检索结果-南通市图书馆

Microvesicles derived from hypoxia/reoxYgenation-treated human umbilical vein endothellal cells impair relaxation of rat thoracic aortic rings

引用

中国应用生理学杂志 2014年第6期30卷 560-566页

作者： Shao-xun WANG Qi ZHANG Man SHANG Su WEI Miao LIU Yi-lu WANG Meng-xiao ZHANG Yan-na WU Ming-lin LIU Jun-qiu SONG Yan-xia LIU Department of pharmacology School of Basic Medical SciencesTianjin Medical University section of Endocrinology Department of MedicineTemple University School of Medicine Department of Dermatology Perelman School of MedicineUniversity of Pennsylvania

Objective To investigate the effects of microvesicles(MVs) derived from hypoxia/reoxygenation(H/R)-treated human umbilical vein endothelial cells(HUVECs) on endothelium-dependent relaxation of rat thoracic aortic *** H/R injury model was established to induce HUVECs to release H/R-EMVs.H/R-EMVs from HUVECs were isolated by ultracentrifugation from the conditioned culture medium.H/R-EMVs were characterized using 1 urn latex beads and anti-PE-CD144 by flow *** aortic rings of rats were incubated with 2.5,5,10,20 μg/ml H/R-EMVs derived from H/R-treated HUVECs for 4 hours,and their endothelium-dependent relaxation in response to acetylcholine(ACh) or endothelium-independent relaxation in response to sodium nitroprusside(SNP) was recorded in *** nitric oxide(NO) production of ACh-treated thoracic aortic rings of rats was measured using Griess *** expression of endothelial NO synthase(eNOS) and phosphorylated eNOS(p-eNOS,Ser-1177) in the thoracic aortic rings of rats was detected by Western ***,the levels of SOD and MDA in H/R-EMVs-treated thoracic aortic rings of rats were measured using SOD and MDA *** H/R-EMVs were induced by H/R-treated HUVECs and isolated by *** membrane vesicles(< 1 urn) induced by H/R were CD144 ***-induced relaxation and NO production of rat thoracic aortic rings were impaired by H/R-EMVs treatment in a concentration-dependent manner(P<0.05,P<0.01).The expression of total eNOS(t-eNOS)was not affected by H/***,the expression of p-eNOS decreased after treated with H/*** activity of SOD decreased and the level of MDA increased in H/R-EMVs treated rat thoracic aortic rings(P<0.01).Conclusion ACh induced endothelium-dependent relaxation of thoracic aortic rings of rats was impaired by H/R-EMVs in a concentration-dependent *** mechanisms included a decrease in NO production,p-eNOS expression and an increase in oxidative stress.

关键词：人脐静脉内皮细胞主动脉大鼠细胞来源微泡复氧缺氧浓度依赖性

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Beneficial effects of date palm fruits on neurodegenerative diseases

引用

Neural Regeneration Research 2016年第7期11卷 1071-1072页

作者： Musthafa Mohamed Essa Mohammed Akbar Mohammed Abdul Sattar Khan Department of Food Science and Nutrition College of Agriculture and Marine SciencesSultan Qaboos University Ageing and Dementia Research Group Sultan Qaboos University section of Molecular pharmacology and Toxicology Laboratory of Membrane Biochemistry and BiophysicsNational Institute on Alcohol Abuse and AlcoholismNational Institutes of Health Department of Anesthesia Critical Care and Pain MedicineShriners Hospitals for Children~?Massachusetts General Hospital and Harvard Medical School

Date palm tree（Phoenix dactylifera L.）is an important crop,which is cultivated in many countries extending from North Africa to the Middle East,including many of the GCC（Gulf Cooperation Council）Countries（Allaith... 详细信息

关键词： fruits Phoenix climatic cultivated Africa neuronal Middle amyloid poorly occlusion

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Enrichment of retinal ganglion and Müller glia progenitors from retinal organoids derived from human induced pluripotent stem cells-possibilities and current limitations

引用

World Journal of Stem Cells 2020年第10期12卷 1171-1183页

作者： Kristine Karla Freude Sarkis Saruhanian Alanna McCauley Colton Paterson Madeleine Odette Annika Oostenink Poul Hyttel Mark Gillies Henriette Haukedal Miriam Kolko Department of Veterinary and Animal Sciences Group of Stem Cell Models for Studies of Neurodegenerative DiseasesSection for Pathobiological SciencesFaculty of Health and Medical SciencesUniversity of CopenhagenFrederiksberg 1870Denmark Department of Drug Design and pharmacology University of CopenhagenCopenhagen 2100Denmark Save Sight Institute South BlockSydney Eye HospitalSydney 2000Australia Department of Ophthalmology Copenhagen University HospitalRigshospitalet-GlostrupGlostrup 2600Denmark

BACKGROUND Retinal organoids serve as excellent human-specific disease models for conditions affecting otherwise inaccessible retinal tissue from *** permit the isolation of key cell types affected in various eye diseases including retinal ganglion cells(RGCs)and Müller *** To refine human-induced pluripotent stem cells(hiPSCs)differentiated into threedimensional(3D)retinal organoids to generate sufficient numbers of RGCs and Müller glia progenitors for downstream *** In this study we described,evaluated,and refined methods with which to generate Müller glia and RGC progenitors,isolated them via magnetic-activated cell sorting,and assessed their lineage stability after prolonged 2D *** progenitor populations were characterized via quantitative PCR and immunocytochemistry,and the ultrastructural composition of retinal organoid cells was *** Our study confirms the feasibility of generating marker-characterized Müller glia and RGC progenitors within retinal *** retinal organoids can be dissociated and the Müller glia and RGC progenitor-like cells isolated via magnetic-activated cell sorting and propagated as *** Enrichment of Müller glia and RGC progenitors from retinal organoids is a feasible method with which to study cell type-specific disease phenotypes and to potentially generate specific retinal populations for cell replacement therapies.

关键词： Human induced pluripotent stem cells Retinal organoids Retinal ganglion cells Müller glia Progenitors Cell-type enrichment

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Drug- and herb-induced liver injury:Progress,current challenges and emerging signals of post-marketing risk

引用

World Journal of Hepatology 2015年第13期7卷 1761-1771页

作者： Emanuel Raschi Fabrizio De Ponti pharmacology Unit Department of Medical and Surgical SciencesAlma Mater StudiorumUniversity of Bologna

Drug-induced liver injury(DILI) and herb-induced liver injury is a hot topic for clinicians,academia,drug companies and regulators,as shown by the steadily increasing number of publications in the past 15 *** review will first provide clues for clinicians to suspect idiosyncratic(unpredictable) DILI and succeed in *** assessment remains challenging and requires careful medical history as well as awareness of multifaceted aspects,especially for *** discontinuation and therapy reconciliation remain the mainstay in patent's management to minimize occurrence of acute liver *** second section will address novel agents associated with liver injury in 2014(referred to as "signals"),especially in terms of clinical,research and drug development *** will be provided into recent trends by highlighting the contribution of different post-marketing data,especially registries and spontaneous reporting *** literature scrutiny suggests:(1) the importance of postmarketing databases as tools of clinical evidence to detect signals of DILI risk;and(2) the need for joining efforts in improving predictivity of pre-clinical assays,continuing post-marketing surveillance and design ad hoc post-authorization safety *** this context,ongoing European/United States research consortia and novel pharmaco-epidemiological tools(e.g.,specialist prescription event monitoring) will support innovation in this *** oral anticoagulants and herbal/dietary supplements appear as key research priorities.

关键词： Hepatotoxicity Liver damage Herb Signal Safety Predictivity

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

治疗2型糖尿病的新药

引用

国外医学（内分泌学分册） 2005年第3期25卷 186-188页

作者： Ole Schmitz Institute of clinical Medicine and pharmacology Aarhus UniversityDenmark

2型糖尿病的发病机理包括β细胞的胰岛素分泌功能异常和胰岛素抵抗。治疗2型糖尿病的药物作用在骨骼肌、肝脏和脂肪组织等胰岛素敏感组织,某些药物还具有中枢和胃肠作用。有一些药物已在动物试验中证明具有β细胞保护功能。胰升糖素样肽... 详细信息

2型糖尿病的发病机理包括β细胞的胰岛素分泌功能异常和胰岛素抵抗。治疗2型糖尿病的药物作用在骨骼肌、肝脏和脂肪组织等胰岛素敏感组织,某些药物还具有中枢和胃肠作用。有一些药物已在动物试验中证明具有β细胞保护功能。胰升糖素样肽1和胰淀素都是具有上述功能的肽类物质。这些药物在未来2～3年将会上市。它们从有效降糖、减少低血糖发生以及提供心血管保护方面,为2型糖尿病治疗提供了诱人前景。

关键词： 2型糖尿病胰升糖素样肽-1 新药胰岛素抵抗胰岛素分泌心血管保护糖尿病治疗功能异常发病机理药物作用敏感组织脂肪组织胃肠作用保护功能动物试验肽类物质 β细胞骨骼肌胰淀素低血糖

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Transgenic PDGF-BB sericin hydrogel potentiates bone regeneration of BMP9-stimulated mesenchymal stem cells through a crosstalk of the Smad-STAT pathways

引用

Regenerative Biomaterials 2023年第1期10卷 181-193页

作者： Hui-Jie Zhang Fu-Shu Li Feng Wang Han Wang Tong-Chuan He Russell R.Reid Bai-Cheng He Qingyou Xia Key Laboratory of Biochemistry and Molecular pharmacology of Chongqing Chongqing Medical UniversityChongqing 400016China Department of pharmacology School of PharmacyChongqing Medical UniversityChongqing 400016China Department of Pharmacy Chongqing Emergency Medical CenterChongqing University Central HospitalChongqing 400014China Biological Science Research Center Chongqing Key Laboratory of Sericultural ScienceChongqing Engineering and Technology Research Center for Novel Silk MaterialsSouthwest UniversityChongqing 400715China Department of Pharmacy Panzhou People’s HospitalGuizhou 553599China Molecular Oncology Laboratory Department of Orthopaedic Surgery and Rehabilitation MedicineThe University of Chicago Medical CenterChicagoIL 60637USA Laboratory of Craniofacial Biology and Development Department of Surgery Section of Plastic SurgeryThe University of Chicago Medical CenterChicagoIL 60637USA

Silk as a natural biomaterial is considered as a promising bone substitute in tissue *** and fibroin are the main components of silk and display unique features for their programmable mechanical properties,biocompatibility,biodegradability and morphological *** has been reported that sericin recombinant growth factors(GFs)can support cell proliferation and induce stem cell differentiation through cross-talk of signaling pathways during tissue *** transgenic technology allows the productions of bioactive heterologous GFs as fusion proteins with sericin,which are then fabricated into solid matrix or hydrogel ***,using an injectable hydrogel derived from transgenic platelet-derived GF(PDGF)-BB silk sericin,we demonstrated that the PDGF-BB sericin hydrogel effectively augmented osteogenesis induced by bone morphogenetic protein(BMP9)-stimulated mesenchymal stem cells(MSCs)in vivo and in vitro,while inhibiting adipogenic *** gene expression and protein-protein interactions studies demonstrated that BMP9 and PDGF-BB synergistically induced osteogenic differentiation through the cross-talk between Smad and Stat3 pathways in ***,our results provide a novel strategy to encapsulate osteogenic factors and osteoblastic progenitors in transgenic sericin-based hydrogel for robust bone tissue engineering.

关键词： biomaterials growth factor BMP9 osteogenesis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Clues from a missense mutation of the adenosine A1 receptor gene associated with early-onset Parkinson’s disease

引用

Neural Regeneration Research 2023年第10期18卷 2204-2205页

作者： Sergi Ferré Leonardo Pardo Francisco Ciruela Integrative Neurobiology section National Institute on Drug AbuseIntramural Research ProgramNational Institutes of HealthBaltimoreMDUSA Laboratory of Computational Medicine Biostatistics UnitFaculty of MedicineUniversitat Autònoma BarcelonaBellaterraBarcelonaSpain pharmacology Unit Department of Pathology and Experimental TherapeuticsSchool of Medicine and Health SciencesInstitute of NeurosciencesUniversity of Barcelona.Neuropharmacology&Pain GroupNeuroscience ProgramBellvitge Institute for Biomedical ResearchL’Hospitalet de LlobregatSpain

Parkinson’s disease (P D) is a com plex neu rodegenerative disorder for which ra re and common genetic variants contribute to disease risk,onset,and *** genetic contribution to PD can be classified mainly in,first,rare DNA variants that are highly penetrant and therefore causal,which are typically associated with monogenic PD;and second,more common risk polymorphic variants,which individually exert a small increase in the risk of the disease,which are usually identified in the most prevalent and apparently sporadic PD (Blauwendraat et al.,2020).

关键词： typically degenerative

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

2004英国高血压学会高血压治疗指南(BHS-IV):概要

引用

英国医学杂志中文版 2004年第5期7卷 273-279页

作者： Bryan Williams Neil R Poulter Peter S Sever Morris J Brown Mark Davis Gordon T McInnes John F Potter 邱洪 Department of Cardiovascular Sciences University of Leicester Clinical Sciences Building Leicester Royal Infirmary Leicester professor of medicine International Centre for Circulatory Health Imperial College London and St Mary's Hospital London professor of preventive cardiovascular medicine International Centre for Circulatory Health Imperial College London and St Mary's Hospital London Peter S Sever professor of clinical pharmacology Simon McG Thom reader in medicine clinical pharmacology Unit Addenbrooke's Hospital University of Cambridge Cambridge professor of clinical pharmacology Moorfield House Surgery Garforth Leeds general practitioner section of clinical pharmacology and Stroke Medicine Division of Cardiovascular and Medical Sciences Gardiner Institute Western Infirmary University of Glasgow Glasgow professor of clinical pharmacology Ageing and Stroke Medicine section Department of Cardiovascular Sciences Glenfield Hospital University of Leicester Leicester professor of ageing and stroke medicine the BHS guidelines working party for the British Hypertension Society

在高血压领域，不断出现的新证据已日益受到关注。例如：血压可作为心血管疾病的一个危险因素；改进生活方式对于预防和治疗高血压的重要意义；不同类型药物的有效性和安全性；高危高血压患者包括糖尿病患者的治疗；心血管疾病总体危险... 详细信息

在高血压领域，不断出现的新证据已日益受到关注。例如：血压可作为心血管疾病的一个危险因素；改进生活方式对于预防和治疗高血压的重要意义；不同类型药物的有效性和安全性；高危高血压患者包括糖尿病患者的治疗；心血管疾病总体危险性评估的重要性以及使用他汀类药物所获得的额外益处。

关键词：高血压患者治疗指南心血管疾病高危预防和治疗他汀类药物糖尿病患者概要英国有效性

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effect of Collagen Type I or Human Fibronectin on Imatinib Cytotoxicity in Oral Squamous Cell Carcinoma

引用

pharmacology & Pharmacy 2016年第7期7卷 255-263页

作者： Masahiko Morioka Mai Hazekawa Tomoyo Kawakubo-Yasukochi Takuya Nishinakagawa Seiji Nakamura Manabu Nakashima Department of Immunological and Molecular pharmacology Faculty of Pharmaceutical Science Fukuoka University Fukuoka Japan section of Oral and Maxillofacial Oncology Division of Maxillofacial Diagnostic and Surgical Sciences Faculty of Dental Science Kyushu University Fukuoka Japan

Extracellular matrix (ECM) components are critical for all aspects of cell proliferation, adhesion, and morphological alteration. Recent progress has yielded multiple molecular drugs that specifically target gene products which are expressed at high levels in tumor cells. We investigated whether the sensitivity of tumor cells to molecular target drugs could be altered when cells were cultured on surfaces with various coating conditions such as lysine, laminin, Matrigel, collagen type I, and human fibronectin (HFN). This study evaluates the IC50 values of imatinib in oral squamous cell carcinoma (OSCC) cell lines when cells are cultured on plates coated with ECM components such as collagen type I and HFN. Four OSCC cell lines—SQUU-A, SQUU-B, SAS, and NA— are used. Cell proliferation was assessed using WST-8 reagent. Collagen type I and HFN significantly enhanced OSCC cell proliferation compared with control. Imatinib cytotoxicity was demonstrated following culture of OSCCs in culture plates coated with collagen type I or HFN. However, there were no significant changes in imatinib IC50 values between collagen type I and HFN. These results indicate that some molecular target drugs exhibit cancer cell cytotoxicity without being influenced by cell environment factors such as the ECM. These results may aid in the search for molecular target drugs to apply in the clinical chemotherapy of OSCC.

关键词： Collagen Type I Human Fibronectin Imatinib Cytotoxicity Oral Squamous Cell Carcinoma

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Pharmacological characterizationof synthetic cannabinoid MAM-2201：radioligand binding and abuse-related effects

引用

中国药理学与毒理学杂志 2017年第10期31卷 1016-1017页

作者： William E FANTEGROSSI Aaron JANOWSKY Amy J ESHLEMAN Lauren N RUSSELL Saki FUKUDA Jyoti GOGOI Cassandra PRIOLEAU Ambuja S BALE Srihari R TELLA Merle G PAULE Takato HIRANITA Department of pharmacology and Toxicology College of Medicine University of Arkansas for Medical Sciences AR 72207 Division of Neurotoxicology National Center for Toxicological Research Food and Drug Administration AR 72079 Departments of Psychiatry and Behavioral Neuroscience Oregon Health & Science University Portland OR 97239-3098 VA Portland Health Care System Portland OR 97239 Drug and Chemical Evaluation section Drug Enforcement Administration

OBJECTIVE Over 30% of all new psychoactive substances identified by the UN Office on Drugs and Crime in 2016 were synthetic *** recent emergence of MAM-2201 on the illicit market is troubling because this drug has no precedent in either the scientific or patent literature,and appears to be a novel compound developed specifically as a "graymarket" drug of abuse bystructurally combining the known synthetic cannabinoids JWH-122 and *** is currently no published information regarding the pharmacology of *** The present studies characterized cannabinoid-like effects of MAM-2201 in vitro(interactions with cannabinoid type 1 receptors[CB1 Rs]) and in vivo(in mice and rats).RESULTS In a radioligand binding assay using [3 H]CP55,940 in HEK cell membranes transfected with the CB1 R,MAM-2201(K i=5.4 nmol·L^(-1)),had higher binding affinity than WIN 55,212-2(K i=80 nmol·L^(-1)),and D9-THC(K i=8.3 nmol·L^(-1)).The E max values for MAM-2201 and WIN 55,212-2 in an assay of agonist inhibition of forskolin-stimulated c AMP were 85%(EC50=0.45 nmol·L^(-1)) and 95%,respectively,as compared with the D9-THC E max of 74%.In mice,MAM-2201(0.003-1.0 mg·kg^(-1),IP) produced dose-dependent cannabimimetic effects which were both more potent and more effective than those of ***-2201 and D9-THC dose-dependently produced hypothermia:ED50=0.287 and 25.4 mg·kg^(-1),analgesia:ED50=0.125 and 29.4 mg·kg^(-1),and catalepsy:ED50=0.301 and18.9 mg·kg^(-1) in adult male CD1 ***,MAM-2201 also elicited convulsant effects at a dose of 1.0 mg·kg^(-1) in 8/8 murine *** rats,MAM-2201 produced dose-dependent D9-THC-like interoceptive effects in subjects trained to discriminate 3.0 mg·kg^(-1)(IP) D9-THC from *** MAM-2201 binds CB1 Rs with high affinity and agonist efficacy,and functions as a potent cannabinoid agonist in vivo across several complementary measures of cannabinoid activity in two rodent species.

关键词： cannabinoid CB1 receptor behavior abuse liability

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：