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Preparation and Characterization of Nano-ZnO Precursor for the Antibacterial Activity against Thielaviopsis paradoxa

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Plant Diseases and Pests 2022年第6期13卷 4-5,13页

作者： Yenan WANG Dejie YANG coconut research institute of chinese academy of tropical agricultural sciences Hainan Innovation Center of Academician TeamWenchang 571339China

[Objectives]The paper was to explore a new method for preparing nano ZnO particles.[Methods]A mixture of 2,6-di(2′,4′-dicarboxylphenyl)pyridine(0.05 mM),Zn(NO_(3))2·6H_(2)O(0.1 mM)were added to the solution of DMA(3 mL)and H_(2)O(3 mL),and heated at 90℃for 12 *** PXRD,TGA and fluorescence properties were characterized.[Results]Transparent block crystals of the[Zn_(2)(2,6-ddpp)(DMA)_(3)(H_(2)O)·H_(2)O]n were obtained(yield 85.4%,based on Zn).The Zn-MOF framework would collapse to form ZnO particles when the temperature exceeded 350℃.[Conclusions]A new Zn(II)-MOF is successfully constructed,and the compound exhibits monoclinic P21/c space *** results will provide a theoretical basis for the preparation of nano ZnO.

关键词： Nano ZnO Zn(II)-MOF P21/c space group

Molecular identification of a 16Sr Ⅱ-Ⅴ subgroup phytoplasma associated with Tephrosia purpurea Witches’-broom disease in hainan island of China

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Molecular identification of a 16Sr Ⅱ-Ⅴ subgroup phytoplasm...

中国植物病理学会2021年学术年会

作者： YU Shaoshuai ZHAO Ruiling LIN Mingxing WU Yuan SONG Weiwei YAN Wei coconut research institute of chinese academy of tropical agricultural sciences Hainan Innovation Center of Academician Team hainan Duoyuan Arecanut Industry Development Company Limited

Tephrosia purpurea is a excellent insecticidal plant belonging to the family of Leguminosae distributed in *** symptoms of the plant including witches’ broom,internode shortening,leaf chlorosis and leaflet were found in Ledong county of hainan province,a tropical island in China.16 S rRNA and secA gene sequence fragments of phytoplasma were detected through PCR amplification using primers R16 mF2/R16 mR1 and secAforl/*** two gene sequence fragments of phytoplasma were obtained in the DNA from six symptomatic plant samples whereas not in the DNA from six symptomless plant *** DNA of Tephrosia purpurea samples were extracted by CTAB method.16 S rRNA and secA gene sequence fragments of phytoplasma were detected through PCR amplification using primers R16 mF2/R16 mR1 and secAforl/*** two gene sequence fragments of phytoplasma were obtained in the DNA from six symptomatic plant samples whereas not in the DNA from six symptomless plant *** two gene sequence fragments of the DNA obtained from the disease plant samples were all identical,with the length of 1335 bp 16 S rRNA and 729 bp secA gene sequence fragments,putatively encoding 242（secA） amino acids *** phytoplasma strain was named as Tephrosia purpurea witches’-broom（TpWB） phytoplasma,a 16 SrⅡ-Ⅴ subgroup strain,which was analyzed by online analysis of *** our knowledge,this was the first time that 16 SrⅡ-Ⅴ subgroup phytoplasma associated with TpWB disease was identification in *** analysis based on the 16 S rRNA and secA gene sequence fragments indicated that TpWB-hnld phytoplasma was more closely related to the phytoplasma strains belonging to 16 SrⅡ group.

关键词： phytoplasma Tephrosia purpurea witches’-broom disease 16Sr Ⅱ-Ⅴ subgroup molecular identification

Occurrence of 16Sr Ⅱ group related phytoplasma associated with Emilia sonchifolia Witches’-broom disease in hainan island of China

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Occurrence of 16Sr Ⅱ group related phytoplasma associated w...

中国植物病理学会2021年学术年会

作者： YU Shaoshuai ZHAO Ruiling LIN Mingxing WU Yuan CHEN Shugui YU Fengyu SONG Weiwei ZHU Hui coconut research institute of chinese academy of tropical agricultural sciences Hainan Innovation Center of Academician Team hainan Duoyuan Arecanut Industry Development Company Limited

Emilia sonchifolia is a kind of medical and economical plants belonging to the family of Asteraceae,mainly used as a traditional chinese *** October to November 2020,the plants showing abnormal symptoms including witches’-broom,internode shortening,leaf chlorosis and leaflet were found in hainan province,a tropical island of *** total DNA of the plant samples were extracted using 0.10 g fresh plant leaves using CTAB *** reactions were performed using primers R16 mF2/R16 mR1 and secAforl/secArev3 specific for phytoplasma 16 S rRNA and secA gene *** target productions of the two gene fragments of phytoplasma were detected in the DNA from three symptomatic plant samples whereas not in the DNA from the symptomless plant *** two gene fragments of the DNA extracted from the symptomatic plant samples were all identical,with the length of 1324 bp 16 S rRNA and 760 bp secA gene sequence fragments,putatively encoding 253（secA） amino acids *** phytoplasma strain was named as Emilia sonchifolia witches’-broom（EsWB） phytoplasma,EsWB-hnda *** our knowledge,this was the first report that Emilia sonchifolia witches’-broom disease was caused by the phytoplasma belonging to16 SrⅡ-Ⅴ subgroup in hainan island of China,with close relationship to 16 SrⅡ peanut witches’-broom group phytoplasma strains infecting the plants like peanut,Desmodium ovalifolium and cleome from the same island of China and cassava from Viet Nam.

关键词： phytoplasma Emilia sonchifolia witches’-broom disease 16SrⅡ group molecular detection

Waltheria indica represents a new host of 16SrⅠ-B subgroup phytoplasma associated with virescence symptoms in China

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Waltheria indica represents a new host of 16SrⅠ-B subgroup ...

中国植物病理学会2021年学术年会

Waltheria indica is a kind of plants belonging to Sterculiaceae mainly used as a traditional chinese *** September to November 2020,the plants showing abnormal symptoms including floral virescence,leaf chlorosis and leaflet were found in Dingan county of hainan province,China and severely impacted their growth resulting in financial loss and ecological *** total DNA of symptom or symptomless Waltheria indica samples were extracted using 0.10 g fresh plant tissues using CTAB *** reactions were performed using primers R16 mF2/R16 mR1 and AYgroelF/AYgroelR specific for phytoplasma 16 S rRNA and groEL gene *** target productions of the two gene fragments of phytoplasma were detected in the DNA from four symptomatic plant samples whereas not in the DNA from the symptomless plant *** two gene fragments of the DNA extracted from the symptom plant samples were all identical,with the length of 1340 bp 16 S rRNA and1312 bp groEL gene sequence fragments,putatively encoding 437(groEL) amino acids *** phytoplasma strain was named as Waltheria indica virescence(WiV) phytoplasma,WiVnda *** our knowledge,this is the first time that Waltheria indica virescence disease inducted by 16 SrIB subgroup phytoplasma strain was reported in *** analysis showed that WiV-hnda was closely related to the phytoplasma strains causing Onion yellows in Japan,Periwinkle virescence and Chinaberry witches’-broom disease in China.

关键词： phytoplasma Waltheria indica virescence disease 16SrⅠ-B subgroup molecular detection

Detection of phytoplasma associated with arecanut（Areca cathecu L.）yellow leaf in hainan island of China by LAMP method

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Detection of phytoplasma associated with arecanut（Areca cat...

中国植物病理学会2021年学术年会

作者： YU Shaoshuai CHE Haiyan WANG Shengjie LIN Caili LIN Mingxing SONG Weiwei TANG Qinghua YAN Wei QIN Weiquan coconut research institute of chinese academy of tropical agricultural sciences Hainan Innovation Center of Academician Team Environment and Plant Protection institute Chinese Academy of Tropical Agricultural Sciences research institute of tropical Forestry Chinese Academy of Forestry research institute of Forest Ecology Environment and ProtectionChinese Academy of Forestry

Arecanut（Areca cathecu L.） yellow disease is a destructive disease caused by the phytoplasma,a serious threat to the healthy development of arecanut industry in hainan Province for a long *** 16 S rRNA gene sequence was employed as the target to establish a rapid and efficient detection system for arecanut yellow leaf phytoplasma belonging to 16 SrI group in China by loop mediated isothermal amplification（LAMP） in the study,with testing the specificity,sensitivity and stability of the detection *** technology aiming to 16 SrI group arecanut yellow leaf phytoplasma in China was used to detect the samples of the arecanut yellow leaf disease from different areas of hainan *** results showed that two sets of LAMP detection primers,16 SrDNA-2 and 16 SrDNA-3 were obtained for the 16 SrI group arecanut yellow leaf phytoplasma in China based on the 16 S rRNA gene sequence,both with good specificity and stability;The lowest detection limit of sensitivity of the two sets of LAMP detection systems was the same,200 ag/μL;Phytoplasmas were all detected in arecanut yellow leaf disease samples from Baoting,Tunchang,Wanning in hainan province of China by using the two sets of LAMP primers 16 SrDNA-2 and 16 SrDNA-3,and no phytoplasma was detected in the negative *** detection technology is fast,efficient,easily operate and the results are visual,suitable for application and research in the rapid diagnosis of arecanut yellow disease in the field,the detection of seedlings with pathogen and the breeding of resistant arecanut *** is of great significance for the detection and identification of the arecanut yellow disease pathogen,the spread and scientific control of the disease.

关键词： phytoplasma arecanut yellow leaf disease LAMP 16S rDNA Rapid detection

Crystal Structure of Poly[(2,6-di(2’,4’-dicarboxylphenyl)pyridine-κ4 O:O′:O″:O′′′)-(μ2-1,4-bis(1-imidazolyl)benzene-κ2N:N’)cobalt(II)],C_(39)H_(24)Co_(2)N_(7)O_(8)

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Plant Diseases and Pests 2021年第1期12卷 38-43页

作者： Wang Yenan Liu Bo Niu Xiaoqing coconut research institute of chinese academy of tropical agricultural sciences Hainan Innovation Center of Academician TeamWenchang 571339China

[Objective]The paper was to synthesize a new structure of metal organic framework,and to provide new materials for plant pathogens testing.[Method]A mixture of 2,6-di(2’,4’-di-carboxylphenyl)pyridine,1,4-bis(1-imidazolyl)benzene,Co(NO_(3))2·6H_(2)O were added to the solution of DMF:H2O=3:3 and heated at 433K for 4 d.[Result]Red crystals of the title compound were obtained(yield 76.3%,based on Co).[Conclusion]The crystal data were obtained as follows:C_(39)H_(24)Co_(2)N_(7)O_(8),monoclinic,P21/c,a=16.222(7)A,b=12.653(5)A,c=17.456(7)A,β=102.386(6)°,V=3500(2)A_(3),Z=4,Rgt(F)=0.0550,wRref(F2)=0.1509,T=293(2)K.

关键词： Poly[(2,6-di(2’,4’-dicarboxylphenyl)pyridine Cobalt(II)

Syntheses,Structures of A Cobalt(Ⅱ) Metal-organic Framework Based on 2,6-di(2',4'-dicarboxylphenyl) pyridine and 4,4'-bis(imidazol-1-ylmethyl) biphenyl-ligands

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Plant Diseases and Pests 2022年第5期13卷 4-9页

作者： Yenan WANG Xiaoqing NIU Bo LIU coconut research institute of chinese academy of tropical agricultural sciences Hainan Innovation Center of Academician TeamWenchang 571339China

[Objectives]The paper was to synthesize and describe a new 3D structure of a mew metal organic framework,and to provide new materials for the study of interdisciplinary.[Methods]A mixture of H_(4)L=2,6-di(2,4-dicarboxylphenyl)pyridine,bimb=4,4-bis(imidazole-1-ylmethyl)biphenyl,Co(NO_(3))_(2)·6H_(2)O were added to the solution of DMF∶H_(2)O=4∶2 and heated at 433 K for 4 d.[Results]Purple block crystals of the Co-compound were obtained(yield 83.6%,based on Co).[Conclusions]The crystal data are obtained as follows:C_(51)H_(36)Co_(2)N_(7)O_(9),Triclinic,P-1,a=9.916(8),b=15.926(14),c=15.945(13),α=113.913(13)°,β=93.929(9)°,γ=95.033(3)°,V=2278(3)A^(3),Z=2,R=0.0803(6446),wR_(2)=0.2106(10370),T=296 K.

关键词： Cobalt(Ⅱ) Metal-organic framework Crystal Syntheses Structure

Genetic diversity, population structure, and genome-wide association analysis of ginkgo cultivars

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Horticulture research 2023年第8期10卷 231-240页

作者： Yaping Hu Zhaoyan Yu Xiaoge Gao Ganping Liu Yun Zhang Petr Smarda Qirong Guo Co-innovation center for Sustainable Forestry in Southern China Nanjing Forestry UniversityNanjing 210037China coconut research institute of chinese academy of tropical agricultural Science WenchangHainan 571339China institute of Grassland Flowersand EcologyBeijing Academy of Agriculture and Forestry SciencesBeijing 100097China Department of Botany and Zoology Faculty of ScienceMasaryk UniversityKoltlárská2Brno 61137Czech Republic

Ginkgo biloba is an economically valuable tree *** species has nearly become extinct during the Quaternary,which has likely resulted in reduction of its genetic *** genetic variability is now conserved in few natural populations in China and a number of cultivars that are,however,derived from a few ancient trees,helping the species survive in China through medieval *** the recent interest in ginkgo,however,detailed knowledge of its genetic diversity,conserved in cultivated trees and cultivars,has remained *** limits efficient conservation of its diversity as well as efficient use of the existing germplasm *** we performed genotyping-by-sequencing(GBS)on 102 cultivated germplasms of ginkgo collected to explore their genetic structure,kinship,and inbreeding *** the first time in ginkgo,a genome-wide association analysis study(GWAS)was used to attempt gene mapping of seed *** results showed that most of the germplasms did not show any obvious genetic *** size of the ginkgo germplasm population expanded significantly around 1500 years ago during the Sui and Tang *** of seed cultivars based on a phylogenetic perspective does not support the current classification criteria based on ***-four candidate genes were localized after performing GWAS on the seed ***,this study reveals the genetic basis of ginkgo seed traits and provides insights into its cultivation *** findings will facilitate the conservation and utilization of the domesticated germplasms of this living fossil plant.

关键词： cultivar likely expanded

research Advances in Stem Bleeding Disease of Cocos nucifera L.

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Plant Diseases and Pests 2020年第5期11卷 41-45页

作者： Niu Xiaoqing Yu Fengyu Yang Dejie Zhu Hui coconut research institute of chinese academy of tropical agricultural sciences Wenchang 571339China China tropical Oil Crop research center Wenchang 571339China hainan innovation center of academician team Wenchang 571339China

Stem bleeding disease of coconut(Cocos nucifera L.)is an important disease in main coconut producing areas in *** article reviews taxonomic status and biological characteristics of Ceratocystis paradoxa,as well as the occurrence regularity,pathogenic factors,propagation path and integrated control methods of stem bleeding disease of *** main problems in the study of stem bleeding disease are summarized,and the future research directions are prospected.

关键词： Stem bleeding disease of coconut Occurrence regularity Integrated control Pathogenic factor