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Identification and Expression of Some Plant cell Wall-degrading enzymes Present in Three Ontogenetics Stages of Thecaphora frezii, a Peanut (<i>Arachis hypogaea</i>L.) Pathogenic Fungus

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American Journal of Plant Sciences 2022年第1期13卷 1-22页

作者： Néstor Walter Soria Ana cristina Figueroa María Soledad Díaz Valeria Roxana Alasino Pablo yang Dante Miguel Beltramo cátedra de Biotecnología Facultad de Ciencias Químicas Unidad Asociada al CONICET: área de Cs. Agrarias Ingeniería Cs. Biológicas Universidad Católica de Córdoba Córdoba Argentina centro de excelencia en productos y procesos de córdoba—ceprocor Santa María de Punilla Córdoba Argentina cONIcET Santa María de Punilla Córdoba Argentina

Peanuts can be affected by the presence of pathogenic microorganisms. The fungus Thecaphora frezii (T. frezii), which belongs to the taxonomic class Ustilaginomycetes, is the causal agent of the disease known as “peanut smut”. The life cycle of this fungus includes three stages, namely teliospores, basidiospores and hyphae. In the hyphae stage, infection occurs in the peanut plant, which requires the involvement of some enzymes secreted by the fungus. These include the Plant cell Wall-degrading enzymes (PcWdes), which degrade various polysaccharides. This study aimed to identify the presence of transcript for enzymes belonging to the PcWdes from three stages of T. frezii. For this, total RNA was extracted from the three ontogenetic stages of T. frezii. These samples were analyzed using an RNA-Seq approach and some transcripts were quantified using Real Time PcR. The analysis of the data provided by the RNA-Seq of the three T. frezii stages, it was possible to identify some transcripts that could encode enzymes compatible with polysaccharides degradation that are part of the plant cell wall. In T. frezii transcriptome, 40 deduced proteins would be enzymes with functions of PcWdes were identified. They were divided into 27 glycoside hydrolases;two polysaccharide lyases;three carbohydrate esterases and eight enzymes with auxiliary activities. In addition, the fungal SNF1 gene was identified whose activity could be affected by high glucose level, and indirectly influence the levels of some PcWdes. The analysis of the PcWdes could help to understand part of the fungal infection process and possibly find substances that can control its development.

关键词： carbohydrate-degrading enzymes Phytopathogenic Fungi Smut Fungi Transcriptome Thecaphora frezii

Artificial Triterpenoid Fatty Acid Ester Isolated From the Leaves of Phytolacca icosandra L

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Natural Products and Bioprospecting 2020年第4期10卷 221-225页

作者： Elier Galarraga Andersson Mavares Neudo Urdaneta Rafael ERodríguez-Lugo Juan Manuel Amaro-Luis departamento de Química.Edificio de Química y procesos Universidad Simón Bolívar(USB)Apartado 89000.Caracas-1080AVenezuelaUSA Laboratorio de Química Bioinorgánica Centro de QuímicaInstituto Venezolano de Investigaciones Científicas(IVIC)VenezuelaCaracas 1020-AUSA Laboratorio de productos Naturales Departamento de Química.Facultad de CienciasUniversidad de Los Andes(ULA)MéridaVenezuela-5101USA

The methanol extract form the leaves of Phytolacca icosandra L.,afforded the unprecedented artificial triterpenoid fatty acid ester 1 derived from the new natural triterpenoid phytolaccagenic acid 3-O-myristate(1a),along with the three known triterpenoids serjanic,acinosolic and phytolaccagenic acid(2-4).Their structures were stablished by HR-EI-MS,1D and 2D NMR *** possible mechanistic formation of 1 is proposed,and the in vitro toxicity of all compounds was assessed using the brine shrimp lethality assay(BSLA).

关键词： Phytolacca icosandra.triterpenoid fatty acid ester NMR artificial products BSLA

cysA,cysP,and rpoS mutations increase the power density in *** microbial fuel cells:Performing enhancement based on metabolic flux analysis

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Advances in Bioscience and Biotechnology 2013年第1期4卷 103-111页

作者： Juan Diego Mejia cindy Stephany Rojas Laura Avellaneda Franco David Alejandro Urbina Gomez Beatriz Helena correa Arias Nubia Milena Velasco Rodriguez Maria Teresa cortes Montanez Martha Josefina Vives-Florez Andres Fernando Gonzalez Barrios Grupo de Diseno de productos y procesos(GDPP) Departamento de Ingenieria QuimicaUniversidad de los AndesBogotaColombia Grupo de Investigacion en Produccion y Logistica(PyLO) Departamento de Ingenieria IndustrialUniversidad de los AndesBogotaColombia Laboratorio de Electroquimica y Materiales Polimericos Departamento de QuimicaUniversidad de los AndesBogotaColombia centro de Investigaciones Microbiologicas(cIMIc) Departamento de Ciencias BiologicasUniversidad de los AndesBogotaColombia

This study presents the simulation of a MFc with Pseudomonas aeruginosa based on a metabolic flux analysis (MFA) which arises as a linear programming model that served as input for the fuel cell model. The linear model was implemented in Xpress MP? and the coupling model in comsol Multiphysics?. The in silico model predicted maximum potentials of 0.135 V which were consistent with those obtained in the experimental cell. Afterwards, an optimization platform based on multiobjective optimization approach was implemented aimed to determine genes that increase the average cell power. cysA, cysP and rpoS mutants which were predicted to increase the power of the cell, were experimentally tested with an air cathode fuel cell finding an augment up to 35-fold in the average power density for the rpoS mutant. Power densities were obtained through experimentation in the range of [0.13 - 5] mW/m2. Electron shuttle rise was qualitative corroborated trough cyclic voltammetry tests, which allowed to visualize the augment of the peaks for rpoS mutation.

关键词： component Formatting Style Styling Insert

Foaming supramolecular surfactants for gas mobility control in naturally fractured carbonate reservoirs at high temperature,salinity,and hardness

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Petroleum Science 2022年第6期19卷 3134-3148页

作者： enrique Soto-castruita Raúl Hern andez-Altamirano Eduardo Buenrostro-González Erick-Emanuel Luna-Rojero Sung Jae Ko-Kim Violeta-yasmín Mena-cervantes Mirna Pons-Jiménez Jorge-Francisco Ramírez-Pérez David-Aaσn Nieto-Alvarez Ricardo cerón-camacho Jos e-Ernesto Parra Raúl Oviedo-Roa Jos e-Manuel Martínez-Magadán Rodolfo cisneros-Dévora Luis-S.Zamudio-Rivera Instituto Mexicano del Petr oleo Eje Central Lazaro Cardenas Norte 152Col.San Bartolo AtepehuacanCiudad de Mexico07730Mexico cONAcy T-Instituto Mexicano del Petroleo Eje Central Lazaro Cardenas Norte 152Col.San Bartolo AtepehuacanCiudad de Mexico07730Mexico centro Mexicano para la Produccion mas Limpia Instituto Politecnico NacionalAvenida Acueducto s/nCol.La Laguna TicomanCiudad de Mexico07340Mexico centro de Tecnologías para Exploracion y Produccion Camino de Terracería 800Col.San Jose NovilleroBoca delRíoVeracruz94286Mexico

Oil production and mainte nance are essential issues in naturally fractured reservoirs because they are the largest and most productive on ***,they present early water and gas channeling but could be remediated by using foaming agents to control these phenomena through blocking channeling *** Mexico these reservoirs have pressure up to 5,500 psi,high temperature up to 200℃,salinity up to400,000 ppm,and hardness up to 250,000 ppm;due to these thermodynamic conditions,there has been no available technology to form stable enough *** this work,a foaming supramolecular surfactant with the capability to chelate ca^(2+)ions is *** a result,surfactant monomers are bridged by captured ca^(2+)cations leading to the formation of high-molecular-weight oligomers,which significantly increment the viscosity of the solution improving the foam stability,and since at this manner the ca2+cations are no longer available to precipitate as components of solid salts,the foaming supramolecular surfactant also performs as *** observations are explained through quantum theoretical *** foam is stable,effectively blocking the gas channels,whereas in presence of oil the foam is broken leading the oil to pass into the *** characteristic rheological properties of the foam allow its injection into the formation at a range of flow rates,foam qualities,and shear stress to achieve the flooding and the blocking of a variety of fractured carbonate formations,and the change of the wettability of the matrix,which is a desirable behavior in a huff and puff process,as reported in a previous publication about a successful pilot test of this foam.

关键词： calcium chelation Foaming composition Supramolecular surfactant Molecular design Gas mobility control Flow assurance Improved oil recovery conformance control