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Journal of Integrative Agriculture 2012年第10期11卷 1574-1579页

作者： REN De-yong LI Yun-feng WANG Zeng XU Fang-fang GUO Shuang ZHAO Fang-ming SANG Xian-chun LING Ying-hua HE Guang-hua Key Laboratroy of Application and Safety Control of Genetically Modified crops Rice Research Institute Southwest University

In this study, a rice spikelet mutant, multi-floret spikelet 1 （mfsl）, which was derived from ethylmethane sulfonate （EMS）- treated Jinhui 10 （Oryza sativa L. ssp. indica） exhibited pleiotropic defects in spikelet development. The mfsl spikelet displayed degenerated the empty glume, elongated the rachilla, the extra lemma-like organ and degraded the palea. Additionally, mfsl flowers produced varied numbers of inner floral organs. The genetic analysis revealed that the mutational trait was controlled by a single recessive gene. With 401 recessive individuals from the F2 segregation population, the MFS1 gene was finally mapped on chromosome 5, an approximate 350 kb region. The present study will be useful for cloning and functional analysis of MFS1, which would facilitate understanding of the molecular mechanism involved in spikelet development in rice.

关键词： empty glume gene mapping mfsl mutant rice (Oryza sativa L.) spikelet

A Simple Method for Isolating Chloroplast DNA and Mitochondria DNA from the Same Rapeseed Green Leaf Tissue

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Journal of Integrative Agriculture 2012年第7期11卷 1212-1215页

作者： HU Zhi-yong ZHAN Gao-miao WANG Han-zhong HUA Wei Key Laboratory for Biology and Genetic Improvement of Oil crops Ministry of Agriculture/Oil Crops Research InstituteChinese Academyof Agriculture SciencesWuhan 430062P.R.China

In the study, we present a fast, simple and inexpensive protocol for isolating chloroplast and mitochondrial DNA from one rapeseed leaf tissue sample. The chloroplast and mitochondria were separated from the same green leaf tissue by differential centrifugations. The protocol is the first report that isolates plant chloroplast DNA （cpDNA） and mitochondrial DNA （mtDNA） from the same sample homogenate. The organelle DNA yield is 2-10 micrograms per gram of tissue; the DNA was fully restrictable and was successfully used for sequencing.

关键词： chloroplast DNA mitochondrial DNA organelle isolation rapeseed

Biodiesel Production from Waste Edible Oils and Grease Containing Free Fatty Acids

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China Petroleum Processing & Petrochemical Technology 2005年第3期7卷 33-38页

作者： Huang Fenghong Guo Pingmei Huang Qingde Oil crops Research institute CAAS Wuhan 430062

Till now, most part of the biodiesel is produced from the refined vegetable oils using methanol as feedstock in the presence of an alkali catalyst. However, large amount of waste edible oils and grease are available. The difficulty with alkali-catalyzed esterification of these oils is that they often contain large amount of free fatty acids （FFA）, polymers and decomposition products. These free fatty acids can quickly react with the alkali catalyst to produce soaps that inhibit the separation of the ester and glycerine. An esterification and transesterification process is developed to convert the high FFA oil to its monoesters, The first step, the acidcatalyzed esterification with glycerine and these FFA reduces the FFA content of the oil and grease to less than 3%, and then an azeotropic distillation solvent is used to remove the water. The major factors affecting the conversion efficiency of the process such as glycerol to free fatty acid molar ratio, catalyst amount, reaction temperature and reaction duration are analyzed, The second step, alkali-catalyzed transesterificatiou process converts the products of the first step to its monoesters and glycerol, and then the glycerol is recycled for utilization in the first step. Technical indicators of the biodiesel product can meet the ASTM 6751 standard.

关键词： waste edible grease biodiesel esterification azeotropic distillation solvent glycerol

Metabolomics Analysis of Metabolites in Forsythia suspense Fruit Using UPLC/ESI-Q TRAP-MS/MS

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Phyton-International Journal of Experimental Botany 2022年第10期91卷 2313-2330页

作者： Lingdi Liu Chunxiu Wen Wei Tian Xiaoliang Xie Saiqun Wen Tao Jiang institute of Cash crops Hebei Academy of Agricultural and Forestry SciencesShijiazhuang050051China

Forsythiae Fructus,the fruit of Forsythia suspense is a traditional Chinese hebal medicine that has the antiviral and antioxidant effects in *** analytical chemistry studies showed that the extracts of Forsythiae Fructus contain many bioactive components,such as flavonoids,lignans,phenolic acids,and terpenoids,which can be used to anti-inflammatory and treat toxicity,tonsillitis,ulcers,pharyngitis and acute *** order to study the types and quantities of metabolites in Forsythiae Fructus,we isolated,identified and analysed metabolites between two varieties of Forsythiae Fructus using UPLC/ESI-Q TRAP-MS/*** results showed that a total of 407 metabolites were identified in Forsythiae Fructus using UPLC/ESI-Q TRAP-MS/MS,including 21 terpenoids,68 phenolic acids,63 flavonoids,43 amino acids and derivatives,22 alkaloids,55 lipids,24 lignans and coumarins,31 nucleotides and derivatives,29 organic acids,and 51 other ***,lignans and coumarins,terpenoids,organic acids,lipids,and phenolic acids were rich in Forsythiae Fructus,which accounted for more than 60%of the total metabolite *** metabolite analysis revealed that 80 metabolites differed significantly between the two types of Forsythiae *** results greatly enrich the Forsythiae Fructus phytochemical composition database and provide valuable information for further study of the metabolites of Forsythiae Fructus.

关键词： Forsythiae Fructus traditional Chinese medicine UPLC/ESI-Q TRAP-MS/MS component metabolite

Gene mapping and candidate gene analysis of multi-floret spikelet 3(mfs3) in rice(Oryza sativa L.)

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Journal of Integrative Agriculture 2019年第12期18卷 2673-2681页

作者： ZHENG Hao ZHANG Jun ZHUANG Hui ZENG Xiao-qin TANG Jun WANG Hong-lei CHEN Huan LI Yan LING Ying-hua HE Guang-hua LI Yun-feng Chongqing Key Laboratory of Application and Safety Control of Genetically Modified crops/Rice Research institute Southwest University

Rice(Oryza sativa L.) is one of the most important food crops worldwide and a model monocot plant for gene function analysis, so it is an ideal system for studying flower development. This study reports a mutant, named multi-floret spikelet 3(mfs3), which is related to the spikelet development in rice and derived from the ethylmethane sulfonate(EMS)-treated rice cultivar XIDA 1 B. In mfs3, the main body of palea(bop) was degenerated severely and only glume-like marginal regions of palea(mrp) remained, while other floral organs developed normally, indicating that the palea identity was seriously influenced by the mutation. It was also observed that the number of floral organs was increased in some spikelets, including 2 lemmas, 4 mrp, 4 lodicules, 8–10 stamens, and 2 pistils, which meant that the spikelet determinacy was lost to some degree in mfs3. Furthermore, genetic analysis demonstrated that the mfs3 trait was controlled by a single recessive gene. Using 426 F2 mutants derived from the cross between sterile line 56 S and mfs3, the MULTI-FLORET SPIKELET 3(MFS3) gene was mapped between the molecular markers RM19347 and RM19352 on Chr.6, with a physical distance of 106.3 kb. Sequencing of candidate genes revealed that an 83-bp fragment loss and a base substitution occurred in the LOC_Os06 g04540 gene in the mutant, confirming preliminarily that the LOC_Os06 g04540 gene was the MFS3 candidate gene. Subsequent q PCR analysis showed that the mutation caused the down-regulation of Os MADS1 and FON1 genes, and the up-regulation of Os IDS1 and SNB genes, which are all involved in the regulation of spikelet development. The MFS3 mutation also significantly reduced the transcription of the REP gene, which is involved in palea development. These results indicated that the MFS3 gene might be involved in the spikelet meristem determinacy and palea identity by regulating the expression of these related genes.

关键词： rice(Oryza sativa) multi-floret spikelet palea degeneration gene mappi ng can didate gene

Ethylene response factor BnERF2-like (ERF2.4) from Brassica napus *** submergence tolerance and alleviates oxidative damage caused by submergence in Arabidopsis thaliana

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The Crop Journal 2016年第3期4卷 199-211页

作者： Yanyan Lv Sanxiong Fu Song Chen Wei Zhang Cunkou Qi institute of Industrial crops Jiangsu Academy of Agricultural Sciences Jiangsu Collaborative Innovation Center for Modern Crop Production

Ethylene response factor proteins play an important role in regulating a variety of stress responses in plants,but their exact functions in submergence stress are not well *** this study,we isolated BnE RF2.4 from Brassica napus *** study its function in submergence *** expression of the BnE RF2.4 gene in *** and the expression of antioxidant enzyme genes in transgenic Arabidopsis were analyzed by quantitative *** expression of BnE RF2.4 was induced by submergence in *** and the overexpression of BnE RF2.4 in Arabidopsis increased the level of tolerance to submergence and oxidative stress.A histochemical method detected lower levels of H_2O_2,O^(·-)_2and malondialdehyde(MDA) in transgenic *** to the wild type,transgenic lines also had higher soluble sugar content and higher activity of antioxidant enzymes,which helped to protect plants against the oxidative damage caused by *** was concluded that BnE RF2.4 increased the tolerance of plants to submergence stress and may be involved in regulating soluble sugar content and the antioxidant system in defense against submergence stress.

关键词： Ethylene response factor Submergence Oxidative damage Ectopic expression Arabidopsis Antioxidant enzyme

Analysis of the Influencing Factors of Changes in Foxtail Millet Planting Area in Hebei Province

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Asian Agricultural Research 2013年第3期5卷 30-34页

作者： Meng LIU Yu ZHAO Fei LIU Shunguo LI Guirong WANG Xinshi ZHANG institute of Millet crops Hebei Academy of Agriculture and Forestry Sciences/Cereal Crops Research Laboratory of Hebei Province institute of Agriculture Information and Economics Hebei Academy of Agriculture and Forestry Sciences

Since reform and open to the outside world,foxtail millet planting area in Hebei Province has constantly reduced,and its planting region has changed from the whole province into some regions,with obvious changes. In this paper,by using qualitative and quantitative approaches like chart analysis,summarizing,Delphi method and osculating value model,the influencing factors of changes in millet growing region in Hebei Province were studied. The results showed that the changes of millet planting area were closely related to many factors,such as little investment in scientific research,weak policy support,a single consumer product demand,the rapid development of other staple crops, labor migration,relatively low comparative effectiveness and physiological characteristics of the millet itself. Then some suggestions were put forward,such as increasing research support,strengthening scientific research strength,researching and developing deeply processed products, expanding consumer groups,improving millet policy support,providing subsidies for planting the improved varieties,researching and developing various types of machinery,and increasing millet production technology training.

关键词： Millet area Changes Influencing factors Osculating

Sugar beet yield and industrial sugar contents improved by potassium fertilization under scarce and adequate moisture conditions

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Journal of Integrative Agriculture 2016年第11期15卷 2620-2626页

作者： Muhammad Umair Mubarak Muhammad Zahir Sagheer Ahmad Abdul Wakeel institute of Soil and Environmental Sciences University of Agriculture Faisalabad Sugar crops Program National Agricultural Research Center

Sugar beet（Beta vulgaris L.） is an industrial crop, grown worldwide for sugar production. In Pakistan, sugar is mostly extracted from sugarcane, soil and environmental conditions are equally favorable for sugar beet cultivation. Beet sugar contents are higher than sugarcane sugar contents, which can be further increased by potassium（K） fertilization. Total K concentration is higher in Pakistani soils developed from mica minerals, but it does not represent plant available K for sustainable plant growth. A pot experiment was conducted in the wire-house of institute of Soil and Environmental Sciences at University of Agriculture Faisalabad, Pakistan. K treatments were the following： no K（K_0）, K application at 148 kg ha^（–1）（K_1） and 296 kg ha^（–1）（K_2）. Irrigation levels were used as water sufficient at 60% water holding capacity and water deficient at 40% water holding capacity. The growth, yield and beet quality data were analyzed statistically using LSD. The results revealed that increase in the level of K fertilization at water sufficient level significantly increased plant growth, beet yield and industrial beet sugar content. The response of K fertilization under water deficient condition was also similar, however overall sugar production was less than that in water sufficient conditions. It is concluded from this study that K application could be used not only to enhance plant growth and beet yield but also enhance beet sugar content both under water-deficient as well as water-sufficient conditions.

关键词： potassium irrigation levels beet yields sugar contents

A novel long non-coding RNA, DIR, increases drought tolerance in cassava by modifying stress-related gene expression

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Journal of Integrative Agriculture 2022年第9期21卷 2588-2602页

作者： DONG Shi-man XIAO Liang LI Zhi-bo SHEN Jie YAN Hua-bing LI Shu-xia LIAO Wen-bin PENG Ming Key Laboratory of Biology and Genetic Resources of Tropical crops Institute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agricultural SciencesHaikou 571101P.R.China Key Laboratory for Biology and Genetic Resources of Tropical crops of Hainan Province Hainan Institute for Tropical Agricultural ResourcesHaikou 571101P.R.China Cash crops Research institute Guangxi Academy of Agricultural SciencesNanning 530007P.R.China College of Tropical crops Hainan UniversityHaikou 570228P.R.China

Cassava is an important tropical cash crop. Severe drought stresses affect cassava productivity and quality, and cause great economic losses in agricultural production. Enhancing the drought tolerance of cassava can effectively improve its yield. Long non-coding RNAs(lncRNAs) are present in a wide variety of eukaryotes. Recently, increasing evidence has shown that lncRNAs play a critical role in the responses to abiotic stresses. However, the function of cassava lncRNAs in the drought response remains largely unknown. In this study, we identified a novel lncRNA, DROUGHT-INDUCED INTERGENIC lncRNA(DIR). Gene expression analysis showed that DIR was significantly induced by drought stress treatment, but did not respond to abscisic acid(ABA) or jasmonic acid(JA) treatments. In addition, overexpression of the DIR gene enhanced proline accumulation and drought tolerance in transgenic cassava. RNA-seq analysis revealed that DIR preferentially affected drought-related genes that were linked to transcription and metabolism. Moreover, RNA pull-down mass spectrometry analysis showed that DIR interacted with 325 proteins. A protein–protein interaction(PPI)analysis found a marked enrichment in proteins associated with the mRNA export and protein quality control ***, these results suggest that DIR and its interacting proteins that regulate mRNA or protein metabolism are involved in mediating the drought stress response. Thus, regulating DIR expression has potential for improving cassava yield under drought conditions.

关键词： lncRNA RNA-seq drought stress cassava DIR

Comparative transcriptome analysis of sweet corn seedlings under low-temperature stress

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The Crop Journal 2017年第5期5卷 396-406页

作者： Jihua Mao Yongtao Yu Jing Yang Gaoke Li Chunyan Li Xitao Qi Tianxiang Wen Jianguang Hu Guangdong Provincial Key Laboratory of crops Genetics and Improvement Crop Research Institute Guangdong Academy of Agricultural Sciences

Stress induced by low temperature, which represents a widespread environmental factor,strongly affects maize growth and yield. However, the physiological characteristics and molecular regulatory mechanisms of maize seedlings in response to cold remain poorly understood. In this study, using RNA-seq, we investigated the transcriptome profiles of two sweet com inbred lines, "Richao"(RC) and C5, under cold stress. A total of 357 and 455 differentially expressed genes(DEGs) were identified in the RC and C5 lines, respectively, 94 DEGs were detected as common DEGs related to cold response in both genotypes, and a total of 589 DEGs were detected as cold tolerance-associated genes. By combining protein function clustering analysis and significantly enriched Gene Ontology(GO) terms analysis,we suggest that transcription factors may play a dominating role in the cold stress response and tolerance of sweet com. Furthermore, 74 differentially expressed transcription factors were identified, of those many genes involved in the metabolism and regulation of hormones. These results expand our understanding of the complex mechanisms involved in chilling tolerance in maize, and provide a set of candidate genes for further genetic analyses.

关键词： Maize (Zea mays L.) Low temperature stress RNA-seq Cold-responsive gene