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Proteomics for discovery of candidate colorectal cancer biomarkers

World Journal of Gastroenterology 2014年第14期20卷 3804-3824页

作者： Paula lvarez-Chaver Olalla Otero-Estévez María Páez de la Cadena Francisco J Rodríguez-Berrocal Vicenta S Martínez-Zorzano Unidad de Proteómica Servicio de Determinación EstructuralProteómica y GenómicaCentro de Apoyo Científico y Tecnológico a la InvestigaciónUniversidad de VigoCampus As Lagoas-Marcosende área de Bioquímica y biología Molecular Departamento de BioquímicaGenética e InmunologíaFacultad de BiologíaUniversidad de VigoCampus As Lagoas-Marcosende

Colorectal cancer(CRC)is the second most common cause of cancer-related deaths in Europe and other Western countries,mainly due to the lack of wellvalidated clinically useful biomarkers with enough sensitivity and specificity to detect this disease at early *** it is well known that the pathogenesis of CRC is a progressive accumulation of mutations in multiple genes,much less is known at the proteome ***,in the last years many proteomic studies have been conducted to find new candidate protein biomarkers for diagnosis,prognosis and as therapeutic targets for this malignancy,as well as to elucidate the molecular mechanisms of colorectal *** important advantage of the proteomic approaches is the capacity to look for multiple differentially expressed proteins in a single *** review provides an overview of the recent reports describing the different proteomic tools used for the discovery of new protein markers for CRC such as two-dimensional electrophoresis methods,quantitative mass spectrometry-based techniques or protein ***,we will also focus on the diverse biological samples used for CRC biomarker discovery such as tissue,serum and faeces,besides cell lines and murine models,discussing their advantages and disadvantages,and summarize the most frequently identified candidate CRC markers.

关键词： Colorectal cancer Serum Tissue Proteins Biomarkers Proteomics Protein identification Mass spectrometry

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Trypanosoma evansi: A Qualitative and Quantitative Ultrastructural Analysis of the Spleen during Experimental Murine Infections

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Microscopy Research 2020年第3期8卷 31-42页

作者： Lucia Proietti d’Empaire Félix Tejero Hector J. Finol Pedro M. Aso Antonio Roschman-Gonzalez centro de biología estructural Instituto Venezolano de Investigaciones Científicas Caracas Venezuela Instituto de Zoología y Ecología Tropical Facultad de Ciencias Universidad Central de Venezuela Caracas Venezuela centro de Microscopía Electrónica Facultad de Ciencias Universidad Central de Venezuela Caracas Venezuela departamento de biología Celular Universidad Simón Bolívar Caracas Venezuela

A murine model is used to study qualitatively and quantitatively the splenic ultrastructural changes induced by two Trypanosoma evansi strains derived from naturally infected local equine hosts (Equusasinus and E. caballus);T. evansi causes ultrastructural modifications in the spleen of the infected mice. The modifications include tissular disorganization, fibrosis, mitochondrial swelling, apoptosis and necrosis. The initial phases of the infection are quite similar, whereas the final phases differ qualitatively depending on the strain’s source. The ultrastructural quantitative changes were studied in the reticular splenocytes covering alterations in the area of the cytoplasm and nucleus. Analysis of the results shows the induction of various splenic alterations caused by local T. evansi strains. Also, it was documented that discriminative time modulation, as well as progressive tissular, cellular and subcellular changes, are more associated with derived infections from E. caballus strain.

关键词： Trypanosoma evansi Spleen Ultrastructure Mouse

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In Silicoand in VitroApproach for the Understanding of the Xanthine Oxidase Inhibitory Activity of Uruguayan Tannat Grape Pomace and Propolis Poliphenols

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Journal of Biophysical Chemistry 2019年第1期10卷 1-14页

作者： Elena Alvareda Federico Iribarne Victoria Espinosa Pablo Miranda Daniela Santi Sara Aguilera S. Bustos Margot Paulino Zunini centro de Bioinformática estructural DETEMA Facultad de Química Universidad de la República Montevideo Uruguay centro Universitario Región Noroeste Sede Salto Universidad de la República Salto Uruguay centro de Investigaciones Biomédicas y Aplicadas Escuela de Medicina Facultad de Ciencias Médicas Universidad de Santiago de Chile Santiago Chile departamento de Ciencias Farmacéuticas Facultad de Ciencias Químicas Universidad Nacional de Córdoba Ciudad Universitaria Córdoba Argentina Instituto Multidisciplinario de biología Vegetal (IMBIV-CONICET) Ciudad Universitaria Córdoba Argentina Faculty of Sciences Universidad Católica del Norte Antofagasta Chile

The use of food additives with xanthine oxidase (XO) inhibitory activity offers an alternative approach to hyperuricemic and gout disease treatment, and provides an example of antioxidant nutraceutics. The in vitro and in silico XO inhibitory activity of polyphenols from Uruguayan Tannat grape pomaces and propolis extracts was evaluated as well as the scavenging capacity of said compounds. When comparing propolis and grape pomace samples, the in vitro studies demonstrated that polyphenols extracted from propolis are more active as free radical scavengers than those from Tannat grape pomace. Both natural products effectively inhibited XO but the capacity of phenols present in GP is higher than the one present in P. The high content of anthocyanins in GP, absent in P, could account for this observation. In silico assays allowed us to determine relevant ligand-receptor interactions between polyphenols, from a database built with previously reported polyphenols from both natural products, and the active site of XO. The in silico results showed that compound (E)-isoprenylcaffeate from propolis was the best potential XO inhibitor displaying hydrophobic aromatic interaction between the conjugated ring of the caffeate moiety and polar interactions between hydroxyl groups from caffeate with the active site polar residues. Among grape pomaces, the Cyanidin-3-O-(6-(E)-p-coumaroyl)-glucoside was the best XO inhibitor;its moiety oxychromenyl being relevant to the docking stabilization. All these results lead us to propose Uruguayan propolis and Tannat grape pomace extracts as food additives as well as phytopharmaceuticals to decrease the uric acid levels in gout disease and to act against oxidative stress.

关键词： Propolis Tannat Grape Pomace Xanthine Oxidase Inhibition Polyphenols Functional Foods

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Linde Type a Zeolite and Type Y Faujasite as a Solid-Phase for Lead, Cadmium, Nickel and Cobalt Preconcentration and determination Using a Flow Injection System Coupled to Flame Atomic Absorption Spectrometry

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American Journal of Analytical Chemistry 2013年第8期4卷 387-397页

作者： Yaneira Petit de Pena Wendy Rondón Laboratorio de Espectroscopía Molecular Facultad de CienciasUniversidad de Los AndesMéridaVenezuela Laboratorio de Química Analítica Centro de QuímicaInstituto Venezolano de Investigaciones CientíficasCaracasVenezuela

In this work, a flow injection analysis (FIA) method for the trace determination of lead, cadmium, nickel and cobalt in natural waters by formation of neutral chelates with ammonium pyrrolidine dithiocarbamate (APDC) was developed. The neutral chelates formed was retained in a mini-column packed with Linde type A zeolite (LTA) and type Y Faujasite zeolite (FAU) and then eluted with methyl isobutyl ketone (MIBK) to flame atomic absorption spectrometry (EAA) for its detection. Physicochemical characterization of this zeolite was carried out by Fourier Transform infrared spectroscopy and attenuated total reflectance (FTIR and IR-ATR), scanning electron microscopy and energy dispersive X-ray microanalysis (SEM-EDX) and X-ray power diffraction (XRD). Then, a FIA configuration was used with a column preconcentration system coupled to the detection system at room temperature (22?C). The detection limit and the relative standard deviation for 5 determinations of different solutions of Pb2+, Co2+, Ni2+ and Cd2+ for FAU and LTA zeolite were calculated. The sampling frequency ranged from 18-35 h-1 and preconcentration factors from 21-250 were achieved, for a sample volume of 6 mL using 20 mg of sorbents, indicating a high retention of the analytes on the zeolites material. The recoveries obtained in natural waters samples were close to 100% for all ions metal using synthetic zeolites, confirming the applicability of the method. The isotherm models of Langmuir, Scatchard, Freundlich and Dubinin-Radushkevich were used to study the equilibrium data, indicating that successfully followed the Freundlich and Dubinin-Radushkevich (D-R) isotherms at low metal ion concentration. The Freundlich parameter n varied between 0.35-1.01, whereas D-R isotherm yields the sorption free energy E 8 ***-1 indicating psysisorption.

关键词： Cobalt Nickel Cadmium Lead Flow Injection Preconcentration Zeolites Atomic Absorption

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