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Neural Regeneration Research 2025年第3期20卷 805-807页

作者： JoséM.Izquierdo centro de Biología Molecular Severo Ochoa(CBM) Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid(CSIC-UAM)MadridSpain

The word “senescence” comes from the Latin senescens, meaning “to begin to age”, and is characterized by a long-lasting but reversible block in proliferation, resulting from stress-induced cell cycle arrest of pre... 详细信息

关键词： Latin potential lasting

Reassessing the AMPK-MTORC1 balance in autophagy in the central nervous system

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Neural Regeneration Research 2025年第11期20卷 3209-3210页

作者： Marta García-Juan Mario Villa Irene Benito-Cuesta Lara Ordónez-Gutiérrez Francisco Wandosell centro de Biología Molecular“Severo Ochoa”(csic-UAM) Nicolas Cabrera 1Universidad Autónoma de MadridMadridSpain Fisiología Animal Departamento de BiologíaFacultad de CienciasUniversidad Autónoma de MadridMadridSpain department of Clinical Neuroscience CMM Karolinska Universitetssjukhuset SolnaStockholmSweden departamento de Bioquímica y Biología Molecular Universidad Complutense de MadridMadridSpain centro de Investigación Biomédica en Red de Enfermedades Neurodegenerativas(CIBERNED) MadridSpain

Autophagy is a cellular degradation and recycling system,indispensable for cellular and organ development,homeostasis,and *** cellular process is evolutionarily highly conserved to quality control of many proteins and dysfunctional organelles,which finally recycle components as amino *** process is effective during normal physiology as part of anabolism and plays an additional important role during starvation(Dikic and Elazar,2018).Different types of autophagy have been chara cterized based on their dynamic,mechanism of action,target substrates,and protein *** of them are macroautophagy(hereafter called"autophagy"),microa utophagy,and chape rone-media ted autophagy(Fleming et al.,2022).

关键词： homeostasis system hereafter

Prefoldins 3 and 5 Play an Essential Role in Arabidopsis Tolerance to Salt Stress

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Molecular Plant 2009年第3期2卷 526-534页

作者： Miguel A. Rodriguez-Milla Julio Salinas departamento de Biologfa de Plantas Centro de Investigaciones Biologicas-CSIC Ramiro de Maeztu 9 28040 Madrid Spain

During the last years, our understanding of the mechanisms that control plant response to salt stress has been steadily progressing. Pharmacological studies have allowed the suggestion that the cytoskeleton may be involved in regulating such a response. Nevertheless, genetic evidence establishing that the cytoskeleton has a role in plant tolerance to salt stress has not been reported yet. Here, we have characterized Arabidopsis T-DNA mutants for genes encoding proteins orthologous to prefoldin （PFD） subunits 3 and 5 from yeast and mammals. In these organisms, PFD subunits, also known as Genes Involved in Microtubule biogenesis （GIM）, form a heterohexameric PFD complex implicated in tubulin and actin folding. We show that, indeed, PFD3 and PFD5 can substitute for the loss of their yeast orthologs, as they are able to complement yeast gim2Δ and gim5Δ mutants, respectively. Our results indicate that pfd3 and pfd5 mutants have reduced levels of α- and β-tubulin compared to the wild-type plants when growing under both control and salt-stress conditions. In addition, pfd3 and pfd5 mutants display alterations in their developmental patterns and microtubule organization, and, more importantly, are hypersensitive to high concentrations of NaCl but not of LiCl or mannitol. These results demonstrate that the cytoskeleton plays an essential role in plant tolerance to salt stress.

关键词： Prefoldins cytoskeleton tubulin actin microtubule salt tolerance Arabidopsis

Insights of the brain damage response using antibodies identifying surface antigens on neural stem cells and neuroblasts

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Neural Regeneration Research 2015年第10期10卷 1574-1575页

作者： Silvia Santamaria Jose A.Garcia-Sanz department of Cellular and Molecular Medicine Centro de Investigaciones Biologicas (CIB-CSIC)

Similarly to other adult tissues,a hierarchical structure has been established for the brain,where the differentiated cell types（neurons,oligodendrocytes and astrocytes）are generated from primary progenitor cells,known as type B astrocytes or neural stem cells （NSC）, through one or multiple stages of amplification （transient amplifying cells） that generate precursor cells （iPC） with more restricted potential nlPCs （neural）, alPCs （astrocytes）, olPC （oligodendrocytes）（Kriegstein and Alvarez-Buvlla, 2009）.

关键词： NSCs stem

Farm-scale evaluation of the impact of CrylAb Bt maize on canopy nontarget arthropods： a 3-year study

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Insect Science 2018年第1期25卷 87-98页

作者： Maria Arias-Martin Matias Garcia Pedro Castanera Felix Ortego Gema P. Farinos centro de investigaciones Biologicas CSIC Departamento de Biologia Medioambiental Grupo de Interaccion Planta-lnsecto Ramiro deMaeztu 9 28040 Madrid Spain

The cultivation of CrylAb-expressing genetically modified MON810 （Bt maize） has led to public concern in Europe, regarding its impact on nontarget arthro- pods （NTAs）. We have assessed the potential effects of DKC 6451 YG （MON810） maize on canopy NTAs in a farm-scale study performed in Central Spain during 3 years. The study focused on hemipteran herbivores （leafhoppers and planthoppers） and hymenopteran parasitic wasps （mymarids） collected by yellow sticky traps, which accounted for 72% of the total number of insects studied. The dynamics and abundance of these groups varied among years, but no significant differences were found between Bt and non-Bt maize, indicating that Bt maize had no negative effect on these taxa. Nonetheless, the CrylAb toxin was detected in 2 different arthropods collected from Bt maize foliage, the cicadellids Zyginidia scutellaris and Empoasca spp. A retrospective power analysis on the arthropod abundance data for our field trials has determined that Z. scutellaris and the family My- maridae have high capacity to detect differences between the Bt maize and its isogenic counterpart. The use of these canopy NTAs as surrogates for assessing environmental impacts of Bt maize is discussed.

关键词： Cicadellidae MON810 Mymaridae risk assessment surrogate species Zyginidia scutellaris

CBFs at the Crossroads of Plant Hormone Signaling in Cold Stress Response

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Molecular Plant 2017年第4期10卷 542-544页

作者： Javier Barrero-Gil Julio Salinas departamento de Biología Medioambiental Centro de Investigaciones Biológicas-CSIC Ramiro de Maeztu 9 28040 Madrid Spain

Brassinosteroids （BRs） are steroid plant hormones with important roles in plant growth and development through the regulation of processes such as cell division and expansion. In addition to their role as growth promoters, BRs can also protect plants from a va- riety of abiotic stresses, including drought, salt, heat, and cold stress. Nevertheless, the molecular determinants underlying this protective effect remain largely uncharacterized.

关键词： CBFs Crossroads Plant Hormone Signaling

Identification of SUMO Targets by a Novel Proteomic Approach in Plants

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Journal of Integrative Plant Biology 2013年第1期55卷 96-107页

作者： Gema López-Torrejón Davide Guerra Rafael Catal Julio Salinas Juan C. del Pozo centro de Biotecnología y Genómica de Plantas (INIA-UPM) Campus de Montegancedo CRA-Genomics Research Centre (GPG) centro de investigaciones Biológicas (csic) Campus de la Ciudad Universitaria de Madrid

Post-translational modifications （PTMs） chemically and physically alter the properties of proteins, including their folding, subcellular localization, stability, activity, and consequently their function. In spite of their relevance, studies on PTMs in plants are still limited. Small Ubiquitin-like Modifier （SUMO） modification regulates several biological processes by affecting protein-protein interactions, or changing the subcellular Iocalizations of the target proteins. Here, we describe a novel proteomic approach to identify SUMO targets that combines 2-D liquid chromatography, immunodetection, and mass spectrometry （MS） analyses. We have applied this approach to identify nuclear SUMO targets in response to heat shock. Using a bacterial SUMOylation system, we validated that some of the targets identified here are, in fact, labeled with SUMO1. Interestingly, we found that GIGANTEA （GI）, a photoperiodic-pathway protein, is modified with SUMO in response to heat shock both in vitro and in vivo.

关键词： Mass spectrometry plants post-translational modification proteomics SUMO.

A role for the Tgf-β/Bmp co-receptor Endoglin in the molecular oscillator that regulates the hair follicle cycle

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Journal of Molecular Cell Biology 2019年第1期11卷 39-52页

作者： Maria I.Calvo-Sanchez Sandra Fernandez-Martos Elisa Carrasco Gema Moreno-Bueno Carmelo Bernabeu Miguel Quintanilla Jesus Espada Instituto de investigaciones Biomedicas'Alberto Sols' Consejo Superior de Investigaciones Cientificas(CSIC)-Departamento de BioqufmicaUniversidad Autonoma de Madrid(UAM)Madrid 28029Spain Instituto de investigaciones Biosanitarias Facultad de Ciencias ExperimentalesUniversidad Francisco de Vitoria(UFV)Pozuelo de Alarcon 28223Spain centro de Investigacion Biomedica en Red de Cancer(CIBERONC) Spain centro de investigaciones Biologicas Consejo Superior de Investigaciones Cientificas(CSIC)and Centro de Investigacion Biomedica en Red de Enfermedades Raras(CIBERER)Madrid 28040Spain centro Integrativo de Biologfa y Quimica Aplicada Universidad Bernardo O'HigginsSantiago 8370854Chile Instituto Ramon y Cajal de Investigacion Sanitaria(IRYCIS) Hospital Universitario Ramon y CajalMadridSpain

The hair follicle is a biological oscillator that alternates growth,regression,and rest phases driven by the sequential activation of the proliferation differentiation programs of resident stem cell *** activation of hair follicle stem cell niches and subsequent entry into the growing phase is mainly regulated by Wnt/β-catenin signalling,while regression and resting phases are mainly regulated by Tgf-β/Bmp/Smad activity.A major question still unresolved is the nature of the molecular switch that dictates the coordinated transition between both signalling *** we have focused on the role of Endoglin(Eng),a key co-receptor for members of the Tgf-β/Bmp family of growth *** an Eng haploinsufficient mouse model,we report that Eng is required to maintain a correct follicle cycling pattern and for an adequate stimulation of hair follicle stem cell *** further report thatβ-catenin binds to the Eng promoter depending on Bmp ***,we show thatβ-catenin interacts with Smad4 in a Bmp/Eng-dependent context and both proteins act synergistically to activate Eng promoter *** observations point to the existence of a growth/rest switching mechanism in the hair follicle that is based on an Eng-dependent feedback cross-talk between Wnt/β-catenin and Bmp/Smad signals.

关键词： Endoglin hair follicle skin stem cells Wnt/β-catenin Tgf-β/Bmp/Smad

Response of Soil Microbial Community to a High Dose of Fresh Olive Mill Wastewater

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Pedosphere 2013年第3期23卷 281-289页

作者： J.L.MORENO F.BASTIDA M.A.S NCHEZ-MONEdeRO T.HERNNdeZ C.GARCíA department of Soil and Water Conservation Centro de Edafología y Biología Aplicada del Segura-Consejo Superior de Investigaciones Científicas (CEBAS-CSIC) Campus Universitario de Espinardo

An incubation experiment was designed in order to determine the further microbiological response to an addition （500 m3 ha-1） of fresh olive mill wastewater （FOMWW） in a soil that has been frequently amended with uncontrolled doses of OMWW since the 1990s in an active disposal site （ADS soil）. To achieve this aim, the phospholipid fatty acid （PLFA） profiles, microbial biomass C （Cmic）, and dehydrogenase （DHA） and urease activities （URA） were monitored at the beginning （To）, 3 h （T1） and 97 d （Tf, i.e., the end） of incubation after FOMWW addition. After the FOMWW addition, an increase in the ratio of fungal to bacterial PLFAs was observed in ADS soil. Moreover, a relative increase of monounsaturated fatty acids （MUFAs） with respect to saturated fatty acids （SATFA） was found in the ADS soil. An increase of the Gram-positive to Gram-negative ratio was observed in this soil at the end of the incubation. While DHA and Cmic increased in the ADS soil after FOMWW addition, URA showed a decrease. Fungi and Gram-positive bacterial biomass experienced an increase after addition of a high dose of FOMWW in laboratory conditions.

关键词： enzyme activity microbial activity microbial biomass phospholipid fatty acid (PLFA) biomarker

A Single JAZ Repressor Controls the Jasmonate Pathway in Marchantia polymorpha

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Molecular Plant 2019年第2期12卷 185-198页

作者： Isabel Monte Jose M. Franco-Zorrilla Gloria Garcia-Casado Angel M. Zamarreno Jose M. Garcia-Mina Ryuichi Nishihama Takayuki Kohchi Roberto Solano department of Plant Molecular Genetics Centro Nacional de Biotecnologia Consejo Superior de Investigaciones Cientificas (CNB-CSIC) 28049 Madrid Spain Genomics Unit Centro Nacional de Biotecnologia Consejo Superior de Investigaciones Cientificas (CNB-CSIC) 28049 Madrid Spain Environmental Biology department University of Navarra Navarre Spain Graduate School of Biostudies Kyoto University Kyoto 606-8502 Japan

JAZ proteins are negative regulators of jasm onate responses,acting both as repressors of transcription factors and as co-receptors of JA-lle. The high redundancy of JAZ genes in angiosperms has hindered the characterization of a complete depletion of JAZ function. Moreover, the recent discovery that dn- OPDA is the jasmonate ligand in Marchantia polymorpha demonstrates that JA-lle is not the sole COI1/ JAZ ligand in land plants and highlights the importance of studying JAZ co-receptors in bryophytes? Here, we have exploited the low gene redundancy of the liverwort M. polymorpha to characterize the single MpJ4Z in this early diverging plant lineage. We clarify the phylogenetic history of the TIFY family, demonstrate that MpJAZ is the ortholog of AtJAZ with a conserved function, and characterize its repressor activity of dn-OPDA resp on ses. Our results show that, con sistent with previous findings in Arabidopsis, MpJAZ represses jasmonates biosynthesis, senescence, and plant defenses, and promotes cell growth and reproductive fitness, highlighting the power of studies in Marchantia.

关键词： Jasmonate repressors Marchantia Hormone signaling evolution gene redundancy