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Neuropeptide Y receptor Y8b(npy8br)regulates feeding and digestion in Japanese medaka(Oryzias latipes)larvae:evidence from gene knockout

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2024年第7期25卷 605-616页

作者： Xiaodan JIA Ke LU Xufang LIANG College of fisheries Chinese Perch Research CenterHuazhong Agricultural UniversityWuhan 430070China Engineering research center of Green development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt Ministry of EducationWuhan 430070China

Neuropeptide Y receptor Y8(NPY8R)is a fish-specific receptor with two subtypes,NPY8AR and *** in expression levels during physiological processes or in vivo regulation after ventricular injection suggest that NPY8BR plays an important role in feeding regulation;this has been found in only a few fish,at *** order to better understand the physiological function of npy8br,especially in digestion,we used clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)technology to generate npy8br-/-japanese medaka(Oryzias latipes).We found that the deletion of npy8br in medaka larvae affected their feeding and digestion ability,ultimately affecting their ***,npy8br deficiency in medaka larvae resulted in decreased feed intake and decreased expression levels of orexigenic genes(npy and agrp).npy8br-/-medaka larvae fed for 10 d(10th day of feeding)still had incompletely digested brine shrimp(Artemia nauplii)in the digestive tract 8 h after feeding,the messenger RNA(mRNA)expression levels of digestion-related genes(amy,lpl,ctra,and ctrb)were significantly decreased,and the activity of amylase,trypsin,and lipase also significantly *** deletion of npy8br in medaka larvae inhibited the growth and significantly decreased the expression of growth-related genes(gh and igf1).Hematoxylin and eosin(H&E)sections of intestinal tissue showed that npy8br-/-medaka larvae had damaged intestine,thinned intestinal wall,and shortened intestinal *** far,this is the first npy8br gene knockout model established in fish and the first demonstration that npy8br plays an important role in digestion.

关键词： Neuropeptide Y receptor Y8b(npy8br) Japanese medaka(Oryzias latipes) Knockout Feeding Digestion

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Metabolomic-based analysis reveals bile acid-mediated ovarian failure induced by low temperature in zebrafish

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Zoological research 2024年第4期45卷 791-804页

作者： Wen-Hao Li Zhi-Qiang Li Meng-Di Bu Jia-Zhen Li Liang-Biao Chen Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of EducationCollege of Fisheries and Life SciencesShanghai Ocean UniversityShanghai 201306China Laboratory for marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdaoShandong 266237China International research center for marine Biosciences Ministry of Science and TechnologyCollege of Fisheries and Life SciencesShanghai Ocean UniversityShanghai 201306China

As ectotherms, fish are highly sensitive to temperature fluctuations, which can profoundly impact their reproductive cycles. In this study, we investigated the fertility and histological characteristics of zebrafish(Danio rerio) ovaries exposed to a temperature gradient ranging from the thermopreferendum temperature of the species,27℃, to lower temperatures of 22℃, 20℃, and 13℃ over a period of two weeks. Comparative metabolomic(six biological replicates for each temperature) and transcriptomic(four biological replicates for each temperature) analyses were conducted under the four temperature conditions. Results indicated that lower temperatures inhibited oocyte development and differential metabolites were involved in steroid hormone production,antioxidant function, and lipid and protein *** reproductive hormones, increased proteolysis,and lipid degradation significantly impeded oocyte development and egg maturation. Notably, a significant increase in bile acid content was noted in the ovaries of the cold-treated fish, indicating that bile acids play a critical role in ovarian failure. Overall, these findings provide valuable insights into the mechanisms governing the reproductive response of fish to cold stress.

关键词： old stress Ovarian failure Metabolome Zebrafish

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Analyses of fin ray types to detect strontium markers in juvenile blunt-snout bream Megalobrama amblycephala

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Ecological Processes 2024年第1期13卷 206-216页

作者： Yahua Zhu Tao Jiang Xiubao Chen Hongbo Liu Quinton Phelps Jian Yang Wuxi fisheries College Nanjing Agricultural University (WFCNAU) Laboratory of Fishery Microchemistry Freshwater Fisheries Research Center Chinese Academy of Fishery Sciences (FFRCCAFS) Department of Biology Missouri State University (DBMSU)

Background Restocking by introducing hatchery-reared fish into wild habitats aids in the restoration of fishery aquatic ecosystems and reefs to increase the abundance of fish resources, restore the ecological balance of water bodies, and enhance ecosystem functioning. Accurately, rapidly, and effectively evaluating the success of restocking using chemical markers（e.g., strontium [Sr]） remains challenging for fisheries management. Consequently, for nonlethal fish sampling, hard tissues, such as fin rays, have received increasing attention as a target for marking method. However, data on the differences in marking different types of fin rays remain limited. Therefore, we exposed juvenile blunt snout bream individuals（Megalobrama amblycephala） to 0（control group） or 800 mg/L of SrCl2·6H2O（marked group） for 5 days and transferred them into normal aerated water for post-immersion culture. We sampled their pectoral, dorsal, ventral, anal, and caudal fin rays. The Sr marks among the fin types were sampled at 0 and 20 days post-immersion and evaluated using an electron probe micro-analyzer（EPMA） for the five-day Sr/Ca ratios, along with line transect and Sr mapping *** Sr marking signatures were observed in all fin types in the marked group, with a success rate of up to 100%. Although marking efficiency varied among the different fin ray types, the highest Sr/Ca ratios were most often detected in the dorsal fin. Cross-sectional Sr concentration maps of all fin rays sampled showed high-Sr domains in the marked group; in contrast, the entire cross-sections of the control group displayed low Sr contents, indicating successful marking *** Fin ray Sr marking is a successful method for juvenile M. amblycephala, with the advantages of nonlethality and negligible sampling injuries, facilitating the rapid and effective evaluation of Sr marking in restocking M. amblycephala.

关键词： Megalobrama amblycephala Juvenile Fin ray Strontium marking Electron probe micro-analyzer

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Spatial-temporal shifting patterns and in situ conservation of spotted seal(Phoca largha)populations in the Yellow Sea ecoregion

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Integrative Zoology 2024年第2期19卷 307-318页

作者： Hongfei ZHUANG Fei SHAO Chao ZHANG Wancai XIA Shouqiang WANG Fangyuan QU Zongling WANG Zhichuang LU Linlin ZHAO Zhaohui ZHANG First Institute of Oceanography Ministry of Natural ResourcesQingdaoChina Department of Natural Resources of Shandong Province Shandong Forestry Protection and Development Service CenterJinanChina National Park(Protected Areas)development center National Forestry and Grassland AdministrationBeijingChina College of Life Science China West Normal UniversityNanchongChina Liaoning Ocean and fisheries Science research Institute DalianChina

Understanding the habitat shifting pattern is a prerequisite for implementing in situ conservation of migratory *** seals(Phoca largha)inhabiting the Yellow Sea ecoregion(YSE)comprise a small population with independent genes and represent a charismatic flagship species in this ***,this population has declined by 80%since the 1940s,and increased support from the countries around the YSE is urgently needed to address the potential local extinction risk.A time-series niche model and life-history weighted systematic conservation planning were designed on the basis of a satellite beacon tracking survey(2010–2020)of the YSE *** results showed clustering and spreading shifting patterns during the breeding and migratory seasons,*** closed-loop migration route formed in the YSE indicated that this population might be geographically isolated from populations in other breeding areas around the *** conservation priority area(CPA),with an area of 19632 km^(2)(3.58%of the total YSE area),was the most effective response to the potential in situ ***,nearly 80%of the CPA was exposed outside the existing marine protected areas(MPAs).Future establishment of MPAs in China should strategically consider the conservation gap identified herein,and it is recommended for Korea’s closed fishing season to be spatially set in the western Korean Peninsula from May to *** study also exemplified that the lack of temporal information would lead to the dislocation of niche modeling for migratory species represented by spotted *** should be paid to protecting small and migratory populations in marine biodiversity conservation planning.

关键词： conservation priority area marine protected area migration seasonal management spotted seal

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Combining genome-wide association study and transcriptome analysis to identify molecular markers and genetic basis of population-asynchronous ovarian development in Coilia nasus

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Zoological research 2024年第3期45卷 491-505页

作者： Yue Yu Shi-Ming Wan Cheng-You Huang Shuang-Meng Zhang Ai-Li Sun Jun-Qi Liu Shun-Yao Li Yong-Fu Zhu Shu-Xin Gu Ze-Xia Gao College of fisheries Hubei Hongshan Laboratory/Key Lab of Freshwater Animal BreedingMinistry of Agriculture and Rural Affairs/Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic BeltMinistry of EducationHuazhong Agricultural UniversityWuhanHubei 430070China Laboratory for marine Biology and Biotechnology Qingdao Marine Science and Technology CenterQingdaoShandong 266237China Wuhan Hengyuxin Technology Breeding Professional Cooperative WuhanHubei 430117China Hubei Provincial Aquatic Science research Institute WuhanHubei 430208China Zhenjiang Jiangzhiyuan Fishery Technology Co. Ltd.ZhenjiangJiangsu 212213China

Coilia nasus,a migratory fish species found in the middle and lower reaches of the Yangtze River and along offshore areas of China,possesses considerable aquacultural and economic ***,the species faces challenges due to significant variation in the gonadal development rate among females,resulting in inconsistent ovarian maturation times at the population level,an extended reproductive period,and limitations on fish growth rate due to ovarian *** the present study,we combined genome-wide association study(GWAS)and comparative transcriptome analysis to investigate the potential single nucleotide polymorphisms(SNPs)and candidate genes associated with population-asynchronous ovarian development in *** of the female population based on whole-genome resequencing yielded 2120695 high-quality SNPs,39 of which were suggestively associated with ovarian *** note,a significant SNP peak on LG21 containing 30 suggestively associated SNPs was identified,with cpne5a determined as the causal gene of the ***,single-marker and haplotype association analyses were performed on cpne5a,revealing four genetic markers(P0.9)significantly associated with the *** transcriptome analysis of precociously and normally maturing individuals screened out 29 and 426 overlapping differentially expressed genes in the brain and ovary,respectively,between individuals of different body *** the GWAS and transcriptome analysis results,this study identified genes and pathways related to hypothalamic-pituitary-gonadal axis hormone secretion,extracellular matrix,angiogenesis,and gap junctions involved in population-asynchronous ovarian *** insights gained from this study provide a basis for a deeper understanding of the molecular mechanisms underlying ovarian development in fish and may facilitate the genetic breeding of *** strains exhibiting population-synchronous ovarian

关键词： Coilia nasus GWAS Transcriptome Ovarian development SNP

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Analysis of quality-related proteins in golden pompano(Trachinotus ovatus)fillets with modified atmosphere packaging under superchilling storage

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Food Science and Human Wellness 2024年第4期13卷 2253-2265页

作者： Chuang Pan Xiaofan Zhang Shengjun Chen Yong Xue Yanyan Wu Yueqi Wang Di Wang South China Sea fisheries research Institute Chinese Academy of Fishery SciencesKey Laboratory of Aquatic Product ProcessingMinistry of Agriculture and Rural AffairsNational R&D Centre for Aquatic Product ProcessingGuangzhou 510300China Co-Innovation center of Jiangsu marine Bio-industry Technology Jiangsu Ocean UniversityLianyungang 222005China Collaborative Innovation center of Seafood Deep Processing Dalian Polytechnic UniversityDalian 116034China Key Laboratory of Efficient Utilization and Processing of marine Fishery Resources of Hainan Province Sanya Tropical Fisheries Research InstituteSanya 572018China College of Food Science and Engineering Ocean University of ChinaQingdao 266003China

Here,we aimed to study the changes in proteome of golden pompano fillets during post-mortem *** mass tags(TMT)-labeled quantitative proteomic strategy was applied to investigate the relationships between protein changes and quality characteristics of modified atmosphere packaging(MAP)fillets during superchilling(-3°C)*** electron microscopy was used to show that the muscle histology microstructure of fillets was damaged to varying degrees,and low-field nuclear magnetic resonance was used to find that the immobilized water and free water in the muscle of fillets changed *** sulfhydryl content,TCA-soluble peptides and Ca2+-ATPase activity also showed that the fillet protein had a deterioration by oxidation and *** Fresh(FS),MAP,and air packaging(AP)groups were *** of 150 proteins were identified as differential abundant proteins(DAPs)in MAP/FS,while 209 DAPs were in AP/FS *** KEGG pathway analysis indicated that most DAPs were involved in binding proteins and protein *** analysis found that 52 DAPs were correlated with quality *** them,8 highly correlated DAPs are expected to be used as potential quality markers for protein oxidation and water-holding *** results provide a further understanding of the muscle deterioration mechanism of packaging golden pompano fillets during superchilling.

关键词： Tandem mass tags(TMT)proteomics Trachinotus ovatus Modified atmosphere packaging Superchilling storage Low-field nuclear magnetic resonance Protein deterioration

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Combined ATAC-seq,RNA-seq,and GWAS analysis reveals glycogen metabolism regulatory network in Jinjiang oyster(Crassostrea ariakensis)

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Zoological research 2024年第1期45卷 201-214页

作者： Biao Wu Xi Chen Jie Hu Zhen-Yuan Wang Yan Wang Da-You Xu Hao-Bing Guo Chang-Wei Shao Li-Qing Zhou Xiu-Jun Sun Tao Yu Xiao-Mei Wang Yan-Xin Zheng Guang-Yi Fan Zhi-Hong Liu State Key Laboratory of Mariculture Biobreeding and Sustainable Goods Yellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdaoShandong 266071China Laboratory for marine fisheries Science and Food Production Processes Laoshan LaboratoryQingdaoShandong 266071China BGI-Qingdao BGI-ShenzhenQingdaoShandong 266426China National Demonstration center for Experimental fisheries Science Education Shanghai Ocean UniversityShanghai 201306China Changdao Enhancement and Experiment Station Chinese Academy of Fishery SciencesYantaiShandong 265800China State Key Laboratory of Agricultural Genomics BGI-ShenzhenShenzhenGuangdong 518083China

Glycogen serves as the principal energy reserve for metabolic processes in aquatic shellfish and substantially contributes to the flavor and quality of *** Jinjiang oyster(Crassostrea ariakensis)is an economically and ecologically important species in *** the present study,RNA sequencing(RNA-seq)and assay for transposase-accessible chromatin using sequencing(ATAC-seq)were performed to investigate gene expression and chromatin accessibility variations in oysters with different glycogen *** identified 9483 differentially expressed genes(DEGs)and 7215 genes with significantly differential chromatin accessibility(DCAGs)were obtained,with an overlap of 2600 genes between ***,a significant proportion of these genes were enriched in pathways related to glycogen metabolism,including“Glycogen metabolic process”and“Starch and sucrose metabolism”.In addition,genome-wide association study(GWAS)identified 526 single nucleotide polymorphism(SNP)loci associated with glycogen *** loci corresponded to 241 genes,63 of which were categorized as both DEGs and *** study enriches basic research data and provides insights into the molecular mechanisms underlying the regulation of glycogen metabolism in ***.

关键词： Crassostrea ariakensis Glycogen Transcriptome ATAC GWAS

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Self-fertilization in a gonochoristic fish yellow catfish

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Aquaculture and fisheries 2024年第2期9卷 234-244页

作者： Yue Yu Ziyi Lu Min Chen Ya Liu Yang Ding Chenhao Hu Zhigang Shen College of fisheries Key Lab of Freshwater Animal BreedingMinistry of Agriculture and Rural AffairsWuhan430070China Key Lab of Agricultural Animal Genetics Breeding and Reproduction of Ministry of EducationWuhan430070China Engineering Technology research center for Fish Breeding and Culture in Hubei Province Wuhan430070China Engineering research center of Green development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt of Ministry of Education Huazhong Agricultural UniversityWuhan430070China Hubei Huangyouyuan Fishery development Limited Company Wuhan430070China

Sex control techniques are in great demand for aquaculture because of the huge benefits of mono-sex *** a previous study on yellow catfish(Tachysurus fulvidraco),intersex individuals were produced by administrating 17α-methyltestosterone(MT)or aromatase inhibitor letrozole(LZ).The ovarian and testicular portions of intersexual gonads developed well and were completely separated,arousing our interest in producing self-fertilizable fish and clone-like fish *** this study,yellow catfish fry were treated with a combination of MT and LZ at a low dose(5 mg kg^(-1)MT,300 mg kg^(-1)LZ)at a low water temperature(27.0℃,mixed sex-producing temperature,LL group)and a high temperature(33.5◦C,male-producing temperature,HL group),and with a high dose(10 mg kg^(-1) MT,600 mg kg^(-1) LZ)at a low water temperature(LH group),during 12-65 days post-hatching,the critical period of sex *** XX and XY intersex were produced with different percentages at the same time through co-treatment with MT and LZ,with or without the help of male-producing *** highest percentage of XY intersex(53%in XY,36%in XX and XY)and XX intersex(47%in XX,25%in XX and XY)was produced in LH and HL groups,*** of gonadal morphology,histology,and sperm dynamic analysis indicate that some XX and XY intersex individuals were capable of self-fertilization through artificial *** reproductive performance of these intersex individuals was then investigated by injecting spawning agents,dissecting gonads,and artificially fertilizing eggs with their own *** XX,XY,and YY genotypic individuals were produced by the self-fertilization of XY intersex *** present study successfully produced high percentages of XX and XY intersex and conducted self-fertilization by mating the eggs and sperm of a single intersex ***-fertilization in fish will show great potential in aquaculture genetics and breeding,for example,generating high

关键词： Sex differentiation Self-fertilization Sex reversal Mono-sex Clone

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Insights into microbiota community dynamics and flavor development mechanism during golden pomfret(Trachinotus ovatus)fermentation based on single-molecule real-time sequencing and molecular networking analysis

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Food Science and Human Wellness 2024年第1期13卷 101-114页

作者： Yueqi Wang Qian Chen Huan Xiang Dongxiao Sun-Waterhouse Shengjun Chen Yongqiang Zhao Laihao Li Yanyan Wu Key Laboratory of Aquatic Product Processing Ministry of Agriculture and Rural Affairs of The People’s Republic of ChinaNational R&D Center for Aquatic Product ProcessingSouth China Fisheries Research InstituteChinese Academy of Fishery SciencesGuangzhou 510300China Co-Innovation center of Jiangsu marine Bio-industry Technology Jiangsu Ocean UniversityLianyungang 222005China School of Chemical Sciences The University of AucklandAuckland 92019New Zealand Collaborative Innovation center of Seafood Deep Processing Dalian Polytechnic UniversityDalian 116034China

Popular fermented golden pomfret(Trachinotus ovatus)is prepared via spontaneous fermentation;however,the mechanisms underlying the regulation of its flavor development remain *** study shows the roles of the complex microbiota and the dynamic changes in microbial community and flavor compounds during fish ***-molecule real-time sequencing and molecular networking analysis revealed the correlations among different microbial genera and the relationships between microbial taxa and volatile *** underlying flavor development were also elucidated via KEGG based functional ***,Shewanella,and Staphylococcus were the dominant microbial ***-nine volatile compounds were detected in the fermented fish samples,with thirteen identified as characteristic volatile compounds(ROAV>1).Volatile profiles resulted from the interactions among the microorganisms and derived enzymes,with the main metabolic pathways being amino acid biosynthesis/metabolism,carbon metabolism,and glycolysis/*** study demonstrated the approaches for distinguishing key microbiota associated with volatile compounds and monitoring the industrial production of high-quality fermented fish products.

关键词： Fermented golden pomfret Microbiota community Volatile compound Co-occurrence network Metabolic pathway

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Application of Bionic Technology in marine Cruise Equipment:research Progress and development Trends

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Journal of Bionic Engineering 2024年第3期21卷 1117-1155页

作者： Kunhui Luo Lan Yan Zhiyi Zhu Zhongqiu Wang Hongru Wang Feng Jiang School of Electromechanical and Automation Huaqiao UniversityXiamen36102China National Local Joint Engineering research center for Intelligent Manufacturing Technology of Brittle Material Products Xiamen361021China Institute of Oceanographic Instrumentation Shandong Academy of SciencesQilu University of Technology(Shandong Academy of Sciences)Qingdao266100China Shandong Provincial Key Laboratory of marine Monitoring Instrument Equipment Technology Qingdao266100China National Engineering and Technological research center of marine Monitoring Equipment Qingdao266100China Institute of Manufacturing Engineering Huaqiao UniversityXiamen361021China

This article provides an overview of the application of bionic technology in marine cruising equipment,discussing its research progress and future development *** cruising is a crucial means of gaining insights into the marine environment and conducting scientific ***,conventional marine cruising equipment faces numerous challenges when dealing with complex and ever-changing marine *** technology,as a means of drawing inspiration from the structure and functions of living organisms,offers new approaches and methods to address the challenges faced by marine cruising equipment and has found widespread *** article primarily focuses on the applications and historical developments of bionic technology in propulsion methods,drag reduction,and surface *** summarizes the design principles,manufacturing techniques,and optimization methods for marine biomimetic cruising ***,this paper analyzes the achievements,challenges,and future directions of bionic technology in marine cruising *** application of bionic technology in marine cruising equipment holds vast potential for development,enabling us to better confront the challenges of marine exploration and research by drawing wisdom from nature and driving advancements in marine science.

关键词： marine cruising equipment Bionic technology Propulsion methods Drag reduction Surface antifouling

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