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Journal of Oceanology and Limnology 2024年第1期42卷 174-186页

作者： Yufei ZHANG Xianjun LI Yuhui LI Shiqi LIU Yanrui CHEN Miao JIA Xin WANG Lu ZHANG Qiping GAO Liang ZHANG Daoyong YU Baosheng GE State Key Laboratory of Heavy Oil Processing and Center for Bioengineering and biotechnology China University of Petroleum(East China)Qingdao 266580China Tongwei co. Ltd.Healthy Aquaculture Key Laboratory of Sichuan ProvinceChengdu 610093China

Light plays an important role in the photosynthesis and metabolic process of ***,how different light co.ditions regulate the biomass production and protein accumulation of microalgae is mostly *** this study,the influence of different light co.ditions,including light co.ors,densities,and light:dark cycles on the cell growth and biochemical co.position of Spirulina platensis was symmetrically *** different co.ored lights,*** all shows an increase trend within the increased light intensity ranges;however,each showing different optimal light *** the same light intensity,different co.ored lights show different growth rate of *** following the sequence of red>white>green>yellow>*** maximum growth rate and protein accumulation were determined as 21.88 and 5.10 mg/(L·d)when illuminated under red *** energy efficiency of different light sources was calculated and ranked as red>white>blue≈green>*** analysis suggests that red light can promote cell growth and protein accumulation by upregulating genes related to photosynthesis,carbon fixation,and C-N metabolism *** study provides a co.ducive and efficient way to promote biomass production and protein accumulation of *** by regulating light co.ditions.

关键词： microalgae light emitting diode protein accumulation biomass production transcriptomic analysis

Artificial Bear Bile:A Novel Approach to Balancing Medical Requirements and Animal Welfare

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Engineering 2024年第7期38卷 100-112页

作者： Yong Li Yuhong Huang Nan Feng Heping Zhang Jing Qu Shuanggang Ma Yunbao Liu Jiang Li Shaofeng Xu Ling Wang Mi Zhang Jie Cai Weiping Wang Ru Feng Hang Yu Bo Yu Dailiang Liang Heping Qin Suxiang Luo Yanfen Li Meifeng Li Ruihua Wang Chen Ma Yan Wang Xiaobo Cen Xiaoxian Xu Boli Zhang Xiaoliang Wang Shishan Yu State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia MedicaChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100050China The Seco.d Affiliated Hospital of Tianjin University of Traditional Chinese Medicine Tianjin 300250China Zhongshan Belling biotechnology co. Ltd.Zhongshan 528437China Zhongshan Anshi Biopharmaceutical co. Ltd.Zhongshan 528437China e WestChina-Frontier Pharma Tech Co.Ltd.Chengdu 610041China Tianjin University of Traditional Chinese Medicine Tianjin 301617China

Bear bile has been a valuable and effective medicinal material in traditional Chinese medicine(TCM)for over 13 ***,the current practice of obtaining it through bear farming is under scrutiny for its adverse impact on bear ***,we present a new approach for creating artificial bear bile(ABB)as a high-quality and sustainable alternative to natural bear *** study addresses the scientific challenges of creating bear bile alternatives through interdisciplinary co.laborations across various fields,including resources,chemistry,biology,medicine,pharmaco.ogy,and TCM.A co.prehensive efficacy assessment system that bridges the gap between TCM and modern medical terminology has been established,allowing for the systematic screening of therapeutic *** the utilization of chemical synthesis and enzyme engineering technologies,our research has achieved the environmentally friendly,large-scale production of bear bile therapeutic co.pounds,as well as the optimization and reco.position of ABB *** resulting ABB not only closely resembles natural bear bile in its co.position but also offers advantages such as co.sistent product quality,availability of raw materials,and independence from threatened or wild *** preclinical efficacy evaluations have demonstrated the equivalence of the therapeutic effects from ABB and those from co.mercially available drained bear bile(DBB).Furthermore,preclinical toxico.ogical assessment and phase I clinical trials show that the safety of ABB is on par with that of the currently used *** innovative strategy can serve as a new research paradigm for developing alternatives for other endangered TCMs,thereby strengthening the integrity and sustainability of TCM.

关键词： Artificial bear bile Chemical profile Formula optimization Pharmaco.ynamic co.sistency Preclinical toxico.ogical assessment

Unveiling the emerging role of curcumin to alleviate ochratoxin A-induced muscle toxicity in grass carp(Ctenopharyngodon idella):in vitro and in vivo studies

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Journal of Animal Science and biotechnology 2024年第4期15卷 1638-1656页

作者： Piao Zhao Lin Feng Weidan Jiang Pei Wu Yang Liu Hongmei Ren Xiaowan Jin Lu Zhang Haifeng Mi Xiaoqiu Zhou Animal Nutrition Institute Sichuan Agricultural UniversityChengdu 611130SichuanChina Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province Sichuan Agricultural UniversityChengdu 611130SichuanChina Key Laboratory of Animal Disease‑Resistance Nutrition Ministry of EducationMinistry of Agriculture and Rural AffairsKey Laboratory of Sichuan ProvinceChengdu 611130SichuanChina Tongwei co. Ltd.Healthy Aquaculture Key Laboratory of Sichuan ProvinceChengdu 610041SichuanChina

Background Ochratoxin A(OTA),a globally abundant and extremely hazardous pollutant,is a significant source of co.tamination in aquafeeds and is responsible for severe food *** developmental toxicity of OTA and the potential relieving strategy of natural products remain *** study screened the substance curcumin(Cur),which had the best effect in alleviating OTA inhibition of myoblast proliferation,from 96 natural products and investigated its effect and mechanism in reducing OTA myotoxicity in vivo and in *** A total of 720 healthy juvenile grass carp,with an initial average body weight of 11.06±0.05 g,were randomly assigned into 4 groups:the co.trol group(without OTA and Cur),1.2 mg/kg OTA group,400 mg/kg Cur group,and 1.2 mg/kg OTA+400 mg/kg Cur *** treatment co.sisted of 3 replicates(180 fish)for 60 *** Firstly,we cultured,purified,and identified myoblasts using the tissue block culture *** preliminary screening and re-screening of 96 substances,we examined cell proliferation-related indicators such as cell viability and ultimately found that Cur had the best ***,Cur co.ld alleviate OTA-inhibited myoblast differentiation and myofibrillar development-related proteins(Myo G and MYHC)in vivo and in vitro and improve the growth performance of grass ***,Cur co.ld also promote the expression of OTA-inhibited protein synthesis-related proteins(S6K1 and TOR),which was related to the activation of the AKT/TOR signaling ***,Cur co.ld downregulate the expression of OTA-enhanced protein degradation-related genes(murf1,foxo3a,and ub),which was related to the inhibition of the Fox O3a signaling *** In summary,our data demonstrated the effectiveness of Cur in alleviating OTA myotoxicity in vivo and in *** study co.firms the rapidity,feasibility,and effectiveness of establishing a natural product screening method targeting myoblasts to alleviate fungal toxin toxicity.

关键词： Curcumin Grass carp Myotoxic Ochratoxin A Protein deposition

Pseudomonas aeruginosa regulator PvrA binds simultaneously to multiple pseudo-palindromic sites for efficient transcription activation

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Science China(Life Sciences) 2024年第5期67卷 900-912页

作者： Yibo Zhu Bingnan Luo Xingyu Mou Yingjie Song Yonghong Zhou Yongbo Luo Bo Sun Youfu Luo Hong Tang Zhaoming Su Rui Bao Center of Infectious Diseases Division of Infectious Diseases in State Key Laboratory of BiotherapyWest China HospitalSichuan UniversityChengdu 610041China Department of Geriatrics and National Clinical Research Center for Geriatrics West China HospitalSichuan UniversityChengdu 610041China Key Laboratory of Biodiversity and Environment on the Qinghai-Tibetan Plateau Ministry of EducationSchool of Ecology and EnvironmentTibet UniversityLhasa 850000China Shanghai Advanced Research Institute Chinese Academy of SciencesShanghai 201210China co.lege of Life Science Sichuan Normal UniversityChengdu 610101China Accurate biotechnology(Hunan)co. LtdChangsha 410006China

Tetracycline repressor(TetR)family regulators(TFRs)are the largest group of DNA-binding transcription factors and are widely distributed in bacteria and *** play vital roles in co.trolling the expression of various genes and regulating diverse physiological ***,a TFR protein Pseudomonas virulence regulator A(PvrA),was identified from Pseudomonas aeruginosa as the transcriptional activator of genes involved in fatty acid utilization and bacterial ***,we show that PvrA can simultaneously bind to multiple pseudo-palindromic sites and upregulate the expression levels of target ***-electron microsco.y(cryo-EM)analysis indicates the simultaneous DNA reco.nition mechanism of PvrA and suggests that the bound DNA fragments co.sist of a distorted B-DNA double *** crystal structure and functional analysis of PvrA reveal a hinge region that secures the co.rect domain motion for reco.nition of the promiscuous ***,our results showed that mutations disrupting the regulatory hinge region have differential effects on biofilm formation and pyocyanin biosynthesis,resulting in attenuated bacterial ***,these findings will improve the understanding of the relationship between the structure and function of the TetR family and provide new insights into the mechanism of regulation of *** virulence.

关键词： Pseudomonas aeruginosa transcription regulator TetR protein-structure virulence

Pangenome and multi-tissue gene atlas provide new insights into the domestication and highland adaptation of yaks

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Journal of Animal Science and biotechnology 2024年第05期 1832-1850页

作者： Daoliang Lan Wei Fu Wenhui Ji Tserang-Donko Mipam Xianrong Xiong Shi Ying Yan Xiong Peng Sheng Jiangping Ni Lijun Bai Tongling Shan Xiangdong Kong Jian Li Ministry of Education of Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource and Utilization Southwest Minzu University co.lege of Animal & Veterinary Sciences Southwest Minzu University Institute of Qinghai-Tibetan Plateau Southwest Minzu University Jiguang Gene biotechnology co. Ltd. chengdu Genepre Technology co. Ltd. Shanghai Veterinary Research Institute Chinese Academy of Agricultural Sciences

Background The genetic diversity of yak, a key domestic animal on the Qinghai-Tibetan Plateau(QTP), is a vital resource for domestication and breeding efforts. This study presents the first yak pangenome obtained through the de novo assembly of 16 yak *** We disco.ered 290 Mb of nonreference sequences and 504 new genes. Our pangenome-wide presence and absence variation(PAV) analysis revealed 5,120 PAV-related genes, highlighting a wide range of variety-specific genes and genes with varying frequencies across yak populations. Principal co.ponent analysis(PCA) based on binary gene PAV data classified yaks into three new groups: wild, domestic, and Jinchuan. Moreover, we pro-posed a ‘two-haplotype genomic hybridization model' for understanding the hybridization patterns among breeds by integrating gene frequency, heterozygosity, and gene PAV data. A gene PAV-GWAS identified a novel gene(Bos-Gru3G009179) that may be associated with the multirib trait in Jinchuan yaks. Furthermore, an integrated transcrip-tome and pangenome analysis highlighted the significant differences in the expression of co.e genes and the muta-tional burden of differentially expressed genes between yaks from high and low altitudes. Transcriptome analysis across multiple species revealed that yaks have the most unique differentially expressed m RNAs and lnc RNAs(between high- and low-altitude regions), especially in the heart and lungs, when co.paring high- and low-altitude *** The yak pangenome offers a co.prehensive resource and new insights for functional genomic studies, supporting future biological research and breeding strategies.

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Impact of Low-temperature Storage on Volatile Flavor co.pounds in Prepared Pork Products

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Agricultural biotechnology 2024年第4期13卷 70-75,81页

作者： Xiulian WANG Jiamin ZHANG Ting BAI Wei WANG Kaihong YANG Lili JI co.lege of Food and Bioengineering Chengdu UniversityChengdu 610106China Meat Processing Key Laboratory of Sichuan Province Chengdu UniversityChengdu 610106China chengdu Hope Food co. Ltd.Chengdu 610000China

[Objectives]This study was co.ducted to explore the dynamic changes of volatile flavor co.pounds in prepared pork during storage at different low-temperature co.ditions.[Methods]Prepared pork was stored at 4,-4 and-18℃.The volatile flavor co.pounds of prepared pork were determined by solid-phase microextraction-gas chromatography-mass spectrometry(SPME-GC-MS)at days 0,7,14,21 and 28,and relative odor activity value(OAV),principal co.ponent analysis(PCA)and cluster analysis(CA)were co.bined to analyze changes in volatile flavor co.pounds of prepared pork during storage.[Results]The total number of volatile flavor co.pounds gradually decreased with the prolongation of the storage period,and OAV analysis identified 22 key flavor co.pounds(OAV≥1).The results of PCA and CA showed that 2-methyl-1-butanol,1-octen-3-ol,linalool,cineole,hexanal and nonanal were the main key flavor co.ponents,and the degree of flavor degradation was low under both superchilling and freezing *** 28 days of storage,the alco.ol co.tent in the chilling group was significantly higher than other two groups,and the overall co.tent of volatile flavor co.pounds was also significantly higher than other two groups,indicating that the-4℃chilling storage was more favorable for maintaining the overall flavor of prepared pork.[co.clusions]This study provides a theoretical basis for finding a better storage method for prepared meat products.

关键词： Low-temperature storage Prepared pork Volatile flavor co.ponent Principal co.ponent analysis Cluster analysis

Insights into genetic diversity and phenotypic variations in domestic geese through co.prehensive population and pan-genome analysis

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Journal of Animal Science and biotechnology 2024年第1期15卷 88-107页

作者： Guangliang Gao Hongmei Zhang Jiangping Ni Xianzhi Zhao Keshan Zhang Jian Wang Xiangdong Kong Qigui Wang Chongqing Academy of Animal Science Rongchang DistrictChongqing 402460China Livestock and Poultry Multi‑Omics Key Laboratory of Ministry of Agriculture and Rural Affairs College of Animal Science and TechnologySichuan Agricultural UniversityChengdu 611130China Chongqing Engineering Research Center of Goose Genetic Improvement Rongchang DistrictChongqing 402460China Department of Cardiovascular Ultrasound and Non‑Invasive Cardiology Sichuan Academy of Medical Sciences and Sichuan Provincial People’s HospitalUniversity of Electronic Science and Technology of ChinaChengdu611731China Ultrasound in Cardiac Electrophysiology and Biomechanics Key Laboratory of Sichuan Province University of Electronic Science and Technology of ChinaChengdu611731China JiguangGene biotechnology co. Ltd.Nanjing210032China Jiangsu Agri-Animal Vocational co.lege Taizhou225300China Poultry Science Institute Chongqing Academy of Animal ScienceNo.51 Changzhou AvenueRongchang DistrictChongqing 402460P.R.China

Background Domestic goose breeds are descended from either the Swan goose(Anser cygnoides)or the Greylag goose(Anser anser),exhibiting variations in body size,reproductive performance,egg production,feather co.or,and other phenotypic *** a pan-genome facilitates a thorough identification of genetic variations,thereby deepening our co.prehension of the molecular mechanisms underlying genetic diversity and phenotypic *** To co.prehensively facilitate population genomic and pan-genomic analyses in geese,we embarked on the task of 659 geese whole genome resequencing data and co.piling a database of 155 RNA-seq *** co.structing the pan-genome for geese,we generated non-reference co.tigs totaling 612 Mb,unveiling a co.lection of 2,813 novel genes and pinpointing 15,567 co.e genes,1,324 softco.e genes,2,734 shell genes,and 878 cloud genes in goose ***,we detected an 81.97 Mb genomic region showing signs of genome selection,enco.passing the TGFBR2 gene co.related with variations in body weight among ***-wide association studies utilizing single nucleotide polymorphisms(SNPs)and presence-absence variation revealed significant genomic associations with various goose meat quality,reproductive,and body co.position *** instance,a gene enco.ing the SVEP1 protein was linked to carcass oblique length,and a distinct gene-CDS haplotype of the SVEP1 gene exhibited an association with carcass oblique ***,the pan-genome analysis revealed enrichment of variable genes in the“hair follicle maturation”Gene Ontology term,potentially linked to the selection of feather-related traits in geese.A gene presence-absence variation analysis suggested a reduced frequency of genes associated with“regulation of heart co.traction”in domesticated geese co.pared to their wild *** study provided novel insights into gene expression features and functions by integrating gene expression patterns across multiple orga

关键词： Gene-CDS haplotype Goose GWAS Pan-genome Presence-absence variation Selection signal

Phase I clinical trial of intracerebral injection of lentiviral-ABCD1 for the treatment of cerebral adrenoleukodystrophy

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Science Bulletin 2024年第16期69卷 2596-2603页

作者： Qiu-Hong Wang Jing Wanga Zhi-Pei Ling Zhi-Qiang Cui Jie Gong Rui Zhang Shi-Jun Li Yang-Yang Wang Rui Yang De-Hui Huang Wen He Jing Gao Chen Feng Pei-Li Hu Li-Ying Liu Lung-Ji Chang Li-Ping Zou Senior Department of Pediatrics Chinese PLA General HospitalBeijing 100853China Medical School of Chinese PLA Beijing 100853China Department of Neurosurgery Hainan Hospital of PLA General HospitalSanya 572013China Department of Neurosurgery The First Medical Center of Chinese PLA General HospitalBeijing 100853China chengdu Women’s and Children’s Central Hospital School of MedicineUniversity of Electronic Science and Technology of ChinaChengdu 611731China School of Medicine University of Electronic Science and Technology of ChinaChengdu 610054China Beijing Meikang biotechnology co. LTD.Beijing 100085China Department of Radiology The First Medical Center of Chinese PLA General HospitalBeijing 100853China Department of Neurology The First Medical Center of Chinese PLA General HospitalBeijing 100853China Department of Pediatrics Xuanwu HospitalCapital Medical UniversityBeijing 100053China Shenzhen Geno-Immune Medical Institute Shenzhen 518057China Beijing Institute for Brain Disorders Center for Brain Disorders Research Capital Medical UniversityBeijing 100069China

This was a single-arm,multicenter,open-label phase I *** vectors(LV)carrying the ABCD1 gene(LV-ABCD1)was directly injected into the brain of patients with childhood cerebral adrenoleukodystrophy(CCALD),and multi-site injection was *** injection dose increased from 200 to 1600 lL(vector titer:1×10^(9) transduction units per mL(TU/mL)),and the average dose per kilogram body weight ranges from 8 to 63.6 lL/*** primary endpoint was safety,dose-exploration and immunogenicity and the seco.dary endpoint was initial evaluation of efficacy and the expression of ABCD1 protein.A total of 7 patients participated in this phase I study and were followed for 1 *** injectionrelated serious adverse event or death *** adverse events associated with the injection were irritability(71%,5/7)and fever(37.2-38.5℃,57%,4/7).Adverse events were mild and selflimited,or resolved within 3 d of symptomatic *** maximal tolerable dose is 1600 *** 5 cases(83.3%,5/6),no lentivirus associated antibodies were *** overall survival at 1-year was 100%.The ABCD1 protein expression was detected in neutrophils,monocytes and *** study suggests that the intracerebral injection of LV-ABCD1 for CCALD is safe and can achieve successful LV transduction in vivo;even the maximal dose did not increase the risk of adverse ***,the direct LV-ABCD1 injection displayed low *** addition,the effectiveness of intracerebral LV-ABCD1 injection has been preliminarily demonstrated while further investigation is *** study has been registered in the Chinese Clinical Trial Registry(https://***/,registration number:ChiCTR1900026649).

关键词： Adrenoleukodystrophy Lentiviral vectors ABCD1 Intracerebral injection

Insights into the mechanism of L-malic acid on drip loss of chicken meat under co.mercial co.ditions

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Journal of Animal Science and biotechnology 2024年第3期15卷 1312-1324页

作者： Haijun Sun Xue Yan Lu Wang Ruimin Zhu Meixia Chen Jingdong Yin Xin Zhang State Key Laboratory of Animal Nutrition and Feeding College of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China New Hope Liuhe co. Ltd./Key Laboratory of Feed and Livestock and Poultry Products Quality&Safety ControlMinistry of AgricultureChengduSichuan 610023China Institute of Animal Husbandry and Veterinary Medicine Beijing Academy of Agriculture and Forestry SciencesBeijing 100097China

Background A deterioration in the meat quality of broilers has attracted much more attention in recent years.L-malic acid(MA)is evidenced to decrease meat drip loss in broilers,but the underlying molecular mechanisms are still ***’s also not sure whether the outputs obtained under experimental co.ditions can be obtained in a co.-mercial ***,we investigated the effects and mechanisms of dietary MA supplementation on chicken meat drip loss at large-scale *** Results showed that the growth performance and drip loss were improved by MA *** metabolome revealed that L-2-aminoadipic acid,β-aminoisobutyric acid,eico.apentaenoic acid,and nico.inamide,as well as amino acid metabolism pathways co.nected to the improvements of meat quality by MA *** transcriptome analysis further indicated that the effect of MA on drip loss was also related to the proper immune response,evidenced by the enhanced B cell receptor signaling pathway,NF-κB signaling pathway,TNF signaling pathway,and IL-17 signaling *** We provided evidence that MA decreased chicken meat drip loss under co.mercial *** and transcriptome revealed a co.prehensive understanding of the underlying ***,MA co.ld be used as a promising dietary supplement for enhancing the water-holding capacity of chicken meat.

关键词： Drip loss Immune response L-malic acid Meat quality Metabolome Transcriptome

Bacterial and cancerous cell membrane fused liposome co.rdinates with PD-L1 inhibitor for cancer immunotherapy

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Nano Research 2024年第9期17卷 8389-8401页

作者： Xianjin Luo Chenglong Li Zhaofei Guo Hairui Wang Penghui He Yuanhao Zhao Yi Lin Chunting He Yingying Hou Yongshun Zhang Guangsheng Du Key Laboratory of Drug-Targeting and Drug Delivery System of the Education Ministry and Sichuan Province Sichuan Engineering Laboratory for Plant-Sourced Drug and Sichuan Research Center for Drug Precision Industrial TechnologyWest China School of PharmacySichuan UniversityChengdu 610064China Pharmaceutical biotechnology Department of PharmacyLMU MunichButenandt Strasse 5-13Munich 81377Germany Department of Biomedical Engineering College of Future TechnologyPeking UniversityBeijing 100871China

Although tumor cell membranes with broad-spectrum antigens have been explored for cancer vaccines for decades,their relatively poor capacity to stimulate immune responses,especially cellular immune responses,has limited their ***,we presented a novel bacterial and cancerous cell membrane fusogenic liposome for co.delivering cell membrane-derived antigens and ***,a programmed death-ligand 1(PD-L1)inhibitor,JQ-1,was inco.porated into the formulation to tackle the up-regulated PD-L1 expression of antigen-presenting cells(APCs)upon vaccination,thereby augmenting its anti-tumor *** fusogenic liposomes demonstrated significantly improved cellular uptake by APCs and effectively suppressed PD-L1 expression in bone marrow-derived dendritic cells(BMDCs)in *** subcutaneous vaccination,the nano-vaccines efficiently drained to the tumor-draining lymph nodes(TDLNs),and significantly inhibited PD-L1 expression of both dendritic cells(DCs)and macrophages within the TDLNs and *** a result,the liposomal vaccine induced robust innate and cellular immune responses and inhibited tumor growth in a co.orectal carcinoma-burden mouse *** summary,the fabricated cell membrane-based fusogenic liposomes offer a safe,effective,and easily applicable strategy for tumor immunotherapy and hold potential for personalized cancer immunotherapy.

关键词： fusogenic liposome tumor cell membrane adjuvant programmed death-ligand 1(PD-L1)inhibitor tumor draining lymph nodes