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Electroformation and collection of giant liposomes on an integrated microchip

Chinese Chemical Letters 2019年第2期30卷 353-358页

作者： Zhenyu Wang Chaoxiong Wu Ting Fan Xianwei Han Qiong Wang Jincan Lei Jun Yang State Key Laboratory of Ultrasound engineering in Medicine Co-Founded by chongqing ξ the Ministry of Science ξ technology College of Biomedical Engineering Chongqing Medical University Key Laboratory of Biorheological Science and technology (chongqing University) Ministry of Education and Key Lab of Visual Damage and Regeneration ξ Restoration of ChongqingBioengineering College Chongqing University chongqing engineering and technology research center of intelligent rehabilitation and eldercare Chongqing City Management College

Giant liposome is an important lipid structure widely used in biological and medical fields. In its main preparation method, electroformation, many influencing factors must be optimized for good effect. How to collect the desired giant liposomes is another major issue. In this work, a microchip with a reactor chamber array was used to study the influences of multiple parameters, and a suitable condition could be achieved rapidly and efficiently. A tailor-made collection chamber was also integrated on the chip. Based on the multifactor and multilevel orthogonal experiment, optimal conditions of the lipid solution, buffer solution, and electric signal were achieved with high efficiency. More than one thousand giant liposomes could be formed in each microscale reactor chamber, and most of them were unilamellar. The on-chip collection ratio of giant liposome carriers could also approximate to 40%.

关键词： Giant liposome Microchip ITO Electroformation Collection

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