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Revealing the formation mechanism of umami peptides in fermented broad bean paste based on peptidomics and macrogenomics

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food Science and Human Wellness 2024年

作者： Yuqing Xie Jianhua Zhao Binbin Zhou Ping Liu Jie Zhao Min Xu Jiaxin Chen Hongbin Lin Jie Tang School of food and Bio-engineering， Xihua University Chongqing Key Laboratory of Specialty food co.Built by Sichuan and Chongqing Chengdu food Microbiology Key Laboratory of Sichuan Province

Umami peptides play important roles in the flavor of fermented broad bean paste (FBBP)， and proteases produced by microorganisms co.tributed to the production of umami peptides. In order to reveal the formation of umami peptides and their relationships with protease-producing microorganisms during the natural fermentation of FBBP， peptidomics and virtual screening were used to identify and screen umami peptides. Meanwhile， macrogenomics was used to analyze the abundance of microbial-derived protease genes during FBBP fermentation. Then， based on the Pearson co.relation co.fficient， the co.relation network diagram of each protease-producing microorganism with umami peptides was co.structed. The results showed that a total of two exopeptidases and four endopeptidases were annotated from FBBP. Staphyloco.cus， Lactobacillus， Aspergillus， and Weissella can produce most proteases. The species Lactobacillus＿curvatus， Dyella＿jiangningensis， Erythrobacter＿sp， and unclassified＿g＿Pantoea had strong co.relation with umami peptides， and they may co.tribute to the process of protein hydrolysis to produce umami peptides. This study is expected to reveal the formation mechanism of umami peptides in FBBP， and the results of this study provided a better understanding of the relationship between proteases， microbiota， and co.e umami peptides in FBBP， which co.ld help to improve the umami taste of Pixian Douban paste (PDBP) during fermentation.

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Insights into the mechanism of L-malic acid on drip loss of chicken meat under co.mercial co.ditions

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Journal of Animal Science and Biotechnology 2024年第3期15卷 1312-1324页

作者： Haijun Sun Xue Yan Lu Wang Ruimin Zhu Meixia Chen Jingdong Yin Xin Zhang State Key Laboratory of Animal Nutrition and Feeding College of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China New hope Liuhe co. Ltd./Key Laboratory of Feed and Livestock and Poultry Products Quality&Safety ControlMinistry of AgricultureChengduSichuan 610023China Institute of Animal Husbandry and Veterinary Medicine Beijing Academy of Agriculture and Forestry SciencesBeijing 100097China

Background A deterioration in the meat quality of broilers has attracted much more attention in recent years.L-malic acid(MA)is evidenced to decrease meat drip loss in broilers,but the underlying molecular mechanisms are still ***’s also not sure whether the outputs obtained under experimental co.ditions can be obtained in a co.-mercial ***,we investigated the effects and mechanisms of dietary MA supplementation on chicken meat drip loss at large-scale *** Results showed that the growth performance and drip loss were improved by MA *** metabolome revealed that L-2-aminoadipic acid,β-aminoisobutyric acid,eico.apentaenoic acid,and nico.inamide,as well as amino acid metabolism pathways co.nected to the improvements of meat quality by MA *** transcriptome analysis further indicated that the effect of MA on drip loss was also related to the proper immune response,evidenced by the enhanced B cell receptor signaling pathway,NF-κB signaling pathway,TNF signaling pathway,and IL-17 signaling *** We provided evidence that MA decreased chicken meat drip loss under co.mercial *** and transcriptome revealed a co.prehensive understanding of the underlying ***,MA co.ld be used as a promising dietary supplement for enhancing the water-holding capacity of chicken meat.

关键词： Drip loss Immune response L-malic acid Meat quality Metabolome Transcriptome

Temperature-Induced Unfolding Pathway of Staphyloco.cal Enterotoxin B:Insights from Circular Dichroism and Molecular Dynamics Simulation

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食品科学 2024年第18期45卷 55-76页

作者： LIU Ji ZHANG Shiyu ZENG Yu DENG Yi Institute of Qinghai-Tibetan Plateau Southwest Minzu UniversityChengdu 610041China co.lege of food Sciences and Technology Southwest Minzu UniversityChengdu 610041China Wuliangye Group co.Ltd. Yibin 644000China

In this study,circular dichroism(CD)and molecular dynamics(MD)simulation were used to investigate the thermal unfolding pathway of staphyloco.cal enterotoxin B(SEB)at temperatures of 298–371 and 298–500 K,and the relationship between the experimental and simulation results were *** co.putational findings on the seco.dary structure of SEB showed that at room temperature,the CD spectrosco.ic results were highly co.sistent with the MD ***,under heating co.ditions,the changing trends of helix,sheet and random co.l obtained by CD spectral fitting were highly co.sistent with those obtained by *** order to gain a deeper understanding of the thermal stability mechanism of SEB,the MD trajectories were analyzed in terms of root mean square deviation(RMSD),seco.dary structure assignment(SSA),radius of gyration(R_(g)),free energy surfaces(FES),solvent-accessible surface area(SASA),hydrogen bonds and salt *** results showed that at low heating temperature,domain Ⅰ without loops(omitting the mobile loop region)mainly relied on hydrophobic interaction to maintain its thermal stability,whereas the thermal stability of domain Ⅱ was mainly co.trolled by salt bridges and hydrogen *** high heating temperature co.ditions,the hydrophobic interactions in domain Ⅰ without loops were destroyed and the seco.dary structure was almost co.pletely lost,while domain Ⅱ co.ld still rely on salt bridges as molecular staples to barely maintain the stability of the seco.dary *** results help us to understand the thermodynamic and kinetic mechanisms that maintain the thermal stability of SEB at the molecular level,and provide a direction for establishing safer and more effective food sterilization processes.

关键词： staphyloco.cal enterotoxin B circular dichroism molecular dynamics simulations temperature-induced unfolding

Response and assembly of abundant and rare taxa in Zaopei under different co.bination patterns of Daqu and pit mud:from microbial eco.ogy to Baijiu brewing microeco.ystem

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food Science and Human Wellness 2024年第3期13卷 1439-1452页

作者： Yu Mu Jun Huang Rongqing Zhou Suyi Zhang Hui Qin Hanlan Tang Qianglin Pan Huifang Tang co.lege of Biomass Science and Engineering Sichuan UniversityChengdu 610065China National Engineering Research Center of Solid-State Manufacturing Luzhou 646000China Luzhou Laojiao co. Ltd.Luzhou 646699China

The quality and aroma of strong-flavor Baijiu are mainly dependent on Daqu,pit mud(PM),and the interaction of ***,little is known about how their co.bination patterns affect the microbiome and metabolome of Zaopei,especially the metabolic function of rare ***,an experiment on industrial size was designed to assess the effects of 6 co.binations(3 kinds of Daqu×2 kinds of PM)on the co.position and assembly of different taxa,as well as the flavor *** results showed that Zaopei's microbiota was co.posed of a few abundant taxa and enormous rare taxa,and rare bacterial and abundant fungal subco.munities were significantly affected by co.bination *** assembly processes of abundant/rare taxa and bacterial/fungal co.munities were distinct,and environmental changes mediated the balance between stochastic and deterministic processes in rare bacteria ***,specific co.bination patterns improved the flavor quality of Zaopei by enhancing the interspecies interaction,which was closely related to rare taxa,especially rare *** findings highlighted that rare bacteria might be the keystone in involving co.munity interaction and maintaining metabolic function,which provided a scientific foundation for better understanding and regulating the brewing microbiota from the viewpoint of microbial eco.ogy.

关键词： Bioaugmented Daqu Artificial pit mud Abundant and rare taxa co.munity assembly Metabolic function

Unveiling the emerging role of curcumin to alleviate ochratoxin A-induced muscle toxicity in grass carp(Ctenopharyngodon idella):in vitro and in vivo studies

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Journal of Animal Science and Biotechnology 2024年第4期15卷 1638-1656页

作者： Piao Zhao Lin Feng Weidan Jiang Pei Wu Yang Liu Hongmei Ren Xiaowan Jin Lu Zhang Haifeng Mi Xiaoqiu Zhou Animal Nutrition Institute Sichuan Agricultural UniversityChengdu 611130SichuanChina Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province Sichuan Agricultural UniversityChengdu 611130SichuanChina Key Laboratory of Animal Disease‑Resistance Nutrition Ministry of EducationMinistry of Agriculture and Rural AffairsKey Laboratory of Sichuan ProvinceChengdu 611130SichuanChina Tongwei co. Ltd.Healthy Aquaculture Key Laboratory of Sichuan ProvinceChengdu 610041SichuanChina

Background Ochratoxin A(OTA),a globally abundant and extremely hazardous pollutant,is a significant source of co.tamination in aquafeeds and is responsible for severe food *** developmental toxicity of OTA and the potential relieving strategy of natural products remain *** study screened the substance curcumin(Cur),which had the best effect in alleviating OTA inhibition of myoblast proliferation,from 96 natural products and investigated its effect and mechanism in reducing OTA myotoxicity in vivo and in *** A total of 720 healthy juvenile grass carp,with an initial average body weight of 11.06±0.05 g,were randomly assigned into 4 groups:the co.trol group(without OTA and Cur),1.2 mg/kg OTA group,400 mg/kg Cur group,and 1.2 mg/kg OTA+400 mg/kg Cur *** treatment co.sisted of 3 replicates(180 fish)for 60 *** Firstly,we cultured,purified,and identified myoblasts using the tissue block culture *** preliminary screening and re-screening of 96 substances,we examined cell proliferation-related indicators such as cell viability and ultimately found that Cur had the best ***,Cur co.ld alleviate OTA-inhibited myoblast differentiation and myofibrillar development-related proteins(Myo G and MYHC)in vivo and in vitro and improve the growth performance of grass ***,Cur co.ld also promote the expression of OTA-inhibited protein synthesis-related proteins(S6K1 and TOR),which was related to the activation of the AKT/TOR signaling ***,Cur co.ld downregulate the expression of OTA-enhanced protein degradation-related genes(murf1,foxo3a,and ub),which was related to the inhibition of the Fox O3a signaling *** In summary,our data demonstrated the effectiveness of Cur in alleviating OTA myotoxicity in vivo and in *** study co.firms the rapidity,feasibility,and effectiveness of establishing a natural product screening method targeting myoblasts to alleviate fungal toxin toxicity.

关键词： Curcumin Grass carp Myotoxic Ochratoxin A Protein deposition

Antimicrobial resistance characteristics and phylogenetic relationships of pleuromutilin-resistant Enteroco.cus isolates from different environmental samples along a laying hen production chain

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Journal of Environmental Sciences 2024年第3期137卷 195-205页

作者： co.g Lin Yuxuan Feng Xianjun Xie Haoyu Zhang Jie Wu Yixiao Zhu Jing Yu Jingyi Feng Wen Su Shanming Lai Anyun Zhang Animal Disease Prevention and food Safety Key Laboratory of Sichuan Province Key Laboratory of Bio-Resource and Eco-Environment of Ministry of EducationCollege of Life SciencesSichuan UniversityChengdu 610065China

Antimicrobial resistance in the laying hen production industry has beco.e a serious public health *** antimicrobial resistance and phylogenetic relationships of the co.mon co.ditional pathogen Enteroco.cus along the laying hen production chain have not been systematically clarified.105 Enteroco.cus isolates were obtained from 115 environmental samples (air,dust,feces,fies,sewage,and soil) co.lected along the laying hen production chain (breeding chicken,chick,young chicken,and co.mercial laying hen).These Enteroco.cus isolates exhibited resistance to some clinically relevant antibiotics,such as tetracycline (92.4%),streptomycin (92.4%),and erythromycin (91.4%),and all strains had multidrug resistance *** genome sequencing characterized 29 acquired antibiotic resistance genes (ARGs) that co.ferred resistance to 11 classes of antibiotics in 51pleuromutilin-resistant Enteroco.cus isolates,and lsa(E),which mediates resistance to pleuromutilins,always co.occurred with lnu(B).Alignments with the Mobile Genetic Elements database identified four transposons (Tn554,Tn558,Tn6261,and Tn6674) with several ARGs(erm(A),ant(9)-la,fex(A),and optrA) that mediated resistance to many clinically important ***,we identified two new transposons that carried ARGs in the Tn554 family designated as Tn7508 and Tn7492.A co.plementary approach based on co.ventional multi-locus sequence typing and whole genome single nucleotide polymorphism analysis showed that phylogenetically related pleuromutilin-resistant Enteroco.cus isolates were widely distributed in various environments on different production *** results indicate that environmental co.tamination by antimicrobial-resistant Enteroco.cus requires greater attention,and they highlight the risk of pleuromutilin-resistant Enteroco.cus and ARGs disseminating along the laying hen production chain,thereby warranting effective disinfection.

关键词： Antimicrobial resistance Enteroco.cus Laying hen production chain Pleuromutilin

Luteolin alleviates sorafenib-induced ferroptosis of BRL-3A cells through modulation of the Nrf2/GPX4 signaling pathway

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Traditional Medicine Research 2024年第10期9卷 1-9页

作者： Bo-Wen Zhang Di Yang Jin-Tao Li Mei-Hao Peng Jia-Qing Liao Qi Zhao Yi-Xi Yang Qiu-Xia Lu Engineering Research Center of Sichuan-Tibet Traditional Medicinal Plant Chengdu UniversityChengdu 610106China School of food and Biological Engineering Chengdu UniversityChengdu 610106China School of Pharmacy Chengdu UniversityChengdu 610106China

Background:Luteolin is a flavonoid chemical that exists in a variety of medicinal and edible plants and holds many biologically active properties in liver protection,anti-cancer,antioxidants,anti-inflammatory,neuroprotective,*** to its hepatoprotective properties,luteolin was selected to co.treat with sorafenib,one of the approved protein kinase inhibitors,to reduce sorafenib-induced normal liver cell ***:The BRL-3A cell line was treated with sorafenib to establish a liver injury model,followed by luteolin *** cell viability was detected,and the mechanism of action was detected by immunofluorescence,western blotting,and real-time quantitative ***:The research findings demonstrated that luteolin co.ld increase cystine/glutamate transporter xCT(SLC7A11)and glutathione peroxidase 4(GPX4)expression and display a chelating effect on iron,which led to increased glutathione and decreased malondialdehyde,Fe^(2+) and lipid reactive oxygen species co.tents in BRL-3A cells,and the sorafenib-induced mitochondrial membrane potential decrease was also *** addition,when sorafenib caused the accumulation of lipid reactive oxygen species,luteolin co.ld help release this oxidative stress by activating nuclear factor E2-related factor 2(Nrf2)and up-regulating the expression of the associated genes heme oxygenase 1(HO-1)and quinone oxidoreductase 1(NQO1).co.clusion:Therefore,luteolin may ameliorate sorafenib-induced ferroptosis by activating the Nrf2-associated pathway without any impact on sorafenib anti-cancer *** can be used as an adjuvant to sorafenib to reduce liver injury in patients with hepatocellular carcinoma.

关键词： luteolin sorafenib liver injury ferroptosis Nrf2/GPX4

The flavonoids from Pentorum chinense *** ferroptosis to alleviate sorafenib-induced liver injury in BRL-3A cells

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Traditional Medicine Research 2024年第2期9卷 18-26页

作者： Di Yang Bo-Wen Zhang Shan Lei Jin-Tao Li Mei-Hao Peng Jia-Qing Liao Xue Tang Qi Zhao Qiu-Xia Lu Engineering Research Center of Sichuan-Tibet Traditional Medicinal Plant Chengdu UniversityChengdu 610106China School of food and Biological Engineering Chengdu UniversityChengdu 610106China School of Pharmacy Chengdu UniversityChengdu 610106China chengdu Analytical Applications Center Shimadzu(China)Co.Ltd.Chengdu 610023China

Background:Drug-induced liver damage is a severe medical issue that affects people all over the *** has some side effects that cause liver injury.A dietary medicinal plant called Penthorum chinense Pursh.(PCP)has hepatoprotective *** are currently few reports on PCP’s protective impact and mechanism against sorafenib-induced liver ***:To create a liver injury model,sorafenib was administered to BRL-3A *** viability assays,immunofluorescence tests,Western blotting,real-time quantitative PCR,and high-co.tent imaging systems were utilized to examine PCP’s effect and ***:In this study,PCP treatment mitigated the liver damage caused by sorafenib by enhancing cell survival,lowering lipid reactive oxygen species and malondialdehyde levels,and elevating glutathione *** addition,PCP can enhance the protein expression of cystine/glutamate transporter xCT and glutathione peroxidase 4,reduce iron co.tent and alleviate mitochondrial *** mechanism studies revealed that PCP inhibited ferroptosis by promoting the production of nuclear factor E2-related factor 2 nuclear translocation and subsequently affecting target genes(HO-1 and NQO1).co.clusion:Together,PCP regulates the nuclear factor E2-related factor 2 pathway,which helps to lessen ferroptosis brought on by sorafenib.

关键词： sorafenib Pentorum chinense Pursh. ferroptosis oxidative stress Nrf2

Effects of Potassium-Solubilizing Bacteria on Growth, Antioxidant Activity and Expression of Related Genes in Fritillaria taipaiensis P. Y. Li

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Phyton-International Journal of Experimental Botany 2024年第4期93卷 789-806页

作者： Jiaqi Lang Mingyan Ye Ya Luo Yueheng Wang Zhifen Shi Xiaotian Kong Xuan Li Nong Zhou Chongqing Engineering Laboratory of Green Planting and Deep Processing of Famous-Region Drug in the Three Gorges Reservoir Region College of Biology and Food EngineeringChongqing Three Gorges UniversityChongqing404120China co.lege of Modern Chinese Medicine Industry Chengdu University of Traditional Chinese MedicineChengdu611130China co.lege of Pharmacy Dali UniversityDali671000China

This study aimed to examine the effects of inoculating Fritillaria taipaiensis *** leaves with different strains ofpotassium-solubilizing bacteria (KSB), or co.binations thereof, focusing on aspects of photosynthesis and physiologicaland biochemical characteristics. At present, some studies have only studied the rhizosphere microbialco.munity characteristics of F. taipaiensis and have not discussed the effects of different microbial species on thegrowth promotion of F. taipaiensis. This paper will start from the perspective of potassium-solubilizing bacteria toco.duct an in-depth study. Seed cultivation co.menced at the base with three different KSBs in early October2022. The growth of F. taipaiensis leaves was observed after different treatments. Both single-plant and co.poundinoculations were executed. A total of eight treatment groups were established, with aseptic fertilizer and sterilizedsoil functioning as the co.trol group. The results reveal that intercellular co.(2) co.centration (Ci), stomatal co.ductance(Gs), and transpiration rate (Tr) were at their apex in the S7 group. Most treatment groups exhibited anincrease in leaf area, photosynthetic pigment co.tent, soluble sugar, soluble protein, Superoxide Dismutase(SOD), Peroxidase (POD), Catalase (CAT) activities, and proline co.tent. The expression levels of POD, SOD,and CAT genes were evaluated, following inoculation with different KSB. The highest was the S7 group. Theinoculation with various KSB, or co.binations thereof, appears to bolster the growth and development of F. *** co.posite inoculation group S7, co.prising Bacillus cereus, Burkholderia cepacia, and Bacillus subtilis,manifested the most favorable impact on the diverse indices of F. taipaiensis, thereby furnishing valuableinsights for the selection of bacterial fertilizer in the artificial cultivation of F. taipaiensis.

关键词： Fritillaria taipaiensis bacteria antioxidant enzyme genes leaf physiology and biochemistry photosynthetic characteristics