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Landscape ecological risk assessment and its driving factors in the Weihe River basin,China

Journal of Arid Land 2024年第5期16卷 603-614页

作者： CHANG Sen WEI Yaqi DAI Zhenzhong XU Wen WANG Xing DUAN Jiajia ZOU Liang ZHAO Guorong REN Xiaoying fENG Yongzhong college of agronomy Northwest A&F UniversityYangling 712100China

Weihe River basin is of great significance to analyze the changes of land use pattern and landscape ecological risk and to improve the ecological basis of regional *** on land use data of the Weihe River basin in 2000,2010,and 2020,with the support of Aeronautical Reconnaissance Coverage Geographic Information System(ArcGIS),GeoDa,and other technologies,this study analyzed the spatial-temporal characteristics and driving factors of land use pattern and landscape ecological *** showed that land use structure of the Weihe River basin has changed significantly,with the decrease of cropland and the increase of forest land and construction *** the past 20 a,cropland has decreased by 7347.70 km2,and cropland was mainly converted into forest land,grassland,and construction *** fragmentation and dispersion of ecological landscape pattern in the Weihe River basin were improved,and land use pattern became more ***,landscape ecological risk of the Weihe River basin has been *** landscape ecological risk area decreased by 19,177.87 km2,high landscape ecological risk area decreased by 3904.35 km2,and moderate and low landscape ecological risk areas continued to *** is worth noting that landscape ecological risks in the upper reaches of the Weihe River basin are still relatively serious,especially in the contiguous areas of high ecological risk,such as Tianshui,Pingliang,Dingxi areas and some areas of Ningxia Hui Autonomous *** ecological risk showed obvious spatial dependence,and high ecological risk area was *** the driving factors,population density,precipitation,normalized difference vegetation index(NDVI),and their interactions are the most important factors affecting the landscape ecological risk of the Weihe River *** findings significantly contribute to our understanding of the ecological dynamics in the Weihe River basin,providing crucial insights for sustainable mana

关键词： land use ecological risk spatiotemporal distribution geographic detector driving factors

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Landscape of Sequence Variations in Homologous Copies of fAD2 and fAD3 in Rapeseed(Brassica napus L.)Germplasm with High/Low Linolenic Acid Trait

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Phyton-International Journal of Experimental Botany 2024年第3期93卷 627-640页

作者： Haoxue Wu Xiaohan Zhang Xiaoyu Chen Kang Li Aixia Xu Zhen Huang Jungang Dong Chengyu Yu college of agronomy Northwest A&F UniversityYangling712100China

Genetic manipulation(either restraint or enhancement)of the biosynthesis pathway ofα-linolenic acid(ALA)in seed oil is an important goal in Brassica napus *** is a tetraploid plant whose genome often har-bors four and six homologous copies,respectively,of the two fatty acid desaturases fAD2 and fAD3,which con-trol the last two steps of ALA biosynthesis during seed oil *** this study,we compared their promoters,coding sequences,and expression levels in three high-ALA inbred lines 2006L,R8Q10,and YH25005,a low-ALA line A28,a low-ALA/high-oleic-acid accession SW,and the wildtype *** expression levels of most fAD2 and fAD3 homologs in the three high-ALA accessions were higher than those in ZS11 and much higher than those in A28 and *** three high-ALA accessions shared similar sequences with the pro-moters and CDSs of BnfAD3.C4 and *** A28 and SW,substitution of three amino acid residues in BnfAD2.A5 and BnfAD2.C5,an absence of BnfAD2.C1 locus,and a 549 bp long deletion on the BnfAD3.A3 promoter were *** proﬁle of BnfAD2 mutation in the two low-ALA accessions A28 and SW is different from that reported in previous *** mutations in BnfAD3 in the high-ALA accessions are reported for theﬁrst *** identifying the sites of these mutations,we provide detailed information to aid the design of mole-cular markers for accelerated breeding schemes.

关键词： Brassica napus linolenic acid fAD2 fAD3 promoter coding sequences mutation

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N-cycle gene abundance determination of N mineralization rate following re-afforestation in the Loess Plateau of China

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Soil Ecology Letters 2024年第1期6卷 97-106页

作者： Yaping Zhao Yuqing Zhao Shuohong Zhang Yulin Xu Xinhui Han Gaihe Yang Chengjie Ren college of agronomy Northwest A&F UniversityYangling 712100China Shaanxi Engineering Research Center of Circular Agriculture College of AgronomyNorthwest A&F UniversityYangling 712100China

Afforestation effectively improved soil microbial communities and significantly increased soil nitro-gen mineralization rate(Rm).Soil microorganisms drive Rm by regulating soil N-cycling *** nitrification genes had a major effect on soil Rm than denitrification genes after *** the function of forest ecosystems requires an understanding of the mechanism of soil nitrogen ***,it remains unclear how soil N-cycling genes drive soil nitrogen mineralization during *** this study,we collected soil samples from a chrono-sequence of 14,20,30,and 45 years of Robinia pseudoacacia L.(RP14,RP20,RP30,and RP45)with a sloped farmland(fL)as a *** metagenomic sequencing analysis,we found significant changes in the diversity and composition of soil microbial communities involved in N-cycling along the afforestation time series,with afforestation effectively increasing the diversity(both alpha and beta diversity)of soil microbial *** conducted indoor culture experiments and analyzed correlations,which revealed a significant increase in both soil nitrification rate(Rn)and soil nitrogen mineralization rate(Rm)with increasing stand ***,we found a strong correlation between soil Rm and soil microbial diversity(both alpha and beta diversity)and with the abundance of soil N-cycling *** least squares path modeling(PLS-PM)analysis showed that nitrification genes(narH,narY,nxrB,narG,narZ,nxrA,hao,pmoC-amoC)and denitrification genes(norB,nosZ,nirK)had a greater direct effect on soil Rm compared to their effect on soil microbial *** results reveal the relationships between soil nitrogen mineralization rate and soil microbial communities and between the mineralization rate and functional genes involved in N-cycling,in the context of Robinia pseudoacacia *** on the Loess *** study enriches the understanding of the effects of microorganisms on soil nitrogen mineraliza

关键词： afforestation soil N mineralization rate denitrification genes nitrification genes the Loess Plateau

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Selection and application of four QTLs for grain protein content in modern wheat cultivars

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Journal of Integrative Agriculture 2024年第8期23卷 2557-2570页

作者： Zihui Liu Xiangjun Lai Yijin Chen Peng Zhao Xiaoming Wang Wanquan Ji Shengbao Xu State Key Laboratory of Crop Stress Biology for Arid Areas/college of agronomy Northwest A&F UniversityYangling 712100China

The grain protein content(GPC)is the key parameter for wheat grain nutritional *** study conducted a resampling GWAS analysis using 406 wheat accessions across eight environments,and identified four previously reported GPC *** analysis of 87 landraces and 259 modern cultivars revealed the loss of superior GPC haplotypes,especially in Chinese *** haplotypes were preferentially adopted in different agroecological zones and had broad effects on wheat yield and agronomic *** GPC QTLs did not significantly reduce yield,suggesting that high GPC can be achieved without a yield *** results of this study provide a reference for future GPC breeding in wheat using the four identified QTLs.

关键词： breeding grain protein content haplotype selection and application wheat

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Endoplasmic reticulum stress improved chicken tenderness,promoted apoptosis and autophagy during postmortem ageing

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food Science and Human Wellness 2024年第4期13卷 2141-2151页

作者： Yuwei Chai Lin Chen Shuya Xiang Linxuan Wu Xuebo Liu Jun Luo Xianchao feng college of food Science and Engineering Northwest A&F UniversityYangling 712100China college of Animal Science and Technology Northwest A&F UniversityYangling 712100China

In this study,endoplasmic reticulum(ER)stress inducer tunicamycin(TM)and inhibitor 4-phenylbutyric acid(4-PBA)were used to treat postmortem chicken breast muscle to investigate changes in tenderness and effects on apoptosis and autophagy during 5 days ***-induced ER stress reduced shear force,enhanced myofibril fragmentation index(MfI),disrupted myofibril structure,increased desmin degradation,and activatedμ-calpain and *** addition,TM-induced ER stress increased the expression of Bax,Bim,and cytochrome c,and decreased the expression of Bcl-x ***,TM-induced ER stress improved the conversion of LC3I to LC3II,raised the expression of Beclin-1,and decreased the expression of p62,PI3K,and m *** opposite results were observed after 4-PBA *** results suggested that ER stress could improve chicken tenderness,promote apoptosis and autophagy during chicken postmortem ageing.

关键词： Endoplasmic reticulum stress Tenderness Apoptosis Autophagy Chicken

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Grain-filling strategies of wheat of contrasting grain sizes under various planting patterns and irrigation levels

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The Crop Journal 2024年第3期12卷 897-906页

作者： Zimeng Liang Jingyi feng Jiayu Li Yangyang Tang Tiankang He Vinay Nangia Yang Liu college of agronomy Northwest A&F UniversityYangling 712100ShaanxiChina International Center for Agricultural Research in the Dry Areas Rabat 999055Morocco

In a study comparing grain filling and yield in a large-and a small-grain-size wheat cultivar under two planting patterns and two irrigation regimes,plastic-covered ridge and furrow planting with sprinkler irrigation increased grain filling and yield in both *** largest contributors to grain yield were an extended active grain-filling period in Shuangda 1 and an increased mean grain-filling rate in XN538.

关键词： Plastic-covered ridge and furrow cultivation Sprinkler irrigation Wheat Grain size Grain filling

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Arginine promotes seed energy metabolism,increasing wheat seed germination at low temperature

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The Crop Journal 2024年第4期12卷 1185-1195页

作者： Jiayu Li Zhiyuan Li Yangyang Tang Jianke Xiao Vinay Nangia Yang Liu college of agronomy Northwest A&F UniversityYangling 712100ShaanxiChina International Center for Agricultural Research in the Dry Areas Rabat 999055Morocco

Low temperatures during germination inhibit seed growth,lead to small and weak seedlings,and significantly reduce the wheat *** the adverse effects of low temperature on wheat seed germination is highly important for achieving high and stable wheat *** this study,Tongmai 6(insensitive)and Zhengmai 113(sensitive),which have different low-temperature sensitivities during germination were treated with low temperature during *** transcriptome,metabolome and physiological data revealed that low temperature decreased the germination rate,downregulated the expression of a large number of genes involved in regulating glycometabolism,and inhibited carbon,nitrogen(especially amino acids)and energy metabolism in the *** content increased at low temperature,and its increase in the low-temperature-tolerant variety was significantly greater than that in the sensitive *** priming experiment showed that treatment with an appropriate concentration of arginine improved the seed germination *** conversion of starch to soluble sugar significantly increased under exogenous arginine conditions,the content of key metabolites in energy metabolism increased,and the utilization of ATP in the seeds *** together,arginine priming increased seed germination at low temperature by relieving inhibition of seed carbon and nitrogen metabolism and improving seed energy metabolism.

关键词： Low temperature Seed germination Arginine Energy metabolism Wheat

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Selenium nanoparticles in aquaculture:Unique advantages in the production of Se-enriched grass carp(Ctenopharyngodon idella)

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Animal Nutrition 2024年第1期16卷 189-201页

作者： Chao Zhu Zifang Wu Qimin Liu Xiaolin Wang Lijuan Zheng Shuyang He fangxia Yang Hong Ji Wuzi Dong college of Animal Science and Technology Northwest A&F UniversityYanglingShaanxi712100China college of forestry Northwest A&F UniversityYanglingShaanxi712100China Biology Research Centre of Qin Mountains Wildlife Northwest A&F UniversityYanglingShaanxi712100China

The production of selenium-enriched fish can contribute to alleviating selenium deficiency in human ***,it is still unclear which selenium source,as an additive,can efficiently and costeffectively produce high-quality selenium-enriched *** study evaluated the effects of selenium nanoparticles(SeNP),selenite,and selenomethionine(SeMet)on the growth,antioxidant capacity,selenium content,selenium speciation,and meat quality of grass *** diets were prepared,including a basal diet(BD)and three concentrations(0.1,0.3,and 0.9 mg/kg)of SeNP,selenite,and SeMet.A total of600 fish(250.79±1.57 g)were randomly assigned to 30 tanks(3 tanks/group).fish were fed the experimental diet three times daily for 60 *** this study,SeNP most significantly promoted the growth and antioxidant capacity of grass carp,with 0.3 mg/kg SeNP identified as the optimal additive ***,SeNP demonstrated equally excellent bioavailability as SeMet and significantly increased the content of SeMet in grass carp(Ctenopharyngodon idella)***,compared to SeMet and selenite,dietary SeNP could more significantly enhance the content of selenocysteine(SeCys2)and methylselenocysteine(MeSeCys)in grass carp muscle *** addition,we have demonstrated that SeCys2and MeSeCys promote apoptosis of cancer cells(HeLa)through the mitochondrial apoptotic pathway(involving Bax and Bcl-2).furthermore,as an additive,0.3 mg/kg SeNP significantly improved the flesh quality of grass carp by reducing crude fat and heavy metal content,as well as increasing the levels of eicosapentaenoic acid(EPA)and docosahexaenoic acid(DHA)and the ratio of n-3/n-6 polyunsaturated fatty acid(PUfA).In summary,SeNP is the most suitable additive for producing selenium-enriched fish.

关键词： Selenium nanoparticle Speciation analysis Grass carp Selenium additive

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Maslinic acid supplementation prevents di(2-ethylhexyl)phthalate-induced apoptosis via PRDX6 in peritubular myoid cells of Chinese forest musk deer

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Journal of Environmental Sciences 2024年第9期143卷 47-59页

作者： Heran Cao Zhenpeng Li Tianqi Jin Shuyang He Shujuan Liu Long Li Yang Wang Ye Gong Gang Wang fangxia Yang Wuzi Dong college of Animal Science and Technology Northwest A&F UniversityShaanxi 712100China college of forestry Northwest A&F UniversityShaanxi 712100China Biology Research Centre of Qin-Mountains Wildlife Northwest A&F UniversityShaanxi 712100China Shaanxi Qiyuan-Times Agri-Tech Development Co.Ltd. Shaanxi 725000China

Chinese forest musk deer(fMD),an endangered species,have exhibited low reproductive rates even in captivity due to stress *** revealed the presence of di(2-ethylhexyl)phthalate(DEHP),an environmental endocrine disruptor,in the serum and skin of captive *** fMDs with maslinic acid(MA)has been observed to alleviate the stress response and improve reproductive rates,although the precise molecular mechanisms remain ***,this study aims to investigate the molecular mechanisms underlying the alleviation of DEHP-induced oxidative stress and cell apoptosis in primary peritubular myoid cells(PMCs)through MA *** PMCs were isolated and exposed to DEHP in *** results demonstrated that DEHP significantly suppressed antioxidant levels and promoted cell apoptosis in primary ***,interfering with the expression of PRDX6 was found to induce excessive reactive oxygen species(ROS)production and cell apoptosis in primary *** with MA significantly upregulated the expression of PRDX6,thereby attenuating DEHP-induced oxidative stress and cell apoptosis in primary *** findings provide a theoretical foundation for mitigating stress levels and enhancing reproductive capacity of in captive fMDs.

关键词： Maslinic acid forest musk deer Di(2-ethylhexyl)phthalate Peritubular myoid cells PRDX6

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Spatially resolved transcriptomic profiling of placental development in dairy cow

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Zoological Research 2024年第3期45卷 586-600页

作者： Guang-Hui Tan Shi-Jie Liu Ming-Le Dou De-feng Zhao Ao Zhang Heng-Kuan Li fu-Nong Luo Tao Shi Hao-Ping Wang Jing-Yuan Lei Yong Zhang Yu Jiang Yi Zheng fei Wang Key Laboratory of Animal Genetics Breeding and Reproduction of Shaanxi ProvinceCollege of Animal Science and TechnologyNorthwest A&F UniversityYanglingShaanxi 712100China Key Laboratory of Livestock Biology Northwest A&F UniversityYanglingShaanxi 712100China Key Laboratory of Animal Biotechnology of the Ministry of Agriculture Northwest A&F UniversityYanglingShaanxi 712100China college of Information Engineering Northwest A&F UniversityYanglingShaanxi 712100China

The placenta plays a crucial role in successful mammalian *** animals possess a semi-invasive placenta characterized by a highly vascularized structure formed by maternal endometrial caruncles and fetal placental cotyledons,essential for full-term fetal *** cow placenta harbors at least two trophoblast cell populations:uninucleate(UNC)and binucleate(BNC)***,the limited capacity to elucidate the transcriptomic dynamics of the placental natural environment has resulted in a poor understanding of both the molecular and cellular interactions between trophoblast cells and niches,and the molecular mechanisms governing trophoblast differentiation and *** fill this knowledge gap,we employed Stereo-seq to map spatial gene expression patterns at near single-cell resolution in the cow placenta at 90 and 130 days of gestation,attaining high-resolution,spatially resolved gene expression *** on clustering and cell marker gene expression analyses,key transcription factors,including YBX1 and NPAS2,were shown to regulate the heterogeneity of trophoblast cell *** communication and trajectory analysis provided a framework for understanding cell-cell interactions and the differentiation of trophoblasts into BNCs in the placental *** analysis of cell trajectories identified a set of genes involved in regulation of trophoblast ***,spatial modules and co-variant genes that help shape specific tissue structures were ***,these findings provide foundational insights into important biological pathways critical to the placental development and function in cows.

关键词： Spatial transcriptomics Dairy cow Placenta Gestation

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