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Spatiotemporal Dynamics of the BRI1 Receptor and its Regulation by Membrane Microdomains in Living Arabidopsis Cells

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Molecular Plant 2015年第9期8卷 1334-1349页

作者： Li Wang Hong Li Xueqin Lv Tong Chen Ruili Li Yiqun xuei Jianjun Jiang Biao Jin Frantisek Baluska Jozef Samaj Xuelu Wang Jinxing Lin Key Laboratory of Ptant Resources Institute of Botany Chinese Academy of Sciences Beijing 100093 China College of Horticulture and Plant Protection Yangzhou University Yangzhou 225009 China College of Biological Sciences and Biotechnology Beijing Forestry University Beijing 100083 China State Key Laboratory of Genetic Engineering and Institute of Plant Biology School of Life Sciences Fudan University Shanghai 200433 China Institute of Cellular and Molecular Botany University of Bonn Kirschallee 1 D-53115 Bonn Germany Department of Cell Biology Palacky University OIomouc Olomouc 78371 Czech Republic College of Life Science and Technology Huazhong Agricultural University Wuhan 430070 China These authors contributed equally to the article.

The major brassinosteroid （BR） receptor of Arabidopsis BRASSINOSTEROID INSENSITIVE1 （BRI1） plays fundamental roles in BR signaling, but the molecular mechanisms underlying the effects of BR on BRI1 internalization and assembly state remain unclear. Here, we applied variable angle total internal reflection fluorescence microscopy and fluorescence cross-correlation spectroscopy to analyze the dynamics of GFP-tagged BRII. We found that, in response to BR, the degree of co-localization of BRI1-GFP with AtFIotl-mCherry increased, and especially BR stimulated the membrane microdomain-associated pathway of BRI1 internalization. We also verified these observations in endocytosis-defective chc2-1 mutants and the AtFIotl amiRNA 15-5 lines. Furthermore, examination of the phosphorylation status of bril-EMS-suppressor 1 and measurement of BR-responsive gene expression revealed that membrane microdomains affect BR signaling. These results suggest that BR promotes the partitioning of BRI1 into functional membrane microdomains to activate BR signaling.

关键词： BRI1 BR signaling endocytosis membrane microdomains spatiotemporal dynamics

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Cloning,Sequencing and Analysis of DDX3Y Gene in Five Male Equus Animals

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agricultural Biotechnology 2015年第4期4卷 64-69页

作者： Huamin GUO Yanglin CHEN Shuyu LI Hongmei han Jindun ZhanG Lihong FAN Lixia ZHAO Xihe LI research Center for Animal Genetic Resources of Mongolia Plateau Inner Mongolia University Inner Mongolia Saikexing Reproductive Biotechnology Co Ltd.

[ Objective] This study analyzed the differences in DDX3 Y gene sequences among male horse, male donkey, male mule, male hinny and male foal to investigate the relationship between DDX3Y gene structure and the mechanism of infertility in male Equus fetus × asirms. [ Method] Partial sequences of DDX3Y gene in male horse, male donkey, male mule, male hinny and male foal were obtained by genome comparison, sequencing and cloning, and compared by bioinformatics methods. [ Result] The CDS regions varied in 5 base pairs and two amino acids. DDX3Y protein was predicted to be an unstable protein. In addition, the results also showed that DDX3 Y gene in male horse displayed a closer phylogenetic relationship to male mule and male foal; DDX3Y gene in male donkey displayed a closer phylogenetic relationship to male hinny. [ Conclusion] This study provided scientific basis for further exploring the function of DDX3 Y gene and the mecha- nism of reproductive regulation in male Equus ferus × asinus.

关键词： Male mule Male hinny Male foal DDX3Y Infertility

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Non-Camellia Tea in China: Traditional usage, phytochemistry, and pharmacology

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Chinese Herbal Medicines 2019年第2期11卷 119-131页

作者： Kai Ren Bi-qun han Li-juan Lv Guo-jun Zhang Lu-jing Lei Xiao-rong Bai Pei-gen Xiao Min-hui Li Baotou Medical College Baotou 014060 China Guangxi Botanical Garden of Medicinal Plants Nanning 530023 China Institute of Medicinal Plant Development Chinese Academy of Medical Sciences Peking Union Medical College Beijing 100193 China Department of Basic Science Tianjin Agricultural University Tianjin 300384 China Inner Mongolia Institute of Traditional Chinese Medicine Hohhot 010020 China

Non-Camellia Tea, as an integral part of Chinese tea culture for several centuries, is important to prevent chronic metabolic diseases. However, it was not systematically studied until academician Pei-gen Xiao defined it. Hereby, Non-Camellia Tea was reviewed systematically in definition, classification, traditional functions, chemical compositions, and pharmacology.

关键词： Non-Camellia Tea chemical compositions pharmacology traditional function

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The Effect of Nitrogen Deposition on Soil Enzyme Activity in the Forest Grassland Landscape Boundary of Tibet

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Journal of Landscape research 2018年第4期10卷 93-98页

作者： han Yanying LIU Yunlong YE Yanhui DA Buqiong GAO Yi ZHAO Yalei LI Linwei LIU Shuanghao Tibet Agriculture & Animal Husbandry University Linzhi Tibet 860000 China China agricultural University Beijing 100193 China Hunan University of Chinese Medicine Changsha Hunan 410208 China

The simulated nitrogen deposition [control check(CK), 0 kg·hm^2/a; low nitrogen(LN),25 kg·hm^2/a; medium nitrogen(MN), 50 kg·hm^2/a; high nitrogen(HN), 150 kg·hm^2/a] was performed from July 2014 to August 2015 in the forest-grassland boundary in Zhuqudeng Village, Bujiu Township,Linzhi City, Tibet Autonomous region to analyze the activity of enzymes(invertase, catalase, urease,amylase, cellulase, polyphenol oxidase, and β-glucosidase) in soil layers of 0-20 cm and 20-40 cm and explore the effect of different levels of nitrogen deposition on enzyme activity different layers of *** results showed that: ① different levels of simulated nitrogen deposition had remarkable effects on sucrase, amylase, cellulase, polyphenol oxidase and β-glucosidase in the soil layer of 0-20 cm(p 0.05); in the soil layer of 20-40 cm, the response made by sucrase, catalase, urease, amylase, cellulase, polyphenol oxidase and β-glucosidase to nitrogen deposition reached a significant level(p hanced under HN treatment, and the activity of invertase,catalase, amylase, cellulose, and β-glucosidase was inhibited by nitrogen deposition. ③ In the soil layer of20-40 cm, the activity of polyphenol oxidase and β-glucosidase reduced under under LN treatment and enhanced under HN treatment, and the activity of invertase, catalase, urease, amylase, and cellulase was inhibited by nitrogen deposition. ④ With the deepening of the boundary soil layer(from 0-20 cm to20-40 cm), urease and β-glucosidase made different responses to the different levels of nitrogen deposition,while invertase, catalase, amylase, cellulose, and polyphenol oxidase showed the same response to nitrogen deposition.

关键词：氮沉降土层雾霾环境保护

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Effect of Caragana korshinskii Silage at Different Growth Stages on the Feeding of Livestock

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Asian agricultural research 2023年第7期15卷 54-57页

作者： Bai han Mei han han WU Yuman LIN Jinjun HE Rong LIU Ordos Forestry and Grassland research Institute Ordos 017000China Ordos Meteorological Bureau Ordos 017000China Agriculture and Animal Husbandry Technology Extension Center of Etuoke Banner Ordos 017000China

[Objectives] Caragana korshinskii resources are rich in Ordos with a large planting area, but they have not been fully and reasonably utilized. This study aims to effectively convert C. korshinskii into high-quality feed, improve the quality of milk and meat of herbivores and increase production. [Methods] The utilization of C. korshinskii in feed was studied in various aspects, the factors were selected, the level was determined, and the multi-factor experiment—orthogonal experiment was conducted. [Results] The effect on the growth of sheep was in the order of silage > C. korshinskii feed > corn stalk silage > hay meal, and the effect on the growth of goats was in the order of hay meal > C. korshinskii feed > silage > corn stalk silage. [Conclusions] From the analysis of the range results, the best level combination was selected: in different growing seasons, C. korshinskii branch and leaf silage in full-bloom stage had the greatest effect on the growth of sheep and goats, and the fattening effect was the best;among the different silage methods of C. korshinskii, the C. korshinskii—corn stalk mixture silage had a good feeding effect for sheep and goats.

关键词： Forage Caragana korshinskii Silage Development and utilization

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