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Expansion of human umbilical cord derived mesenchymal stem cells in regenerative medicine

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World Journal of Stem Cells 2024年第4期16卷 410-433页

作者： Shafiqa Naeem Rajput Bushra Kiran Naeem Anwar Ali Asmat Salim Irfan Khan dr.Panjwani Center for Molecular Medicine and drug Research International Center for Chemical and Biological SciencesUniversity of KarachiKarachi 75270SindhPakistan Surgical Unit 4 Dr.Ruth KM Pfau Civil HospitalKarachi 74400Pakistan Department of Physiology University of KarachiKarachi 75270Pakistan Center for Regenerative Medicine and Stem Cells Research and Department of Ophthalmology and Visual SciencesThe Aga Khan UniversityKarachi 74800SindhPakistan

BACKGROUND Stem cells are undifferentiated cells that possess the potential for self-renewal with the capacity to differentiate into multiple *** humans,their limited numbers pose a challenge in fulfilling the necessary demands for the regeneration and repair of damaged tissues or *** suggested that mesenchymal stem cells(MSCs),necessary for repair and regeneration via transplantation,require doses ranging from 10 to 400 million ***,the limited expansion of MSCs restricts their therapeutic *** To optimize a novel protocol to achieve qualitative and quantitative expansion of MSCs to reach the targeted number of cells for cellular transplantation and minimize the limitations in stem cell therapy *** Human umbilical cord(hUC)tissue derived MSCs were obtained and *** cultured cells were subjected to the following evaluation pro-cedures:Immunophenotyping,immunocytochemical staining,trilineage differentiation,population doubling time and number,gene expression markers for proliferation,cell cycle progression,senescence-associatedβ-galactosidase assay,human telomerase reverse transcriptase(hTERT)expression,mycoplasma,cytomegalovirus and endotoxin *** Analysis of pluripotent gene markers Oct4,Sox2,and Nanog in recultured hUC-MSC revealed no significant *** immunophenotypic markers CD90,CD73,CD105,CD44,vimentin,CD29,Stro-1,and Lin28 were positively expressed by these recultured expanded MSCs,and were found negative for CD34,CD11b,CD19,CD45,and *** recultured hUC-MSC population continued to expand through passage *** gene expression of Pax6,BMP2,and TGFb1 showed no significant variation between recultured hUC-MSC ***,a significant increase(P<0.001)in the mitotic phase of the cell cycle was observed in recultured *** senescence markers(hTERT expression andβ-galactosidase activity)did not show any negative effect on recultured hUC-

关键词： Human umbilical cord Mesenchymal stem cells Expansion Cell proliferation In vitro expansion Senescence

Delay Times and Clinical Outcomes in Acute Myocardial Infarction: Comparison of Periods before and during the COVID-19 Pandemic—Myocardial Infarction and the Pandemic

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World Journal of Cardiovascular Diseases 2024年第6期14卷 392-400页

作者： Clarice Teixeira silva Araújo Arnon Salviato Mameri Thúlio Carrera Guarçoni Venturini Marcus Tadeu Aguilar Constantino Matos Igor Morais Araujo Lopes Luiz Gustavo Ribeiro de Carvalho Murad Cleberson Duartes Ovani Guilherme Vassalo Morais Fernanda Venturini de Castro Danielle Lopes Rocha Lucas Crespo de Barros Rodolfo Costa Sylvestre Luiz Fernando Machado Barbosa Roberto Ramos Barbosa Internal Medicine Universidade Federal do Esprito Santo (UFES) Vitria Brazil hospital Santa Casa de Misericrdia de Vitria Vitria Brazil hospital Santa Rita de Cssia Vitria Brazil hospital Estadual dr. Jayme Santos Neves Serra Brazil Grupo Kora Sade Vitria Brazil hospital Estadual Infantil e Maternidade Alzir Bernardino Alves (HIMABA) Vila Velha Brazil

Introduction: At the beginning of the COVID-19 pandemic, a drop in the number of patients treated for cardiac emergencies raised concern about cardiovascular mortality in that period. An increase in care delay for patients with ST-segment elevation myocardial infarction (STEMI) may have affected clinical outcomes. Objectives: To analyze delay times and clinical outcomes of patients with STEMI undergoing primary percutaneous coronary intervention (PPCI), before and during the COVID-19 pandemic. Methods: Retrospective observational study that included patients with STEMI undergoing PPCI from December 2018 to July 2021. The COVID-19 pandemic cases were divided into two groups: pandemic I—from March to August 2020;and pandemic II—from September 2020 to July 2021. Patients were compared according to the period of hospitalization. Primary outcomes were delay times in assistance and clinical outcomes (acute kidney injury [AKI], post-procedural vascular complications and in-hospital mortality). Results: 108 patients were included, 39 (36.1%) in the pre-pandemic period, 13 (12.1%) in pandemic I and 56 (51.8%) in pandemic II. Time from onset of symptoms to arrival at the service and door-to-balloon time did not differ significantly among groups. Vascular complications were more frequent during the pandemic (I and II) than in the pre-pandemic period (2.5% pre-pandemic vs 15.4% pandemic vs 12.5% pandemic II;p = 0.03). AKI incidence was similar in all three periods. There was a non-significant increase in in-hospital mortality during the COVID-19 pandemic. Conclusion: In patients with STEMI, there was an increase in vascular complications and a trend toward increased mortality during the COVID-19 pandemic. Delay times to admission and reperfusion did not differ significantly between before and during the pandemic.

关键词： Myocardial Infarction Primary Percutaneous Coronary Intervention Coronary Reperfusion Pandemic COVID-19

Point-of-care ultrasonography spotlight:Could venous excess ultrasound serve as a shared language for internists and intensivists?

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World Journal of Critical Care Medicine 2024年第2期13卷 126-136页

作者： Anosh Aslam Khan Hasham Saeed Ibtehaj Ul Haque Ayman Iqbal Doantrang Du Abhilash Koratala Department of Internal Medicine Monmouth Medical CenterLong BranchNJ 07740United States Department of Internal Medicine Trinitas Regional Medical CenterElizabethNJ 07202United States Department of Anesthesiology Dr.Ruth K M Pfau Civil HospitalKarachi 74400Pakistan Department of Internal Medicine Dow Medical CollegeKarachi 74200Pakistan Division of Nephrology Medical College of WisconsinMilwaukeeWI 53226United States

Point-of-care ultrasonography(POCUS),particularly venous excess ultrasound(VExUS)is emerging as a valuable bedside tool to gain real-time hemodynamic *** modality,derived from hepatic vein,portal vein,and intrarenal vessel Doppler patterns,offers a scoring system for dynamic venous congestion *** an assessment can be crucial in effective management of patients with heart failure *** facilitates diagnosis,quantification of congestion,prognostication,and monitoring the efficacy of decongestive *** such,it can effectively help to manage cardiorenal syndromes in various clinical *** or eVExUS explores additional veins,potentially broadening its *** VExUS demonstrates promising outcomes,challenges persist,particularly in cases involving renal and liver parenchymal disease,arrhythmias,and situations of pressure and volume overload *** in utilizing spectral Doppler is pivotal for clinicians to effectively employ this ***,the integration of POCUS,especially advanced applications like VExUS,into routine clinical practice necessitates enhanced training across medical specialties.

关键词： Point-of-care ultrasonography Ultrasound Venous excess ultrasound Doppler Congestion Heart failure

Food allergy among Mexican infants and preschoolers:prevalence and associated factors

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World Journal of Pediatrics 2023年第4期19卷 401-405页

作者： Martín Bedolla-Barajas Jaime Morales-Romero Rafael Sánchez-Magallón Jorge Alejandro Valdez-Soto Tonatiuh Ramses Bedolla-Pulido Carlos Meza-López Allergy and Immunology Department Nuevo Hospital Civil de Guadalajara Dr.Juan I.MenchacaSalvador Quevedo y Zubieta No.740Colonia La Perla44340GuadalajaraJaliscoMéxico Institute of Public Health Universidad VeracruzanaXalapaMexico Pediatrics Department Nuevo Hospital Civil de Guadalajara Dr.Juan I.MenchacaGuadalajaraMexico

The number of people with food allergy(FA)has increased in recent decades,as has the number of foods to which they are *** of FA prevalence vary widely,likely because of different definitions,evaluated foods and geo-graphic *** developed countries,overall preva-lence estimates range from 1.9%to 7.2%[1,2].In Latin America,the prevalence of FA in children is between 1.4%and 5.3%[3-6],with important age-based variations(from 1.9%in infants to 0.4%in preschoolers)[7].FA is a complex trait in which the interplay of genetic and environmental fac-tors results in disease ***-known factors that increase atopic susceptibility include sex[8,9],age[10],and socioeconomic status[11-13].Furthermore,the asso-ciation between family history of allergies and childhood FA has been consistently reported in recent ***,a maternal history of allergic disease appears to be more strongly associated with FA than paternal history of atopy[14].

关键词： prevalence allergy allergic

Literature review on earthen vernacular heritage: contributions to a referential framework

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Built Heritage 2022年第2期6卷 43-54页

作者： Gilberto Carlos Telma Ribeiro Maddalena Achenza Cristina Cruz Ferreira de Oliveira Humberto Varum Gallaecia Architecture and Multimedia Department UPT-Portucalense UniversityR.Dr.António Bernardino de Almeida 5414200-072 PortoPortugal Department of civil Engineering Environment and ArchitecureCagliari University09123 CagliariItaly CONSTRUCT-LESE Polytechnic Institute of SetúbalBarreiro School of TechnologyRua Américo da Silva Marinho2839-001 LavradioPortugal Faculty of Engineering Civil Engineering DepartmentCONSTRUCT-LESEUniversity of PortoRua Dr.Roberto Frias4200-465 PortoPortugal

The state of the art of earthen architecture and vernacular built heritage comprises a complex set of issues that range from fundamental problematic recognition to anthropological and cultural studies and,more recently,to technological and experimental *** paper addresses the development of the feld,following the milestones of the international literature and pursuing a refective-theory approach within a historical *** aims to explore the main contributions that have enhanced vernacular heritage and earthen architecture as specifc domains,from pioneering public awareness essays to institutional expertise ***,in addition to the literature review process,this paper considers the recent corpus of recommendations from conservation management reference institutions,the updating of the operative problematic of earthen vernacular built heritage,and the relevance of local community involvement in facing increasing challenges.

关键词： Vernacular heritage Earthen architecture Conservation

Motor Response of Mirror Therapy after Reconstruction of Extensor Mechanism Caused by Glass Cut Injury

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Case Reports in Clinical Medicine 2022年第8期11卷 273-279页

作者： Maha Farhina Plastic Surgery Department Dr. Ruth K. M Pfau Civil Hospital Karachi Karachi Pakistan

Treatment by mirror therapy (MT) restores motion in injured limbs without invasive procedures. This process is widely accepted for rehabilitating patients with phantom limb pain, stroke victims, or patients who need therapy after nerve damage. The procedure is specifically useful in restoring motion to the hand after surgical repairs to the extensor muscle and tendons. Mirror therapy rewires the brain by making the restored limb remember hand motions by observing the motions of a normal hand. The concept of a mirror image is that the movement of the uninjured arm forms the illusion of the same movement in the affected arm. Efforts to repeat hand movements elicit the same reaction in the affected hand in what is referred to as Hebbian learning. This case study evaluated MT’s effectiveness in motion restoration after a glass injury. This case study showed restoration of normal hand motions in a patient following surgery to repair a glass cut to the arm. Surgery repaired the lacerated extensor tendon and radial nerve. The muscle belly was repaired, and a graft fixed the nerve gap. Once the arm healed, the patient underwent rehabilitation in mirror therapy to restore normal function in his hand. After conducting mirror therapy, the pain was eliminated, and the patient restored normal function of moving the hand and finger extension. In addition, the therapy could be conducted at home without needing a professional. The effectiveness of mirror therapy was seen in the functional restoration of hand and finger movement. The process is also less complicated as it can be performed at home.

关键词： Mirror Therapy Glass Cut Injury Neuro-Musculoskeletal Extensor Tendon Injury Extensor Muscles

Berrettini Anastomosis Iatrogenic Injury Relating to Carpel Tunnel Syndrome Case Study

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International Journal of Clinical Medicine 2022年第11期13卷 494-500页

作者： Maha Farhina Plastic Surgery Department Dr. Ruth K.M Pfau Civil Hospital Karachi Karachi Pakistan

Some diseases require medical mitigation following the diagnosis, and sometimes the situation means that the patient has to undertake strong medication. However, this medicine is designed pharmacodynamically to interact with other organs before they reach the intended organ. Some mitigation imposes challenges on the involved organ. While the main organ will be healed, some drugs’ footprints will be left out to other organs. These are called iatrogenic injuries. In the case of anastomosis, it requires the alteration of surgical methods or the origin of iatrogenic injury in the arm;thus, the knowledge of anastomosis is very important to the field of medical practice. In addition, carpal tunnel release becomes vital in curing carpal tunnel syndrome. The paper focuses on a case of a 42-year-old woman found to have a complication to her middle finger. This case becomes important for studying Berrettini anastomosis iatrogenic injury, which relates to carpal tunnel syndrome. The Berrettini branch is a complication that facilitates communication between the superficial ulnar and median nerve. The analysis also provides the electro-diagnostic evidence of Berrettini anastomosis on how it can give a position three-finger and thus contribute to a force explanation of the median neuropraxia. Lastly, the paper provides information on the implication of the Berrettini branch for surgical use. The implication of pictures in the digital era can be used to analyze the varied connection and length between the median nerve and ulnar section. It’s easy to locate the position of high risk of iatrogenic injury in the palm due to the consistent location of Berrettini communication branches. The precaution is that operations have to be conducted moderately for a clear vision as the carefully mitigated practice ensures protection from the destruction and injury of the surrounding structures.

关键词： Iatrogenic Injury Berrettini Anastomosis (BA) SNAP-Sensory Nerve Action Potential CTS-Carpal Tunnel Syndrome Median Nerve Ulnar Nerve

The Case Report on the Effectiveness of Infrared Light on Acid Burn Victim

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Case Reports in Clinical Medicine 2022年第12期11卷 527-533页

作者： Maha Farhina Plastic Surgery Department Dr. Ruth K. M. Pau Civil Hospital Karachi Pakistan

Burn injuries are common in Pakistan. However, most people are reluctant to have infrared treatment for their wounds. This study investigated the efficacy of infrared treatment on burn wounds. The procedure involved a burn victim who had sustained acid burns to the neck and face. Nectrotomy was done to remove dead tissue from the burn wound, and after treatment, Low-Level Light was used to reduce pain and enhance healing in the patient. Initial results showed mild hypertrophic scars, no stiffness. Post-operation therapy included physical training regimens and massage. The patient showed satisfactory results and felt less tightness in neck movements by the following treatment. The article concludes by showing that infrared light promoted repair and regrowth at a cellular level.

关键词： Infrared Therapy Acid Burns Photobiomodulation Low Light Laser Therapy Red Light

Frequency of Musculoskeletal Pain among Surgeons in Karachi

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Case Reports in Clinical Medicine 2022年第8期11卷 297-305页

作者： Maha Farhina Plastic Surgery Department Dr. Ruth K.M Pfau Civil Hospital Karachi Karachi Pakistan

To determine the dynamic physical strain exerted on an individual during work, an evaluation of all physical performances is necessary. For surgeons, sustaining healthy ergonomics is essential, particularly for cases requiring long standing hours. Indeed, the absence of proper ergonomics may lead to multiple musculoskeletal issues. Failure to check these problems may lead to future degenerative changes and eventually career-ending damages. Pain among surgeons is expected since they spend many hours caring for patients that they ignore their health, standing for prolonged hours. Lack of attention to work ergonomics often leads to work-related muscle pain and increased risk of problems.

关键词： Musculoskeletal Pain Ergonomics Orthopedic Surgeons Karachi