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Molecular cloning and characterization of the endothelin 3 gene in black bone sheep

Journal of Animal Science and Biotechnology 2018年第4期9卷 847-857页

作者： Hesham Y.A.Darwish Yuanyuan Zhang Kai Cui Zu Yang Deping han Xianggui Dong Huaming Mao Weidong Deng Xuemei Deng National Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics Breeding and Reproduction of the Ministry of AgricultureChina Agricultural University Animal Production research Institute Agricultural Research Center Ministry of Agriculture and Land Reclamation College of Veterinary Medicine China Agricultural University College of Animal Science and Technology Yunnan Agricultural University

Background: Black bone sheep was first discovered in Yunnan province of China in 1970, with unique black pigmentation on the body and internal organs. Endothelin 3(EDN3) has been known as a key gene causing hyperpigmentation in black bone chicken, the Silky ***: In this study, EDN3 was employed as a candidate gene for regulating black color pigmentation. First,EDN3 was cloned from sheep to obtain the full-length cDNA by using the rapid amplification of cDNA ends(RACE).Genomic EDN3 was screened and a total of thirty predicted single nucleotide polymorphisms(SNPs) were genotyped for allele and genotype frequency analysis in a case-control study involving two black bone sheep populations. Genomic copy number analysis of EDN3 in sheep was conducted to measure the variation in copy number. EDN3 expression levels were observed among the groups in adult liver, lymph node, and kidney tissues, as well as embryo kidney samples. Also, among the tissues of black bone and non-black bone ***: The size of the full-length cDNA was 1,578 bp, which included 426 bp of 5′-untranslated region(5′-UTR),an open reading frame(ORF) of 639 bp encoding a protein of 212 amino acids, and a 3′-UTR of 513 bp. Genotype and allele frequencies of all the discovered SNPs were found insignificantly different in black bone and non-black bone sheep(P > 0.05). Genomic copy number analysis of EDN3 in sheep revealed no significant difference between the two sheep groups. No significant variations were found in the adult liver and kidney embryo samples. However,the expression in lymph node and kidney tissue was significantly higher in black bone sheep than that in non-black bone sheep(P < 0.05). Significant variations in the EDN3 expression levels were observed among the tissues of nonblack bone ***: The findings of the present study indicate that unlike in Silky chickens, EDN3 is not responsible for hyperpigmentation but may play a key functional role in immune and excretory

关键词： Black pigmentation Expression level Sequence analysis SNP

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Characterization of a Novel Gene, srpA, Conferring Resistance to Streptogramin A, Pleuromutilins, and Lincosamides in Streptococcus suis

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Engineering 2022年第2期8卷 85-94页

作者： Chaoyang Zhang Lu Liu Peng Zhang Jingpo Cui Xiaoxia Qin Lichao Ma Kun han Zhanhui Wang Shaolin Wang Shuangyang Ding Zhangqi Shen College of Veterinary Medicine China Agricultural UniversityBeijing 100193China Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety and Beijing Laboratory for Food Quality and Safety China Agricultural UniversityBeijing 100193China

Antimicrobial resistance is undoubtedly one of the greatest global health threats. The emergence of multidrug-resistant(MDR) Gram-positive pathogens, such as methicillin-resistant Staphylococcus aureus(MRSA), vancomycin-resistant Enterococcus faecium(VRE), and β-lactamase-resistant Streptococcus pneumonia, has severely limited our antibiotic arsenal. Numerous ribosome-targeting antibiotics, especially pleuromutilins, oxazolidinones, and streptogramins, are viewed as promising alternatives against aggressive MDR pathogens. In this study, we identified a new adenosine triphosphate(ATP)-binding cassete(ABC)-F family determinant, srp A, in Streptococcus suis(S. suis) by means of a comparative analysis of the whole-genome sequences of tiamulin(TIA)-resistant and TIA-sensitive bacteria. Functional cloning confirmed that the deduced gene can mediate cross-resistance to pleuromutilins, lincosamides, and streptogramin A in S. suis and S. aureus. A sequence alignment revealed that Srp A shares the highest amino acid identity with Vga(E)(36%) and shows canonical characteristics of ABC-F family *** Srp A-ribosome docked compounds, the extended loop region of Srp A approaches the valnemulinbinding pocket in the ribosome peptidyl-transferase center and competes with bound valnemulin. A detailed mutational analysis of the loop residues confirmed that this domain is crucial for Srp A activity,as substitutions or truncations of this region affect the efficiency and specificity of antibiotic *** antibiotics accumulation indicated that Srp A does not act as an efflux pump, while a ribosome binding assay supported the protective effects of Srp A on the ribosome by preventing antibiotic binding as well as displacing bound drugs. These findings clarify the mechanisms underlying resistance to ribosomal antibiotics.

关键词： SrpA Streptococcus suis Antibiotic resistance Ribosome ABC-F family proteins

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History, current status, and prospects of soybean production and research in sub-Saharan Africa

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The Crop Journal 2018年第3期6卷 226-235页

作者： Dalia Mohamedkheir Khojely Seifeldin Elrayah Ibrahim Enoch Sapey Tianfu han MOA Key Lab of Soybean Biology (Beijing) Institute of Crop SciencesChinese Academy of Agricultural Sciences Gezira research Station Agricultural Research Corporation (ARC) Oil Palm Breeding and Genetics CSIR-Oil Palm Research Institute

Soybean(Glycine max(L.) Merr.) is a non-native and non-staple crop in sub-Saharan Africa(SSA) with potential to be a commercial crop owing to its wide range of uses as food, feed,and industrial raw material. Soybean was first introduced to SSA by Chinese traders in the19 th century and was cultivated as an economic crop as early as 1903 in South Africa. In the past four decades, soybean cultivation area and production in SSA has increased exponentially, from about 20,000 ha and 13,000 t in the early 1970 s to 1,500,000 ha and2,300,000 t in 2016. Soybean yield has been stagnant in SSA for decades at about 1.1 t ha^(-1),much lower than the world average, representing one of the most challenging issues in the soybean industry in SSA. The low soybean yield in SSA can be attributed to the use of poorperforming varieties and to the limited application of fertilizers and rhizobial inoculants in soils with no history of soybean production. South Africa, Nigeria, Zambia, and Uganda are the leading soybean producers in SSA. Soybean research in SSA is conducted by international and national research institutions, including IITA, national soybean improvement programs, universities, and the private sector. Between 1970 and 2011, 195 soybean varieties were released by IITA, private breeders, and national soybean improvement programs in SSA. This paper reviews the history and current state of soybean production and of the utilization and adoption of tropical varieties in SSA, addresses the major soybean yield-limiting factors across the region, and discusses the potential of the soybean industry in SSA. It also highlights soybean improvement efforts and lessons learned from previous soybean improvement efforts and the current progress of some national soybean improvement programs in SSA. Opportunities for scaling up tropical soybean as a major crop across SSA countries are promising.

关键词： Soybean Sub-Saharan Africa(SSA) History Current status Prospects

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Specification and grade of Saposhnikoviae Radix(Saposhnikovia divaricata)

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Chinese Herbal Medicines 2022年第4期14卷 543-553页

作者： Lijuan Lyu Xing Li Erhuan Zang Yumei Yan Min Yang Wenle Wang Chunhong Zhang Minhui Li Department of Basic Science Tianjin Agricultural UniversityTianjin 300384China Inner Mongolia Autonomous region Hospital of Traditional Chinese Medicine Hohhot 010020China Baotou Medical College Baotou 014040China Inner Mongolia Key Laboratory of Characteristic Geoherbs Resources and Utilization Baotou Medical CollegeBaotou 014040China

Objective:Saposhnikoviae Radix(Fangfeng in Chinese),the roots of Saposhnikovia divaricata,lacks commodity specification and grade standardization in the current *** study investigated the existing specifications and grades of Saposhnikoviae Radix to provide a standardized scientific reference for its market ***:Based on a textual research of Chinese herbal medicine from the han Dynasty to the present,medicinal materials of different specifications and grades obtained from Saposhnikoviae Radix in the main producing areas of China were collected and the markets for these materials were *** investigations were performed in the major producing areas such as Northeast China,Hebei Province,and Inner *** major Chinese herbal medicine markets in China were *** indices were used to categorize the two specifications(wild and cultivated)according to the shape,color,texture,and ***-performance liquid chromatography was performed to determine the active *** calipers and measuring tape were used to measure the diameter and length,respectively,of 41 *** Excel and the R Language software,cluster analysis and descriptive statistical analysis were performed to assist in the application of new specifications and grades based on physical characteristics,pharmacological activity,and chemical ***:The two specifications(wild and cultivated)of Saposhnikoviae Radix were divided into three grades each based on the length and ***-O-glucosylcimifugin,5-O-methylvisamminoside,and the length of Saposhnikoviae Radix can be used as a basis for classifying the commodity specifications and *** specifications and grade standards of Saposhnikoviae Radix were established based on the following eight aspects:shape,surface characteristics,texture,cross section,taste,prim-Oglucosylcimifugin content,5-O-methylvisamminoside content and ***:The formulation of this st

关键词： classification commodity specification and grade market Saposhnikovia divaricata(Turcz.)Schischk Saposhnikoviae Radix production area

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Fine-mapping and characterisation of genes on barley(Hordeum vulgare)chromosome 2H for salinity stress tolerance during germination

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The Crop Journal 2022年第3期10卷 754-766页

作者： Edward Mwando Yong han Tefera Angessa Xiao-Qi Zhang Chengdao Li Western Crop Genetics Alliance College of ScienceHealthEngineering and EducationMurdoch UniversityMurdochWA 6150Australia Western Australian State agricultural Biotechnology centre Murdoch UniversityMurdochWA 6150Australia Department of Primary Industries and regional Development 3 Baron-Hay CourtSouth PerthWA 6151Australia

Salinity causes a detrimental impact on plant growth,particularly when the stress occurs during germination and early development *** is one of the most salt-tolerant crops;previously we mapped two quantitative trait loci(QTL)for salinity tolerance during germination on the short arm of chromosome 2 H using a CM72/Gairdner doubled haploid(DH)***,we narrowed down the major QTL to a region of 0.341 or 0.439 Mb containing 9 or 24 candidate genes belonging to 6 or 20 functional gene families according to barley reference genomes v1 and v3 respectively,using two DH populations of CM72/Gairdner and Skiff/CM72,F_(2)and F;generations of CM72/Gairdner/;Spartacus CL,Two Receptorlike kinase 4(RLPK4)v1 or Receptor-like kinase(RLK)v3 could be the candidates for enhanced germination under salinity stress because of their upregulated expression in salt-tolerant variety ***,several insertion/deletion polymorphisms were identified within the 3 rd exon of the genes between CM72 and *** sequence variations resulted in shifted functional protein domains,which may be associated with differences in salinity *** molecular markers were designed for selecting the locus with receptor-like protein kinase 4,and one was inside HORVU2 Hr1 G111760.1 or ***.r3.2 *** diagnostic markers will allow for pyramiding of 2 H locus in barley varieties and facilitate genetic improvement for saline ***,validation of the genes to elucidate the mechanisms involved in enhancing salinity tolerance at germination and designing RLPK4 specific markers is *** this publication,all the analysis was based on barley reference genome of2017(v1),and it was used throughout for ***,the positions of the markers and genes identified were updated according to new genome(v3)for reference.

关键词： Barley Germination Salinity tolerance Diagnostic markers Receptor-like protein kinase 4 Gene expression Sequence analysis

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拟南芥NHX5和NHX6调节PIN5介导的生长发育过程

拟南芥NHX5和NHX6调节PIN5介导的生长发育过程

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2018全国植物生物学大会

作者： Ligang Fan Lei Zhao Wei Hu Weina Li OndrejNovak Miroslav Strnad Sibu Simon Jiri Friml Jinbo Shen Liwen Jiang Quan-Sheng Qiu MOE Key Laboratory of Cell Activities and Stress Adaptations School of Life SciencesLanzhou University Laboratory of Growth Regulators Centre of the Region Hand for Biotechnological and Agricultural ResearchInstitute of Experimental Botany AS CR & Palacky University Mendel centre for Plant Genomics and Proteomics Central European Institute of TechnologyMasaryk University

拟南芥NHX5和NHX6属于内膜Na,K反向交换体,定位于Golgi、TGN和MVB/PVC。研究表明,NHX5和NHX6在调控细胞Na、K和pH平衡,蛋白运输以及耐盐胁迫和钾营养等方面起着重要的作用。我们实验室前期研究发现,nhx5 nhx6双突变体生长发育受阻;nhhx5...

拟南芥NHX5和NHX6属于内膜Na,K反向交换体,定位于Golgi、TGN和MVB/PVC。研究表明,NHX5和NHX6在调控细胞Na、K和pH平衡,蛋白运输以及耐盐胁迫和钾营养等方面起着重要的作用。我们实验室前期研究发现,nhx5 nhx6双突变体生长发育受阻;nhhx5 nhx6双突变体莲座叶小、植株矮、开花抽苔推迟、种子产量降低,表明NHX5和NHX6调控植物的生长发育。然而,目前关于NHX5和NHX6调控植物生长发育的分子机理尚无

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Characterization and Pathogenicity of Pseudopestalotiopsis vietnamensis Causing Gray Blight of Wuyi Rock Tea(Camellia sinensis)in China and Specific Mechanisms of Disease Infection

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Phyton-International Journal of Experimental Botany 2023年第1期92卷 131-147页

作者： Guangheng Wu Lu Rui Xiang Lu Libo han Gan Lv Xianyu Fu Jinxian Liu Nong Zhou Chuanhai Zhang Fujian Provincial Key Laboratory of Eco-Industrial Green Technology College of Ecology and Resources EngineeringWuyi UniversityWuyishan354300China Key Laboratory of Ministry of Education for Genetics Breeding and Multiple Utilization of CropsPlant Immunity CenterFujian Agriculture and Forestry UniversityFuzhou350002China Chongqing Engineering Laboratory of Green Planting and Deep Processing of Famous-region Drug in the Three Gorges Reservoir region College of Biology and Food EngineeringChongqing Three Gorges UniversityChongqing404120China State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural UniversityChengdu611130China College of Tea and Food Science Wuyi UniversityWuyishan354300China

Gray blight disease(GBD)causes significant losses in tea production in *** genes and biological processes involved in resistance to fungal disease in tea plants have been identified,specific mechanisms of the GBD infection process remain *** this study,morphological and multi-gene(TEF-TUB-ITS)phylogenetic characteristics were used to identify isolate CLBB1 of Pseudopestalotiopsis *** tests confirmed that isolate CLBB1 from tea leaves caused GBD in the susceptible tea cultivar Wuyi Rock(Camellia sinensis *** ***).Spores began to germinate 24 h after infection(hai),and after 48 h,elongated fungal hyphae formed from a single *** analysis revealed that 482,517,and 369 genes were differentially expressed at 24,48,and 72 hai,respectively,in Wuyi Rock tea *** CLBB1 infection elicited phenotype-related responses and activated defense-related pathways,including plant–pathogen interaction,MAPK signaling,and plant hormone signal transduction,suggesting a possible mechanism underlying phenotype-based susceptibility to ***,a new *** strain causing GBD in the tea cultivar‘Shuixian’was discovered in this *** analysis indicated that pathogen invasion activated chitin-related MAPK pathways and that tea plants required a hormone to restrict CLBB1.

关键词： Chitin-related MAPK pathways gray blight disease Pseudopestalotiopsis tea transcriptional analysis

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Map-based cloning and CRISPR/Cas9-based editing uncover BoNA1 as the causal gene for the no-anthocyanin-accumulation phenotype in curly kale (Brassica oleracea var. sabellica)

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Horticulture research 2023年第8期10卷 194-202页

作者： Kaiwen Yuan Xinyu Zhao Wenru Sun Limei Yang Yangyong Zhang Yong Wang Jialei Ji Fengqing han Zhiyuan Fang Honghao Lv State Key Laboratory of Vegetable Biobreeding Institute of Vegetables and FlowersChinese Academy of Agricultural SciencesBeijing100081China

Brassica oleracea comprises several important vegetable and ornamental crops,including curly kale,ornamental kale,cabbage,broccoli,and *** accumulation of anthocyanins,important secondary metabolites valuable to human health,in these plants varies widely and is responsible for their pink to dark purple *** curly kale varieties lack anthocyanins,making these plants completely *** genetic basis of this trait is still *** crossed the curly kale inbred line BK2019(without anthocyanins)with the cabbage inbred line YL1(with anthocyanins)and the Chinese kale inbred line TO1000(with anthocyanins)to generate segregating *** no-anthocyanin trait was genetically controlled by a recessive gene,*** generated a linkage map and mapped bona1 to a 256-kb interval on *** identified one candidate gene,Bo9g058630,in the target genomic region;this gene is homologous to AT5G42800,which encodes a dihydroflavonol-4-reductase-like(DFR-like)protein in *** BK2019,a 1-bp insertion was observed in the second exon of Bo9g058630 and directly produced a stop *** verify the candidate gene function,CRISPR/Cas9 gene editing technology was applied to knock out *** generated three bona1 mutants,two of which were completely green with no anthocyanins,confirming that Bo9g058630 corresponds to *** insertion/deletion mutations in BoNA1 exons were found in all six of the other no-anthocyanin kale varieties examined,supporting that independent disruption of BoNA1 resulted in no-anthocyanin varieties of *** study improves the understanding of the regulation mechanism of anthocyanin accumulation in *** subspecies.

关键词： crops fir insertion

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Identification of Geographical Origins for Blackcurrant Cultivars Based on Amino Acid Compositions

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Journal of Northeast agricultural University(English Edition) 2023年第3期30卷 69-84页

作者： Ren Chuan-kun Xing Li-wei Li Wen-hui Li Xing-guo Yu Ze-yuan Wang Xiao-tian Li Ying-mei Shao Bang-tao han De-guo Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Northeast region) Ministry of Agricultureand Rural AffairsCollege of Horticulture&Landscape ArchitectureNortheast Agricultural UniversityHarbin 150030China National-Local Joint Engineering research Center for Development and Utilization of Small Fruits in Cold regions Harbin 150030China Department of Intelligent Agriculture Heilongjiang Agricultural Economy Vocational CollegeMudanjiang 157041HeilongjiangChina

In this study,a total of 36 blackcurrant(Ribes nigrum L.)cultivars grown in the Northeast of China were selected,including 12 cultivars introduced from Russia,10 from Poland and the rest from local *** physicochemical properties and amino acid compositions of these varieties were studied,and the geographical origins of blackcurrants were tracked by multivariate statistical analysis.A total of 23 amino acids were detected in all cultivars,which were rich in glutamine,glutamate,aspartate,asparagine,α-alanine,γ-aminobutyric acid,valine and *** content of the total amino acids in these cultivars was from 31.21 mg•100 g-1 to 319.40 mg•100 *** linear discriminant analysis(SLDA)was introduced to perform satisfactory categorization for blackcurrant cultivars,which achieved a success rate of 88.9%for the identification of geographical *** results suggested that the compositions of amino acids in blackcurrants could effectively predict geographical origins.

关键词： blackcurrant amino acid HPLC stepwise linear discriminant analysis

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Understanding divergent domestication traits from the whole-genome sequencing of swamp-and river-buffalo populations

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National Science Review 2020年第3期7卷 686-701页

作者： Xier Luo Yu Zhou Bing Zhang Yi Zhang Xiaobo Wang Tong Feng Zhipeng Li Kuiqing Cui Zhiqiang Wang Chan Luo Hui Li Yanfei Deng Fenghua Lu Jianlin han Yongwang Miao Huaming Mao Xiaoyan Yi Cheng Ai Shigang Wu Alun Li Zhichao Wu Zijun Zhuo Do Da Giang Bikash Mitra Mohammad Farhad Vahidi Shahid Mansoor Sahar Ahmed Al-Bayatti Eka Meutia Sari Neena Amatya Gorkhali Sigit Prastowo Laiba Shafique Guoyou Ye Qian Qian Baoshan Chen Deshun Shi Jue Ruan Qingyou Liu State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources Guangxi University CAS Key Laboratory of Genome Sciences and Information Beijing Institute of Genomics Chinese Academy of Sciences National Engineering Laboratory for Animal Breeding Key Laboratory of Animal Genetics Breeding and Reproduction of Ministry of Agriculture and Rural AffairsCollege of Animal Science and Technology China Agricultural University Guangdong Laboratory for Lingnan Modern Agriculture Genome Analysis Laboratory of the Ministry of Agriculture Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural Sciences CAAS-ILRI Joint Laboratory on Livestock and Forage Genetic Resources Institute of Animal ScienceChinese Academy of Agricultural Sciences International Livestock research Institute Faculty of Animal Science and Technology Yunnan Agricultural University Bacgiang Agriculture and Forestry University Cellular Immunology Lab Department of Zoology University of North Bengal Animal Biotechnology Department Agricultural Biotechnology Research Institute of Iran-North Region 11. National Institute for Biotechnology and Genetic Engineering 12. Animal Genetic Sources DepartmentDirectorate of Animal Resources Ministry of Agriculture 13. Department of Animal Science Faculty of Agriculture Syiah Kuala University 14. Animal Breeding DivisionNational Animal Science Research Institute Nepal Agriculture Research Council 15. Animal Science Department Universitas Sebelas Maret 16. International Rice Research Institute

Domesticated buffaloes have been integral to rice-paddy agro-ecosystems for millennia, yet relatively little is known about the buffalo genomics. Here, we sequenced and assembled reference genomes for both swamp and river buffaloes and we re-sequenced 230 individuals(132 swamp buffaloes and 98 river buffaloes)sampled from across Asia and Europe. Beyond the many actionable insights that our study revealed about the domestication, basic physiology and breeding of buffalo, we made the striking discovery that the divergent domestication traits between swamp and river buffaloes can be explained with recent selections of genes on social behavior, digestion metabolism, strengths and milk production.

关键词： Bubalus bubalis genome buffalo domestication re-sequencing traits

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