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Exploring the genetic characteristics of 93-11 and Nipponbare recombination inbred lines based on the Golden Gate SNP assay

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Science China(life sciences) 2016年第7期59卷 700-707页

作者： Renbo Yu Wei Yan Manzhong Liang Xiaojun Dai Haodong Chen Yunong Sun Xingwang Deng Xiangding Chen Hang He Liangbi Chen College of life sciences Hunan Normal University State Key Laboratory of Protein and Plant Gene Research Peking-Tsinghua Center for Life Sciences School of Advanced Agriculture Sciences and School of Life Sciences Peking University College of life sciences Capital Normal University

Understanding genetic characteristics in rice populations will facilitate exploring evolutionary mechanisms and gene cloning. Numerous molecular markers have been utilized in linkage map construction and quantitative trait locus （QTL） mappings. However, segregation-distorted markers were rarely considered, which prevented understanding genetic characteristics in many populations. In this study, we designed a 384-marker GoldenGate SNP array to genotype 283 recombination inbred lines （RILs） derived from 93-11 and Nipponbare Oryza sativa crosses. Using 294 markers that were highly polymorpbic between parents, a linkage map with a total genetic distance of 1,583.2 cM was constructed, including 231 segregation-distorted mark- ers. This linkage map was consistent with maps generated by other methods in previous studies. In total, 85 significant quanti- tative trait loci （QTLs） with phenotypic variation explained （PVE） values〉5% were identified. Among them, 34 QTLs were overlapped with reported genes/QTLs relevant to corresponding traits, and 17 QTLs were overlapped with reported sterili- ty-related genes/QTLs. Our study provides evidence that segregation-distorted markers can be used in linkage map construc- tion and QTL mapping. Moreover, genetic information resulting from this study will help us to understand recombination events and segregation distortion. Furthermore, this study will facilitate gene cloning and understanding mechanism of in- ter-subspecies hybrid sterility and correlations with important agronomic traits in rice.

关键词： GoldenGate SNP assay linkage map segregation-distorted markers QTLs

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Alleviation effects of Ce^(3+) on inhibition of photochemical activity caused by linolenic acid in spinach chloroplast

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Journal of Rare Earths 2008年第6期26卷 875-879页

作者：刘晓晴黄浩刘超马玲兰刘洁殷四涛洪法水 College of life sciences Suzhou University

Linolenic acid has great effects on the structure and function of chloroplast. The function of Ce^3＋ on the improvement of chloroplast photoreduction activity and oxygen evolution damaged by linolenic acid in spinach by in vitro investigation was studied. Results showed that adding Ce^3＋ to the linolenic acid treated chloroplast could greatly decrease the reduction linolenic acid exerted on the whole chain electron transport rate and the photoreduction activity of photosystem Ⅱ （PSII） and photosystem Ⅰ （PSI） as well as the oxygen evolution rate of chloroplast. It indicated that Ce^3＋ had the ability to relieve the inhibition of the photochemical reaction of chloroplast caused by linolenic acid to some extent.

关键词： Ce^3+ linolenic acid chloroplast photochemical action rare earths

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Chemical synthesis of crystalline proteins

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Science China Chemistry 2015年第12期58卷 1779-1781页

作者： Yichao Huang Lei Liu Tsinghua-Peking Center for life sciences

Proteins play pivotal roles in the biochemistry of living *** understanding of how proteins such as enzymes and transport receptors function has long been an important research subject from views of both academia and

关键词： Chemical synthesis of crystalline proteins

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Ten years of achievements in biological and medical sciences

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Science China(life sciences) 2017年第2期60卷 111-115页

作者： Mingyao Liu Tieliu Shi Shanghai Key Laboratory of Regulatory Biology Institute of Biomedical Sciences and School of Life Sciences East China Normal University Shanghai 200241 China

This special issue commemorates the 10th anniversary of Institute of Biomedical sciences （IBMS）, East China Nor- mal University （ECNU）. IBMS was established in 2007 by a group of oversea scientists from Harvard Medical School, Yale University, the Texas A＆M University, Baylor Col- lege of Medicine, the University of Texas MD Anderson Cancer Center, the University of California and the Scripps Research Institute. These scientists were brought together on the initiative of Dr. Mingyao Liu, who was then ap- pointed as the director of IBMS and dean of School of life sciences, ECNU. The institute is located on the new ECNU campus in Minhang district of Shanghai. IBMS has a mis- sion to promote and advance biological and medical scienc- es in all their forms through research, education, communi- cation and collaboration.

关键词：生物医学生物学华东师范大学加利福尼亚大学科学研究所 IBMS 生命科学学院德克萨斯

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Bioaccumulation, subcellular distribution and chemical forms of yttrium in rice seedling

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Journal of Rare Earths 2018年第3期36卷 331-336页

作者： Shengnan Zheng Caiyun Zhang Kailun Shi Jinxiao Wang Guanjun Sun Qiaochu Hu Fengyun Zhao Xue Wang School of life sciences Shandong University of Technology

Yttrium（Y） accumulation in soil is a serious environment problem in China. To understand the mechanisms involved in Y tolerance and detoxification in rice seedling, the bioaccumulation, subcellular distribution, and chemical forms of Y was investigated in the present study. The results show that the content of Y both in roots and shoots increases with the increasing concentration of Y, and a large amount of Y is stored in roots. Subcellular distribution of Y in rice indicates that the majority of Y is bound to cell wall. Meanwhile, various chemical forms of Y is absorbed by rice. The greatest amount of Y is extracted by 2% hydrate acetic acid（HAc） and 0.6 mol/L HCl（particularly 2% HAc）, which indicates that most of Y is combined with un-dissolved phosphate and oxalate（particularly phosphate）. Cluster analysis, based on Y abundance levels, reveals the relationship between Y distribution patterns and chemical forms. Our results imply that Y forming of precipitates with phosphate（and/or oxalate） and depositing in the cell wall may be a key strategy for Y detoxicity and tolerance in rice.

关键词： Subcelluar distribution Yttrium Detoxicity Tolerance Rice Rare earths

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A homing rescue gene drive with multiplexed gRNAs reaches high frequency in cage populations but generates functional resistance

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Journal of Genetics and Genomics 2024年第8期51卷 836-843页

作者： Shibo Hou Jingheng Chen Ruobing Feng Xuejiao Xu Nan Liang Jackson Champer Center for Bioinformatics Center for Life SciencesSchool of Life SciencesPeking UniversityBeijing 100871China

CRISPR homing gene drives have considerable potential for managing populations of medically and agriculturally significant *** operate by Cas9 cleavage followed by homology-directed repair,copying the drive allele to the wild-type chromosome and thus increasing in frequency and spreading throughout a ***,resistance alleles formed by end-joining repair pose a significant *** address this,we create a homing drive targeting the essential hairy gene in Drosophila *** resistance alleles are recessive lethal,while drive carriers have a recoded“rescue”version of *** drive inheritance rate is moderate,and multigenerational cage studies show drive spread to 96%–97%of the ***,the drive does not reach 100%due to the formation of functional resistance alleles despite using four *** alleles have a large deletion but likely utilize an alternate start ***,revised designs targeting more essential regions of a gene may be necessary to avoid such functional *** of the rescue element’s native 3'UTR with a homolog from another species increases drive inheritance by 13%–24%.This was possibly because of reduced homology between the rescue element and surrounding genomic DNA,which could also be an important design consideration for rescue gene drives.

关键词： Genedrive Homing drive Rescueelement Population modification Multiplexed gRNAs Resistance alleles Cage study

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The Ray Wu Society for life sciences

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Chinese Science Bulletin 2003年第8期48卷 839-840页

作者： MA Hong Department of Biology and The Huck Institute for life sciences, The Pennsylvania State University, 315 Wartik Laboratory, University Park, PA 16802, USA (e-mail: Hxm16@psu.edu) Department of Biology and The Huck Institute for life sciences The Pennsylvania State University USA

Focuses on the Ray Wu Society for life sciences (RWS), a non-profit professional society of Chinese-American and Chinese life scientists. Goal of the organization; Total membership; Elected officers of the organization.

关键词：生命科学中国美国学术交流科学研究

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Prevention of La^(3+) on DNA Damage Caused by Hg^(2+) from Fish Intestines

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Journal of Rare Earths 2007年第2期25卷 243-248页

作者：洪法水王玲刘超苏明玉黄浩陈亮 College of life sciences Library Suzhou University

The aim of this study was to investigate the effects of rare earth elements （REEs） in preventing Hg^2＋ pollution, using fish intestinal DNA in vitro and study the mechanism of the interactions between Hg^2＋ , La^3＋ , the mixture of La^3＋ and Hg^2＋ and DNA by spectroscopy. The interactions between Hg^2＋ , La^3＋ , the mixture of La^3＋ and Hg^2＋ and DNA from fish intestine in vitro was investigate by using absorption spectrum and fluorescence emission spectrum. Ultraviolet absorption spectra indicated that the addition of Hg^2＋ , La^3＋ , and the mixture of La^3＋ and Hg^2＋ to DNA generated obvious hypochromic effect. Meanwhile, the 205.2 nm peak of DNA blue and the 258.2 nm peak of DNA red shifted. The hypochromic effect and peak shift was caused by these ions in an order of Hg^2＋〉 Hg^2＋＋ La^3＋〉 La^3＋ . The fluorescence emission spectra showed that as the addition of Hg^2＋ , La^3＋ , and the mixture of La^3＋ and Hg^2＋ , the emission peak at about 416.2 nm of DNA did not obviously change, but the fluorescence intensity reduced gradually with the order in treatment was Hg^2＋〉 Hg^2＋〉 La^3＋〉 La^3＋ . Hg^2＋ , La^3＋ , and the mixture of La^3＋ and Hg^2＋ had 1.12, 0.58, and 0.81 binding sites to DNA, the fluorescence quenching of DNA caused by them all attributed to static quenching. The binding constants KA of binding sites were 3.82×10^4 and 4.22×10^2 L·mol^-1 ; 2.50×10^4 and 2.95×10^3 L·mol^-1 ; 3.05×10^4 and 1.00×10^3 L·mol^-1. The results showed that La^3＋ could relieve destruction caused by Hg^2＋ on the DNA structure.

关键词： Hg^2+ , La^3+ fish intestinal DNA spectral characterization binding constants rare earths

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Carbon Monoxide Alleviates Wheat Seed Germination Inhibition and Counteracts Lipid Peroxidation Mediated by Salinity

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Journal of Integrative Plant Biology 2006年第10期48卷 1168-1176页

作者： Sheng Xu Zhi-Sheng Sa Ze-Yu Cao Wei Xuan Ben-Kai Huang Teng-Fang Ling Qiong-Ying Hu Wen-Biao Shen College of life sciences Laboratory Centre of Life Sciences Nanjing Agricultural University Nanjing 210095 China

Recently In animals, endogenous carbon monoxide （CO）, like nitric oxlde, was implicated as another Important physiological messenger or bioactive molecule. However, little information is known about the physlologlcal roles of CO in the whole plant. In the present study, we report that different concentrations of the 130 donor hematin （0.1, 1.0 and 10.0 μmol/L） alleviated wheat （Tilticum aestivum L. Yangmai 158） seed germination Inhlbltlon caused by 250 mmol/L NaCI stress In a dose-dependent manner. These responses were also proved by the addltion of different gaseous CO aqueous solutions from 0.1% to 100.0% of saturation. Among these treatments, the effect of 1.0 μmol/L hematin and 1.0% saturation of CO aqueous solution were the most obvlous. Furthermore, compared with non-hematin treatment, the degradation of storage reserves In wheat seeds was also accelerated. Time-course analyses showed that application of hematln dose-dependently Increased the activities of superoxide dismutase, catalase, ascorbate peroxidase, and guaiacol peroxidase activities, thus decreasing the lipid peroxidation In germinating wheat seed subjected to salt stress. Meanwhile, the responses of hematin were specific for CO because the addition of the CO scavenger hemoglobln （0.2 g/L） blocked the various actions of 1.0 μmol/L hematin. Taken together, the results of the present study demonstrate that CO, at a low concentration, is able to attenuate the seed germlnation Inhibition produced by salinity stress and counteract the lipid peroxidation in germinating wheat seeds.

关键词： carbon monoxide lipid peroxidation salt stress seed germination storage reserve degradation wheat

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The size matters： regulation of lipid storage by lipid droplet dynamics

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Science China(life sciences) 2017年第1期60卷 46-56页

作者： Jinhai Yu Peng Li Tsinghua-Peking Center for life sciences School of Life Sciences Tsinghua University Beijing 100084 China

Adequate energy storage is essential for sustaining healthy life. Lipid droplet （LD） is the subcellular organelle that stores energy in the form of neutral lipids and releases fatty acids under energy deficient conditions. Energy storage capacity of LDs is primarily dependent on the sizes of LDs. Enlargement and growth of LDs is controlled by two molecular pathways： neutral lipid synthesis and atypical LD fusion. Shrinkage of LDs is mediated by the degradation of neutral lipids under energy demanding conditions and is controlled by neutral cytosolic lipases and lysosomal acidic lipases. In this review, we summarize recent progress regarding the regulatory pathways and molecular mechanisms that control the sizes and the energy storage capacity of LDs.

关键词： lipid storage lipid droplet TAG synthesis atypical LD fusion lipolysis

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