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Effect of Bacillus baekryungensis YD13 Supplemented in Diets on Growth Performance and Immune Response of Sea Cucumber(Apostichopus japonicus)

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Journal of Ocean University of china 2014年第5期13卷 805-810页

作者： YAN Fajun TIAN Xiangli DONG Shuanglin The Key laboratory of mariculture of Ministry of Education Fisheries College Ocean University of China

The effect of a potential probiotic on the growth performance and immune response of sea cucumber(Apostichopus japonicus) was investigated. Bacillus baekryungensis YD13 isolated from sea cucumber culturing ponds was added to sea cucumber basal feed as a probiotic in different doses(0, the control; 1×104(YD134), 1×106(YD136) and 1×108(YD138) CFU g-1 of diet), and administered orally to A. japonicus(initial mean wet weight 5.44 g ± 0.17 g). The sea cucumbers were fed in 20 aquaria, 5 each treatment, for 60 d. At the end of growth trial, 20 sea cucumbers from each treatment were challenged with Vibrio splendidus. A. japonicus in YD134 and YD136 exhibited significantly better growth performance than control(P < 0.05). Five non-specific immune parameters including lysozyme, acid phosphatase, alkaline phosphatase, superoxide dismutase and catalase in coelomic fluid were measured to evaluate the immune response of A. japonicus to the probiotics. Results showed that all parameters were significantly improved when YD11 was supplemented in the dose of 1×106 CFU g-1(P < 0.05). The cumulative incidence and mortality after the Vibrio splendidus challenge decreased significantly in sea cucumbers of YD136. Accordingly, 1×106 CFU g-1 of YD13 in diet was recommended for the growth promotion and immune enhancement of A. japonicus.

关键词： Bacillus baekryungensis probiotics sea cucumber Apostichopus japonicus growth performance immune response

Change of Digestive Physiology in Sea Cucumber Apostichopus japonicus(Selenka) Induced by Corn Kernels Meal and Soybean Meal in Diets

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Journal of Ocean University of china 2016年第4期15卷 697-703页

作者： YU Haibo GAO Qinfeng DONG Shuanglin HOU Yiran WEN Bin Key laboratory of mariculture of Ministry of Education Ocean University of China College of Animal Science and technology Northwest A&F University

The present study was conducted to determine the change of digestive physiology in sea cucumber Apostichopus japonicus(Selenka) induced by corn kernels meal and soybean meal in diets. Four experimental diets were tested, in which Sargassum thunbergii was proportionally replaced by the mixture of corn kernels meal and soybean meal. The growth performance, body composition and intestinal digestive enzyme activities in A. japonicus fed these 4 diets were examined. Results showed that the sea cucumber exhibited the maximum growth rate when 20% of S. thunbergii in the diet was replaced by corn kernels meal and soybean meal, while 40% of S. thunbergii in the diet can be replaced by the mixture of corn kernels meal and soybean meal without adversely affecting growth performance of A. japonicus. The activities of intestinal trypsin and amylase in A. japonicus can be significantly altered by corn kernels meal and soybean meal in diets. Trypsin activity in the intestine of A. japonicus significantly increased in the treatment groups compared to the control, suggesting that the supplement of corn kernels meal and soybean meal in the diets might increase the intestinal trypsin activity of A. japonicus. However, amylase activity in the intestine of A. japonicus remarkably decreased with the increasing replacement level of S. thunbergii by the mixture of corn kernels meal and soybean meal, suggesting that supplement of corn kernels meal and soybean meal in the diets might decrease the intestinal amylase activity of A. japonicus.

关键词： soybean cucumber amylase digestive intestine replaced Selenka trypsin Sargassum altered

Evolutionary dynamics of the Wnt gene family:implications for lophotrochozoans

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Journal of Oceanology and Limnology 2018年第5期36卷 1720-1730页

作者： LIU Jun XU Fei JI Peng LI Li ZHANG Guofan Key laboratory of Experimental Marine Biology Institute of OceanologyChinese Academy of SciencesQingdao 266071China University of ChineseAcademy of Sciences Beijing 100049China laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao 266000China National ＆ Local joint Engineering laboratory of Ecological mariculture Institute of OceanologyChinese Academy of SciencesQingdao 266071China laboratory for Marine Fisheries and Aquaculture Qingdao National Laboratory for Marine Science and TechnologyQingdao 266000China

Genes encoding Wnt ligands, which have important roles in cell communication and organ development, are restricted to multicellular animals. We systematically studied W nt genes from eumetazoan genomes, with emphasis on the poorly studied superphylum Lophotrochozoa(four annelids, seven mollusks, eight platyhelminths, one bdelloid rotifer, and one brachiopod species). Between 3 and 39 W nt loci were identified in each genome, and the protostome-specific loss of Wnt3 genes was confirmed. We identified gastropod-specific loss of Wnt8, refining the previously proposed mollusk-specific loss. Some duplicated Wnt genes belonging to a same subfamily or closely related subfamilies showed tandem distribution in the lophotrochozoan genomes, indicating tandem duplication events during Wnt family evolution. Members of the conserved Wnt10-Wnt6-Wnt1-Wnt9 cluster showed highly correlated expression patterns over time in two assayed lophotrochozoans, the oyster C rassostrea gigas and the brachiopod L ingula anatina, reflecting the possible similar function of the clustered W nt genes.

关键词： phylogeny gene cluster time-course expression tissue distribution

Peptidoglycan-inspired autonomous ultrafast self-healing bio-friendly elastomers for bio-integrated electronics

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National Science Review 2021年第5期8卷 130-139页

作者： Luzhi Zhang Jiahui Liang Chenyu Jiang Zenghe Liu Lijie Sun Shuo Chen Huixia Xuan Dong Lei Qingbao Guan Xiaofeng Ye Zhengwei You State Key laboratory for Modification of Chemical Fibers and Polymer Materials Shanghai Belt and Road Joint Laboratory of Advanced Fiber and Low-dimension MaterialsCollege of Materials Science and EngineeringDonghua University Department of Cardiac Surgery Ruijin HospitalShanghai Jiao Tong University School of Medicine

Elastomers are essential for stretchable electronics, which have become more and more important in bio-integrated devices. To ensure high compliance with the application environment, elastomers are expected to resist, and even self-repair, mechanical damage, while being friendly to the human ***, inspired by peptidoglycan, we designed the first room-temperature autonomous self-healing biodegradable and biocompatible elastomers, poly(sebacoyl 1,6-hexamethylenedicarbamate diglyceride)(PSe HCD) elastomers. The unique structure including alternating ester-urethane moieties and bionic hybrid crosslinking endowed PSeHCD elastomers superior properties including ultrafast self-healing,tunable biomimetic mechanical properties, facile reprocessability, as well as good biocompatibility and biodegradability. The potential of the PSeHCD elastomers was demonstrated as a super-fast self-healing stretchable conductor(21 s) and motion sensor(2 min). This work provides a new design and synthetic principle of elastomers for applications in bio-integrated electronics.

关键词： biomimetic elastomers ultrafast self-healing bio-integrated electronics stretchable conductor

Relationships of growth and mortality to enzymatic activity,and the relative mRNA expression of cultured scallops Patinopecten yessoensis in the Yellow Sea, china

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Journal of Oceanology and Limnology 2019年第4期37卷 1409-1422页

作者： LIU Chao WU Fucun QUE Huayong ZHANG Guofan Key laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China University of Chinese Academy of Sciences Beijing 100049 China laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology Qingdao 266071 China National & Local joint Engineering laboratory of Ecological mariculture Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China

The bottom aquaculture of yesso scallop (Patinopecten yessoensis) has developed rapidly in the north of Yellow Sea, china, but not without accompanying productivity problems. We, therefore, conducted field surveys to investigate factors related to growth and mortality rates of bottom-cultured scallops after release. Specifically, we focused on the effects of total antioxidant capacity (TAOC) and three key enzymes involved in antioxidant and metabolic function:glutamic pyruvic transaminase (GPT), lactic dehydrogenase (LDH), and superoxide dismutase (SOD). Across summer and winter, we also measured the relative mRNA expression of GPT and SOD to understand their seasonal variation in the scallops, along with how such variation correlated to growth and mortality. Results show that bottom cultured scallops experienced mass mortality during the first six months post-release. During winter (December), scallops grew more rapidly and suffered less mortality than in summer (August). The observed lower performance probably resulted from less advantageous environmental factors during the summer seasons, such as high temperature and low dissolved oxygen. These environmental stressors enhance protein consumption while decreasing energetic resources in scallops. Furthermore, scallops in summer exhibited high antioxidant levels that probably competed for energy with process integral to growth and survival. These negative factors combined to elevate mortality rates. In conclusion, we provided evidence suggesting correlations between metabolic/antioxidant activity and growth and mortality of bottom-cultured yesso scallops. These correlations implied us an accurate method to estimate the performance of bottom culture system. Suggestions about innovative aquaculture techniques were also discussed in the study. Our results might provide a possible guideline to the improvement of bottom culture techniques for this commercially valuable seafood species.

关键词： yesso scallop (Patinopecten yessoensis) bottom culture growth and mortality rate antioxidant and metabolic activity relative mRNA expression

Association of triacylglyceride content and transcript abundance of genes involving in lipid synthesis of nitrogen deficient Phaeodactylum tricornutum

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Chinese Journal of Oceanology and Limnology 2014年第2期32卷 397-402页

作者：张琳韩吉昌杨官品朱葆华潘克厚 laboratory of Applied Microalgae Biology Key Laboratory of Mariculture of Ministry of Education of ChinaOcean University of China College of Marine Life Sciences Ocean University of China

Phaeodactylum tricornutum is a diatom that is rich in lipids. Recently, it has received much attention as a feedstock for biodiesel production. Nitrogen deficiency is widely known to increase the content of neutral lipids （mainly triacylglycerides, or TAGs） of microalgae, including P. tricornutum, but the mechanism is unclear. In this study, we deciphered the correlations between TAG content and nine key enzymatic genes involved in lipid synthesis in P. tricornuturn. After being cultured under nitrogen-free conditions for 0, 4, 24, 48, 72, 120, and 168 h, the TAG contents ofP. tricornutum cells were assayed and the transcript abundances of the target genes were monitored by quantitative real-time PCR. The results show that the abundances of four target gene transcripts （LACS3, G3PDH2, G3PDH3, and G3PDH5） were positively correlated with TAG content, indicating that these genes may be involved in TAG synthesis in P. tricornutum. The findings improve our understanding of the metabolic network and regulation of lipid synthesis and will guide the future genetic improvement of the TAG content ofP. tricornutum.

关键词： Phaeodactylum tricornutum TAG content transcript abundance

Effect of Temperature on the Carbon, Nitrogen, and Phosphorus Nutrient Budgets of Steelhead Trout (Oncorhynchus mykiss) with Different Sizes

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Journal of Ocean University of china 2022年第5期21卷 1283-1294页

作者： ZHANG Gong GAO Qinfeng ZHOU Yangen DONG Shuanglin WANG Meijun GAO Yonggang Key laboratory of mariculture Ministry of EducationOcean University of ChinaQingdao 266003China Function laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and TechnologyQingdao 266100China Fishery technology Promotion Station of Rongcheng City Weihai 264300China

This study was performed to evaluate the effect of temperature on the carbon(C),nitrogen(N),and phosphorus(P)nu-trient budgets of adult steelhead trout(Oncorhynchus mykiss)with different body sizes.A complete two-factor experiment was con-ducted at four temperatures,i.e.,9(T9),13(T13),16(T1℃℃℃6),and 19(T19);and three body sizes,℃i.e.,0.65 kg±0.02 kg(S1),1.22 kg±0.06 kg(S2),and 2.05 kg±0.09 kg(S3).The following properties of steelhead trout were determined:C,N,and P nutrient budgets;feed intake rate;fecal production rate;oxygen consumption rate;ammonia excretion rate;phosphorus excretion rate;scope of the growth of C,N,and P;and absorption efficiency of C,N,and *** showed no significant interaction between temperature and body size in terms of the nutrient budgets of steelhead ***,temperature and body size played important separate roles in the nutrient budgets except for the absorption efficiency of *** feed intake rate,fecal production rate,ammonia excretion rate,phosphorus excretion rate,and scope of the growth of C,N,and P were the highest in the T16 *** absorption effi-ciency of C and P decreased as temperature *** multiple regression results indicated that 15 was the approximate℃optimal temperature for the feeding and growth of steelhead *** feed intake rate,fecal production rate,oxygen consumption rate,ammonia excretion rate,phosphorus excretion rate,and scope of the growth of C,N,and P per unit of the body weight of fish decreased as body size *** absorption efficiency of C increased as body weight increased,and the absorption efficiencies of P in the S2 and S3 treatments were significantly higher than those in the S1 *** the culture of steelhead trout,the daily ratio should be adjusted with changes in the temperature and body size of ***,managers should focus on the risk of hypoxia at high water temperatures.

关键词： steelhead trout temperature body size nutrient budget

Dietary Supplementation of β-Carotene Reveals miRNAs Involved in the Regulation of Carotenoid Metabolism in Crassostrea gigas

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Journal of Ocean University of china 2024年第4期23卷 1045-1053页

作者： SUN Dongfang WAN Sai YU Hong KONG Lingfeng LI Qi Key laboratory of mariculture Ministry of EducationOcean University of ChinaQingdao 266003China laboratory for Marine Fisheries Science and Food Production Processes Laoshan LaboratoryQingdao 266237China

Carotenoids play crucial physiological roles in animals.A comprehensive investigation into the mechanism of carotenoid metabolism in oysters will establish a theoretical foundation for further development of its carotenoid-rich ***,the information on the function of miRNA in β-carotene metabolism in oysters is *** elucidate the mechanisms underlying miRNA regulation of carotenoid metabolism in oysters,we compared the expressions of miRNA in digestive gland tissues of Pacific oyster(Crassostrea gigas)fed with aβ-carotene supplemented diet and a normal diet,respectively.A total of 690 candidate miRNAs in the Pacific oyster digestive gland tissues were identified,including 590 known miRNAs and 111 unknown *** differentially expressed miRNAs were obtained in the carotenoid-fed and normal groups,associated to 137 differentially expressed target ***,the GO enrichment analysis revealed that the differentially expressed target genes were mainly involved in transmembrane transport *** enrichment showed that the differentially expressed target genes were involved in ABC *** of the mRNA-miRNA network revealed that novel0025 played a central role in carotenoid metabolism,and it was negatively correlated with the expression of 46 *** addition,down-regulated expression of novel0025 upregulated the expression of the lipoprotein gene LOC105342186,suggesting a potential regulatory role in carotenoid *** results provide useful information for elucidating the miRNA regulation mechanism during carotenoids metabolism in the Pacific oyster.

关键词： Crassostrea gigas β-carotene microRNA carotenoid metabolism

De novo characterization of Larimichthys crocea transcrip- tome for growth-/immune-related gene identification and massive microsatellite (SSR) marker development

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Chinese Journal of Oceanology and Limnology 2017年第2期35卷 225-234页

作者：韩兆方肖世俊刘贤德刘洋李佳凯谢仰杰王志勇 Key laboratory of Healthy mariculture for the East china Sea Ministry of AgricultureChina Fisheries College Jimei UniversityXiamen 361021China

The large yellow croaker, Larimichthys crocea is an important marine fish in china with a high economic value. In the last decade, the stock conservation and aquaculture industry of this species have been facing severe challenges because of wild population collapse and degeneration of important economic traits. However, genes contributing to growth and immunity in L. crocea have not been thoroughly analyzed, and available molecular markers are still not sufficient for genetic resource management and molecular selection. In this work, we sequenced the transcriptome in L. eroeea liver tissue with a Roche 454 sequencing platform and assembled the transcriptome into 93 801 transcripts. Of them, 38 856 transcripts were successfully annotated in nt, nr, Swiss-Prot, InterPro, COG, GO and KEGG databases. Based on the annotation information, 3 165 unigenes related to growth and immunity were identified. Additionally, a total of 6 391 simple sequence repeats （SSRs） were identified from the transcriptome, among which 4 498 SSRs had enough flanking regions to design primers for polymerase chain reactions （PCR）. To access the polymorphism of these markers, 30 primer pairs were randomly selected for PCR amplification and validation in 30 individuals, and 12 primer pairs （40.0%） exhibited obvious length polymorphisms. This work applied RNA-Seq to assemble and analyze a live transeriptome in L. crocea. With gene annotation and sequence information, genes related to growth and immunity were identified and massive SSR markers were developed, providing valuable genetic resources for future gene functional analysis and selective breeding ofL. eroeea.

关键词： Larimichthys crocea transcriptome functional genes SSR markers