检索结果-南通市图书馆

Endoplasmic reticulum stress and autophagy in cerebral ischemia/reperfusion injury:PERK as a potential target for intervention

引用

Neural Regeneration Research 2025年第5期20卷 1455-1466页

作者： Ju Zheng Yixin Li Ting Zhang Yanlin Fu Peiyan Long Xiao Gao Zhengwei Wang Zhizhong Guan Xiaolan Qi Wei Hong Yan Xiao Key Laboratory of Endemic and Ethnic diseases Ministry of Education&Key Laboratory of Medical Molecular Biology of Guizhou ProvinceGuizhou Medical UniversityGuiyangGuizhou ProvinceChina Guizhou center for Disease control and prevention GuiyangGuizhou ProvinceChina Department of Histology and Embryology Guizhou Medical UniversityGuiyangGuizhou ProvinceChina Collaborative Innovation center for prevention and control of Endemic and Ethnic Regional diseases Co-constructed by the Province and Ministry GuiyangGuizhou ProvinceChina

Several studies have shown that activation of unfolded protein response and endoplasmic reticulum(ER)stress plays a crucial role in severe cerebral ischemia/reperfusion *** occurs within hours after cerebral ischemia,but the relationship between ER stress and autophagy remains *** this study,we established experimental models using oxygen-glucose deprivation/reoxygenation in PC12 cells and primary neurons to simulate cerebral ischemia/reperfusion *** found that prolongation of oxygen-glucose deprivation activated the ER stress pathway protein kinase-like endoplasmic reticulum kinase(PERK)/eukaryotic translation initiation factor 2 subunit alpha(e IF2α)-activating transcription factor 4(ATF4)-C/EBP homologous protein(CHOP),increased neuronal apoptosis,and induced ***,inhibition of ER stress using inhibitors or by si RNA knockdown of the PERK gene significantly attenuated excessive autophagy and neuronal apoptosis,indicating an interaction between autophagy and ER stress and suggesting PERK as an essential target for regulating *** autophagy with chloroquine exacerbated ER stress-induced apoptosis,indicating that normal levels of autophagy play a protective role in neuronal injury following cerebral ischemia/reperfusion *** from this study indicate that cerebral ischemia/reperfusion injury can trigger neuronal ER stress and promote autophagy,and suggest that PERK is a possible target for inhibiting excessive autophagy in cerebral ischemia/reperfusion injury.

关键词： apoptosis ATF4 autophagy C/EBP homologous protein cerebral ischemia/reperfusion injury eIF2α endoplasmic reticulum stress PERK

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Unveiling phosphofructokinase as the key target: an innovative strategy combining pull-down assays with optical imaging for snail-killing mechanism exploration

引用

Science China Chemistry 2025年

作者： Yuxun Lu Weisi Wang Le Yu Yuqiang Zhao Ying Chen Yunhai Guo Bingrong Luo Yuhua Liu Shizhu Li Liping Duan Ying Zhou Yunnan Characteristic Plant Extraction Laboratory College of Chemical Science and Technology Yunnan University College of Biological and Chemical Engineering Guangxi University of Science and Technology National Institute of Parasitic diseases Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research)NHC Key Laboratory of Parasite and Vector Biology WHO Collaborating Centre for Tropical Diseases National Center for International Research on Tropical Diseases School of Chemistry and Environment Yunnan Minzu University Dali Institute of Schistosomiasis control

The Pomacea canaliculata, also known as apple snail, is an invasive species in China, posing a serious threat to agricultural production and public health. To prevent its rampant infestation, we developed a highly efficient snail-killing lead compound, 1-(4-chlorophenyl)-3-(pyridin-3-yl)urea (PBQ). However, the killing mechanism of PBQ remains unclear. To elucidate this, two novel compounds, B-PBQ and J-PBQ, were developed through structural modification of PBQ and were used, through both protein pull-down assay and confocal laser scanning microscopy imaging, to identify and confirm that phosphofructokinase(PFK) in the apple snail is the primary target protein of PBQ. Further studies revealed that PBQ successfully inhibits the activity,content, and expression of PFK in apple snail, disrupting the glycolysis pathway and leading to abnormal energy *** disruptions impair the snail’s tolerance to low oxygen, resulting in its efficient eradication. These findings offer a promising direction for developing next-generation PFK-targeted snail-killing pesticides. Furthermore, the strategy of combining pull-down assays with optical imaging used in this work provides a novel approach for the exploration of mechanisms of other pesticide candidates.

关键词：

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Approach from the laboratory to the field: New strategies in the control of mosquito-borne infectious diseases

引用

Science China Life Sciences 2025年第1期 303-305页

作者： Ziqian Xu Lianpan Dai Jun Liu National Key Laboratory of Intelligent Tracking and Forecasting for Infectious diseases (NITFID) National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and Prevention CAS Key Laboratory of Pathogen Microbiology and Immunology Institute of MicrobiologyChinese Academy of Sciences

Vector-borne viruses, transmitted among humans and animal hosts through bites of vectors such as mosquitoes and ticks,pose a great and ongoing threat to public health and biosafety(Yang et al., 2022).Among them, dengue virus-induced epidemics, have dramatically increased worldwide in recent decades. The report released by the World Health Organization(WHO) at the end of 2023 showed that dengue epidemic globally surged in2023, with the number of reported cases nearing a historical peak.

关键词：

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Measles Virus IgG Avidity Assay for Use in Identification of Measles Vaccine Failures in Tianjin, China

引用

Biomedical and Environmental Sciences 2019年第11期32卷 804-811页

作者： DING Ya Xing MAO Nai Ying ZHANG Yan LEI Yue GAO Zhi Gang XU Wen Bo ZHANG Ying Department of Expanded Program Immunization Tianjin Center for Diseases Control and PreventionTianjin 300011China National Institute for Viral Disease control and prevention Chinese Center for Diseases Control and PreventionBeijing 102206China Department of Viral Laboratory Tianjin Center for Diseases Control and PreventionTiannin 300011China Institute for Infectious diseases control and prevention Tianjin Center for Diseases Control and PreventionTianjin 300011China

Objective To identify measles vaccine failures in Tianjin, China using a measles virus Ig G avidity *** The China Information System for Disease control and prevention(CISDCP) was used to collect information about measles cases and blood specimens in Tianjin from 2013 to 2015. Measlesspecific Ig M and Ig G antibodies were detected using Enzyme-Linked Immunosorbent Assay(ELISA).Avidity testing for measles Ig G was performed using a commercial enzyme immunoassay(EIA).Results A total of 284 confirmed measles cases were identified. Of this total, 262(92.25%) were in patients aged ≥ 20 years. High avidity was exhibited in 172(60.56%) cases, while 80(28.17%) cases demonstrated low avidity. High avidity was detected in only 21.43% of cases in patients aged 0.05) in clinical severity between high avidity and low avidity cases. However, regardless of vaccination status, clinical severity was significantly lower in high avidity cases(P < 0.001) than in low avidity cases. The percentages of positive measles Ig M results in high avidity and low avidity cases were 66.28% and 91.25%, respectively. Geometric Mean Concentration(GMC) was significantly lower in high avidity cases at 33.73 U/m L, compared to 166.07 U/m L in low avidity *** Low clinical severity and inconclusive Ig M antibody results are more likely in high avidity measles cases. Measles cases were more common in adults. Therefore, a further dose of vaccines should be recommended for 30–39 years in Tianjin.

关键词： Measles IgG avidity China Primary vaccine failures Secondary vaccine failures

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The frst avian infuenza A (H7N9) viral infection in humans in Zhejiang Province, China: a death report

引用

Frontiers of Medicine 2013年第3期7卷 333-344页

作者： Enfu Chen Fenjuan Wang Huakun Lv Yanjun Zhang Hua Ding Shelan Liu Jian Cai Li Xie Xiaoping Xu Chengliang Chai Haiyan Mao Jimin Sun Junfen Lin Zhao Yu Lianhong Li Zhiping Chen Shichang Xia Department of Infectious diseases Zhejiang Center for Disease Control and Prevention Department of Infectious diseases Xiaoshan District Center for Disease Control and Prevention Department of Infectious diseases Hangzhou Center for Disease Control and Prevention Department of Infectious diseases Jiande Center for Disease Control and Prevention

This study reports the frst death caused by a novel avian infuenza A(H7N9)virus in Zhejiang Province,*** patient had chronic hepatitis B and history of exposure to *** patient initially complained of diarrhea and infuenza-like symptoms on March 7 and 14 *** disease progressed to severe pneumonia,sustained hypoxia,and coagulation *** patient died on March 27 because of respiratory failure,multiple organ failure,and disseminated intravascular coagulation without oseltamivir *** H7N9 virus from Zhejiang is highly similar to isolates obtained from Shanghai,Jiangsu,Anhui,*** of hemagglutinin,neuramidinase,and matrix genes indicated that the isolates share the same avian origin,have low virulence,and are sensitive to oseltamivir,but are resistant to *** the isolate that caused the fatality exhibited substitution of Q226I in the HA gene,which indicates a potentially enhanced human *** secondary transmission rate was 1.6%(2/125).Only two health workers presented with infuenza-like symptoms,and they subsequently tested negative for H7N9 *** conclusion,underlying disease,late diagnosis,and untimely antiviral treatment are possible high-risk factors for infections and death caused by the lowpathogenicity avian infuenza A(H7N9).Person-to-person transmission of the H7N9 virus was not detected among close contacts,but such transmission should be investigated in the *** and enhancing surveillance will help in the early discovery and diagnosis of suspected cases,which will reduce the number of severe cases and deaths.

关键词： avian influenza A (H7N9) virus epidemiology contacts person-to-person transmission

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Psychoactive Substance Use and the Impact on the AIDS Epidemic Among the MSM Population:a Meta-Analysis—Worldwide,2014-2023

引用

China CDC weekly 2024年第42期6卷 1086-1092,I0001-I0006页

作者： Donghang Luo Jie Xu Wei Luo National center for AIDS/STD control&prevention Chinese Center for Diseases Control and PreventionBeijingChina

What is already known about this topic?There is a correlation between psychoactive substance use and heightened human immunodeficiency virus(HIV)susceptibility in men who have sex with men(MSM)populations,although the precise impact on transmission risk remains *** is added by this report?In MSM populations,rush poppers and amphetamines are widely used,with rates of 10.91%and 9.97%.In China,rush poppers usage is notably higher at 47.26%.For other regions and coutries,amphetamines(12.86%),rush poppers(3.62%),and GHB(3.34%)are most ***,17%of MSM use psychoactive substances,with higher HIV risk from amphetamines[Odds ratio(OR)3.06]compared to rush poppers(OR 1.51)and general substances(OR 1.76).What are the implications for public health practice?This study supports the idea that targeting specific regions and types of psychoactive substances for reduction among MSM populations can help decrease HIV transmission as well as promote prevention strategies.

关键词： prevention precise Impact

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Comparison of Xpert MTB/RIF, Real Amp, and CPA Tests in Detecting Mycobacterium tuberculosis

引用

Biomedical and Environmental Sciences 2019年第3期32卷 215-219页

作者： OU Xi Chao LI Hui LIU Dong Xin XIA Hui MA Xiao Guang WANG Shao Hua WANG Sheng Fen ZHU Yan Kun ZHAO Bing ZHAO Yan Lin National center for Tuberculosis control and prevention Chinese Center for Diseases Control and Prevention Henan Provincial center for Disease control and prevention

Today, tuberculosis (TB) remains a global public health threat associated with significantly high rates of morbidity and mortality. The World Health Organization's (WHO) Global Tuberculosis Report 2018[1] has reported that in 2017, 10.0 million people across the world had developed TB diseases that resulted in an estimated 1.6 million deaths, and 889, 000 people developed TB in China that led to 39, 000 TB-related deaths. Therefore, rapid and accurate detection of Mycobacterium tuberculosis (MTB) is important for initiating early treatment and reducing mortality. Traditional diagnostic methods for pulmonary TB incorporate chest radiography and sputum smear microscopy;however, several cases of tuberculosis go undiagnosed because of the low sensitivity of smear microscopy[2].

关键词： Comparison Detecting Mycobacterium tuberculosis Xpert MTB/RIF

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Multidimensional Analysis of Risk Factors Associated with Breast Cancer in Beijing,China:A Case-control Study

引用

Biomedical and Environmental Sciences 2020年第10期33卷 785-790页

作者： LI Ai Hua YE Yan CHEN Jun SUN Zhi Feng YUN Shui Ying TIAN Xing Kuan HU Zai Fang Sarah Robbins SCOTT YU Gui Xin HU Li WANG Zi Huan SUN Li Geng SHEN Zhuang Institute for Occupational Health Beijing Center for Diseases Prevention and Control&Center of Preventive Medical ResearchBeijing 100013China University of California Los Angeles 90024CaliforniaUSA Department of Occupational Health Dongcheng Center for Diseases Prevention and ControlBeijing 100009China Department of Occupational diseases and Environment Health Shijingshan Center for Diseases Prevention and ControlBeijing 100050China Department of Integrated Evaluation Mentougou Center for Diseases Prevention and ControlBeijing 102300China Department of Occupational Health Shunyi Center for Diseases Prevention and ControlBeijing 101300China The National center for AIDS control and prevention Chinese Center for Disease Control and PreventionBeijing 102206China

Breast cancer is the most common cancer among women worldwide[1].In 2018,2.09 million new breast cancer cases were identified globally,accounting for 11.6% of all cancers in that year[2].The breast cancer incidence in... 详细信息

关键词： Breast cancer Cancer

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Premature Stop Codon at Residue 101 within HIV-1 Rev Does Not Influence Viral Replication of Clade BC but Severely Reduces Viral Fitness of Clade B

引用

Virologica Sinica 2020年第2期35卷 181-190页

作者： Zheng Wang Xiaolin Ji Yanling Hao Kunxue Hong Liying Ma Dan Li Yiming Shao State Key Laboratory of Infectious diseases prevention and control Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesChinese Center for Disease Control and PreventionBeijing 102206China Division of Research of Virology and Immunology National Center for AIDS/STD Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China

HIV-1 Rev is an accessory protein that plays a key role in nuclear exportation,stabilization,and translation of the viral *** of HIV-1 clade BC often shows a truncation of 16 AAs due to a premature stop codon at residue *** stop codon presents the highest frequency in clade BC and the lowest frequency in clade *** order to discover the potential biological effect of this truncation on Rev activity and virus replication of clade BC,we constructed Rev expression vectors of clade BC with or without 16 AAs within C-terminal separately,and replaced the stop codon by Q in a CRF07_BC infectious *** found that 16 AAs truncation had no effect on expression and activity of Rev in clade ***,the mutation from the stop codon to Q had no effect on virus replication of clade ***,to investigate the effect of this truncation on Rev activity and replication capacity of clade B,Rev expression vectors of clade B carrying or lacking 16 AAs in C-terminal were constructed respectively,and residue Q at position 101 within Rev was substituted by the stop codon in a clade B infectious *** was found that 16 AAs truncation significantly down-regulated Rev expression and impaired clade B Rev ***,a Q-to-stop codon substitution within Rev significantly reduced viral replication fitness of clade *** results indicate that the premature stop codon at residue 101 within Rev exerts diverse impact on viral replication among different HIV-1 clades.

关键词： HIV-1 CRF_BC Rev Premature stop codon Replication

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Prevalence of antifolate drug resistance markers in Plasmodium vivax in China

引用

Frontiers of Medicine 2022年第1期16卷 83-92页

作者： Fang Huang Yanwen Cui He Yan Hui Liu Xiangrui Guo Guangze Wang Shuisen Zhou Zhigui Xia National Institute of Parasitic diseases Chinese Center for Disease Control and PreventionChinese Center for Tropical Diseases ResearchWHO Collaborating Center for Tropical DiseasesNational Centre for International Research on Tropical DiseasesNHC Key Laboratory of Parasite and Vector Biology(National Institute of Parasitic DiseasesChinese Center for Disease Control and Prevention)Shanghai 200025China Yunnan Institute of Parasitic diseases Puer 665000China Yingjiang County for Disease control and prevention Yingjiang 679300China Hainan center for Disease control&prevention Haikou 570203China

The dihydrofolate reductase(dhfr)and dihydropteroate synthetase(dhps)genes of Plasmodium vivax,as antifolate resistance-associated genes were used for drug resistance surveillance.A total of 375 *** isolates collected from different geographical locations in China in 2009–2019 were used to sequence Pvdhfr and *** majority of the isolates harbored a mutant type allele for Pvdhfr(94.5%)and Pvdhps(68.2%).The most predominant point mutations were S117T/N(77.7%)in Pvdhfr and A383G(66.8%)in *** acid changes were identified at nine residues in Pvdhfr.A quadruple-mutant haplotype at 57,58,61,and 117 was the most frequent(57.4%)among 16 distinct Pvdhfr *** in Pvdhps were detected at six codons,and the double-mutant A383G/A553G was the most prevalent(39.3%).Pvdhfr exhibited a higher mutation prevalence and greater diversity than Pvdhps in *** isolates from Yunnan carried multiple mutant haplotypes,while the majority of samples from temperate regions and Hainan Island harbored the wild type or single mutant *** study indicated that the antifolate resistance levels of *** parasites were different across China and molecular markers could be used to rapidly monitor drug *** provided evidence for updating national drug policy and treatment guidelines.

关键词： drug resistance antifolates molecular markers Plasmodium vivax China

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：