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Recombinant Helicobacter pylori catalase

World Journal of Gastroenterology 2003年第5期9卷 1119-1122页

作者： YangBai Ya-LiZhang Jian-FengJin Ji-DeWang Zhao-ShanZhang Dian-YuanZhou InstituteforDigestiveMedicine NanfangHospitaltheFirstMilitaryMedicalUniversityGuangzhou510515GuangdongProvinceChina chemistryuniversityofbeijing Beijing100071China InstituteofBiotechnology ChineseAcademyofMilitaryMedicalSciencesBeijing100071China

AIM: To construct a recombinant strain which highly expresses catalase of Helicobacter pylori(***) and assay the activity of H. pylori ***: The catalase DNA was amplified from H. pylori chromosomal DNA with PCR techniques and inserted into the prokaryotie expression vector pET-22b (+), and then was transformed into the BL21 (DE3) E. coli strain which expressed catalase recombinant protein. The activity of *** catalase was assayed by the Beers & ***: DNA sequence analysis showed that the sequence of catalase DNA was the same as GenBank's research. The catalase recombinant protein amounted to 24.4 % of the total bacterial protein after induced with IPTG for 3 hours at 37 ℃ and the activity of H. pylori catalase was high in the BL21 (DE3) E. coli ***: A clone expressing high activity H. pylori catalase is obtained, laying a good foundation for further studies.

关键词：幽门螺杆菌过氧化氢酶基因重组 DNA扩增聚合酶链反应基因库 DNA序列分析

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Expression of Helicobacter pylori Hsp60 protein and its immunogenicity

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World Journal of Gastroenterology 2003年第12期9卷 2711-2714页

作者： YangBai Liang-RenLi Ji-DeWang YeChen Jian-FengJin Zhao-ShanZhang, Dian-YuanZhou Ya-LiZhang PLAInstituteforDigestiveMedicine NanfangHospitalFirstMilitaryMedicalUniversityGuangzhou510515GuangdongProvinceChina chemistryuniversityofbeijing Beijing100071China InstituteofBiotechnology AcademyofMilitaryMedicalScienceBeijing100071China

AIM: To express Hsp60 protein of H pylori by a constructed vector and to evaluate its ***: Hsp60 DNA was amplified by PCR and inserted into the prokaryotie expression vector pET-22b (+), which was transformed into BL21 (DE3) E. coli strain to express recombinant protein. Immunogenicity of expressed Hsp60 protein was evaluated with animal ***: DNA sequence analysis showed Hsp60 DNA was the same as GenBank's research. Hsp60 recombinant protein accounted for 27.2 % of the total bacterial protein,and could be recognized by the serum from H pylori infected patients and Balb/c mice immunized with Hsp60 ***: Hsp60 recombinant protein might become a potential vaccine for controlling and treating H pylori infection.

关键词：热休克蛋白60 幽门螺杆菌免疫原性 PCR DNA序列分析

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Cloning and expression and immunogenicity of Helicobacter pylori BabA_2 gene

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World Journal of Gastroenterology 2004年第17期10卷 2560-2562页

作者： YangBai Ya-LiZhang YeChen Jian-FengJin Zhao-ShanZhang Dian-YuanZhou PLAInstituteforDigestiveMedicine NanfangHospitaltheFirstMilitaryMedicalUniversityGuangzhou510515GuangdongProvinceChina chemistryuniversityofbeijing Beijing100071China InstituteofBiotechnology AcademyofMilitaryMedicalSciencesBeijing100071China

AIM: To construct a recombinant strain which expresses BabA of Helicobacter pylori(H pylori) and to study the immunogenicity of ***: BabA2 DNA was amplified by PCR and inserted into the prokaryotie expression vector pET-22b (+) and expressed in the BL21 (DE3) *** strain. Furthermore,BabA immunogenicity was studied by animal ***: DNA sequence analysis showed the sequence of BabA2 DNA was the same as the one published by *** BabA recombinant protein accounted for 34.8% of the total bacterial protein. The serum from H pylori infected patients and Balb/c miced immunized with BabA itself could recognize ***: BabA recombinant protein may be an potential vaccine for control and treatment of Hpyloriinfection.

关键词：无性繁殖基因表达免疫原性哈比特属幽门菌 BabA2基因重组细胞蛋白

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