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Yeast-Controlled Double-Shelled CacO_(3)/CaF_(2)Hollow Nanospheres with Hierarchically Porous for Sustained pH-Sensitive Drug Release

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Chinese Journal of Chemistry 2024年第15期42卷 1713-1720页

作者： Xinhe Liu Yi Chang Guanglei Ma Tingting Liu Penghui Song Heng Yu Xueqing Ren Yuming Guo Xiaoming Ma School of Chemistry and Chemical Engineering Henan Normal UniversityXinxiangHenan 453007China collaborative innovation center of henan province for Green Manufacturing of Fine Chemicals Key Laboratory of Green Chemical Media and ReactionsMinistryof EducationHenan Normal UniversityXinxiangHenan 453007China

Hierarchically porous materials(HP materials)are believed one of the most hopeful matrix materials because of their distinctive multimodal pore structures and tremendous application potentials in the field of ***,green and facile synthesis of hierarchically porous nanomaterials with beneficial water dispersibility and biocompatibility is still a great ***,a novel biomimetic strategy is proposed to prepare the cell-tailored double-shelled HPCaCO_(3)/CaF_(2) hollow nanospheres under the mediation of yeast *** biomolecules derived from the secretion of yeast cells are used as conditioning and stabilizing agents to control the biosynthesis of the HPCaCO_(3)/CaF_(2) materials,which exhibit excellent water dispersibility and favorable *** double-shelled CaCO_(3)/CaF_(2) nanospheres hold hierarchically porous structure and have abundant pore channel and large specific surface area,showing high drug-loading and a prolonged drug sustainable release profile by the pore-by-pore diffusion pattern of the hierarchical ***,the HPCaCO_(3) with pH-sensitivity could controllably release drug doxorubicin hydrochloride(DOX)at the acidic tumor *** in vitro and in vivo results demonstrate that HPCaCO_(3)/CaF_(2) has the sustainable pH-sensitive drug release property,showing an enhanced therapeutic ***,this study provides a biomimetic strategy to synthesize the hierarchically porous double-shelled hollow nanomaterials for applying in sustainable drug delivery system.

关键词： Biomimetic synthesis Hierarchically porous Sustained pH-sensitive drug release Antitumor agents Drug delivery Calcium Cancer therapy/Hollow structures

A novel method for the green utilization of waste fried oil

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Particuology 2024年第1期84卷 1-11页

作者： Gan Cheng Mengni Zhang Yang Lu Yanhui Zhang Bin Lin Ee Von Lau College of Chemistry and Chemical Engineering Henan Polytechnic UniversityJiaozuo454003China henan Key Laboratory for Green and Efficient Mining&Comprehensive Utilization of Mineral Resources Henan Polytechnic UniversityJiaozuo454003China collaborative innovation center of coal Work safety and Clean High Efficiency Utilization Henan Polytechnic UniversityJiaozuo454003China School of Engineering Monash University MalaysiaSubang JayaSelangor47500Malaysia

Waste fried oil was studied to prepare three different types of detergent such as soap,liquid soap and soap powder via saponification *** preparation conditions of soap base were optimized by orthogonal *** specific preparation processes included waste fried oil treatment,orange peel extract preparation,saponification,demoulding and *** showed that the optimal con-ditions for saponification were as follows:ratio of pure waste fried oil to coconut oil=6∶4,alkali liquor(NaOH)mass fraction 30%,saponification temperature 70℃,orange peel extract concentration 15%.The mature soap was then used to make liquid soap and soap powder by surfactant(sodium dodecylbenzene sulfonate,coconut diethanol amide)addition,followed by *** prepared detergent conforms to the production standard of strong decontamination ability,possesses stable performance,is gentle to skin and non-toxic.

关键词： Waste fried oil Detergent Saponification Decolorization

The effect of glutathione on glucosinolate biosynthesis through the sulfur assimilation pathway in pakchoi associated with the growth conditions

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Horticultural Plant Journal 2024年第2期10卷 473-487页

作者： Biao Zhu Zhile Liang Dan Wang Chaochao He Zhujun Zhu Jing Yang Key Laboratory of Quality and safety Control for Subtropical Fruit and Vegetable Ministry of Agriculture and Rural AffairsCollaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang ProvinceCollege of Horticulture ScienceZhejiang A&F UniversityHangzhouZhejiang 311300China

Glucosinolates(GSLs) are a group of nitrogen-and sulfur-containing secondary metabolites, synthesized primarily in members of the Brassicaceae family, that play an important role in food flavor, plant antimicrobial activity, resistance to insect attack, stress tolerance, and human anti-cancer effects. As a sulfur-containing compound, glutathione has a strong connection with GSLs biosynthesis as a sulfur donor or redox system, and exists in reduced(glutathione;GSH) and oxidized(glutathione disulfide;GSSG) forms. However, the mechanism of GSH regulating GSLs biosynthesis remainds unclear. Hence, the exogenous therapy to pakchoi under normal growth condition and sulfur deficiency condition were conducted in this work to explore the relevant mechanism. The results showed that exogenous application of buthionine sulfoximine, an inhibitor of GSH synthesis, decreased the transcript levels of GSLs synthesis-related genes and transcription factors, as well as sulfur assimilation-related genes under the normal growth condition. Application of exogenous GSH inhibited the expression of GSLs synthesis-and sulfur assimilation-related genes under the normal condition, while the GSLs biosynthesis and the sulfur assimilation pathway were activated by exogenous application of GSH when the content of GSH in vivo of plants decreased owing to sulfur deficiency. Moreover,exogenous application of GSSG increased the transcript levels of GSLs synthesis-and sulfur assimilation-related genes under the normal growth condition and under sulfur deficiency. The present work provides new insights into the molecular mechanisms of GSLs biosynthesis underlying glutathione regulation.

关键词： Pakchoi Glucosinolates Reduced glutathione Oxidized glutathione Sulfur assimilation

Detection of the farmland plow areas using RGB-D images with an improved YOLOv5 model

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International Journal of Agricultural and Biological Engineering 2024年第3期17卷 156-165页

作者： Jiangtao Ji Zhihao Han Kaixuan Zhao Qianwen Li Shucan Du College of Agricultural Equipment Engineering Henan University of Science and TechnologyLuoyang 471003HenanChina Science&Technology innovation center for Completed Set Equipment Longmen LaboratoryLuoyang 471023HenanChina collaborative innovation center of Machinery Equipment Advanced Manufacturing of henan province Luoyang 471003HenanChina School of Art and Design Henan University of Science and TechnologyLuoyang 471003HenanChina

Recognition of the boundaries of farmland plow areas has an important guiding role in the operation of intelligent agricultural *** precisely recognize these boundaries,a detection method for unmanned tractor plow areas based on RGB-Depth(RGB-D)cameras was proposed,and the feasibility of the detection method was *** method applied advanced computer vision technology to the field of agricultural *** and improving the YOLOv5-seg object segmentation algorithm,first,the Convolutional Block Attention Module(CBAM)was integrated into Concentrated-Comprehensive Convolution Block(C3)to form C3CBAM,thereby enhancing the ability of the network to extract features from plow *** GhostConv module was also utilized to reduce parameter and computational ***,using the depth image information provided by the RGB-D camera combined with the results recognized by the YOLOv5-seg model,the mask image was processed to extract contour boundaries,align the contours with the depth map,and obtain the boundary distance information of the plowed ***,based on farmland information,the calculated average boundary distance was corrected,further improving the accuracy of the distance *** experiment results showed that the YOLOv5-seg object segmentation algorithm achieved a recognition accuracy of 99%for plowed areas and that the ranging accuracy improved with decreasing detection *** ranging error at 5.5 m was approximately 0.056 m,and the average detection time per frame is 29 ms,which can meet the real-time operational *** results of this study can provide precise guarantees for the autonomous operation of unmanned plowing units.

关键词： plow areas RGB-D camera YOLO object segmentation contour boundary average distance

The cytosolic isoform of triosephosphate isomerase,ZmTPI4,is required for kernel development and starch synthesis in maize(Zea mays L.)

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The Crop Journal 2024年第2期12卷 401-410页

作者： Wenyu Li Han Wang Qiuyue Xu Long Zhang Yan Wang Yongbiao Yu Xiangkun Guo Zhiwei Zhang Yongbin Dong Yuling Li State Key Laboratory of Wheat and Maize Crop Science Collaborative Innovation Center of Henan Grain CropsCollege of AgronomyHenan Agricultural UniversityLongzi Lake CampusZhengzhou 450046HenanChina

Triosephosphate isomerase(TPI)is an enzyme that functions in plant energy production,accumulation,and *** understand its function in maize,we characterized a maize TPI mutant,*** comparison to the wild type,zmtpi4 mutants showed altered ear development,reduced kernel weight and starch content,modified starch granule morphology,and altered amylose and amylopectin ***,ATP,and pyruvate contents were reduced,indicating ZmTPI4 was involved in *** subcellular localization confirmed ZmTPI4 as a cytosolic rather than a plastid isoform of TPI,the zmtpi4 mutant showed reduced leaf size and chlorophyll *** of ZmTPI4 in Arabidopsis led to enlarged leaves and increased seed weight,suggesting a positive regulatory role of ZmTPI4 in kernel weight and starch *** conclude that ZmTPI4 functions in maize kernel development,starch synthesis,glycolysis,and photosynthesis.

关键词： Maize Kernel Starch Weight Photosynthesis

Method for the fruit tree recognition and navigation in complex environment of an agricultural robot

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International Journal of Agricultural and Biological Engineering 2024年第2期17卷 221-229页

作者： Xiaolin Xie Yuchao Li Lijun Zhao Xin Jin Shengsheng Wang Xiaobing Han College of Agricultural Equipment Engineering Henan University of Science and TechnologyLuoyang 471003HenanChina collaborative innovation center of Machinery Equipment Advanced Manufacturing of henan province Luoyang 471003HenanChina Longmen Laboratory Luoyang 471003HenanChina College of Intelligent and Manufacturing Engineering Chongqing University of Arts and SciencesChongqing 402160China

To realize the visual navigation of agricultural robots in the complex environment of orchards,this study proposed a method for fruit tree recognition and navigation based on *** YOLOv5s model was selected and trained to identify the trunks of the left and right rows of fruit trees;the quadratic curve was fitted to the bottom center of the fruit tree recognition box,and the identified fruit trees were divided into left and right columns by using the extreme value point of the quadratic curve to obtain the left and right rows of fruit trees;the straight-line equation of the left and right fruit tree rows was further solved,the median line of the two straight lines was taken as the expected navigation path of the robot,and the path tracing navigation experiment was carried out by using the improved LQR control *** experimental results show that under the guidance of the machine vision system and guided by the improved LQR control algorithm,the lateral error and heading error can converge quickly to the desired navigation path in the four initial states of[0 m,−0.34 rad],[0.10 m,0.34 rad],[0.15 m,0 rad]and[0.20 m,−0.34 rad].When the initial speed was 0.5 m/s,the average lateral error was 0.059 m and the average heading error was 0.2787 rad for the navigation trials in the four different initial *** average driving was 5.3 m into the steady state,the average value of steady state lateral error was 0.0102 m,the average value of steady state heading error was 0.0253 rad,and the average relative error of the robot driving along the desired navigation path was 4.6%.The results indicate that the navigation algorithm proposed in this study has good robustness,meets the operational requirements of robot autonomous navigation in orchard environment,and improves the reliability of robot driving in orchard.

关键词： fruit tree recognition visual navigation YOLOv5 complex environments orchards

Patterns, processes, and differentiation mechanisms of China's transition from old to new economic drivers

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Journal of Geographical Sciences 2024年第9期34卷 1697-1718页

作者： YAN Mingtao ZHAO Jianji YAN Mingyue Key Research Institute of Yellow River Civilization and Sustainable Development Henan UniversityKaifeng 475001HenanChina collaborative innovation center on Yellow River Civilization Jointly Built by henan province and Ministry of Education Henan UniversityKaifeng 475001HenanChina College of Cultural and Tourism Henan UniversityKaifeng 475001HenanChina

Transition from old to new economic drivers(TONED) is of great significance for optimizing economic structures. Using a combination of methods including entropy weight technique for order preference by similarity to ideal solution, geospatial analysis, and optimal parameter geodetector, this study investigates the patterns and mechanisms of China's TONED in the prefecture-level cities from 2011 to 2020. The findings reveal that(1) the TONED exhibited a spatial distribution characterized by higher levels in the east and lower levels in the west, along with a pyramidal hierarchy of levels. The distribution of hot and cold spots roughly followed the Heihe-Yulin line.(2) The informatization levels, openness to the outside world, and urbanization played a dominant role in the TONED. The types of dual-factor interactions exhibited both dual-factor enhancement and non-linear enhancement effects.(3) The eastern and central regions were more likely to promote the TONED through enhanced informatization levels, whereas the western region relied more on increasing openness. This study finds that the TONED results from the combined effects of three geographical natures, each playing a distinct role. The recommendations provided can serve as a reference for China and other developing countries to implement the TONED in the context of deglobalization.

关键词： transition from old to new economic drivers pattern evolution differentiation mechanism geographical nature China

The BTB/TAZ domain-containing protein CmBT1-mediated CmANR1 ubiquitination negatively regulates root development in chrysanthemum

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Journal of Integrative Plant Biology 2024年第2期66卷 285-299页

作者： Lian-Da Du Zhang-Ji Guan Yan-Hong Liu Hui-Dong Zhu Quan Sun Da-Gang Hu Cui-Hui Sun Shandong collaborative innovation center of Fruit&Vegetable Quality and Efficient production College of Horticulture Science and EngineeringShandong Agricultural UniversityTaian 271018China

Roots are fundamental for plants to adapt to variable environmental *** development of a robust root system is orchestrated by numerous genetic determinants and,among them,the MADS-box gene ANR1 has garnered substantial *** research has demonstrated that,in chrysanthemum,CmANR1positively regulates root system ***,the upstream regulators involved in the CmANR1-mediated regulation of root development remain *** this study,we successfully identified bric-a-brac,tramtrack and broad(BTB)and transcription adapter putative zinc finger(TAZ)domain protein CmBT1 as the interacting partner of CmANR1 through a yeasttwo-hybrid(Y2H)screening ***,we validated this physical interaction through bimolecular fluorescence complementation and pull-down *** assays revealed that CmBT1 exerted a negative influence on root development in *** both in vitro and in vivo assays,it was evident that CmBT1mediated the ubiquitination of CmANR1 through the ubiquitin/26S proteasome *** ubiquitination subsequently led to the degradation of the CmANR1 protein and a reduction in the transcription of CmANR1-targeted gene CmPIN2,which was crucial for root development in *** analysis suggested that CmBT1 modulated root development,at least in part,by regulating the level of CmANR1 ***,these findings shed new light on the regulatory role of CmBT1 in degrading CmANR1 through ubiquitination,thereby repressing the expression of its targeted gene and inhibiting root development in chrysanthemum.

关键词： chrysanthemum MADS-box transcription factor root development

The intrinsic developmental age signal defines an age-dependent climbing behavior in cucumber

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Horticultural Plant Journal 2024年第3期10卷 797-808页

作者： Zezhou Hong Xinrui Wang Zipei Fan Jiahao Wang Aiyi Yang Guochao Yan Yong He Huasen Wang Zhujun Zhu Yunmin Xu College of Horticulture Science Zhejiang Agriculture and Forestry UniversityHangzhouZhejiang 311300China Key Laboratory of Quality and safety Control for Subtropical Fruit and Vegetable Ministry of Agriculture and Rural AffairsHangzhouZhejiang 311300China collaborative innovation center for Efficient and Green production of Agriculture in Mountainous Areas of Zhejiang province HangzhouZhejiang 311300China

The tendril is a climbing organ in cucurbits and functions in physical support and to avoid shading by neighboring ***,how cucurbits produce tendrils to obtain climbing ability is largely *** this study,tendril phenotypes were investigated during different developmental *** results revealed that tendril growth exhibited an age-dependent pattern in *** growth was inhibited,and the tendril was formed as a short tendril[nonfunctional tendril(nonF-tendril),approximately 0.1 cm]during the seedling *** contrast,enhanced cell proliferation and cell expansion led to rapid elongation of the tendril during the climbing stage,and the tendril formed as a functional tendril(F-tendril,approximately 30 cm)to obtain climbing ***-qPCR detection showed that age-dependent tendril growth correlated negatively with the abundance of the conserved age regulator *** induced CsmiR156 to inhibit CsSPLs,and F-tendril formation and climbing ability were delayed in defoliated cucumbers,which confirmed the role of CsmiR156 in regulating tendril growth in ***,exogenous gibberellin(GA)treatment showed that GA positively regulated tendril growth,and RT-qPCR detection showed that the GA bio-synthetic genes and metabolic genes were affected by age pathway,suggesting that the age pathway depended on GA bio-synthetic and metabolic pathway to regulate cell expansion to determine tendril *** summary,our work reveals that change in tendril type is an important marker of phase transition in cucumber,and tendril growth is regulated by an intrinsic developmental age signal,ensuring that the cucumber obtains climbing ability at a suitable age.

关键词： Cucumber Tendril Age CsmiR156 CsSPL Climbing ability