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The Breeding of a Pigment Mutant Strain of Steroid Hydroxylation Aspergillus Flavus by Low Energy Ion Implantation

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Plasma science and technology 2009年第1期11卷 122-126页

作者：叶辉马金铭冯春程郢朱苏文程备久 Anhui agricultural University University of science and technology of china

In the process of the fermentation of steroid C11α-hydroxylgenation strain Aspergillus flavus AF-ANo208, a red pigment is derived, which will affect the isolation and purification of the target product. Low energy ion beam implantation is a new tool for breeding excellent mutant strains. In this study, the ion beam implantation experiments were performed by infusing two different ions： argon ion （Ar^＋） and nitrogen ion （N^＋）. The results showed that the optimal ion implantation was N^＋ with an optimum dose of 2.08- 1015 ions/cm^2, with which the mutant strain AF-ANm16 that produced no red pigment was obtained. The strain had high genetic stability and kept the strong capacity of C11α-hydroxylgenation, which could be utilized in industrial fermentation. The differences between the original strain and the mutant strain at a molecular level were analyzed by randomly amplified polymorphic DNA （RAPD）. The results indicated that the frequency of variation was 7.00%, which would establish the basis of application investigation into the breeding of pigment mutant strains by low energy ion implantation.

关键词： ion implantation aspergillus flavus C11-αhydroxylgenation pigment RAPD

Research fronts of agriculture in 2023

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Frontiers of agricultural science and Engineering 2024年第2期11卷 347-354页

作者： Jianxiang XU Yunzhou LI Jie ZHAO Liang SHI Yinkun YAO Jingyue TANG science and technology Journal Center Higher Education PressBeijing 100029China FASE Editorial Office of Institute for science and technology Development China Agricultural UniversityBeijing 100193China

At the end of 2023, Chinese Academy of sciences(CAS) and Chinese Academy of Engineering(CAE) successively announced “2023 Research Fronts”^[1] and “2023 Engineering Fronts”^([2,3]), respectively. Among them, agric... 详细信息

关键词： occupy successively agriculture

Neuroglobin mutation associated with hypoxia adaptation in Tibet chicken

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Progress in Natural science:Materials International 2007年第12期17卷 1419-1424页

作者： Zhang Lifan1, Liu Chong2, Lian Linsheng3, Zhao Chunjiang1, Bao Haigang1 and Wu Changxin1(1. College of Animal science and technology, china agricultural University, Beijing 100094, china 2. College of Biological science, china agricultural University, Beijing 100094, china 3. College of Animal science and technology, Yunnan agricultural University, Kunming 650201, china) College of Animal science and technology China Agricultural University Beijing 100094 China College of Biological science China Agricultural University Beijing 100094 China College of Animal science and technology Yunnan Agricultural University Kunming 650201 China

Globin protein family plays an important role in storing and transporting oxygen. As a newly reported globin, the revealed function of neuroglobin includes binding and storing oxygen as well as facilitating the utilization of oxygen in neuronal cells. In the present study, mutations in exons of chicken Ngb gene were identified with the method of sequencing and created restriction site PCR in Tibet chicken and other four lowland chicken breeds. The mutations of Lys-2224(E4)-Asn and Ser-2279(E4)-Gly were identified in exon 4 of the gene. The Lys-2224(E4)-Asn mutation existed only in Tibet chicken and the mutation frequencies increased with increasing altitude. Analysis of the haplotype and diplotype of the two mutations in Tibet chicken populations of different altitudes showed that the frequencies of TG haplotype and TTGG diplotype also increased with increasing altitude, while the reverse tendency was found on GGAA diplotype. Under the hypoxic simulation incubation, the main haplotype was TG in living embryos and GA in dead embryos. The results showed that the Lys-2224(E4)-Asn mutation may be a specific mutation associated with hypoxia adaptation in Tibet chicken.

关键词： Tibet chicken neuroglobin hypoxia adaptation Ngb gene mutation.

Preparation of Y-H_3PW_(12)O_(40)/TiO_2 and La-H_3PW_(12)O_(40)/TiO_2 by hydrothermal method and its photocatalytic activity for dinitrotoluene decomposition

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Journal of Rare Earths 2014年第1期32卷 17-22页

作者：刘霞尚海茹冯长根 College of science China Agricultural University State Key Laboratory of Explosion science and technology Beijing Institute of Technology

A series of mesoporous materials （H3PW12O40/TiO2, Y-H3PW12O40/TiO2 and La-H3PW12O40/TiO2） were prepared by a modified sol-gel-hydrothermal route, which realized the load and modification of H3PW12O40 at the same time. The prepared samples were characterized by X-ray diffraction （XRD）, Fourier transform infrared spectroscopy （FT-IR）, nitrogen adsorption-desorption analysis, ultraviolet-visible absorption spectrum （UV-Vis） and scanning electron microscopy （SEM）. The XRD and FT-IR results displayed that the catalysts had uniform anatase phase and the primary Keggin structure of H3PW12O40 remain intact. Nitrogen ad-sorption-desorption analysis suggested that suitable doping of rare earth elements could increase the specific surface area from 177.9 m2/g （H3PW12O40/TiO2） to 229.5 （1 wt.%Y-H3PW12O40/TiO2） or 236.1 m2/g （1 wt.%La-H3PW12O40/TiO2）. Results of UV-Vis spectra showed that the band of the 1 wt.%Y-H3PW12O40/TiO2 and 1 wt.%La-H3PW12O40/TiO2 have an obvious redshift compared with the H3PW12O40/TiO2. Additionally, the composites were used as heterogeneous photocatalysts to the degradation of dinitrotoluene （DNT）. It is the first time that polyoxometalate （POM） is applied in the degradation of explosive wastewater.

关键词： hydrothermal POM/TiO2 rare earth elements photocatalytic dinitrotoluene reaction rate

Study on photodegradation of Azo dye by polyoxometalates/polyvinyl alcohol

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Journal of Rare Earths 2009年第5期27卷 717-722页

作者：冯长根卓晓曦刘霞 State Key Laboratory of Explosion science and technology Beijing Institute of Technology College of science China Agricultural University

A series of photocatalysts, K11[Ln(PW11O39)2]/PVA (Ln=La, Ce, Pr, Nd, Sm) were prepared by K11[Ln(PW11O39)2] (Ln=La, Ce, Pr, Nd, Sm) containing five kinds of lanthanides and polyvinyl alcohol as the support. The catalysts obtained were characterized by Fourier transform infrared spectra, UV-vis spectra, powder X-ray diffraction, and scanning electron microscopy, indicating that the structure of K11[Ln(PW11O39)2] and polyvinyl alcohol remained intact, respectively. The photocatalysts exhibited efficient catalytic activity to degrade methyl orange, Congo Red, Ponceau 2R. The maximal degradation conversions of the three kinds of dyes were 99.58%, 47.61%, 72.42%, respectively.

关键词： polyoxometalates polyvinyl alcohol degradation azo dye rare earths

Paternal inheritance of mitochondrial DNA in the sheep (Ovine aries)

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science china(Life sciences) 2001年第3期44卷 321-326页

作者：赵兴波储明星李宁吴常信 Department of Animal science and technology China Agricultural University Beijing China Institute of Animal science Chinese Academy of Agricultural Science Beijing China National Laboratories for Agrobiotechnology China Agricultural University Beijing China

Paternal inheritance of mitochondria DNA in sheep was discovered by examination of 152 sheep from 38 hybrid families for mtDNA D-loop polymorphisms using PCR-RFLP, amplification of repeated sequence somain, and PCR-SSCP of the D-loop 5′ end region of a 253 bp fragment. Our findings have provided the first evidence of paternal inheritance of mtDNA in sheep and possible mechanisms of paternal inheritance were discussed.

关键词： sheep mitochondria DNA paternal inheritance.

Metabolome and RNA-seq reveal discrepant metabolism and secretory metabolism profile in skeletal muscle between obese and lean pigs at different ages

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science china Life sciences 2025年

作者： Liu Guo Junfei Xu Wenyue Zhou Sisi Chen Hanjing Shi Mengmeng Han Zekun Yang Yehui Duan Weijun Pang Yulong Yin Fengna Li Laboratory of Animal Nutritional Physiology and Metabolic Process Key Laboratory of Agro-ecological Processes in Subtropical Region Institute of Subtropical Agriculture Chinese Academy of Sciences University of Chinese Academy of sciences Laboratory of Animal Fat Deposition and Muscle Development College of Animal Science and Technology Northwest A&F University College of Advanced agricultural sciences University of Chinese Academy of Sciences

Metabolites and metabolism-related gene expression profiles in skeletal muscle change dramatically under obesity, aging and metabolic disease. Since obese and lean pigs are ideal models for metabolic research. Here, we compared metabolome and transcriptome of Longissimus dorsi(LD) muscle between Taoyuan black(TB, obese) and Duroc(lean) pigs at different ages. We defined the “window phase” of intramuscular fat(IMF) deposition in TB pig, which has significantly higher IMF than Duroc pig. Our results displayed discrepant lipid composition and different expression genes(DEGs) enriched in lipid metabolism, and both metabolome and transcriptome analyses revealed stronger energy expenditure and more active amino acid and protein metabolism in Duroc pig. 10 up-and 51 down-regulated biomarker metabolites with age-and breed-specificity were identified. Potential secretory metabolites, including organic acid(fumaric acid, succinate,malic acid, and gamma-aminobutyric acid), amino acid(L-lysine, and L-glutamic acid), lipid(2-hydroxyisovaleric acid, and L-carnitine) were demonstrated a significant correlation with IMF deposition. Our research highlights the huge difference of metabolic spectrum in skeletal muscle between obese and lean model and muscle-derived secretory metabolites might act as an ambassador of intercellular communication to regulate systematic metabolism.

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Genome-wide identification and co-expression network analysis of the Os NF-Y gene family in rice

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The Crop Journal 2017年第1期5卷 21-31页

作者： Wenjie Yang Zhanhua Lu Yufei Xiong Jialing Yao College of Life science and technology Huazhong Agricultural UniversityWuhan 430070China College of Plant science and technology Huazhong Agricultural UniversityWuhan 430070China

Nuclear factor Y(NF-Y) is a ubiquitous transcription factor that regulates important physiological and developmental processes. In this study, we identified 34 Os NF-Y genes in rice, including 6 newly identified genes. Expression profile analysis covering the whole life cycle revealed that transcripts of Os NF-Y differentially accumulated in a tissue-specific,preferential or constitutive manner. In addition, gene duplication studies and expression analyses were performed to determine the evolutionary origins of the Os NF-Y gene *** Os NF-Y genes were differentially expressed after treatment of seedlings with one or more abiotic stresses such as drought, salt and cold. Analysis of expression correlation and Gene Ontology annotation suggested that Os NF-Y genes were co-expressed with genes that participated in stress, accumulation of seed storage reserves, and plant ***-expression analysis also revealed that Os NF-Y genes might interact with each other,suggesting that NF-Y subunits formed complexes that take part in transcriptional regulation. These results provide useful information for further elucidating the function of the NF-Y family and their regulatory pathways.

关键词： Abiotic stress Gene duplication Nuclear factor Y NF-Y Oryza sativa

Effects of altering the ratio of C16:0 and cis-9 C18:1 in rumen bypass fat on growth performance, lipid metabolism, intestinal barrier, cecal microbiota, and inflammation in fattening bulls

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Journal of Animal science and Biotechnology 2024年第5期15卷 2156-2174页

作者： Haixin Bai Haosheng Zhang Congwen Wang Modinat Tolani Lambo Yang Li Yonggen Zhang College of Animal science and technology Northeast Agricultural UniversityHarbin 150030China Faculty of Animal science and technology Yunnan Agricultural UniversityKunming 650500China

Background C16:0 and cis-9 C18:1 may have different effects on animal growth and health due to unique metabolism in *** study was investigated to explore the different effects of altering the ratio of C16:0 and cis-9 C18:1 in fat supplements on growth performance,lipid metabolism,intestinal barrier,cecal microbiota,and inflammation in fattening *** finishing Angus bulls(626±69 kg,21±0.5 months)were divided into 3 treatments according to the randomized block design:(1)control diet without additional fat(CON),(2)CON+2.5%palmitic acid calcium salt(PA,90%C16:0),and(3)CON+2.5%mixed fatty acid calcium salt(MA,60%C16:0+30%cis-9 C18:1).The experiment lasted for 104 d,after which all the bulls were slaughtered and sampled for *** MA tended to reduce 0–52 d dry matter intake compared to PA(DMI,P=0.052).Compared with CON and MA,PA significantly increased 0–52 d average daily gain(ADG,P=0.027).PA tended to improve the 0–52 d feed conversion rate compared with CON(FCR,P=0.088).Both PA and MA had no significant effect on 52–104 days of DMI,ADG and FCR(P>0.05).PA tended to improve plasma triglycerides compared with MA(P=0.077),significantly increased plasma cholesterol(P=0.002)and tended to improve subcutaneous adipose weight(P=0.066)when compared with CON and *** PA and MA increased visceral adipose weight compared with CON(P=0.021).Only PA increased the colonization of Rikenellaceae,Ruminococcus and Proteobacteria in the cecum,and MA increased Akkermansia abundance(Ppression of Claudin-1 in the jejunum(Ppression of TLR-4 and NF-κB

关键词： C16:0 cis-9 C18:1 Finishing bulls Intestinal homeostasis Lipid metabolism Low-grade inflammation