the apoptotic effect of troglitazone and the potential role of PPARγ pathway and POX-induced ROS formation in HT29 *** Troglitazone was found to inhibit the growth of HT29 cells by induction of *** this process,mitochondria related pathways including ROS formation,POX expression and cytochrome c release increased,which were inhibited by pretreatment with GW9662,a specific antagonist of PPARγ.These results illustrated that POX upregulation and ROS formation in apoptosis induced by troglitazone was modulated in PPARγ-dependent ***,the inhibition of ROS and apoptosis after POX siRNA used in troglitazone-treated HT29 cells indicated that POX be essential in the ROS formation and PPARγ-dependent apoptosis induced by *** The findings from this study showed that troglitazone-induced apoptosis was mediated by POX-induced ROS formation,at least partly,via PPARγ activation.

关键词： Troglitazone Apoptosis HT29 POX ROS formation PPARγ Cytochrome c release

Integrative metabolomics and transcriptomics analyses reveal pivotal regulatory mechanisms of 1-methylcyclopropene in maintaining postharvest storage quality of‘Fuji’apples

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food Quality and Safety 2023年第1期7卷 49-62页

作者： Lingling Zhang Peiyu Zhang Modi Gao Yi Zhao Chunjiao Zhang Hongliang Zhu college of food science and nutritional engineering China Agricultural UniversityBeijingChina Supervision Inspection&Testing Center of Agricultural Products QualityMinistry of Agriculture and Rural AffairsBeijingChina

In this study,integrative metabolomics and transcriptomics analyses were conducted to investigate the effects of 1-methylcyclopropene(1-MCP)on apple fruit quality during long-term cold *** results showed that 1-MCP(1μL/L)treatment could maintain fruits apparent quality(*** color and firmness),inhibit the increase of rot rate and soluble solids content/titratable acidity ratio,decrease ethylene release,and respiratory intensity during cold storage,and extend shelf ***,1-MCP had long-term effects on the accumulation of many qualities related to metabolite and gene expression in fruits.1-MCP affected genes related to metabolism at the early stage of storage,specifically those of the glycolysis and tricarboxylic acid cycle *** related to the degradation of sucrose,starch,and cellulose were inhibited,and some starch and cellulose synthesis genes were up-regulated by *** from ethylene synthesis and signal transduction being inhibited by 1-MCP,several enzymes(pectinesterase,pectate lyase,polygalacturonase)were involved in pectin degradation,and degradation products of the cell wall(i.e.D-galacturonic acid and D-glucuronic acid)were also strongly inhibited,further maintaining fruit ***,as precursor glutathione(GSH)related to plant resistance,up-regulated the synthase ***,the expression of genes related to cyanoalanine syn-thase and amino acid utilization pathways was suppressed by 1-MCP Collectively,1-MCP could maintain the postharvest quality of apple fruits.

关键词： 1-Methylcyclopropene apple fruit quality metabolomics transcriptomics

Selection of nitrite-degrading and biogenic amine-degrading strains and its involved genes

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food Quality and Safety 2020年第4期4卷 225-235页

作者： Yuxin Li Zhihui Yu Yingchun Zhu Zhixiang Cao college of food science and engineering Shanxi Agricultural UniversityShanxiChina college of food science and Technology Hebei Agricultural UniversityHebeiChina

Objectives:Accumulation of nitrite and biogenic amines(BAs)in fermented meat products is a matter of public health *** study aimed to screen nitrite-degrading and BA-degrading strains from sour porridges and sausages and bacon products in *** and Methods:After screening out 12 strains,the degradation of nitrite,the degradation of BAs,the activities of nitrite-reducing enzymes,and the detection of genes involved in the BAs were assessed by spectrophotometry method with hydrochloric acid naphthalene ethylenediamine,high-performance liquid chromatography,GENMED kit,and polymerase chain reaction,***:Pediococcus pentosaceus labelled M SZ12 and M GC 2,Lactobacillus plantarum labelled M SZ22,and Staphylococcus xylosus labelled Y CC 3 were *** activity of nitrite-reducing enzyme in M SZ22 was 2.663 units/*** degradation rate of total BAs of M SZ22 was 93.24%.The degradation rates of nitrite and BAs of M SZ12 were 86.49%and 37.87%,*** activity of nitrite-reducing enzyme in M SZ12 was up to 1.962 units/mg.M GC 2 showed higher degradation rates of nitrite(89.19%)and Y CC 3 showed higher degradation rates of BAs(36.16%).The genes encoding the multicopper oxidases(suf I/D2EK17)were detected in the four strains,which also did not contain BAs(histidine decarboxylase(hdc),tyrosine decarboxylase(tdc),ornithine decarboxylase(odc),lysine decarboxylase(ldc))formation encoding ***:These four strains(M SZ12,M GC 2,M SZ22,and Y CC 3)are promising candidates to use as starter cultures for nitrite and BAs in fermented sausages.

关键词： nitrite biogenic amines degradation rate strains genetic testing

Cooperative effects of hydrogen sulfide and nitric oxide on delaying softening and decay of strawberry

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International Journal of agricultural and Biological engineering 2014年第6期7卷 114-122页

作者： Zhang Chang Shi Jingying Zhu Liqin Li Changle Wang Qingguo college of food science and engineering Shandong Agricultural UniversityTaian 271018China college of food science and engineering Jiangxi Agricultural UniversityNanchang 330045China

Hydrogen sulfide(H2S)and nitric oxide(NO)as two signal molecules play prominent roles in plant response to biotic *** combination effects of these two molecules on ripening and decay of postharvest strawberry were *** were first dipped in distilled water,0.8 mmol/L NaHS solution,5μmmol/L sodium nitroprusside(SNP)solution,and combinative solutions of 0.8 mmol/L NaHS and 5μmmol/L SNP for 10 min,respectively,and then stored at 20°*** showed that the effects of the co-treatment of 0.8 mmol/L NaHS solution and 5μmmol/L SNP solution were superior to that of other *** former could suppress the percentage of fruit decay,inhibit respiration rate,maintain the original crust color,and slow down the decrease of firmness and the change of relative conductivity,which preserved the fruit *** investigation showed that co-treatment of hydrogen sulfide and nitric oxide maintained higher activities of chitinase(CHI)and beta-1,3-glucanase(GNS),and lowered activities of pectin methylesterase(PME),polygalacturonase(PG)and endo-β-1,4-glucanase(EGase).The study indicated that the synergistic function of H2S and NO could effectively prolong the shelf life and reduce the decay rate of harvested strawberry.

关键词： hydrogen sulfide nitric oxide strawberry enzyme activities shelf life

Transcriptomics integrated with metabolomics reveals the mechanism of CaCl_(2)-HCl electrolyzed water-induced glucosinolate biosynthesis in broccoli sprouts

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food science and Human Wellness 2024年第2期13卷 801-812页

作者： Cui Li Shuhui Song Yanan He Haijie Liu college of food science and nutritional engineering China Agricultural UniversityBeijing 100083China Institute of Agri-food Processing and Nutrition Beijing Academy of Agriculture and Forestry SciencesBeijing 100097China

Glucosinolates are important phytochemicals in *** investigated the effect of CaCl_(2)-HCl electrolyzed water(CHEW)on glucosinolates biosynthesis in broccoli *** results showed that CHEW treatment significantly decreased reactive oxygen species(ROS)and malondialdeh yde(MDA)contents in broccoli *** the the 8^(th)day,compared to tap water treatment,the the total glucosinolate content of broccoli sprouts with CHEW treatment increased by 10.6%and calcium content was dramatically enhanced from 14.4 mg/g DW to 22.7 mg/g *** transcriptome and metabolome analyses revealed that CHEW treatment activated ROS and calcium signaling transduction pathways in broccoli sprouts and they interacted through MAPK ***,CHEW treatment not only promoted the biosynthesis of amino acids,but also enhanced the expression of structural genes in glucosinolate synthesis through transcription factors(MYBs,bHLHs,WRKYs,etc.).The results of this study provided new insights into the regulatory network of glucosinolates biosynthesis in broccoli sprouts under CHEW treatment.

关键词： Broccoli sprouts CaCl_(2)-HCl electrolyzed water Glucosinolates Transcriptomics Metabolomics

The amino acids differences in epitopes may promote the different allergenicity of ovomucoid derived from hen eggs and quail eggs

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food science and Human Wellness 2023年第3期12卷 861-870页

作者： Mengzhen Hao Shuai Yang Shiwen Han Huilian Che Key Laboratory of Precision Nutrition and food Quality Key Laboratory of Functional DairyMinistry of EducationCollege of Food Science and Nutritional EngineeringChina Agricultural UniversityBeijing 100083China

Quail egg ovomucoid can inhibit activation of basophils and eosinophils,while hen egg ovomucoid has been shown to be a major allergen,named Gal d *** present,the differences in structure and function between two ovomucoid are *** found the homology of ovomucoid in quail eggs and hen eggs reached77%.Compared with hen egg ovomucoid,the distribution of secondary structure was different in AA52-53,AA57-58,AA66-68,AA71-72,AA131-133,AA139-140,AA157-159 and *** 9 epitopes of egg ovomucoid,there were different amino acids from quail egg ovomucoid in 8 *** quail egg ovomucoid had trypsin inhibition activity and quail egg ovomucoid didn't specifically bind to serum of eggs allergic *** egg ovomucoid can significantly inhibit RBL-2 H3 cells degranulation and protect cells morphology to a certain extent,indicating quail egg ovomucoid can inhibit cells activation and have potential anti-allergic effects,which is related to trypsin inhibitory *** difference in sensitization compare to hen egg ovomucoid may be due to amino acids differences affecting protein structure by changing antigenic epitopes.

关键词： Quail egg Hen egg Ovomucoid Epitope Degranulation