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Frontiers in Biology 2016年第1期11卷 28-31页

作者： Vadim V. Davydov Evgenya R. Grabovetskaya Amjad Hamdallah chair of Biochemistry State Academican LP.Pavlov medical university Ryazan 390026 Russia chair of medical chemistry kharkov national medical university Kharkov 61022 Ukraine chair of Biochemistry EN. Karazin kharkov national university Kharkov 61077 Ukraine

The purpose of this study was a comparative investigation of activity of aldehyde scavenger enzymes in mitochondrial fraction of a thigh muscle in intact and immobilized rats of different ages. It has been shown that 12- month-old （adult） rats have high basal levels of aldehyde dehydrogenase, aldehyde reductase and glutathione transferase activity in mitochondrial fraction of thigh muscle. Aldehyde dehydrogenase activity increases during immobilization stress in adult rats. This change promote to enhance the effectiveness of utilization of carbonyl products of free radical oxidation in mitochondria of skeletal muscle of 12-month-old rats during stress. Immobilization of old and pubertal rats is accompanied by metabolic preconditions leading to accumulation of endogenous aldehydes in mitochondria, and, as a result, to the injury of muscular fibers and intensification of sarcopenia manifestations.

关键词： aldehydes mitochondria muscle immobilization stress aging

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Frontiers in Biology 2014年第4期9卷 283-286页

作者： Vadim V. DAVYDOV Amjad HAMDALLAH Evgenya R. GRABOVETSKAYA Laboratory of Age Biochemistry State Institution institute of Children and Adolescent Health NAMS of Ukraine Kharkov 61154 Ukraine chair of biochemistry V.N. Karazin kharkov national university Kharkov 61077 Ukraine chair of medical chemistry kharkov national medical university Kharkov 61022 Ukraine

The age-dependent peculiarities of stimulation of free radical processes in subcellular fractions of skeletal muscle of rats subjected to long-term immobilization stress were studied in order to improve knowledge about changes of muscular tissue during ontogenesis. It is found that adult animals do not show accumulation of proteins carbonyls, TBA-reactive substances, and Schiff bases in subcellular fractions of the thigh muscle when immobUized. Long-term immobilization causes apparent manifestation of oxidative stress only in mitochondrial fraction in pubertal rats. Mitochondrial oxidative stress defense systems are sufficiently effective, however, direction of pathways of free radical oxidation carbonyl products catabolism alters in the cytoplasm of myocytes in old rats under long-term immobilization conditions.

关键词： ontogenesis stress skeletal muscle free radical oxidation

Physiological significance of oxidative stress and its role in adaptation of the human body to deleterious factors

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Frontiers in Biology 2018年第1期13卷 19-27页

作者： Vadim V. Davydov Alexander V. Shestopalov Evgenya R. Grabovetskaya chair of biochemistry and molecular biology Pirogov Russian national Research medical university Moscow 117997 Russia chair of biochemistry V.N. Karazin kharkov national university Ukraine Kharkov 61077 Ukraine

BACKGROUND： Oxidative stress is an extremely widespread condition manifested in an increased rate of free-radical processes and accumulation of reactive oxygen species （ROS） in the tissues. It appears in different physiologic states and pathological processes accompanied by stimulation of the sympathetic adrenal system or tissue hypoxia or under stress. However, until now, there is still no clarity on the issue of the significance of oxidative stress in the development of adaptation processes in the organism. OBJECTIVE： In the present work we will review the most recent finding about physiologic role of oxidative stress and its participation in adaptation of an organism to effect of different adverse factors. METHODS： A systematic literature search was performed using the Pubmed search engine. Studies published over past 18 years, i.e. between 1998 and 2015 were considered for review. Followed keywords were used：＂oxidative stress,＂＂free radical oxidation,＂＂ROS,＂＂endogenous aldehydes,＂＂adaptation.＂ RESULTS： The article cites arguments supporting the notion that oxidative stress serves as a nonspecific link in the adaptation of the human body to the effects of injurious factors. Oxidative stress exerts regulatory effects by changing the redox state of the cell. Oxidative stress affects on various intracellular proteins containing cysteine residues, e.g., enzymes, chaperones, and transcription factors, etc. For this reason, the use of antioxidants for the treatment and prophylaxis of a wide range of diseases is not recommended. CONCLUSION： Further investigation is needed in this field. The most attention should be paid to careful experimental verification aimed at quantitative assessment of the ROS level in tissues under oxidative stress, as well as at the study of possibility of enhancing the catabolism of free radical oxidation carbonyl products in order to prevent tissue damage under oxidative stress.

关键词： oxidative stress free radical oxidation ROS adaptation endogenous aldehydes

Synthesis and Crystal Structure of 12-(2,4-Dichlorophenyl)-7,8,9,10,11,12-hexahydrobenzo[f]pyrimido [4,5-b]quinoline-9,11-dione DMF Solvate

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Chinese Journal of Structural chemistry 2010年第3期29卷 335-340页

作者：王香善李庆吴健蓉张梅梅 School of chemistry and Chemical Engineering Xuzhou Normal University Key Laboratory of Biotechnology for medical Plant Xuzhou Normal University

The title compound 12-（2,4-dichlorophenyl）-7,8,9,10,11,12-hexahydrobenzo-[f]pyrimido[4,5-b]quinoline-9,11-dione DMF solvate 1（C24H20Cl2N4O3,Mr=483.34） was synthesized and *** crystal belongs to the monoclinic system,space group P21/n with a=6.4798（16）,b=20.595（5）,c=16.723（4）,β=95.267（7）o,Z=4,V=2222.3（9）3,Dc=1.445 g/cm3,μ（MoKα）=0.328 mm-1,F（000）=1000,the final R=0.0530 and wR=0.1388 for 3484 observed reflections（I2σ（I））.X-ray analysis reveals that the central 1,4-dihydropyridine ring is slightly distorted,adopting a half-chair conformation,which is obviously different from the boat conformations of other similar *** addition,the classical hydrogen bonds of N（2）-H（2A）…O（2） are presented in the crystals,and link the adjacent molecules to form *** O（3） in the DMF solvent forms two hydrogen bonds of O（3）…H（1H）-N（1） and O（3）…H（3H）-N（3） with a pyrimidoquinoline moiety,forming an interesting half-chair-like *** hydrogen bonds link the molecules to form dimmers,while intermolecular π-π interaction connects the above-mentioned dimmers to yield a one-dimensional structure.

关键词： crystal structure pyrimidoquinoline synthesis

Interlayer-type Crystal Structure of N-(1-Adamantyl)urea

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Chinese Journal of Structural chemistry 2006年第4期25卷 473-476页

作者：杨云霞张锦楠宋晓红李奇 Department of chemistry Beijing Normal University Beijing 100875 ChinaDepartment of Chemistry The Northwest Normal University Lanzhou 730030 China School of Pharmaceutical Sciences Capital University of Medical Sciences Beijing 100054 China Department of chemistry Beijing Normal University Beijing 100875 China

The crystal structure of N-（1-adamantyl）urea （tricyclo[3,3,1, 1^3.7]decan-1-urea, C11H18N2O） has been determined by X-ray diffraction. The crystal belongs to the monoclinic system, space group P2（1/c） with a = 13.070（2）, b = 8.942（1）, c = 9.390（1） A^°, β= 109.819（8）°, V= 1032.5（2） A^°3, Mr= 194.27, Z = 4, Dc = 1.250 g/cm^3,μ = 0.081 mm^-1, F（000） = 424, R= 0.0427 and wR = 0.1076 for 1105 observed reflections with I〉 2σ（I）. The structure of the title compound consists of an adamantly cage which is a stable tricyclo structure with chair conformation and the substituted urea forms the interlayer-type crystal structure via hydrogen bonds between the molecules.

关键词： N-(1-adamantyl)urea crystal structure hydrogen bond

Synthesis and Crystal Structure of Monocarbonyl Analogs of Curcumin

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Chinese Journal of Structural chemistry 2012年第4期31卷 491-498页

作者：赵承光陈小燕胡杰张丽赵云洁杨树林梁广 School of Pharmacy Wenzhou Medical College Institute of Biotechnology Nanjing University of Science and Technology The First Affiliated Hospital Wenzhou Medical College

Two monocarbonyl analogs of curcumin containing bis（2,6-difluorobenzyl） were synthesized,and their molecular structures were characterized by 1H NMR,ESI-MS,and X-ray *** this paper,the crystal structures of these two analogs（A75 and C75） are described and compared with their bis（2-fluorobenzyl） analogues as well as with the available data of *** cyclopentane rings in A75 have an envelope conformation,while the cyclohexane ring in C75 has a chair *** compounds are symmetrical and the central structure of A75 and C75 connects with two benzylidene rings through E,E-oriented double bonding.

关键词： bis(2 6-difluorobenzyl) X-ray crystallography mono-carbonyl analogues of curcumincurcumin

Azacarbazole n-3 and n-6 polyunsaturated fatty acids ethyl esters nanoemulsion with enhanced efficacy against Plasmodium falciparum

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Bioactive Materials 2021年第4期6卷 1163-1174页

作者： Anna Jaromin Silvia Parapini Nicoletta Basilico Magdalena Zaremba-Czogalla Agnieszka Lewi´nska Agnieszka Zag´orska Maria Walczak Bozena Tyliszczak Aleksandra Grzeszczak MarcinŁukaszewicz Łukasz Kaczmarek Jerzy Gubernator Department of Lipids and Liposomes Faculty of BiotechnologyUniversity of WroclawWroclawPoland Dipartimento di Scienze Biomediche per la Salute Universit`a degli Studi di MilanoMilanItaly Dipartimento di Scienze Biomediche Chirurgiche e OdontoiatricheUniversit`a degli Studi di MilanoMilanItaly Faculty of chemistry University of WroclawWroclawPoland Department of Medicinal chemistry Jagiellonian University Medical CollegeCracowPoland chair and Department of Toxicology Jagiellonian University Medical CollegeFaculty of PharmacyCracowPoland Instytute of Materials Science Cracow University of TechnologyCracowPoland Department of Biotransformation Faculty of BiotechnologyUniversity of WroclawWroclawPoland Pharmaceutical Research Institute WarsawPoland

Alternative therapies are necessary for the treatment of malaria due to emerging drug ***,many promising antimalarial compounds have poor water solubility and suffer from the lack of suitable delivery systems,which seriously limits their *** address this problem,we synthesized a series of azacarbazoles that were evaluated for antimalarial activity against D10(chloroquine-sensitive)and W2(chloroquine-resistant)strains of *** most active compound,9H-3-azacarbazole(3),was encapsulated in a novel o/w nanoemulsion consisting of ethyl esters of polyunsaturated fatty acids n-3 and n-6 obtained from flax oil as the oil phase,Smix(Tween 80 and Transcutol HP)and *** formulation was further analyzed using transmission electron microscopy,dynamic light scattering and in vitro and in vivo *** was shown that droplets of the 3-loaded nanosystem were spherical,with satisfactory stability,without cytotoxicity towards fibroblasts and intestinal cell lines at concentrations corresponding to twice the IC50 for ***,the nanoemulsion with this type of oil phase was internalized by Caco-2 ***,pharmacokinetics demonstrated rapid absorption of compound 3(tmax=5.0 min)after intragastric administration of 3-encapsulated nanoemulsion at a dose of 0.02 mg/kg in mice,with penetration of compound 3 to deep *** 3-encapsulated nanoemulsion was found to be 2.8 and 4.2 times more effective in inhibiting the D10 and W2 strains of the parasite,respectively,compared to non-encapsulated *** findings support a role for novel o/w nanoemulsions as delivery vehicles for antimalarial drugs.

关键词： Azacarbazoles Flax oil n-3 and n-6 polyunsaturated fatty acids ethyl esters Nanoemulsion P.falciparum Malaria

Post-translational O-GlcNAcylation is essential for nuclear pore integrity and maintenance of the pore selectivity filter

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Journal of Molecular Cell Biology 2016年第1期8卷 2-16页

作者： Yanping Zhu Ta-Wei Liu Zarina Madden Scott A.Yuzwa Kelsey Murray Samy Cecioni Natasha Zachara David J.Vocadlo Department of chemistry Simon Fraser UniversityBurnabyBritish Columbia V5A 1S6Canada Department of Molecular Biology and Biochemistry Simon Fraser UniversityBurnabyBritish Columbia V5A 1S6Canada Department of Biological chemistry Johns Hopkins University Medical SchoolBaltimoreMD 21205USA

O-glycosylation of the nuclear pore complex(NPC)by O-linked N-acetylglucosamine(O-GlcNAc)is conserved within *** nucleoporins(Nups)comprising the NPC are constitutively O-GlcNAcylated,but the functional role of this modification remains *** loss ofO-GlcNAc,induced by either inhibition ofO-GlcNAc transferase(OGT)or deletion of the gene encoding OGT,leads to decreased cellular levels of a number of natively O-GlcNAcylated *** of O-GlcNAc enables increased ubiquitination of these Nups and their increased proteasomal *** decreased half-life of these deglycosylated Nups manifests in their gradual loss from the NPC and a downstream malfunction of the nuclear pore selective permeability barrier in both dividing and post-mitotic *** findings define a critical role of O-GlcNAc modification of the NPC in maintaining its composition and the function of the selectivity *** results implicate NPC glycosylation as a regulator of NPC function and reveal the role of conserved glycosylation of the NPC among metazoans.

关键词： post-translational modiﬁcation O-GlcNAcylation nuclear pore complex protein stability ubiquitination nucleoporin glycosylation

3D RNA nanocage for encapsulation and shielding of hydrophobic biomolecules to improve the in vivo biodistribution

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Nano Research 2020年第12期13卷 3241-3247页

作者： Congcong Xu Kaiming Zhang Hongran Yin Zhefeng Li Alexey Krasnoslobodtsev Zhen Zheng Zhouxiang Ji Sijin Guo Shanshan Li Wah Chiu Peixuan Guo Center for RNA Nanobiotechnology and Nanomedicine College of PharmacyDivision of Pharmaceutics and Pharmaceutical ChemistryCollege of MedicineDorothy M.Davis Heart and Lung Research InstituteJames Comprehensive Cancer CenterThe Ohio State UniversityColumbusOH43210USA Department of Bioengineering James H.Clark CenterStanford UniversityStanfordCA94305USA Department of Physics University of Nebraska at OmahaOmahaNE68182USA Nanoimaging Core Facility Office of Vice-Chancellor for ResearchUniversity of Nebraska Medical CenterOmahaNE68198USA SLAC national Accelerator Laboratory Stanford UniversityMenlo ParkCA94025USA

Ribonucleic acid(RNA)nanotechnology platforms have the potential of harboring therapeutics for in vivo delivery in disease ***,the nonspecific interaction between the harbored hydrophobic drugs and cells or other components before reaching the diseased site has been an obstacle in drug *** we report an encapsulation strategy to prevent such nonspecific hydrophobic interactions in vitro and in vivo based on a self-assembled three-dimensional(3D)RNA *** placing an RNA three-way junction(3WJ)in the cavity of the nanocage,the conjugated hydrophobic molecules were specifically positioned within the nanocage,preventing their exposure to the biological *** assembly of the nanocages was characterized by native polyacrylamide gel electrophoresis(PAGE),atomic force microscopy(AFM),and cryogenic electron microscopy(cryo-EM)*** stealth effect of the nanocage for hydrophobic molecules in vitro was evaluated by gel electrophoresis,flow cytometry,and confocal *** in vivo sheathing effect of the nanocage for hydrophobic molecules was assessed by biodistribution profiling in *** RNA nanocages with hydrophobic biomolecules underwent faster clearance in liver and spleen in comparison to their ***,this encapsulation strategy holds promise for in vivo delivery of hydrophobic drugs for disease treatment.

关键词： ribonucleic acid(RNA)nanocage three-way junction(3WJ) encapsulation hydrophobic biomolecule RNA nanotechnology

Surface processing for iron-based degradable alloys:A preliminary study on the importance of acid pickling

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Bioactive Materials 2022年第5期7卷 166-180页

作者： Leticia Marin de Andrade Carlo Paternoster Pascale Chevallier Sofia Gambaro Paolo Mengucci Diego Mantovani Laboratory for Biomaterials and Bioengineering Canada Research Chair I in Biomaterials and Bioengineering for the Innovation in SurgeryDepartment of Min-Met-Materials EngineeringResearch Center of CHU de QuebecDivision of Regenerative MedicineLaval UniversityQuebec CityQCG1V 0A6Canada national Research Council Institute of Condensed Matter Chemistry and Technologies for Energy CNR-ICMATEGenoa16149Italy Polytechnic university of Marche Ancona60121Italy

The formation of a heterogeneous oxidized layer,also called scale,on metallic surfaces is widely recognized as a rapid manufacturing event for metals and their *** or total removal of the scale represents a mandatory integrated step for the industrial fabrication processes of medical *** biodegradable metals,acid pickling has already been reported as a preliminary surface preparation given further processes,such as ***,biodegradable medical prototypes presented discrepancies concerning acid pickling studies based on samples with less complex geometry(e.g.,non-uniform scale removal and rougher surface).Indeed,this translational knowledge lacks a detailed investigation on this process,deep characterization of treated surfaces properties,as well as a comprehensive discussion of the involved *** this study,the effects of different acidic media(HCl,HNO_(3),H_(3)PO_(4),CH_(3)COOH,H_(2)SO_(4) and HF),maintained at different temperatures(21 and 60℃)for various exposition time(15-240 s),on the chemical composition and surface properties of a Fe-13Mn-1.2C biodegradable alloy were *** in mass loss,morphology and wettability evidenced the combined effect of temperature and time for all *** in HCl and HF solutions favor mass loss(0.03-0.1 g/cm^(2))and effectively remove the initial scale.

关键词： Biodegradable metals Iron-based alloys Acid pickling Surface finishing Pre-treatment