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Mapping of QTL conferring leaf rust resistance in Chinese wheat lines W014204 and Fuyu 3 at adult plant stage

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Journal of Integrative Agriculture 2016年第1期15卷 18-28页

作者： QI Ai-yong ZHANG Pei-pei ZHOU Yue YAO Zhan-jun LI Zai-feng LIU Da-qun Department of plant Pathology College of Plant Protection Agricultural University of Hebei Department of Biochemistry Baoding University college of agronomy Agricultural University of Hebei

Wheat leaf rust is a destructive foliar disease of common wheat （Triticum aestivum L.） worldwide. The most effective, economical s to control the disease is growing resistant cultivars with adult plant resistance （APR）. The Chinese wheat tines W014204 and Fuyu 3 showed high leaf rust resistance in the field. To identify leaf rust APR genes in the two lines, two mapping populations with 215 and 163 F2：3 lines from the crosses W014204/Zhengzhou 5389 and Fuyu 3/Zhengzhou 5389, respectively, were phenotyped for leaf rust severities during the 2010-2011, 2011-2012 and 2012-2013 cropping seasons in the field at Baoding, Hebei Province, China. A total of 1 215 SSR markers were used to identify the quantitative trait loci （QTLs） for leaf rust APR in the two populations. In the W014204/Zhengzhou 5389 population, three QTLs were detected and designated as ***-lBL.1, ***-2BS.1 and ***-7DS, and explained 2.9-8.4, 11.5-38.3 and 8.5-44.5% of the phenotypic variance, respectively; all the resistance alleles at these loci were derived from W014204. In the Fuyu 3/Zhengzhou 5389 population, three QTLs, ***-lBL.2, ***-2BS.2 and ***-7BL, explained 12.0-19.2, 22.3-38.9 and 4.1-4.3% of the phenotypic variance, respectively, and all resistance alleles were contributed by Fuyu 3. Based on chromosome positions of closely linked markers, both ***-lBL. 1 and ***-lBL.2 are Lr46, and ***-7DS is Lr34. ***-7BL was mapped on chromosome 7BL near to Lr68 and they are likely the same gene. Based on chromosome positions, pedigree and field reactions, the two 2BS QTLs are different from all the known APR genes and are likely to be newAPR QTL for leaf rust. These QTLs and their closely linked markers are potentially useful for improving leaf rust resistance in wheat breeding.

关键词： Triticum aestivum Puccinia triticina APR SSR marker

The effects of Trichoderma on preventing cucumber fusarium wilt and regulating cucumber physiology

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Journal of Integrative Agriculture 2019年第3期18卷 607-617页

作者： LI Mei MA Guang-shu LIAN Hua SU Xiao-lin TIAN Ying HUANG Wen-kun MEI Jie JIANG Xi-liang Institute of plant protection Chinese Academy of Agricultural Sciences college of agronomy Heilongjiang Bayi Agricultural University

In our previous studies, we identified 3 Trichoderma strains with anti-Fusarium oxysporum activity, including T. asperellum 525, T. harzianum 610, and T. pseudokoningii 886. Here, we evaluated the effects of these 3 Trichoderma strains on preventing cucumber fusarium wilt through pot culture and greenhouse culture experiments. All 3 Trichoderma strains demonstrated higher control effects toward cucumber fusarium wilt than previous studies, with efficacies over 78%. Additionally, inoculation with the 3 Trichoderma strains significantly promoted the quality and yield of cucumbers. Among the 3 strains, Trichoderma 866 was the most effective, with disease control efficacy of 78.64% and a cucumber yield increase of 33%. Furthermore, seedlings inoculated with Trichoderma exhibited significantly increased measures of plant height, stem diameter, leaf area, aboveground fresh weight, underground fresh weight, chlorophyll content, and nitric nitrogen content, as well as the activities of several stress-resistance enzymes, including superoxide dismutase(SOD), peroxidase(POD), catalase(CAT), polyphenol oxidase(PPO), and ascorbate oxidase(AAO). In addition, the plants inoculated with Trichoderma showed decreased cell membrane permeability and malondialdehyde(MDA) content in the leaves. Together, our results suggest that T. asperellum 525, T. harzianum 610, and T. pseudokoningii 886 inoculations inhibit F. oxysporum infection, stimulate the metabolism in cucumbers, and enhance the activities of stress-resistance enzymes, which consequently promote the growth of cucumber plants, prevent cucumber fusarium wilt, and improve the yield and quality of cucumbers. T. harzianum is a commonly used biocontrol fungus, while few studies have focused on T. asperellum or T. koningense. In this study, strains of T. asperellum and T. pseudokoningii showed excellent plant disease prevention and growth promoting effects on cucumber, indicating that they also have great potential as biocontrol fungi

关键词： Trichoderma cucumber fusarium wilt physicochemical features control effect Fusarium oxysporum f. sp. cucumerinum Owen

Molecular characterization of glutaredoxin 2 from Ostrinia furnacalis

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Integrative Zoology 2013年第1期8卷 30-38页

作者： Shiheng AN Ying ZHANG Tiantian WANG Meihao LUO Chaohai LI college of plant protection Henan Agricultural UniversityZhengzhouChina college of agronomy Henan Agricultural UniversityZhengzhouChina

Glutaredoxins(GRXs)play very important roles in maintaining intracellular redox *** the present study,the full-length cDNA sequence encoding GRX2,named OfurGRX2(GenBank accession ***393246),was obtained from Ostrinia furnacalis,using reverse transcription polymerase chain reaction and rapid amplification of cDNA *** analysis revealed that the open reading frame of OfurGRX2 consists of 351 nucleotides encoding 116 amino acid residues with a predicted molecular weight of 12.6 *** research revealed that OfurGRX2 shares a common active site,CPYC/CPFC,with other insect *** profiles revealed that OfurGRX2 is a ubiquitous gene expressed in insect heads,fat bodies,epidermises,mid guts and *** OfurGRX2 transcript peaked in 36-h larvae of 4th instars,and then suddenly declined in the molting *** treatment experiments revealed that 20-hydroxyecodyson(20e)significantly induces the expression of the OfurGRX2 transcript,whereas juvenile hormone(JH)counteracts 20e *** stress factors(including starvation,ultraviolet light,mechanical injury,Escherichia coli exposure,and high and low temperatures)dramatically induced OfurGRXGRX2 transcript expression,which confirmed for the first time that GRX2 play important roles in insecta during exposure to adverse environments.

关键词： gene regulation glutaredoxin Ostrinia furnacalis real-time polymerase chain reaction

Bioinformatics and Functional Analysis of High Oleic Acid-Related Gene GmSAM22 in Soybean [Glycine max (L.) Merr.]

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Phyton-International Journal of Experimental Botany 2023年第2期92卷 501-519页

作者： Shuo Qu Qi Cai Huimin Cui Lamboro Abraham Yaolei Jiao Guilong Ma Piwu Wang college of agronomy Jilin Agricultural UniversityChangchun1300118China college of plant protection Agricultural UniversityChangchun1300118China

High yield,high quality,stable yield,adaptability to growth period,and modern mechanization are the basic requirements for crops in the 21st *** oleic acid is a natural unsaturated fatty acid with strong antioxidant properties and *** as a safe fatty acid,it has the ability to successfully prevent cardiovascular and cerebrovascular *** the fatty acid composition of soybean seeds,can not only speed up the breeding process of high-quality high-oil and high-oleic soybeans,but also have important significance in human health,and provide the possibility for the development of soybean oil as a new energy ***,the aim of this study was to analyze the high oleic acid elated gene GmSAM22 in *** this research the soybean oleic acid-related gene GmSAM22 was screened out by Genome-wide association analysis,a 662 bp fragment was acquired by specific PCR amplification,and the pMD18T cloning vector was linked by the use of a seamless cloning *** analysis of the signal peptide prediction,subcellular localization,protein hydrophobicity,transmembrane region analysis,a phosphorylation site,protein secondary and tertiary structure and protein interaction analysis of the protein encoded by the SAM22 gene was carried *** plasmid of the gene editing vector is *** overexpression vector was transformed from pCAMBIA3301 as the base vector,and overexpression vector were *** plants were obtained by genetic transformation by the pollen tube channel *** quantitative PCR was performed on the T2 generation plants to detect the relative expression levels in different *** Blot was used to detect the presence of hybridization *** genes BAR,35S,and NOS in plants were identified by conventional PCR.10 seeds with high and low oleic acid content were chosen for quantitative PCR identification,and finally,the concentration and morphology of soybean fatty acids were

关键词： Soybean SAM22 gene oleic acid cloning CRISPR/Cas9 expression vector

Development of SSR Markers for a Phytopathogenic Fungus,Blumeria graminis ***,Using a FIASCO Protocol

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Journal of Integrative Agriculture 2014年第1期13卷 100-104页

作者： WANG Meng XUE Fei YANG Peng DUAN Xia-yu ZHOU Yi-lin SHEN Chong-yao ZHANG Guo-zhen WANG Bao-tong Institute of plant protection Chinese Academy of Agricultural Sciences Department of plant Pathology China Agricultural University/Key Laboratory of Plant PathologyMinistry of Agriculture Huai'an Entry-Exit Inspection and Quarantine Bureau college of agronomy Shihezi University college of plant protection Northwest A&F University

Simple sequence repeats （SSR） have been widely used as molecular markers due to their abundance and high polymorphism, However, up to now, the SSR markers had not been developed in the obligate biotrophic phytopathogenic fungus, Blumeria graminis ***. tritici. From （AC）10 and （AG）10 enriched genomic libraries for Bgt, 25 primer pairs were designed using the FIASCO （fast isolation by AFLP of sequences containing repeats） protocol. Five primer pairs exhibited polymorphism with allelic diversity from two to seven alleles and produced 29 alleles in a survey of 90 isolates collected from six provinces （cities） in China, while the others displayed monomorphic. Levels of observed heterozygosity ranged from 0.000-0.044 （mean 0.025） and expected heterozygosity ranged from 0.297-0.816 （mean 0.538）. These molecular markers provide a novel source to genetic diversity assays and to genetic and physical mapping ofBgt. SSR markers of Bgt need to be further explored.

关键词： Blumeria graminis f.sp. tritici microsatellite SSR FIASCO

Conidia of one Fusarium solani isolate from a soybean-production field enable to be virulent to soybean and make soybean seedlings wilted

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Journal of Integrative Agriculture 2018年第9期17卷 2042-2053页

作者： ZHENG Na ZHANG Liu-ping GE Feng-yong HUANG Wen-kun KONG Ling-an PENG De-liang LIU Shi-ming Institute of plant protection Chinese Academy of Agricultural Sciences college of plant protection Hunan Agricultural University

Fusarium is usually thought to cause soybean root rot, which results in a large quantity of annual yield loss in soybean production, by its secretions including Fusarium toxins and cell wall degrading enzymes, but not by the conidia themselves that do not underlie any virulence so far. Here we report that the conidia of one Fusarium solani isolate are able to be virulent to soybean and make soybean seedlings wilted alone. We isolated them from the wilted plants in a soybean-production field and molecularly identified 17 Fusarium isolates through phylogenetic analysis. Of them, except for one isolate that showed diversity of virulence to different soybeans （virulent to one soybean whereas avirulent to another soybean）, the others were all virulent to the two tested soybeans： both conidia cultures and secretions could make soybean seedlings wilted at 5 days post infection, and their virulence had dosage effects that only conidia cultures of at least 5×10^6 conidia mL-1 could show virulence to soybean; however, the sole conidia of the F. solani isolate #4 also exhibited virulence to soybean and could make soybean seedlings wilted. Finally, we developed the specific cleaved amplified polymorphic sequences （CAPS） markers to easily differentiate Fusarium isolates. The isolate #4 in this work will likely be used to investigate the new mechanism of virulence of Fusarium to soybean.

关键词： Fusarium soybean root rot conidia secretions virulence cleaved amplified polymorphic sequences (CAPS) marker

Selectivity and sublethal effects of some frequently-used biopesticides on the predator Cyrtorhinus lividipennis Reuter(Hemiptera: Miridae)

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Journal of Integrative Agriculture 2019年第1期18卷 124-133页

作者： DAI Wei LI Yao ZHU Jun GE Lin-quan YANG Guo-qing LIU Fang college of Horticulture and plant protection Yangzhou University

The green miridbug,Cyrtorhinus lividipennis,is an important predator of the rice brown planthopper,Nilaparvata *** this study,the selective toxicity of seven commercial biopesticides for *** was examined under laboratory conditions; abamectin was the most selective to ***,followed by matrine and ***,rotenone,Bacillus thuringiensis and Beauveria bassiana showed less selectivity for ***,matrine,abamectin and azadirachtin were selected for sublethal assessments with respect to *** due to their high toxicities to *** treated with sublethal concentrations(LC10 and LC20) of the three biopesticides could distinguish volatiles released from healthy and ***-infested plants indicating that the biopesticides tested did not affect the foraging ability of surviving *** decreased the consumption capability of *** when the densities of *** were 20,30,40 and 50 insects per *** concentration treatment did not impact the pre-oviposition period or egg hatchability of ***,the fecundity of *** exposed to azadirachtin and abamectin increased by 27–41% compared to the untreated *** summary,abamectin or matrine together with *** could be considered an effective,sustainable pest management strategy for rice.

关键词： biopesticides Cyrtorhinus lividipennis selective toxicity sublethal effects

Assessment of Rice Cultivars in China for Field Resistance to Aphelenchoides besseyi

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Journal of Integrative Agriculture 2014年第10期13卷 2221-2228页

作者： FENG Hui WEI Li-hui LIN Mao-song ZHOU Yi-jun college of plant protection Nanjing Agricultural University Institute of plant protection Jiangsu Academy of Agricultural Sciences

The effect ofAphelenchoides besseyi on 27 cultivars of rice （23japonica and 4 indica） was assessed in the field for two seasons during 2010 and 2011. The vigorous pathogenic nematodes culturing on Botrytis cinerea were used for this experiment. Inoculation was carried out at the tilling stage; the growth parameters and nematode population were recorded at the end of growth of rice plants. The results showed that the cultivars differed in their response to infection. Most of cultivars were lack of the characteristic symptom of white tip, which was seen less frequently than the other two symptoms, namely small grains and erect panicles; moreover, the expression of symptoms was probably hereditary. The infection lowered the values of all the measured biological parameters, namely length of the stem and of the panicle, the number of filled grains per panicle, and 100-grain weight, in all the cultivars. The final nematode population indicated that the threshold of economic damage had also been exceeded in 10 cultivars, and none of them was immune. Three japonica cultivars proved most vulnerable whereas Tetep, an indica type, showed a level of resistance potentially useful in controlling A. besseyi.

关键词： Aphelenchoides besseyi rice symptom expression yield loss resistance assessment

Comparative Morphology and Morphometry of Six Biotypes of Bemisia tabaci (Hemiptera: Aleyrodidae) from China

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Journal of Integrative Agriculture 2013年第5期12卷 846-852页

作者： LI Jing-jing TANG Qing-bo BAI Run-e LI Xiao-min JIANG Jin-we ZHAI Qing YAN Feng-ming college of plant protection Henan Agricultural University

Morphology and morphometry of six biotypes （B, Q, Cv, ZHJ-1, ZHJ-2 and ZHJ-3） of Bemisia tabaci （Gennadius） from China on cotton plants were studied by using microscopes. Nymphal body sizes and characters were measured and observed, especially on the 4th instar （pupal case）, including the vasiform orifice, operculum, lingula, length and thickness of anterior and posterior wax margins, width of thoracic tracheal combs. Adult characters of both sexes were investigated including the body size, compound eyes, antennal segments, vasiform orifice, hind tarsi and genitals. The results indicated that differences of some morphological characters or morphometrics were significant among the six biotypes of B. tabaci in China：（1） Pupal sizes of the exotic biotypes （B and Q） were significantly larger than the indigenous biotypes with the following order as B〉Q〉ZHJ-I〉Cv〉ZHJ-3〉ZHJ-2; （2） for both male and female adults, sizes of all characters investigated in the invading biotypes （B and Q）, especially B, were much larger than those of the indigenous ones.

关键词： Bemisia tabaci biotype morphology morphometry nymph adult size wax margin vasiform orifice antennalsegments