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Apoptosis Induced by Zinc Deficiency in Rat Osteoblast:Possible Involvement of Protein Kinase C

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Biomedical and Environmental sciences 1999年第3期12卷 101-109页

作者： CEN XIAO-BO WANG RUI-SHU AND WANG HANG(Medical Experimeatel Center, the First Hospital of West China University of Medical sciences (WCUMS), Chengdu 610041, China Derpartment of Public of Health (WCUMS) Department of Stomatology (WCUMS)) Medical experimental Center First Hosp. W. China Univ. Med. Sci. Chengdu 610041 China

Ra ostcobasts were isolated from the 21-day fetal rat calvchas. The cells were grown in DMEM Plus 10% FBS, and were treated for 24 h. with 10 μmol/L TPEN or 10 μmol/L TPEN supplemented with 10 μmol/L Zn2+. Apoptosis of osteoblasts were measured by fiow cytometry, electron microscopy and DNA fragmentation analyzed by gel elecmphoresis. In addition, IP3 production and PKC activity were measmed in ordr to show whether they are involved in apoptosis in osteoblast induced by alnc deficiency. The results showed that 10 μmol/L TPEN could induce apoptosis in osteoblast in 24 h. But cells ed with 10μmol/L TPEN supplemented with 10 μmol/L Zn2+ showed no apoptotic changs in 24 h. TPEN significantly reduced the formation of IP3 and PKC activity after 24 h incubation. No differences were observed between the cells treated with TPEN supPlemented with Zn2+ simulaneosly and the untreated cells. It can be inferred that apoptosis induced by ainc deficiency may be due to the decreased activity of PKC which is impaired by reduced formation of IP3.

关键词： Apoptosis Induced by Zinc Deficiency in Rat Osteoblast Cell cell

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Comparable anti-CMV responses of transplant donor and third-party CMV-specific T cells for treatment of CMV infection after allogeneic stem cell transplantation

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Cellular & Molecular Immunology 2022年第4期19卷 482-491页

作者： Xu-Ying Pei Xue-Fei Liu Xiang-Yu Zhao Meng Lv Xiao-Dong Mo Ying-Jun Chang Qian-Nan Shang Yu-Qian Sun Yu-Hong Chen Lan-Ping Xu Yu Wang Xiao-Hui Zhang Kai-Yan Liu Xiao-Jun Huang Peking University People’s Hospital Peking University Institute of HematologyNational Clinical Research Center for Hematologic DiseaseBeijing Key Laboratory of Hematopoietic Stem Cell TransplantationBeijing100044China Peking-Tsinghua Center for Life sciences Beijing100871China

Adoptive transfer of cytomegalovirus(CMV)-specific cytotoxic T lymphocytes(CMV-CTLs)from original transplant donors or third-party donors was effective for the treatment of CMV infection after allogenic stem cell transplantation(allo-SCT),but the antiviral activity of CMV-CTL types has not been *** determine whether third-party CMV-CTLs provide comparable long-term antiviral efficacy to transplant donor CMV-CTLs,we first compared the antiviral abilities of transplant donors and third-party CMV-CTLs for treatment of CMV infection in two mouse models,compared the in vivo recovery of CMV-specific immunity,and analyzed the underlying mechanisms driving sustained antiviral *** results showed that both donor and third-party CMV-CTLs effectively combated systemic CMV infection by reducing CMV pathology and tumor burden 28 days *** in vivo recovery of CMV-specific immunity after CMV-CTL infusion was comparable in both groups.A detailed analysis of the source of recovered CMV-CTLs showed the proliferation and expansion of graft-derived endogenous CMV-CTLs in both *** clinical study,which enrolled 31 patients who received third-party CMV-CTLs and 62 matched pairs of individuals who received transplant donor CMV-CTLs for refractory CMV infection,further showed that adoptive therapy with donor or third-party CMV-CTLs had comparable clinical responses without significant therapy-related *** observed strong expansion of CD8+tetramer+T cells and proliferation of recipient endogenous CMV-CTLs after CMV-CTL infusion,which were associated with a reduced or cleared viral *** data confirmed that adoptive therapy with third-party or transplant donor CMV-CTLs triggered comparable antiviral responses to CMV infection that might be mediated by restoration of endogenous CMV-specific immunity.

关键词： Allogeneic stem cell transplantation CMV-specific cytotoxic T lymphocytes Transplant donor Third party donor Immunotherapy

Prevention of diabetic microangiopathy by prophylactic transplant of mobilized peripheral blood mononuclear cells

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Acta Pharmacologica Sinica 2007年第1期28卷 89-97页

作者： Bin ZHOU~(2,5) Xiao-cang CAO~(2,3,5) Zhi-hong FANG~2 Cui-lin ZHENG~2 Zhi-bo HAN~2 He REN2, Man-chiu POON~(2,4) Zhong-chao HAN~(2,6)2 State Key Laboratory of experimental Hematology,National Research Center for Stem Cell Engineering and Technology,Institute of Hematology,Chinese Academy of Medical sciences and Peking Union Medical College,Tianjin 300020, China 3 Department Gastroenterology,TMU,General Hopital of Tianjin Medical University,Tianjin 300052,China 4 Departments of Medicine,University of Calgary,Alberta T2N 2T9,Canada State Key Laboratory of experimental Hematology National Research Center for Stem Cell Engineering and Technology Institute of Hematology Chinese Academy of Medical Sciences and Peking Union Medical College Tianjin China Department Gastroenterology TMU General Hopital of Tianjin Medical University Tianjin China Departments of Medicine University of Calgary Alberta Canada

Aim:To investigate whether the prophylactic local delivery of mobilized periph-eral blood mononuclear cells(M-PBMNC)could prevent peripheral microangio-pathy in diabetic nude ***:Diabetic nude mice were induced withintraperitoneal injections of *** the time course of diabetes,wedetected the capillary and arteriole density of mice adductor muscles by *** situ apoptosis was detected by using TdT-mediated dUTP nickend labeling(TUNEL)methods.M-PBMNC were labeled and locally delivered tothe adductor *** cells were also isolated and cultured in vitrofor the detection and counting of endothelial progenitor cells(EPC).Results:Rarefication of capillaries and arterioles,enhanced apoptosis in adductor muscles,and reduced circulating EPC in diabetic nude *** local delivery ofM-PBMNC halted the progression of microvascular rarefaction in hind-limb skel-etal muscles by inhibiting *** detected the survival,migration andincorporation of transplanted M-PBMNC into the murine vasculature in ***,more EPC were available from M-PBMNC than non-mobilized ***:These results suggested that the prophylactic local delivery ofM-PBMNC may represent a novel approach for the treatment of microvascularcomplications in diabetics.

关键词： prevention diabetic angiopathis transplan tation endothelial cells stem cells

Novel therapies targeting hypoxia mechanism to treat pancreatic cancer

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Chinese Journal of Cancer Research 2021年第2期33卷 216-231页

作者： Wenhao Luo Jiangdong Qiu Lianfang Zheng Taiping Zhang Department of General Surgery Peking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China Department of Nuclear Medicine Peking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China clinical Immunology Center Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China

Pancreatic cancer(PC)is one of the deadliest *** high mortality rate of PC largely results from delayed diagnosis and early ***,identifying novel treatment targets for patients with PC is urgently required to improve survival rates.A major barrier to successful treatment of PC is the presence of a hypoxic tumor microenvironment,which is associated with poor prognosis,treatment resistance,increased invasion and *** studies have identified a number of novel molecules and pathways in PC cells that promote cancer cells progression under hypoxic conditions,which may provide new therapy strategies to inhibit the development and metastasis of *** review summarizes the latest research of hypoxia in PC and provides an overview of how the current therapies have the capacity to overcome hypoxia and improve PC patient *** findings will eventually provide guidance for future PC management and clinical trials and hopefully improve the survival of patients with PC.

关键词： Pancreatic cancer hypoxia tumor microenvironment novel strategies survival

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Ophthalmologic manifestations of celiac disease

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International Journal of Ophthalmology(English edition) 2016年第1期9卷 159-162页

作者： Thiago Goncalves dos Santos Martins Ana Luiza Fontes de Azevedo Costa Maria Kiyoko Oyamada Paulo Schor Aytan Miranda Sipahi Department of Ophthalmology Federal University of SgoPaulo Sao Paulo 04023-062 Brazil Department of Ophthalmology University of Sao Paulo Sao Paulo 04023-062 Brazil Departmem of Gastroenterology Clinicas Hospital of University of Sao Paulo and the Laboratory of Experimental Clinical Gastroenterology School of Medicine at the University ofSao Paulo LIM 07 Sao Paulo 04023-062 Brazil

Celiac disease is an autoimmune disorder that affects the small intestine of genetically predisposed *** manifestations are within the extra-intestinal manifestations,and can be divided into those of autoimmune disorders or those due to absorptive *** article reviewed the ophthalmologic manifestation of celiac *** symptoms are rare,but should be investigated in patients with celiac disease and taken into consideration as the first systemic manifestation.

关键词： ophthalmology celiac disease review

The Plant Vascular System: Evolution, Development and Functions

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Journal of Integrative Plant Biology 2013年第4期55卷 294-388页

作者： William J. Lucas Andrew Groover Raffael Lichtenberger Kaori Furuta Shri-Ram Yadav Yk Helariutta Xin-Qiang He Hiroo Fukuda Julie Kang Siobhan M. Brady John W. Patrick John Sperry Akiko Yoshida Ana-Flor López-Millan Michael A. Grusak Pradeep Kachroo Department of Plant Biology College of Biological Sciences University of California US Forest Service Pacific SW Res Stn Institute for Forest Genetics Institute of Biotechnology/Department of Biology and Environmental sciences University of Helsinki State Key Laboratory of Protein and Plant Gene Research College of Life Sciences Peking University Department of Biological sciences Graduate School of Science The University of Tokyo Biology Department University of Northern Iowa School of Environmental and Life sciences The University of Newcastle Biology Department University of Utah Plant Nutrition Department Aula Dei Experimental Station (CSIC) USDA-ARS Children's Nutrition Research Center Department of Pediatrics Baylor College of Medicine Department of Plant Pathology College of Agriculture University of Kentucky

The emergence of the tracheophyte-based vascular system of land plants had major impacts on the evolution of terrestrial biology, in general, through its role in facilitating the development of plants with increased stature, photosynthetic output, and ability to colonize a greatly expanded range of environmental habitats. Recently, considerable progress has been made in terms of our understanding of the developmental and physiological programs involved in the formation and function of the plant vascular system. In this review, we first examine the evolutionary events that gave rise to the tracheophytes, followed by analysis of the genetic and hormonal networks that cooperate to orchestrate vascular development in the gymnosperms and angiosperms. The two essentialfunctions performed by the vascular system, namely the delivery of resources （water, essential mineral nutrients, sugars and amino acids） to the various plant organs and provision of mechanical support are next discussed. Here, we focus on critical questions relating to structural and physiological properties controlling the delivery of material through the xylem and phloem. Recent discoveries into the role of the vascular system as an effective long-distance communication system are next assessed in terms of the coordination of developmental, physiological and defense-related processes, at the whole-plant level. A concerted effort has been made to integrate all these new findings into a comprehensive picture of the state-of-the-art in the area of plant vascular biology. Finally, areas important for future research are highlighted in terms of their likely contribution both to basic knowledge and applications to primary industry.

关键词： Evolution vascular development phloem xylem nutrient delivery long-distance communication systemic signaling.

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Neuropathological characteristics of abnormal white matter functional signaling in adolescents with major depression

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World Journal of Psychiatry 2024年第2期14卷 276-286页

作者： Xin-Lin Huang Ju Gao Yong-Ming Wang Feng Zhu Jing Qin Qian-Nan Yao Xiao-Bin Zhang Hong-Yan Sun Imaging and Nuclear Medicine Jiamusi UniversityJiamusi 154000Heilongjiang ProvinceChina Department of Psychiatry The Affiliated Guangji Hospital of Soochow UniversitySuzhou 215137Jiangsu ProvinceChina School of Biology&Basic Medical sciences Medical College of Soochow UniversitySuzhou 215137Jiangsu ProvinceChina Department of Radiology Shanghai Anting HospitalShanghai 20000China Department of Radiology The Affiliated Guangji Hospital of Soochow UniversitySuzhou 215137Jiangsu ProvinceChina

BACKGROUND Major depression disorder(MDD)constitutes a significant mental health *** surveys indicate that the lifetime prevalence of depression in adolescents is much higher than that in adults,with a corresponding increased risk of *** studying brain dysfunction associated with MDD in adolescents,research on brain white matter(WM)is *** researchers even mistakenly regard the signals generated by the WM as noise *** fact,studies have shown that WM exhibits similar blood oxygen level-dependent signal *** alterations in WM signals and their relationship with disease severity in adolescents with MDD remain *** To explore potential abnormalities in WM functional signals in adolescents with *** This study involved 48 adolescent patients with MDD and 31 healthy controls(HC).All participants were assessed using the Patient Health Questionnaire-9 Scale and the mini international neuropsychiatric interview(MINI)suicide *** addition,a Siemens Skyra 3.0T magnetic resonance scanner was used to obtain the subjects'image *** DPABI software was utilized to calculate the WM signal of the fractional amplitude of low frequency fluctuations(fALFF)and regional homogeneity,followed by a two-sample t-test between the MDD and HC *** component analysis(ICA)was also used to evaluate the WM functional ***’s correlation was performed to assess the relationship between statistical test results and clinical *** Compared to HC,individuals with MDD demonstrated a decrease in the fALFF of WM in the corpus callosum body,left posterior limb of the internal capsule,right superior corona radiata,and bilateral posterior corona radiata[P<0.001,family-wise error(FWE)voxel correction].The regional homogeneity of WM increased in the right posterior limb of internal capsule and left superior corona radiata,and decreased in the left superior longitudinal fasciculus(P<0.001,FWE voxel corre

关键词： White matter Regional homogeneity The fractional amplitude of low-frequency fluctuations Independent component analysis Adolescents Major depression disorders

Epidemiology and risk factors for mortality in critically ill patients with pancreatic infection

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Journal of Intensive Medicine 2024年第1期4卷 81-93页

作者： Marie Dejonckheere Massimo Antonelli Kostoula Arvaniti Koen Blot Ben CreaghBrown Dylan Wde Lange Jan De Waele Mieke Deschepper Yalim Dikmen George Dimopoulos Christian Eckmann Guy Francois Massimo Girardis Despoina Koulenti Sonia Labeau Jeffrey Lipman Fernando Lipovestky Emilio Maseda Philippe Montravers Adam Mikstacki JoseArtur Paiva Cecilia Pereyra Jordi Rello JeanFrancois Timsit Dirk Vogelaers Stijn Blot the Abdominal Sepsis Study(AbSeS)group on behalf of the Trials Group of the European Society of Intensive Care Medicine Department of Internal Medicine and Pediatrics Faculty of Medicine and Health SciencesGhent UniversityGhentBelgium Department of Anesthesiology Intensive Care and Emergency MedicineFondazione Policlinico Universitario A.Gemelli IRCCSRomeItaly UniversitàCattolica del Sacro Cuore RomeItaly Intensive Care Unit Papageorgiou University Affiliated HospitalThessaloníkiGreece Department of Epidemiology and Public Health SciensanoIxellesBelgium Surrey Perioperative Anaesthetic Critical Care Collaborative Research Group(SPACeR) Royal Surrey County HospitalGuildfordUK Department of clinical and experimental Medicine University of SurreyGuildfordUK Department of Intensive Care Medicine University Medical Center UtrechtUniversity UtrechtUtrechtthe Netherlands Department of Intensive Care Medicine Ghent University HospitalGhentBelgium Data Science Institute Ghent University HospitalGhentBelgium Department of Anesthesiology and Reanimation Cerrahpasa School of MedicineIstanbul University-CerrahpasaIstanbulTurkey rd Department of Critical Care “EVGENIDIO”HospitalNational and Kapodistrian University of AthensAthensGreece Department of General Visceral and Thoracic SurgeryKlinikum Hannoversch-MuendenGoettingen UniversityGöttingenGermany Division of Scientific Affairs-Research European Society of Intensive Care MedicineBrusselsBelgium Anesthesia and Intensive Care Department University Hospital of ModenaModenaItaly UQ Centre for clinical Research Faculty of MedicineThe University of QueenslandBrisbaneQLDAustralia nd Critical Care Department Attikon University HospitalAthensGreece Department of Nursing Faculty of EducationHealth and Social WorkUniversity College GhentGhentBelgium Jamieson Trauma Institute The University of QueenslandBrisbaneQLDAustralia Nimes University Hospital University of MontpellierNimesFrance Critical Care Department Hospital of the Interamerican Open University(UAI)Buenos AiresArgentina Surgical Critical Care Department of AnesthesiaHospital Universitario

Background:The AbSeS-classification defines specific phenotypes of patients with intra-abdominal infection based on the(1)setting of infection onset(community-acquired,early onset,or late-onset hospital-acquired),(2)presence or absence of either localized or diffuse peritonitis,and(3)severity of disease expression(infection,sepsis,or septic shock).This classification system demonstrated reliable risk stratification in intensive care unit(ICU)patients with intra-abdominal *** study aimed to describe the epidemiology of ICU patients with pancreatic infection and assess the relationship between the components of the AbSeS-classification and ***:This was a secondary analysis of an international observational study(“AbSeS”)investigating ICU patients with intra-abdominal *** patients with pancreatic infection were included in this analysis(n=165).Mortality was defined as ICU mortality within 28 days of observation for patients discharged earlier from the *** with mortality were assessed using logistic regression analysis and reported as odds ratio(OR)and 95%confidence interval(CI).Results:The overall mortality was 35.2%(n=58).The independent risk factors for mortality included older age(OR=1.03,95%CI:1.0 to 1.1 P=0.023),localized peritonitis(OR=4.4,95%CI:1.4 to 13.9 P=0.011),and persistent signs of inflammation at day 7(OR=9.5,95%CI:3.8 to 23.9,Pclinically relevant differences in microbial etiology between survivors and ***:In pancreatic infection,a challenging source/damage control and ongoing pancreatic inflammation appear to be the strongest contributors to an unfavorable short-term *** this limited series,essentials of the AbSeS-classification,such as the setting of infection onset,diffuse

关键词： Pancreatic infection Intensive care unit Mortality Intra-abdominal infection Sepsis

Atrophic gastritis and gastric cancer tissue miRNome analysis reveals hsa-miR-129-1 and hsa-miR-196a as potential early diagnostic biomarkers

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World Journal of Gastroenterology 2022年第6期28卷 653-664页

作者： Greta Varkalaite Evelina Vaitkeviciute Ruta Inciuraite Violeta Salteniene Simonas Juzenas Vytenis Petkevicius Rita Gudaityte Antanas Mickevicius Alexander Link Limas Kupcinskas Marcis Leja Juozas Kupcinskas Jurgita Skieceviciene Institute for Digestive Research Lithuanian University of Health SciencesKaunas 44307Lithuania Department of Gastroenterology Lithuanian University of Health SciencesKaunas 44307Lithuania Department of Surgery Lithuanian University of Health SciencesKaunas 44307Lithuania Department of Gastroenterology Hepatology and Infectious DiseasesOttovon-Guericke University HospitalMagdeburg 39120Germany Institute of clinical and Preventive Medicine&Faculty of Medicine University of LatviaRiga 1586Latvia

BACKGROUND Gastric cancer(GC)is one of the most frequently diagnosed tumor *** most cases,GC develops in a stepwise manner from chronic gastritis or atrophic gastritis(AG)to *** of the major issues in clinical settings of GC is diagnosis at advanced disease stages resulting in poor *** RNAs(mi RNAs)are small noncoding molecules that play an essential role in a variety of fundamental biological ***,clinical potential of mi RNA profiling in the gastric cancerogenesis,especially in premalignant GC cases,remains *** To evaluate the AG and GC tissue mi RNomes and identify specific mi RNAs’potential for clinical applications(e.g.,non-invasive diagnostics).METHODS Study included a total of 125 subjects:Controls(CON),AG,and GC *** study subjects were recruited at the Departments of Surgery or Gastroenterology,Hospital of Lithuanian University of Health sciences and divided into the profiling(n=60)and validation(n=65)*** RNA isolated from tissue samples was used for preparation of small RNA sequencing libraries and profiled using next-generation sequencing(NGS).Based on NGS data,deregulated mi RNAs hsa-mi R-129-1-3 p and hsa-mi R-196 a-5 p were analyzed in plasma samples of independent cohort consisting of CON,AG,and GC *** level of hsa-mi R-129-1-3 p and hsa-mi R-196 a-5 p was determined using the quantitative real-time polymerase chain reaction and 2-ΔΔCt *** Results of tissue analysis revealed 20 differentially expressed mi RNAs in AG group compared to CON group,129 deregulated mi RNAs in GC compared to CON,and 99 altered mi RNAs comparing GC and AG *** 2 mi RNAs(hsa-mi R-129-1-3 p and hsa-mi R-196 a-5 p)were identified to be step-wise deregulated in healthy-premalignant-malignant *** under the curve(AUC)-receiver operating characteristic analysis revealed that expression level of hsa-mi R-196 a-5 p is significant for discrimination of CON vs AG,CON vs GC and A

关键词： Gastric cancer Atrophic gastritis Tumorigenesis Helicobacter pylori MicroRNAs Biomarkers