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Analysis of an Applied Core Collection of Adzuki Bean Germplasm by Using SSR Markers

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Journal of Integrative Agriculture 2012年第10期11卷 1601-1609页

作者： WANG Li-xia CHENG Xu-zhen WANG Su-hua TIAN Jing institute of Crop Sciences Chinese Academy of Agricultural Sciences institute of cereal and Oil crops Hebei Academy of Agricutural and Forestry Sciences

Genetic diversity of 158 accessions of an applied core collection of adzuki bean （Vigna angularis） and 18 wild genotypes were assessed by using 85 microsatellite markers. With an average of 5.81 alleles per locus, 493 alleles were detected, and their distribution frequencies lower than 5% accounted for 73.02% of the total number. The distributions of alleles between the cultivated and the wild adzuki bean germplasm are different, with a higher allelic diversity in the wild germplasm than that of the cultivated ones. An obvious genetic differentiation was also observed between the wild and the cultivated adzuki beans, and SSR markers may be useful in study identification and classification of them. Among cultivated adzuki bean, the genetic similarity coefficient varied from 0.366 to 0.939. Genetic structure analysis can clearly separate the wild genotypes from the cultivated adzuki bean, and also can divide the cultivated ones into different populations, as these populations are closely agreeable with the ecological regions where they originally grow. The results of this study will be useful in arranging local breeding programs, especially in the aspect of parental combinations or identification of progenies. These SSR markers can also provide important information to explain the genetic relationship between the cultivated and wild adzuki beans, and to accelerate the wild gene resources in broadening the gene pool in breeding program.

关键词： adzuki bean applied core collection genetic variations SSR marker wild genotype

Genomic and transcriptomic insights into cytochrome P450 monooxygenase genes involved in nicosulfuron tolerance in maize(Zea mays L.)

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Journal of Integrative Agriculture 2018年第8期17卷 1790-1799页

作者： LIU Xiao-min XU Xian LI Bing-hua YAO Xiao-xia ZHANG Huan-huan WANG Gui-qi HAN Yu-jun institute of cereal and Oil crops Hebei Academy of Agriculture and Forestry Sciences Xingtai Academy of Agriculture Sciences College of Agriculture Northeast Agricultural University

Postemergence application of nicosulfuron for weed control in maize fields can cause great damage to certain maize inbred lines and hybrids. Two maize genotypes, tolerant inbred(HBR) and sensitive inbred(HBS), were found to significantly differ in their phenotypic responses to nicosulfuron, with the EC50(50% effective concentration) values differed statistically(763.6 and 5.9 g a.i. ha–1, respectively). Pre-treatment with malathion, a known cytochrome P450 inhibitor, increased nicosulfuron injury in both HBR and HBS. Our results support the hypothesis that nicosulfuron selectivity in maize is associated with cytochrome P450 metabolism. Further analysis of the maize genome resulted in the identification of 314 full length cytochrome P450 monooxygenase(CYP) genes. These genes were classified into 2 types, 10 clans and 44 families. The CYP71 clan was represented by all A-type genes(168) belonging to 17 families. Nine clans possessed 27 families containing 146 non-A-type genes. The consensus sequences of the heme-binding regions of A-type and non-A-type CYP proteins are ‘PFGXGRRXCPG’ and ‘FXXGPRXCXG’, respectively. Illumina transcriptome sequence results showed that there were 53 differentially expressed CYP genes on the basis of high variation in expression between HBS and HBR, nicosulfuron-treated and untreated samples. These genes may contribute to nicosulfuron tolerance in maize. A hierarchical clustering analysis obtained four main clusters named C1 to C4 in which 4, 15, 21, and 13 CYP genes were found in each respective cluster. The expression patterns of some CYP genes were confirmed by RT-q PCR analysis. The research will improve our understanding of the function of maize cytochrome P450 in herbicide metabolism.

关键词： cytochrome P450 Zea mays L. malathion nicosulfuron herbicide metabolism

Syntenic quantitative trait loci and genomic divergence for Sclerotinia resistance and ?owering time in Brassica napus

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Journal of Integrative Plant Biology 2019年第1期61卷 75-88页

作者： Fengqi Zhang Junyan Huang Minqiang Tang Xiaohui Cheng Yueying Liu Chaobo Tong Jingyin Yu Tehrim Sadia Caihua Dong Lingyan Liu Baojun Tang Jianguo Chen Shengyi Liu Key Laboratory of Biology and Genetic Improvement of Oil crops the Ministry of AgricultureOil Crops Research Institute of CAAS cereal crops Research institute Henan Academy of Agricultural Sciences Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources Faculty of Life ScienceHubei University

Oilseed rape(Brassica napus) is an allotetraploid with two subgenomes descended from a common ancestor. Accordingly, its genome contains syntenic regions with many duplicate genes, some of which may have retained their original functions, whereas others may have diverged. Here, we mapped quantitative trait loci(QTL) for stem rot resistance(SRR), a disease caused by the fungus Sclerotinia sclerotiorum, and flowering time(FT) in a recombinant inbred line population. The population was genotyped using *** 60 K single nucleotide polymorphism arrays and phenotyped in six(FT) and nine(SSR) experimental conditions or environments. In total, we detected 30 SRR QTL and 22 FT QTL and show that some of the major QTL associated with these two traits were co-localized,suggesting a genetic linkage between them. Two SRR QTL on chromosome A2 and two on chromosome C2 were shown to be syntenic, suggesting the functional conservation of these regions. We used the syntenic properties of the genomic regions to exclude genes for selection candidates responsible for QTL-associated traits. For example, 152 of the 185 genes could be excluded from a syntenic A2–C2 region. These findings will help to elucidate polyploid genomics in future studies, in addition to providing useful information for B. napus breeding programs.

关键词： napus Oilseed rape

Fine mapping of a major QTL qHYF_B06 for peanut yield

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The Crop Journal 2023年第5期11卷 1533-1540页

作者： Yongqing Yang Qiao Su Yurong Li Zengshu Cheng Yahui Song Xinxin Jin Jin Wang institute of cereal and Oil crops Hebei Academy of Agricultural and Forestry Sciencesthe Key Laboratory of Crop Genetics and Breeding of HebeiShijiazhuang 050035HebeiChina

High yield is a major objective for peanut(Arachis hypogaea L.) breeding worldwide. However, fewer yield-related quantitative trait loci(QTL) have been reported in peanut than in other staple food crops such as rice(Oryza sativa), wheat(Triticum aestivum), and maize(Zea mays). This study aimed to identify stable major-effect QTL associated with pod yield per plant, hundred-pod weight for double-seeded pods,hundred-seed weight, shelling percentage, and pod number per plant, allowing us to predict candidate genes by means of transcriptome and genome sequencing. To this end, we used a population of recombinant inbred lines comprising 192 F9:11families derived from a JH6 × KX01-6 cross to construct a highresolution genetic map(1705.7 c M) consisting of 2273 polymorphic SNPs, with 0.75 c M(on average)between adjacent SNPs. We identified two high-confidence, yield-related QTL, qHYF_A08 and qHYF_B06, explaining 5.78%–31.40% of phenotypic variation and with LOD values of 5.10–24.48, in six environments. qHYF_A08 mainly explained the variation in shelling percentage, whereas qHYF_B06explained variation in hundred-pod weight and hundred-seed weight and accounted for 8.77%–31.40%of the variation in effective pod number per plant, pod number per plant, and shelling percentage. We narrowed down qHYF_B06 to an 890-kb interval using an advanced mapping *** and genome analyses revealed that only Arahy.129FS0 and Arahy.3R9A5K in the candidate mapping interval were differentially expressed between JH6 and KX01-6, with substantial structural variations in their promoter and coding regions. Genotypes of 208 peanut accessions determined using a diagnostic CAPS marker suggested that the two haplotypes of Arahy.3R9A5K were highly associated with hundred-seed weight and hundred-pod weight;this diagnostic CAPs marker could therefore be useful for selecting high-yielding lines during peanut breeding. Overall, our results provide valuable information for cloning alleles w

关键词： Arachis hypogaea Candidate gene E3 ubiquitin ligase Yield-related QTL region

Isolation and Analysis of Drought-Induced Genes in Maize Roots

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Agricultural Sciences in China 2009年第2期8卷 129-136页

作者： LI Hui-yong HUANG Shu-hua SHI Yun-su SONG Yan-chun ZHONG Zhong-bao WANG Guo-ying WANG Tian-yu LI Yu institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China The cereal crops institute Henan Academy of Agricultural Sciences Zhengzhou 450002 P.R.China

Maize roots are important component for plant adaptation to soil water deficits because they are supposed to take up water and necessary solutes from the soil. In the present study, the drought-induced genes were isolated in maize roots. A suppression subtractive hybridization protocol was applied to construct a forward subtractive cDNA library from CN165 for drought-stressed maize roots and a number of drought-induced genes were isolated. Totally, 126 uniESTs （containing 82 singlets and 44 contigs） were obtained from 503 available ESTs sequences after macroarray hybridization. UniESTs were analyzed using BLASTN and BLASTX and the results showed that 92% of the uniESTs had homolgous sequences in maize nr database by BLASTN. About 89% of uniESTs appeared the homlogous amino acid sequences in rice protein database but not in maize protein database by BLASTX, implying that those genes are likely new functional genes in maize. Function analysis showed that those genes were involved in a broad spectrum of biological pathways, mainly in signaling and regulatory pathways related to stress tolerance.

关键词： maize roots drought stress suppression subtractive hybridization expressed sequence tag

Wheat Elongator subunit 4 is required for epigenetic regulation of host immune response to Rhizoctonia cerealis

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The Crop Journal 2020年第4期8卷 565-576页

作者： Kai Wang Wei Rong Yuping Liu Hui Li Zengyan Zhang The National Key Facility for Crop Gene Resources and Genetic Improvement Key Laboratory of Biology and Genetic Improvement of Triticeae CropsMinistry of AgricultureInstitute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China institute of cereal and Oil crops Hebei Academy of Agriculture and Forestry SciencesShijiazhuang 050035HebeiChina

Wheat(Triticum aestivum)is necessary for global food *** necrotrophic fungus Rhizoctonia cerealis is the causal agent of sharp eyespot,a devastating disease of *** the Elongator complex,composed of six subunits,has been implicated in growth,development,and innate immunity in Arabidopsis,little is known about its functions in wheat or the involvement of Elongator subunit 4 in histone *** this study,we identified the Elongator subunit 4-encoding gene Ta ELP4 in wheat resistance response to ***,and verified that Ta ELP4 increased histone acetylation in regions of defenseassociated genes and regulated immune response to *** ELP4 was more highly expressed in resistant than in susceptible wheat cultivars and was induced in resistant wheat after infection by *** of Ta ELP4 in wheat not only impaired resistance to ***,but also repressed both histone acetylation levels and the expression of a subset of defense-associated genes,including Ta AGC1,Ta CPK7-D,Ta PAL5,Defensin,and *** expression of Ta ELP4 in Arabidopsis increased histone acetylation levels in coding and promoter regions of defense genes and increased their transcription,leading to increased resistance to infection by the pathogen Botrytis *** results suggest that Ta ELP4 positively regulates innate immune responses of wheat and Arabidopsis to *** and *** by increasing histone acetylation levels of defense-associated genes and increasing their *** study has shed light on the involvement of Ta ELP4 in histone acetylation and resistance response against *** ELP4 may potentially be used to improve wheat resistance against sharp eyespot.

关键词： Wheat resistance Arabidopsis

A systematic review of highland barley:Ingredients,health functions and applications

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Grain & Oil Science and Technology 2022年第1期5卷 35-43页

作者： Yiming Lyu Sen Ma Jingke Liu Xiaoxi Wang College of Food Science and Engineering Henan University of TechnologyZhengzhou 450001China institute of Millet crops Hebei Academy of Agriculture and Forestry SciencesNational FoxtailMillet Improvement CenterMinor Cereal Crops Laboratory of Hebei ProvinceShijiazhuang 050035China

Due to its high nutritional and dietotherapy values,highland barley has attracted the attention and favor of people all over the world in recent *** has been demonstrated that the nutritional components of highland barley are comprehensive and unique,with the characteristics of high protein,high fiber,high vitamin,lowfat,low sugar and variety of bioactive *** the most important component in highland barley,highland barley starch not only has low digestibility,but also has good freeze-thaw stability,high solubility,good emulsion stability and superior film forming performance,which makes it have great application value in food,medicine and industrial *** content of highland barley protein,which is rich in 18 kinds of amino acids,is higher than that of most grains,and its derivatives play an important role in medical ***,highland barley protein cannot form gluten network structure,which limits the application in daily staple food to a certain *** barley also contains a large amount of dietary fiber,especiallyβ-***-term consumption could significantly reduce the incidence of chronic diseases and metabolic syndromes such as heart disease and ***,the research on the application of highland barley is still in the laboratory stage,which failed to achieve large-scale application in the actual *** value of highland barley has not been brought into full play,which leads to the waste of its resources and the reduction of its added *** paper reviewed themacronutrients,health functions and applications of highland barley,aiming to provide some reference for the development of highland barley in food and health industry.

关键词： Highland barley Macronutrients Physicochemical property Application

Mapping QTL for flowering time-related traits under three plant densities in maize

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The Crop Journal 2021年第2期9卷 372-379页

作者： Liwei Wang Zhiqiang Zhou Ronggai Li Jianfeng Weng Quanguo Zhang Xinghua Li Baoqiang Wang Wenying Zhang Wei Song Xinhai Li The Key Laboratory of Crop Genetics and Breeding of Hebei Province Institute of Cereal and Oil CropsHebei Academy of Agriculture and Forestry SciencesShijiazhuang 050035HebeiChina institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081China

Flowering time is an indicator of adaptation in maize and a key trait for selection in *** genetic basis of flowering time in maize,especially in response to plant density,remains *** objective of this study was to identify maize quantitative trait loci(QTL)associated with flowering time-related traits that are stably expressed under several plant densities and show additive effects that vary with plant *** hundred recombinant inbred lines(RIL)derived from a cross between Ye 478 and Qi 319,together with their parents,were planted at three plant densities(90,000,120,000,and 150,000 plants ha^(-1))in four *** five traits investigated were days to tasseling(DTT),days to silking(DTS),days to pollen shed(DTP),interval between anthesis and silking(ASI),and interval between tasseling and anthesis(TAI).A high-resolution bin map was used for QTL *** the RIL population,the DTT,DTS,and DTP values increased with plant density,whereas the ASI and TAI values showed negligible response to plant density.A total of 72 QTL were identified for flowering time-related traits,including 15 stably expressed across *** flowering time under different densities seems to be regulated by complex pathways rather than by several major genes or an independent *** effects of some stable QTL,especially qDTT8-1 and qDTT10-4,varied with plant *** mapping and cloning of these QTL will shed light on the mechanism of flowering time and assist in breeding earlymaturing maize inbred lines and hybrids.

关键词： Maize Flowering time Plant density Recombinant inbred lines(RIL) Genetic basis

QTL Mapping of Isoflavone,Oil and Protein Contents in Soybean (Glycine max ***.)

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Agricultural Sciences in China 2010年第8期9卷 1108-1116页

作者： LIANG Hui-zhen YU Yong-liang WANG Shu-feng LIAN Yun WANG Ting-feng WEI Yan-li GONG Peng-tao LIU Xue-yi FANG Xuan-jun ZHANG Meng-chen Industrial crops Research institute Henan Academy of Agricultural Sciences/National Soybean Improvement Sub-Center of Zhengzhou Zhengzhou 450002 P.R.China Hainan institute of Tropieal Agriculture Resources Sanya 572025 P.R. China Economical crops institute Shanxi Academy of Agricultural Sciences Fenyang 032200 P.R.China Institue of cereal and Oil crops Hebei Academy of Agricultural and Forestry Sciences Shijiazhuang 050031 P.R. China

Soybean （Glycine max L. Merr.） is the world＇s foremost source of edible plant oil and proteins, meantime, the biologically active secondary metabolites such as saponins and isoflavones are benefit to human health. The objective of this study was to identify quantitative trait loci （QTL） and epistatic interactions associated with isoflavone, protein, and oil contents in soybean seeds. An F13 recombinant inbred line （RIL） comprising 474 lines was derived from a cross between Jindou 23 and Huibuzhi cultivars. SSR technique was employed for mapping of the QTLs. The QTLs for isoflavone, protein, and oil contents were analyzed and 23 QTLs were detected based on the constructed linkage map. Six QTLs for isoflavone content were localized in linkage groups J, N, D2, and G, eleven QTLs for oil content were localized in the linkage groups A1, A2, B2, C2, and D2, and six QTLs for protein content were localized in linkage groups B2, C2, G, and H1. The correlative analysis demonstrated that the isoflavone content had significant correlation with protein content, while significantly negative correlations was existed between oil and protein content, and significantly positive correlations was existed between protein and oil content. All these findings have laid an important basis for the marker assisted breeding in soybean. The phenotypic correlations of quantitative traits may be resulted from the correlation of the QTL controlling those traits.

关键词： soybean SSR QTL quality isoflavone