检索结果-南通市图书馆

A novel low-cost visual ear tag based identification system for precision beef cattle livestock farming

Information Processing in Agriculture 2024年第1期11卷 117-126页

作者： Andrea Pretto Gianpaolo Savio Flaviana Gottardo Francesca Uccheddu Gianmaria Concheri Department of Civil Environmental and Architectural EngineeringUniversity of PaduaItaly Department of animal Medicine Production and HealthUniversity of PadovaItaly Department of Industrial Engineering University of PadovaItaly

The precision livestock farming(PLF)has the objective to maximize each animal's performance while reducing the environmental impact and maintaining the quality and safety of meat *** the PLF techniques,the personalised management of each individual animal based on sensors systems,represents a viable *** is worth noting that the implementation of an effective PLF approach can be still expensive,especially for small and medium-sized farms;for this reason,to guarantee the sustainability of a customized livestock management system and encourage its use,plug and play and cost-effective systems are *** this context,we present a novel low-cost method for identifying beef cattle and recognizing their basic activities by a single surveillance *** leveraging the current state-of-the-art methods for real-time object detection,(i.e.,YOLOv3)cattle's face areas,we propose a novel mechanism able to detect the ear tag as well as the water ingestion state when the cattle is close to the *** cow IDs are read by an Optical Character Recognition(OCR)algorithm for which,an ad hoc error correction algorithm is here presented to avoid numbers misreading and correctly match the IDs to only actually present *** to the detection of the tag position,the OCR algorithm can be applied only to a specific region of interest reducing the computational cost and the time *** times for the areas are outputted as cattle activity recognition *** results demonstrate the effectiveness of our proposed method,showing a mAP@0.50 of 89%.

关键词： Precision livestock farming Deep learning Cattlei dentification Low-cost sensors Computer vision

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Abomasal infusion of branched-chain amino acids or branched-chain keto-acids alter lactation performance and liver triglycerides in fresh cows

引用

Journal of animal Science and Biotechnology 2024年第3期15卷 1145-1157页

作者： Kristen Gallagher Isabelle Bernstein Cynthia Collings David Main Ghayyoor Ahmad Sarah Naughton Jayasimha Daddam Vengai Mavangira Mike Vandehaar Zheng Zhou Department of animal Science Michigan State UniversityEast Lansing 48824USA Department of Veterinary Diagnostic and production animal Medicine Ames 50011USA

Background Dairy cows are at high risk of fatty liver disease in early lactation,but current preventative measures are not always *** with fatty liver have lower circulating branched-chain amino acid(BCAA)concentra-tions whereas cows with high circulating BCAA levels have low liver triglyceride(TG).Our objective was to determine the impact of BCAA and their corresponding ketoacids(branched-chain ketoacids,BCKA)on production performance and liver TG accumulation in Holstein cows in the first 3 weeks *** Thirty-six multiparous Holstein cows were used in a randomized block design *** were abomasally infused for the first 21 d postpartum with solutions of 1)saline(CON,n=12);2)BCA(67 g valine,50 g leu-cine,and 34 g isoleucine,n=12);and 3)BCK(77 g 2-ketovaline calcium salt,57 g 2-ketoleucine calcium salt,and 39 g 2-ketoisoleucine calcium salt,n=12).All cows received the same *** effects were determined using PROC GLIMMIX in *** No differences were detected for body weight,body condition score,or dry matter intake averaged over the first 21 d *** receiving BCK had significantly lower liver TG concentrations compared to CON(6.60%vs.4.77%,standard error of the mean(SEM)0.49)during the first 3 weeks of *** of BCA increased milk yield(39.5 vs.35.3 kg/d,SEM 1.8),milk fat yield(2.10 vs.1.69 kg/d,SEM 0.08),and lactose yield(2.11 vs.1.67 kg/d,SEM 0.07)compared with *** to CON,cows receiving BCA had lower plasma glucose(55.0 vs.59.2 mg/dL,SEM 0.86)but higherβ-hydroxybutyrate(9.17 vs.6.00 mg/dL,SEM 0.80).Conclusions Overall,BCAA supplementation in this study improved milk production,whereas BCKA supplementa-tion reduced TG accumulation in the liver of fresh cows.

关键词： Branched-chain amino acids Branched-chain ketoacids Fatty liver Fresh cow

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Hi-Tag:a simple and efficient method for identifying protein-mediated long-range chromatin interactions with low cell numbers

引用

Science China(Life Sciences) 2024年第5期67卷 1027-1034页

作者： Xiaolong Qi Lu Zhang Qiulin Zhao Peng Zhou Sai Xian Zhang Jingjin Li Zhuqing Zheng Yue Xiang Xueting Dai Zhe Jin Yaobang Jian Xinyun Li Liangliang Fu Shuhong Zhao Key Lab of Agricultural animal Genetics Breeding and Reproduction of Ministry of Education and Key Laboratory of Swine Genetics and Breeding of Ministry of AgricultureCollege of Animal Science and TechnologyHuazhong Agricultural UniversityWuhan 430070China The Cooperative Innovation Center for Sustainable Pig production Wuhan 430070China Hubei Hongshan Laboratory Frontiers Science Center for Animal Breeding and Sustainable ProductionWuhan 430070China

Protein-mediated chromatin interactions can be revealed by coupling proximity-based ligation with chromatin ***,these techniques require complex experimental procedures and millions of cells per experiment,which limits their widespread application in life science ***,we develop a novel method,Hi-Tag,that identifies high-resolution,long-range chromatin interactions through transposase tagmentation and chromatin proximity ligation(with a phosphorothioate-modified linker).Hi-Tag can be implemented using as few as 100,000 cells,involving simple experimental procedures that can be completed within 1.5 ***,Hi-Tag is capable of using its own data to identify the binding sites of specific proteins,based on which,it can acquire accurate interaction *** results suggest that Hi-Tag has great potential for advancing chromatin interaction studies,particularly in the context of limited cell availability.

关键词： Hi-Tag chromatin interactions transposase tagmentation chromatin proximity ligation low cell numbers

来源：

维普期刊数据库

同方期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The virulence regulator AbsR in avian pathogenic Escherichia coli has pleiotropic effects on bacterial physiology

引用

Journal of Integrative Agriculture 2024年第2期23卷 649-668页

作者： Dongfang Zhao Haobo Zhang Xinyang Zhang Fengwei Jiang Yijing Li Wentong Cai Ganwu Li State Key Laboratory for animal Disease Control and Prevention Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069China College of Veterinary Medicine Northeast Agricultural UniversityHarbin 150038China National animal Tuberculosis Reference Laboratory Division of Zoonoses SurveillanceChina Animal Health and Epidemiology CenterQingdao 266032China Department of Veterinary Diagnostic and production animal Medicine College of Veterinary MedicineIowa State UniversityAmesIowa 50011-1134the United States

Avian pathogenic Escherichia coli(APEC)belonging to extraintestinal pathogenic ***(ExPEC)can cause severe infections in extraintestinal tissues in birds and humans,such as the lungs and ***(microcin production regulation,locus A,herein renamed AbsR,a blood survival regulator),a member of the MarR(multiple antibiotic resistance regulator)transcriptional regulator family,governs the expression of capsule biosynthetic genes in human ExPEC and represents a promising druggable target for ***,a deep understanding of the AbsR regulatory mechanism as well as its regulon is *** this study,we present a systems-level analysis of the APEC AbsR regulon using ChIP-Seq(chromatin immunoprecipitation sequencing)and RNA-Seq(RNA sequencing)*** found that AbsR directly regulates 99 genes and indirectly regulates 667 ***,we showed that:1)AbsR contributes to antiphagocytotic effects by macrophages and virulence in a mouse model for systemic infection by directly activating the capsular gene cluster;2)AbsR positively impacts biofilm formation via direct regulation of the T2SS(type II secretion system)but plays a marginal role in virulence;and 3)AbsR directly upregulates the acid tolerance signaling system EvgAS to withstand acid stress but is dispensable in ExPEC ***,our data indicate that the role of AbsR in virulence gene regulation is relatively conserved in ExPEC ***,this study provides a comprehensive analysis of the AbsR regulon and regulatory mechanism,and our data suggest that AbsR likely influences virulence primarily through the control of capsule ***,we found that AbsR severely represses the expression of the type I-F CRISPR(clustered regularly interspaced short palindromic repeats)-Cas(CRISPR associated)systems,which could have implications in CRISPR biology and application.

关键词： avian pathogenic Escherichia coli(APEC) extraintestinal pathogenic Escherichia coli(ExPEC) AbsR RNA-Seq ChIP-Seq gene regulation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Gut microbiota intervention attenuates thermogenesis in broilers exposed to high temperature through modulation of the hypothalamic 5‑HT pathway

引用

Journal of animal Science and Biotechnology 2024年第2期15卷 679-698页

作者： Sheng Li Xiaoqing Li Kai Wang Yansen Li Kentaro Nagaoka Chunmei Li Research centre for Livestock Environmental Control and Smart production College of Animal Science and TechnologyNanjing Agricultural UniversityNanjing 210095China Laboratory of Veterinary Physiology Department of Veterinary MedicineFaculty of AgricultureTokyo University of Agriculture and TechnologyTokyo 183‑8509Japan

Background Broilers have a robust metabolism and high body temperature,which make them less tolerant to hightemperature(HT)environments and more susceptible to challenges from elevated *** microbes,functioning as symbionts within the host,possess the capacity to significantly regulate the physiological functions and environmental adaptability of the *** study aims to investigate the effects of gut microbial intervention on the body temperature and thermogenesis of broilers at different ambient temperatures,as well as the underlying mechanism involving the"gut-brain"*** Broilers were subjected to gut microbiota interference with or without antibiotics(control or ABX)starting at 1 day of *** 21 day of age,they were divided into 4 groups and exposed to different environments for 7 d:The control and ABX groups at room temperature(RT,24±1℃,60%relative humidity(RH),24 h/d)and the control-HT and ABX-HT groups at high temperature(HT,32±1℃,60%RH,24 h/d).Results The results demonstrated that the antibiotic-induced gut microbiota intervention increased body weight and improved feed conversion in broiler chickens(P<0.05).Under HT conditions,the microbiota intervention reduced the rectal temperature of broiler chickens(P<0.05),inhibited the expression of avUCP and thermogenesisrelated genes in breast muscle and liver(P<0.05),and thus decreased thermogenesis ***,the gut microbiota intervention blunted the hypothalamic‒pituitary‒adrenal axis and hypothalamic–pituitary–thyroid axis activation induced by HT *** analyzing the cecal microbiota composition of control and ABX chickens maintained under HT conditions,we found that Alistipes was enriched in control *** contrast,antibioticinduced gut microbiota intervention resulted in a decrease in the relative abundance of Alistipes(P<0.05).Moreover,this difference was accompanied by increased hypothalamic 5-hydroxytryptamine(5-HT)content and TPH2 expression(P<0.05).Conclus

关键词： 5-HT Broiler chickens Gut microbiota Thermogenesis Thermoregulation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

How neonatal diet affects the long-term development of rumination behavior,rumen fermentation and feed digestion in dairy calves fed a high milk level?

引用

animal Nutrition 2024年第1期16卷 326-337页

作者： Jianxin Xiao Tianyu Chen Rong Peng Gibson M.Alugongo Hui Yang Shuai Liu Yulin Ma Jingjun Wang Shengli Li Zhijun Cao State Key Laboratory of animal Nutrition and Feeding International Calf and Heifer OrganizationCollege of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China Key Laboratory of Low Carbon Culture and Safety production in Cattle in Sichuan Animal Nutrition InstituteSichuan Agricultural UniversityChengdu 611130China

This study was to investigate growth performance,rumination development,rumen fermentation and feed digestion in young calves provided high volumes(about 20%of calf birth weight)of milk with or without forage inclusion and how these parameters correlate with each *** after birth,160 newborn Holstein female calves(41.6±4.2 kg of initial BW)were randomly divided into 2 treatments:1)starter(CON,only starter)and 2)starter and hay(HAY,both starter and hay).The calves were fed their respective experimental diets from d 4 to 84,after which they were all introduced to similar diets until the end of the experiment on d *** had no effect on growth and structural measurements throughout the experimental ***,treatment had an effect on the other parameters,mainly during the post-weaning *** supplementation tended to reduce starter dry matter intake(P=0.05),while increasing the forage intake(P<0.01)and the feed-to-gain ratio(P<0.01).HAY calves had increased neutral detergent fiber(NDF)and physically effective NDF(peNDF)intakes(P<0.05)and tended to lower(P<0.01)starch intake compared to CON *** HAY calves had a higher rumination time(P<0.01),ruminal pH(P<0.01),and acetate-to-propionate ratio(P=0.05)compared to the CON *** correlation analysis showed that rumination time was positively related to the ruminal p H at d 84(P=0.01)and 196(P=0.02).The HAY calves had similar apparent total-tract digestibility of dry matter(DM),NDF and ether extract(EE),but lower digestibility of organic matter(OM,P=0.03),crude protein(CP,P<0.01)and starch(P<0.01)compared to those of the CON calves at week ***,there were no positive relationships between rumination time and nutrient digestibility or between rumination time per kilogram DM and nutrient *** conclusion,feeding hay to calves fed a high milk level improved rumination during the post-weaning period only,without a concomitant effect on growth performance thro

关键词： Neonatal calf Early forage feeding Rumination Rumen fermentation Nutrient digestibility

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Evaluation of the Reproduction Parameters of “Lohmann Brown” Strain Chickens Fed with Cajanus cajan Leaf Meal in the Republic of Congo

引用

Open Journal of animal Sciences 2024年第3期14卷 234-248页

作者： Richard Missoko Mabeki Alexis Jonas Ognika Dora Chérita Ekou Nathalie Myreille Ockoyi Parisse Akouango Laboratory of animal production and Biodiversity National School of Agronomy and Forestry Marien Ngouabi University Brazzaville Republic of the Congo Department of animal production and health Marien Ngouabi University Brazzaville Republic of the Congo

The objective of this study was to evaluate the reproductive parameters of “Lohmann Brown” strain chickens fed on Cajanus cajan leaves and to assess the viability of the chicks after hatching. 40 hens of the “Lohmann Brown” strain fed on Cajanus cajan leaf flour laid 219 eggs divided into four batches depending on the rate of incorporation of Cajanus cajan leaf flour in the rations (0%, 5%, 10% and 15%) were trained and introduced into the incubator. Results: the incubator indicates a hatching temperature of 38˚C to 37.2˚C and relative humidity of 60% to 70%. Hatching performance shows that: The 15% batch recorded the highest fertility rate 86.95% compared to 26.88% of the control batch. The CC 15% batch recorded the highest hatching rate 36.87. The lowest rate (17.18%) was recorded in the control batch (CC 0%). The highest embryonic mortality rate was recorded in the CC 15% batch. The lowest rate in the CC 10% batch. Concerning unfertilized eggs, the highest rate is 72.20% (CC 0%), and the lowest are (12.03%;57.42% and 66.66%) recorded in CC batches. 15%, CC 5% and CC 10% respectively. For the shell mortality rate, the highest is 18.58% recorded in the CC 15% batch. The lowest shell mortality rate is 2.07%, obtained in the control batch (CC 0%);the dust mortality rate was 0% for all chicks. The study seems to indicate that the incorporation of Cajanus cajan leaf meal up to 15% into the reproduction type ration in hens does not cause any harmful effects on the reproduction performance of laying hens.

关键词： Egg Incubation Hatching Fertility Mortality Viability

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Evaluation of the nutritional value of Artemia nauplii for European seabass(Dicentrarchus labrax L.)larvae

引用

Aquaculture and Fisheries 2024年第1期9卷 78-84页

作者： Alaa A.El-Dahhar Rashwan S.Rashwan Samy Y.EL-Zaeem Shaimaa A.Shahin Mona M.Mourad Mohammed F.El Basuini animal and Fish production Department Faculty of Agriculture-Saba BashaAlexandria UniversityAlexandria21526Egypt National Institute of Oceanography and Fisheries(NIOF) Cairo11562Egypt Department of animal production Faculty of AgricultureTanta UniversityTanta31527Egypt Faculty of Desert Agriculture King Salman International UniversitySouth Sinai46618Egypt

Adapted European seabass(Dicentrarchus labrax L.)larvae at 25 days post-hatching(dph)with a primary weight and length of 23 mg and 9.92 mm,start the weaning up to 46 dph using the weaning micro-diet(54%crude protein from fish meal,powder milk,and poultry egg)concurrently with Artemia enriched with fortification emulsions(0.6 g/L)of DHA selco®as a control group(DHAS)or Fish oil+20%Vitamin C(FOVC),or Fish oil+20%Vitamin E(FOVE),or Fish oil+10%Vit.C+10%Vit.E(FOCE),or Fish oil only(FO).At 46 dph,groups of larvae fed enriched Artemia with DHAS and FOVE exhibited the highest final body weight,weight gain,average daily gain,specific growth rate,feed intake,protein efficiency ratio,and survival%as well as the lowest feed conversion ***,larvae in FOVC displayed the lowest final body weight,total length,weight gain,average daily gain,specific growth rate,feed intake,protein efficiency ratio,and survival%as well as the highest feed conversion *** histological assessment exhibited no pathological alteration as well as an improvement in the structure with DHAS,FOVE,FOVC,and FOCE co-additives compared to *** peroxidase enzyme(GPx)recorded the highest rates(P<0.05)in groups fed Artemia supplemented with DHAS and FOVE followed by FOCE and *** the lowest record for GPx activity was noted in the FO *** conclusion,Using augmented Artemia nauplii with fish oil+vitamin E in single or in companion with vitamin C as cheap antioxidants support *** larval growth,survival,and antioxidant efficacy during the critical weaning period.

关键词： European seabass Gastrointestinal histology Antioxidants Vitamin C Vitamin E DHA

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

AP-1 and SP1 trans-activate the expression of hepatic CYP1A1 and CYP2A6 in the bioactivation of AFB1 in chicken

引用

Science China(Life Sciences) 2024年第7期67卷 1468-1478页

作者： Jiang Deng Jia-Cheng Yang Yue Feng Ze-Jing Xu Kamil Kuča Meng Liu Lv-Hui Sun State Key Laboratory of Agricultural Microbiology Hubei Hongshan LaboratoryFrontiers Science Center for Animal Breeding and Sustainable ProductionCollege of Animal Sciences and TechnologyHuazhong Agricultural UniversityWuhan 430070China Department of Chemistry Faculty of ScienceUniversity of Hradec KraloveHradec Kralove 50003Czech Republic

Dietary exposure to aflatoxin B1(AFB1)is harmful to the health and performance of domestic *** hepatic cytochrome P450s(CYPs),CYP1A1 and CYP2A6,are the primary enzymes responsible for the bioactivation of AFB1to the highly toxic exo-AFB1-8,9-epoxide(AFBO)in ***,the transcriptional regulation mechanism of these CYP genes in the liver of chicks in AFB1metabolism remains ***-luciferase reporter assay,bioinformatics and site-directed mutation results indicated that specificity protein 1(SP1)and activator protein-1(AP-1)motifs were located in the core region-1,063/-948,-606/-541 of the CYP1A1 promoter as well as-636/-595,-503/-462,-147/-1 of the CYP2A6 ***,overexpression and decoy oligodeoxynucleotide technologies demonstrated that SP1 and AP-1 were pivotal transcriptional activators regulating the promoter activity of CYP1A1 and ***,bioactivation of AFB1to AFBO could be increased by upregulation of CYP1A1 and CYP2A6 expression,which was trans-activated owing to the upregulation of AP-1,rather than SP1,stimulated by AFB1-induced reactive oxygen ***,nano-selenium could reduce ROS,downregulate AP-1 expression and then decrease the expression of CYP1A1 and CYP2A6,thus alleviating the toxicity of *** conclusion,AP-1 and SP1 played important roles in the transactivation of CYP1A1 and CYP2A6 expression and further bioactivated AFB1to AFBO in chicken liver,which could provide novel targets for the remediation of aflatoxicosis in chicks.

关键词： aflatoxin B1 CYP1A1 CYP2A6 SP1 AP-1 transcriptional activation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Ferroptosis contributes to JEV-induced neuronal damage and neuroinflammation

引用

Virologica Sinica 2024年第1期39卷 144-155页

作者： Wenjing Zhu Qi Li Yong Yin Huanchun Chen Youhui Si Bibo Zhu Shengbo Cao Zikai Zhao Jing Ye National Key Laboratory of Agricultural Microbiology Huazhong Agricultural UniversityWuhan430070China Frontiers Science Center for animal Breeding and Sustainable production Huazhong Agricultural UniversityWuhan430070China The Cooperative Innovation Center for Sustainable Pig production Huazhong Agricultural UniversityWuhan430070China Hubei Hongshan Laboratory Wuhan430070China

Ferroptosis is a newly discovered prototype of programmed cell death (PCD) driven by iron-dependent phospholipid peroxidation accumulation, and it has been linked to numerous organ injuries and degenerative pathologies. Although studies have shown that a variety of cell death processes contribute to JEV-induced neuroinflammation and neuronal injury, there is currently limited research on the specific involvement of ferroptosis. In this study, we explored the neuronal ferroptosis induced by JEV infection in vitro and in vivo. Our results indicated that JEV infection induces neuronal ferroptosis through inhibiting the function of the antioxidant system mediated by glutathione (GSH)/glutathione peroxidase 4 (GPX4), as well as by promoting lipid peroxidation mediated by yes-associated protein 1 (YAP1)/long-chain acyl-CoA synthetase 4 (ACSL4). Further analyses revealed that JEV E and prM proteins function as agonists, inducing ferroptosis. Moreover, we found that treatment with a ferroptosis inhibitor in JEV-infected mice reduces the viral titers and inflammation in the mouse brains, ultimately improving the survival rate of infected mice. In conclusion, our study unveils a critical role of ferroptosis in the pathogenesis of JEV, providing new ideas for the prevention and treatment of viral encephalitis.

关键词： Japanese encephalitisvirus(JEV) Ferroptosis Neuron Lipid peroxidation Inflammatory response

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：