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Cloning and Expression of Anthocyanin Biosynthesis Related Gene RrMYB6 in Rosa rugosa

Agricultural Sciences 2018年第3期9卷 374-383页

作者： Kai Zou Yang Wang Mingyuan Zhao Lanyong Zhao Zongda xu Forestry College of Shandong Agricultural University Tai’an China

R2R3-MYB transcription factor plays an important role in plant anthocyanin synthesis. Based on the transcriptional database of Rosa rugosa, one MYB transcription factor related to floral color, RrMYB6, was cloned. By using bioinformatics analysis method, cloning MYB gene and analyzing its function in anthocyanin biosynthesis regulation, we hope to lay a solid foundation for new color variety breeding of R. rugosa. Using the R. rugosa “Zi zhi” as the material, we obtained the total length of cDNA of RrMYB6 by RT-PCR and RACE. By analyzing its bioinformatics, we found that the formula of the protein was C1491H2368N452O470S17, molecular weight was 34690.97 Da, the theoretical pI was 8.74. In addition, it belonged to unstable protein with an unstable index at 50.59, and it was also a hydrophilic protein with the total average hydrophobic index at -0.847. In the secondary structure of RrMYB6 protein, the Alpha helix accounted for 32.35%, random coil was 47.39%, extended strand was 11.11%, and beta turn was 9.15%. The sequence analysis showed that RrMYB6 had a typical R2R3-MYB domain and bHLH binding domain, and it also had an N1, C1, C2 inhibitory motif, belonging to the Sg4 subfamily MYB protein. What’s more, evolutionary analysis indicated that the RrMYB6 protein was closely related with the MYB protein in Rosacea family, while it was far from those in other families. The expression analysis showed that RrMYB6 protein decreased with the color of petals deeping, and its expression was the lowest in the petals while the highest in stamens. According to the above results, it was speculated that RrMYB6 was involved in regulating the anthocyanin synthesis of R. rugosa, which belonged to negative regulatory mechanism.

关键词： Rosa rugosa Anthocyanin R2R3-MYB Gene Expression

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Thermal Deformation Behavior and Processing Map of a Novel CrFeNiSi_(0.15)Medium Entropy Alloy

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Acta Metallurgica Sinica(English Letters) 2023年第11期36卷 1870-1882页

作者： Hongbin Zhang Kang Chen Zhongwei Wang Haiping Zhou Chengcheng Shi Shengxue Qin Jie Liu Tingjun Lv Jian xu College of Mechanical and Electronic Engineering Shandong University of Science and TechnologyQingdao266590China School of Mechanical Engineering Shandong University of TechnologyZibo255000China Zibo Yuan xu Metallurgical Machinery Co LtdZibo255120China

The thermal deformation behavior of a novel CrFeNiSi_(0.15)medium entropy alloy(MEA)was studied via isothermal compression experiments,with the processing parameter range of 900–1200℃and 0.001–1 s^(−1).According to experimental data,the modified constitutive equation had been obtained,which precisely predicted the flow behavior of CrFeNiSi_(0.15)MEA during thermal *** the same time,the processing map was established on the basis of the dynamic material model(DMM)*** to the map,the optimal processing parameters were determined at 1130–1200℃/0.06–1 s−1,under which the power dissipation efficiency could reach above 34%.The peak efficiency was above 38%,which occurred at 1200℃/1 s^(−1).In such parameter,complete dynamic recrystallization(DRX)also *** flow instability of CrFeNiSi_(0.15)MEA was estimated to occur at 900–985℃/0.12–1 s^(−1),which was shown as grain boundaries ***,both the continuous DRX(CDRX)and discontinuous DRX(DDRX)occurred simultaneously during thermal ***,some twins were also newly formed during DRX process,most of which were primary *** occurrence of twinning was beneficial to promote the development of DRX behavior.

关键词： Medium entropy alloys Thermal deformation behavior Constitutive model Processing maps

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Processing of metastable beta titanium alloy:Comprehensive study on deformation behaviour and exceptional microstructure variation mechanisms

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Journal of Materials Science & Technology 2022年第31期126卷 22-43页

作者： Qinyang Zhao Leandro Bolzoni Yongnan Chen Yiku xu Rob Torrens Fei Yang School of Materials Science and Engineering Chang’an UniversityXi’an 710064China Waikato Centre for Advanced Materials and Manufacturing School of EngineeringThe University of WaikatoHamilton 3240New Zealand

Thermomechanical processing(TMP)is especially crucial for metastableβtitanium alloys,which has received significant attention in the community for a long *** this contribution,the processing-responding behaviour including microstructure evolution process,texture variation mechanism,and un-derlying deformation process of powder metallurgy Ti-5553 alloy in a wide processing parameter range was comprehensively *** physical simulation was performed on the alloy at temper-atures ranging from 800℃ to 1100℃,and strain rates between 0.001 s^(−1) and 10 s^(−1),to varied defor-mation degrees of 20%-80%height *** was found that the processing parameters(***-ature,strain rate,and deformation degree)are influential on the deformation process and resultant *** microstructural evolution processes forβphase including flow localization,dynamic recovery,dynamic recrystallization,and grain coarsening are activated in different processing domains,while different evolution mechanisms forαphase including dynamic precipitation,phase separation,dy-namic coarsening,and mechanical shearing also play their roles under different processing *** particular,four exceptional evolution mechanisms ofαprecipitation which have not been previously reported in titanium alloys were discovered and clearly demonstrated,more specifically,they are multi-interior twinning,internal compositing,layered coarsening and selective diffusion-actuated *** the establishment of comprehensive microstructural evolution mechanism maps,the guidance for precise processing and the knowledge reserve extension for deformation process of metastableβtita-nium alloys can be effectively achieved.

关键词： Metastableβtitanium alloy Powder metallurgy TMP Microstructure evolution Texture variation

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Integrative analysis of the transcriptome and metabolome reveals the response mechanism to tomato spotted wilt virus

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Horticultural Plant Journal(园艺学报:英文版) 2023年第5期9卷 958-970页

作者： Junheng Lv Minghua Deng Zuosen Li Haishan Zhu Ziran Wang Yanling Yue Zhengan Yang Junqiang xu Shurui Jiang Wei Zhao Jing Li Kai Zhao College of Horticulture and Landscape Yunnan Agricultural UniversityKunmingYunnan 650201China Dian-tai Engineering Research Center for Characteristic Agriculture Industrialization of Yunnan Province Yunnan Agricultural UniversityKunmingYunnan 650201China

Tomato spotted wilt virus(TSWV)is an important virus that has rapidly spread throughout the *** seriously hinders the production of tomato(Solanum lycopersicum)and other *** order to discover more new genes and metabolites related to TSWV resistance in tomato plants,the genes and metabolites related to the resistance of tomato plants inoculated with TSWV were identified and studied *** tomato TSWV-resistance line YNAU335(335)and TSWV-susceptible lines NO5 and 96172I(961)were used as the transcriptome and metabolome research *** and metabolomic techniques were used to analyze the gene and metabolite response mechanisms to TSWV inoculation.A total of 3566,2951,and 2674 differentially expressed genes(DEGs)were identified in lines 335,NO5,and961,***,208,228,and 273 differentially accumulated metabolites(DAMs)were identified in lines 335,NO5,and 961,*** line 335,the number of DEGs was the highest,but the number of DAMs was ***,903 DEGs and 94 DAMs were common to the response to TSWV in the three inbred *** 903 DEGs and 94 DAMs were mainly enriched in the plant hormone signal transduction and flavonoid synthesis *** addition,many nucleotide-binding site-leucine-rich repeat genes and transcription factors were found that might be involved in the TSWV *** results provide new insights into TSWV resistance mechanisms.

关键词： Tomato plant Tomato spotted wilt virus(TSWV) Flavonoid synthesis Plant hormone signal transduction Transcriptome and metabolome

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Cloning and Expression Analysis of RrMYB113 Gene Related to Anthocyanin Biosynthesis in Rosa rugose

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American Journal of Plant Sciences 2018年第4期9卷 701-710页

作者： Kai Zou Yang Wang Mingyuan Zhao Lanyong Zhao Zongda xu Forestry College of Shandong Agricultural University Tai’an China

Anthocyanin is one of water-soluble natural pigments widely existing in flowers, fruits, stems, leaves and seeds of plants, and it is the major factor conferring pink or red to the petals of Rosa rugose. MYB TFs play an important role in the anthocyanin synthesis in plants. This work aimed to clone the MYB gene related to anthocyanin synthesis in the petals of Rosa rugose, and explore the relationship between them to lay a good foundation for gene engineering improvement of R. rugose. Based on the transcriptional data, a full-length cDNA sequence of MYB Gene, RrMYB113 (GenBank accession Nos MG720012), was cloned at the first time from the petals of Rosa rugose “Zi zhi” with RT-PCR and RACE methods. The full-length cDNA is 885 bp with an open reading frame of 654 bp, encoding 216 amino acids. The derived RrMYB113 protein has a molecular weight of 25,297.64 Da, a calculated pI of 9.61, a R2R3-MYB domain and bHLH binding domain, and it also has the signature motifs ((A/S/G)NDV and KPRPR(T/S)), thus belonging to Sg6 R2R3-MYB subfamily. In the secondary structure of RrMYB113 protein, there is 37.04% α-helix, 39.81% random coil, 14.81% extended peptide chain, and 8.33% β-corner. There is no transmembrane domain and no signal peptide cleavage site, seventeen Ser phosphorylation sites, fifteen Thr phosphorylation sites, four Tyr phosphorylation sites, and no O-glycosylation sites. The expression of RrMYB113 increased with the color deepening in petals, and it expressed at a higher level in petals than in other tissues of R. rugose “Zi zhi”. These results are meaningful to reveal that RrMYB113 might be an important regulator in anthocyanin biosynthesis and coloration in the petals of R. rugose.

关键词： Rosa rugose Anthocyanin R2R3-MYB Gene Expression

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Target-oriented Q-compensated reverse-time migration by using optimized pure-mode wave equation in anisotropic media

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Petroleum Science 2023年第2期20卷 866-878页

作者： Shi-Gang xu Qian-Zong Bao Zhi-Ming Ren chang'an University School of Geological Engineering and GeomaticsDepartment of GeophysicsXi'anShaanxi710064China National Engineering Research Center of Offshore Oil and Gas Exploration Beijing100028China

Research on seismic anisotropy and attenuation plays a significant role in exploration geophysics. To enhance the imaging quality for complicated structures, we develop several effective improvements for anisotropic attenuation effects in reverse-time migration (Q-RTM) on surface and vertical seismic profiling (VSP) acquisition geometries. First, to suppress pseudo-shear wave artifact and numerical instability of the commonly used anisotropic pseudo-acoustic wave equations, an optimized pure P-wave dispersion relation is derived and the corresponding pure-mode wave equation is solved by combining the finite-difference and Possion methods. Second, a simplified anisotropic pure-mode visco-acoustic wave equation (PVAWE) based on standard linear solid model is established. Third, a time-dispersion correlation strategy is applied to improve the modeling accuracy. Fourth, we extend a target-oriented scheme to anisotropic attenuated modeling and imaging. Instead of the conventional wavefield modeling and RTM, the proposed approach can extract available wavefield information near the target regions and produce high imaging resolution for target structures. Last, both anisotropic surface and VSP Q-RTMs are executed by combining optimized PVAWE, time-dispersion correlation and target-oriented algorithm. Modeling examples demonstrate the advantages of our schemes. Moreover, our modified Q-compensated imaging workflow can be regarded as a supplement to the classical anisotropic RTM.

关键词： Anisotropy Attenuation Reverse-time migration Wave equation Optimized algorithm Target-oriented

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Forward-backward asymmetries inΛ_(b)→Λl^(+)l^(-)in the Bethe-Salpeter equation approach

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Chinese Physics C 2022年第9期46卷 94-102页

作者： Liang-Liang Liu Su-Jun Cui Jing xu Xin-Heng Guo College of Physics and information engineering Shanxi Normal UniversityTaiyuan 030031China Department of Physics Yan-Tai UniversityYantai 264005China College of Nuclear Science and Technology Beijing Normal UniversityBeijing 100875China

Using the Bethe-Salpeter equation(BSE),we investigate the forward-backward asymmetries(AFB)inΛb→Λl^(+)l^(-)(l=e,μ,τ)in the quark-diquark *** approach provides precise form factors that are different from those of quantum chromodynamics(QCD)sum *** calculate the rare decay form factors forΛb→Λl^(+)l^(-)b and investigate the(integrated)forward-backward asymmetries in these decay *** observe the integrated A l FB,ˉA l FB(Λb→Λe^(+)e^(-)=-0.1371,ˉA l FB(Λb→Λμ^(+)μ^(-))=-0.1376,andˉA l FB(Λb→Λτ^(+)τ^(-))=-0.1053;the hadron side asymmetriesˉA h FB(Λb→Λμ^(+)μ^(-))=-0.2315;the lepton-hadron side asymmetriesˉA lh FB(Λb→Λμ^(+)μ^(-))^(-)0.0827;and the longitudinal polarization fractionsˉF L(Λb→Λμ^(+)μ^(-))^(-)0.5681.

关键词： rare decay Bethe-Salpeter equation heavy flavor physics

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Effects of Supplementation with Saccharomyces cerevisiaeand Its Fermentation Products on Production Performance and Its Mechanism in Perinatal Dairy Cows

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Advances in Bioscience and Biotechnology 2021年第7期12卷 193-212页

作者： xueyan Lin Ke Li Lin Ju xu Hao Yue Jiang Qiuling Hou Zhiyong Hu Yun Wang Zhonghua Wang College of Animal Science Shandong Agricultural University Tai’an China

The aim of this study was to investigate the effects of Saccharomyces cerevisiae and its fermentation products on performance, blood hormone levels and rumen floral composition in peripartum dairy cows. Sixty perinatal cows were selected and allocated to two groups according to parity and expected date of delivery. Each group was supplemented with Saccharomyces cerevisiae and its fermentation product 0 or 100 g. The results showed that Saccharomyces cerevisiae and its fermentation products could significantly increase the feed intake of peripartum dairy cows (P β-hydroxybutyrate (P = 0.01), reducing the insulin content (P = 0.02). Saccharomyces cerevisiae reduced the abundance of rumen microbes in peripartum dairy cows but had no effect on rumen microbial diversity. Compared with the control group, the supplemented group showed reductions in the abundance of genera Bacillus (P = 0.03), Butyrivibrio (P = 0.01),

关键词： Saccharomyces cerevisiae Perinatal Period Dairy Cows Production Performance Mechanism

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The Multicast Scheme of ATM Networks for Multimedia Transmissions Based on SD Channel

The Multicast Scheme of ATM Networks for Multimedia Transmis...

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2012年计算机应用与系统建模国际会议

作者： Wang Ting Hao Jianzhong Zhuo Li Wang Shuling Wei Yan xu Zhou Air Force College

In this paper, we propose a bi-directional multipoint-to-multipoint multicast scheme, a SD channel-based Multicast with Round robin Access (SDRAM), for ATM networks, which uses a single tree for a multicast group consisting of multiple participants that are either senders, receivers, or a mix of both. We first discuss why the resequencer model will not be suitable for multimedia traffics, and then propose the SDRAM scheme to solve the problems, and finally compare our scheme with the resequencer model through simulation. Results show the mean queuing delays and mean inter-PDU delays of our scheme are not sensitive to mean PDU size while the mean queuing delays and mean inter-PDU delays of the resequencer scheme are very sensitive to mean PDU size. Index Terms-ATM, multicast, resequencer, round robin, shared tree.

关键词： TM Networks Multicast SD Channel PDU Delay

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Uncover the mystery of pleiotropic effects of PROG1 during rice domestication

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Molecular Plant 2023年第11期16卷 1722-1723页

作者： Wenguang Wang Songtao Gui Yonghong Wang National Key Laboratory of Wheat Improvement College of Life SciencesShandong Agricultural UniversityTai'an 271018China Institute of Genetics and Developmental Biology Chinese Academy of SciencesBeijing 100101China

Rice is one of the earliest domesticated crops,and the plant and panicle architecture are critical domesticated traits that greatly affect yield(xu and Sun,2021).Previous studies revealed that PROSTRATE GROWTH 1(PROG1)regulates tiller angle,tiller number,and panicle architecture during rice domestication(Jin et al.,2008;Tan et al.,2008).However,the detailed molecular mechanisms through which PROG1 controls plant and panicle architecture have not yet been well documented.

关键词： crops panicle domestica

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